Restriction map for
pFN21AE3347
(Linear) (Six-base) MAPSORT of: KIEE3347.seq Check: 9601 from: 1 to: 3081
KIEE3347.orf
With 182 enzymes: SgfI *
July 3, 2012 17:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 765 3081
Size: 765 2316
AccI GT'mk_AC
Cuts at: 0 486 3081
Size: 486 2595
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 944 2299 2344 3081
Size: 944 1355 45 737
Fragments arranged by size:
1355 944 737 45
AfeI AGC'GCT
Cuts at: 0 841 1874 3081
Size: 841 1033 1207
Fragments arranged by size:
1207 1033 841
AflIII A'CryG_T
Cuts at: 0 466 3081
Size: 466 2615
AlwNI CAG_nnn'CTG
Cuts at: 0 451 1906 3081
Size: 451 1455 1175
Fragments arranged by size:
1455 1175 451
ApaI G_GGCC'C
Cuts at: 0 303 3081
Size: 303 2778
ApoI r'AATT_y
Cuts at: 0 876 1302 1622 1738 1989 2982 3081
Size: 876 426 320 116 251 993 99
Fragments arranged by size:
993 876 426 320 251 116 99
AseI AT'TA_AT
Cuts at: 0 2774 3081
Size: 2774 307
AvaI C'yCGr_G
Cuts at: 0 1800 2090 3081
Size: 1800 290 991
Fragments arranged by size:
1800 991 290
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2843 2876 3081
Size: 2843 33 205
Fragments arranged by size:
2843 205 33
BanI G'GyrC_C
Cuts at: 0 550 1959 3011 3081
Size: 550 1409 1052 70
Fragments arranged by size:
1409 1052 550 70
BanII G_rGCy'C
Cuts at: 0 303 1359 2491 3081
Size: 303 1056 1132 590
Fragments arranged by size:
1132 1056 590 303
BbsI GAAGACnn'nnnn_
Cuts at: 0 1838 3081
Size: 1838 1243
BbvCI CC'TCA_GC
Cuts at: 0 1269 1838 1884 3081
Size: 1269 569 46 1197
Fragments arranged by size:
1269 1197 569 46
BclI T'GATC_A
Cuts at: 0 636 2142 3081
Size: 636 1506 939
Fragments arranged by size:
1506 939 636
BglII A'GATC_T
Cuts at: 0 1140 2043 3081
Size: 1140 903 1038
Fragments arranged by size:
1140 1038 903
Bme1580I G_kGCm'C
Cuts at: 0 134 303 318 2679 3081
Size: 134 169 15 2361 402
Fragments arranged by size:
2361 402 169 134 15
BmtI G_CTAG'C
Cuts at: 0 1037 3081
Size: 1037 2044
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2116 2636 3081
Size: 2116 520 445
Fragments arranged by size:
2116 520 445
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 1052 1331 1592 1806 2162 2538 3081
Size: 49 1003 279 261 214 356 376 543
Fragments arranged by size:
1003 543 376 356 279 261 214 49
Bpu10I CC'TnA_GC
Cuts at: 0 972 1123 1269 1567 1838 1884 2631 3081
Size: 972 151 146 298 271 46 747 450
Fragments arranged by size:
972 747 450 298 271 151 146 46
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1031 3081
Size: 1031 2050
BsaI GGTCTCn'nnnn_
Cuts at: 0 9 45 3081
Size: 9 36 3036
Fragments arranged by size:
3036 36 9
BsaBI GATnn'nnATC
Cuts at: 0 677 2042 3081
Size: 677 1365 1039
Fragments arranged by size:
1365 1039 677
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 25 55 1150 1180 2200 2230 2536 2566
Size: 25 30 1095 30 1020 30 306 30
Cuts at: 2566 2583 2613 3081
Size: 17 30 468
Fragments arranged by size:
1095 1020 468 306 30 30 30 30
30 25 17
BseYI C'CCAG_C
Cuts at: 0 98 2605 3081
Size: 98 2507 476
Fragments arranged by size:
2507 476 98
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 842 878 1123 3081
Size: 842 36 245 1958
Fragments arranged by size:
1958 842 245 36
BsiEI CG_ry'CG
Cuts at: 0 15 3081
Size: 15 3066
BsmI GAATG_Cn'
Cuts at: 0 1689 2279 2376 2696 3081
Size: 1689 590 97 320 385
Fragments arranged by size:
1689 590 385 320 97
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2426 3081
Size: 2426 655
Bsp1286I G_dGCh'C
Cuts at: 0 134 303 318 1359 2491 2679 3081
Size: 134 169 15 1041 1132 188 402
Fragments arranged by size:
1132 1041 402 188 169 134 15
BspHI T'CATG_A
Cuts at: 0 681 3081
Size: 681 2400
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 765 2643 3081
Size: 765 1878 438
Fragments arranged by size:
1878 765 438
BsrDI GCAATG_nn'
Cuts at: 0 1561 1767 3081
Size: 1561 206 1314
Fragments arranged by size:
1561 1314 206
BsrFI r'CCGG_y
Cuts at: 0 14 3081
Size: 14 3067
BsrGI T'GTAC_A
Cuts at: 0 1194 3081
Size: 1194 1887
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1519 1906 2452 3081
Size: 1519 387 546 629
Fragments arranged by size:
1519 629 546 387
BstEII G'GTnAC_C
Cuts at: 0 2758 3081
Size: 2758 323
BstXI CCAn_nnnn'nTGG
Cuts at: 0 265 571 3081
Size: 265 306 2510
Fragments arranged by size:
2510 306 265
BstYI r'GATC_y
Cuts at: 0 619 1140 1804 1942 2043 3081
Size: 619 521 664 138 101 1038
Fragments arranged by size:
1038 664 619 521 138 101
Bsu36I CC'TnA_GG
Cuts at: 0 2910 3081
Size: 2910 171
BtgI C'CryG_G
Cuts at: 0 193 3056 3081
Size: 193 2863 25
Fragments arranged by size:
2863 193 25
BtsI GCAGTG_nn'
Cuts at: 0 1080 2221 2735 3081
Size: 1080 1141 514 346
Fragments arranged by size:
1141 1080 514 346
DraI TTT'AAA
Cuts at: 0 275 2795 3081
Size: 275 2520 286
Fragments arranged by size:
2520 286 275
DrdI GACnn_nn'nnGTC
Cuts at: 0 933 3081
Size: 933 2148
EarI CTCTTCn'nnn_
Cuts at: 0 426 2531 3081
Size: 426 2105 550
Fragments arranged by size:
2105 550 426
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 944 1052 1331 1592 1806 2162 2299
Size: 49 895 108 279 261 214 356 137
Cuts at: 2299 2344 2538 3081
Size: 45 194 543
Fragments arranged by size:
895 543 356 279 261 214 194 137
108 49 45
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1340 2495 3081
Size: 1340 1155 586
Fragments arranged by size:
1340 1155 586
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 671 3081
Size: 671 2410
HaeII r_GCGC'y
Cuts at: 0 843 1876 3081
Size: 843 1033 1205
Fragments arranged by size:
1205 1033 843
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 25 57 643 675 1144 1176 1934 1966
Size: 25 32 586 32 469 32 758 32
Cuts at: 1966 2536 2568 3081
Size: 570 32 513
Fragments arranged by size:
758 586 570 513 469 32 32 32
32 32 25
HincII GTy'rAC
Cuts at: 0 487 3081
Size: 487 2594
HindIII A'AGCT_T
Cuts at: 0 158 1293 3081
Size: 158 1135 1788
Fragments arranged by size:
1788 1135 158
MfeI C'AATT_G
Cuts at: 0 365 3081
Size: 365 2716
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2419 3081
Size: 2419 662
MslI CAynn'nnrTG
Cuts at: 0 263 1973 3081
Size: 263 1710 1108
Fragments arranged by size:
1710 1108 263
MspA1I CmG'CkG
Cuts at: 0 114 1273 1519 1888 3081
Size: 114 1159 246 369 1193
Fragments arranged by size:
1193 1159 369 246 114
NcoI C'CATG_G
Cuts at: 0 3056 3081
Size: 3056 25
NdeI CA'TA_TG
Cuts at: 0 633 1970 3081
Size: 633 1337 1111
Fragments arranged by size:
1337 1111 633
NheI G'CTAG_C
Cuts at: 0 1033 3081
Size: 1033 2048
NspI r_CATG'y
Cuts at: 0 470 509 3081
Size: 470 39 2572
Fragments arranged by size:
2572 470 39
PciI A'CATG_T
Cuts at: 0 466 3081
Size: 466 2615
PfoI T'CCnGG_A
Cuts at: 0 881 1011 3081
Size: 881 130 2070
Fragments arranged by size:
2070 881 130
PshAI GACnn'nnGTC
Cuts at: 0 2815 3081
Size: 2815 266
PsiI TTA'TAA
Cuts at: 0 398 1754 3081
Size: 398 1356 1327
Fragments arranged by size:
1356 1327 398
PspOMI G'GGCC_C
Cuts at: 0 299 3081
Size: 299 2782
PstI C_TGCA'G
Cuts at: 0 142 1524 1550 1902 2730 3081
Size: 142 1382 26 352 828 351
Fragments arranged by size:
1382 828 352 351 142 26
PvuII CAG'CTG
Cuts at: 0 1273 1519 1888 3081
Size: 1273 246 369 1193
Fragments arranged by size:
1273 1193 369 246
SalI G'TCGA_C
Cuts at: 0 485 3081
Size: 485 2596
ScaI AGT'ACT
Cuts at: 0 1812 3081
Size: 1812 1269
SfcI C'TryA_G
Cuts at: 0 138 785 1337 1508 1520 1546 1898 2726
Size: 138 647 552 171 12 26 352 828
Cuts at: 2726 3081
Size: 355
Fragments arranged by size:
828 647 552 355 352 171 138 26
12
SmlI C'TyrA_G
Cuts at: 0 1046 3081
Size: 1046 2035
StyI C'CwwG_G
Cuts at: 0 3056 3081
Size: 3056 25
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2722 2863 3081
Size: 2722 141 218
Fragments arranged by size:
2722 218 141
TatI w'GTAC_w
Cuts at: 0 1194 1810 3003 3081
Size: 1194 616 1193 78
Fragments arranged by size:
1194 1193 616 78
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1007 1039 1085 1117 2200 2232 3081
Size: 1007 32 46 32 1083 32 849
Fragments arranged by size:
1083 1007 849 46 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 37 3081
Size: 37 3044
XmnI GAAnn'nnTTC
Cuts at: 0 2357 3081
Size: 2357 724
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AlwNI ApaI ApoI
AseI AvaI BaeI BanI BanII BbsI BbvCI BclI
BglII Bme1580I BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaBI BsaXI BseYI BsgI BsiEI BsmI BsmBI Bsp1286I
BspHI BspMI BsrDI BsrFI BsrGI BstAPI BstEII BstXI
BstYI Bsu36I BtgI BtsI DraI DrdI EarI Eco57MI
EcoNI FalI HaeII Hin4I HincII HindIII MfeI MmeI
MslI MspA1I NcoI NdeI NheI NspI PciI PfoI
PshAI PsiI PspOMI PstI PvuII SalI ScaI SfcI
SmlI StyI TaqII TatI TstI Tth111I XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AhdI AleI AlfI
AloI ApaLI AscI AsiSI AvrII BamHI BbeI BcgI
BciVI BfrBI BglI BlpI BmgBI BmrI BsaAI BsaHI
BsaWI BseRI BsiHKAI BsiWI BspEI BsrBI BssHII BssSI
BstBI BstZ17I BtgZI ClaI CspCI DraIII EaeI EagI
EciI EcoICRI EcoO109I EcoRI EcoRV FseI FspI FspAI
HpaI KasI KpnI MluI MscI NaeI NarI NgoMIV
NotI NruI NsiI PacI PasI PflMI PmeI PmlI
PpiI PpuMI PspXI PsrI PvuI RsrII SacI SacII
SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
XbaI XcmI XhoI XmaI ZraI