Restriction map for
pFN21AE3339
(Linear) (Six-base) MAPSORT of: KIEE3339.seq Check: 4627 from: 1 to: 2928
KIEE3339.orf
With 182 enzymes: SgfI *
July 3, 2012 17:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1212 2928
Size: 1212 1716
Acc65I G'GTAC_C
Cuts at: 0 1475 2525 2928
Size: 1475 1050 403
Fragments arranged by size:
1475 1050 403
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1807 2405 2611 2928
Size: 1807 598 206 317
Fragments arranged by size:
1807 598 317 206
AfeI AGC'GCT
Cuts at: 0 508 607 2928
Size: 508 99 2321
Fragments arranged by size:
2321 508 99
AflIII A'CryG_T
Cuts at: 0 191 2928
Size: 191 2737
AgeI A'CCGG_T
Cuts at: 0 2528 2928
Size: 2528 400
AhdI GACnn_n'nnGTC
Cuts at: 0 477 2928
Size: 477 2451
AleI CACnn'nnGTG
Cuts at: 0 1759 2928
Size: 1759 1169
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 920 2666 2928
Size: 920 1746 262
Fragments arranged by size:
1746 920 262
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 283 315 2928
Size: 283 32 2613
Fragments arranged by size:
2613 283 32
AlwNI CAG_nnn'CTG
Cuts at: 0 171 2383 2928
Size: 171 2212 545
Fragments arranged by size:
2212 545 171
ApaI G_GGCC'C
Cuts at: 0 973 1145 1188 2928
Size: 973 172 43 1740
Fragments arranged by size:
1740 973 172 43
ApaLI G'TGCA_C
Cuts at: 0 728 1168 2928
Size: 728 440 1760
Fragments arranged by size:
1760 728 440
ApoI r'AATT_y
Cuts at: 0 2107 2928
Size: 2107 821
AvaI C'yCGr_G
Cuts at: 0 60 561 961 1187 1847 2764 2881 2928
Size: 60 501 400 226 660 917 117 47
Fragments arranged by size:
917 660 501 400 226 117 60 47
BamHI G'GATC_C
Cuts at: 0 132 2735 2928
Size: 132 2603 193
Fragments arranged by size:
2603 193 132
BanI G'GyrC_C
Cuts at: 0 565 768 782 1475 2525 2885 2928
Size: 565 203 14 693 1050 360 43
Fragments arranged by size:
1050 693 565 360 203 43 14
BanII G_rGCy'C
Cuts at: 0 960 973 1145 1188 1196 1719 2127 2573
Size: 960 13 172 43 8 523 408 446
Cuts at: 2573 2928
Size: 355
Fragments arranged by size:
960 523 446 408 355 172 43 13
8
BbsI GAAGACnn'nnnn_
Cuts at: 0 588 2928
Size: 588 2340
BbvCI CC'TCA_GC
Cuts at: 0 2849 2928
Size: 2849 79
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1804 1838 2928
Size: 1804 34 1090
Fragments arranged by size:
1804 1090 34
BclI T'GATC_A
Cuts at: 0 1587 2096 2928
Size: 1587 509 832
Fragments arranged by size:
1587 832 509
BglI GCCn_nnn'nGGC
Cuts at: 0 21 574 755 845 2723 2928
Size: 21 553 181 90 1878 205
Fragments arranged by size:
1878 553 205 181 90 21
BlpI GC'TnA_GC
Cuts at: 0 2458 2928
Size: 2458 470
Bme1580I G_kGCm'C
Cuts at: 0 732 892 973 1126 1145 1172 1188 2604
Size: 732 160 81 153 19 27 16 1416
Cuts at: 2604 2888 2928
Size: 284 40
Fragments arranged by size:
1416 732 284 160 153 81 40 27
19 16
BmrI ACTGGGnnnn_n'
Cuts at: 0 1107 1573 2928
Size: 1107 466 1355
Fragments arranged by size:
1355 1107 466
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 528 2928
Size: 528 2400
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1652 1789 2659 2857 2928
Size: 1652 137 870 198 71
Fragments arranged by size:
1652 870 198 137 71
Bpu10I CC'TnA_GC
Cuts at: 0 1015 2849 2928
Size: 1015 1834 79
Fragments arranged by size:
1834 1015 79
BsaI GGTCTCn'nnnn_
Cuts at: 0 352 464 610 2928
Size: 352 112 146 2318
Fragments arranged by size:
2318 352 146 112
BsaAI yAC'GTr
Cuts at: 0 288 887 1452 2928
Size: 288 599 565 1476
Fragments arranged by size:
1476 599 565 288
BsaBI GATnn'nnATC
Cuts at: 0 1763 2095 2365 2869 2928
Size: 1763 332 270 504 59
Fragments arranged by size:
1763 504 332 270 59
BsaHI Gr'CG_yC
Cuts at: 0 1080 2069 2928
Size: 1080 989 859
Fragments arranged by size:
1080 989 859
BsaWI w'CCGG_w
Cuts at: 0 854 1703 2528 2928
Size: 854 849 825 400
Fragments arranged by size:
854 849 825 400
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 283 313 1628 1658 2647 2677 2928
Size: 283 30 1315 30 989 30 251
Fragments arranged by size:
1315 989 283 251 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 283 1649 2928
Size: 283 1366 1279
Fragments arranged by size:
1366 1279 283
BseYI C'CCAG_C
Cuts at: 0 117 664 893 1062 1281 1718 1936 2585
Size: 117 547 229 169 219 437 218 649
Cuts at: 2585 2713 2809 2928
Size: 128 96 119
Fragments arranged by size:
649 547 437 229 219 218 169 128
119 117 96
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1432 1694 1808 2779 2928
Size: 1432 262 114 971 149
Fragments arranged by size:
1432 971 262 149 114
BsiHKAI G_wGCw'C
Cuts at: 0 732 904 960 1172 2573 2928
Size: 732 172 56 212 1401 355
Fragments arranged by size:
1401 732 355 212 172 56
BsmI GAATG_Cn'
Cuts at: 0 401 1556 2897 2928
Size: 401 1155 1341 31
Fragments arranged by size:
1341 1155 401 31
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2757 2928
Size: 2757 171
Bsp1286I G_dGCh'C
Cuts at: 0 732 892 904 960 973 1126 1145 1172
Size: 732 160 12 56 13 153 19 27
Cuts at: 1172 1188 1196 1719 2127 2573 2604 2888 2928
Size: 16 8 523 408 446 31 284 40
Fragments arranged by size:
732 523 446 408 284 160 153 56
40 31 27 19 16 13 12 8
BspHI T'CATG_A
Cuts at: 0 1590 2093 2573 2928
Size: 1590 503 480 355
Fragments arranged by size:
1590 503 480 355
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 697 1212 2928
Size: 697 515 1716
Fragments arranged by size:
1716 697 515
BsrBI CCG'CTC
Cuts at: 0 6 944 2148 2928
Size: 6 938 1204 780
Fragments arranged by size:
1204 938 780 6
BsrDI GCAATG_nn'
Cuts at: 0 2390 2675 2928
Size: 2390 285 253
Fragments arranged by size:
2390 285 253
BsrFI r'CCGG_y
Cuts at: 0 2528 2606 2928
Size: 2528 78 322
Fragments arranged by size:
2528 322 78
BsrGI T'GTAC_A
Cuts at: 0 188 374 2928
Size: 188 186 2554
Fragments arranged by size:
2554 188 186
BssHII G'CGCG_C
Cuts at: 0 67 69 2928
Size: 67 2 2859
Fragments arranged by size:
2859 67 2
BstAPI GCAn_nnn'nTGC
Cuts at: 0 743 832 2055 2903 2928
Size: 743 89 1223 848 25
Fragments arranged by size:
1223 848 743 89 25
BstEII G'GTnAC_C
Cuts at: 0 432 2928
Size: 432 2496
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1156 1761 2809 2928
Size: 1156 605 1048 119
Fragments arranged by size:
1156 1048 605 119
BstYI r'GATC_y
Cuts at: 0 132 254 2360 2402 2735 2928
Size: 132 122 2106 42 333 193
Fragments arranged by size:
2106 333 193 132 122 42
Bsu36I CC'TnA_GG
Cuts at: 0 723 1029 1927 2140 2928
Size: 723 306 898 213 788
Fragments arranged by size:
898 788 723 306 213
BtgI C'CryG_G
Cuts at: 0 261 437 534 2822 2928
Size: 261 176 97 2288 106
Fragments arranged by size:
2288 261 176 106 97
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2435 2928
Size: 2435 493
BtsI GCAGTG_nn'
Cuts at: 0 741 822 902 1214 2875 2928
Size: 741 81 80 312 1661 53
Fragments arranged by size:
1661 741 312 81 80 53
DrdI GACnn_nn'nnGTC
Cuts at: 0 2161 2928
Size: 2161 767
EaeI y'GGCC_r
Cuts at: 0 945 1293 1958 1992 2180 2608 2928
Size: 945 348 665 34 188 428 320
Fragments arranged by size:
945 665 428 348 320 188 34
EarI CTCTTCn'nnn_
Cuts at: 0 2503 2708 2928
Size: 2503 205 220
Fragments arranged by size:
2503 220 205
EcoICRI GAG'CTC
Cuts at: 0 958 2571 2928
Size: 958 1613 357
Fragments arranged by size:
1613 958 357
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1652 1789 1807 2405 2611 2659 2857 2928
Size: 1652 137 18 598 206 48 198 71
Fragments arranged by size:
1652 598 206 198 137 71 48 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2014 2139 2928
Size: 2014 125 789
Fragments arranged by size:
2014 789 125
EcoO109I rG'GnC_Cy
Cuts at: 0 686 719 969 970 991 1141 1142 1184
Size: 686 33 250 1 21 150 1 42
Cuts at: 1184 1406 1567 1623 1818 1922 2244 2853 2928
Size: 222 161 56 195 104 322 609 75
Fragments arranged by size:
686 609 322 250 222 195 161 150
104 75 56 42 33 21 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 378 2928
Size: 378 2550
HaeII r_GCGC'y
Cuts at: 0 12 510 609 2928
Size: 12 498 99 2319
Fragments arranged by size:
2319 498 99 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 463 495 1327 1359 2345 2377 2478 2510
Size: 463 32 832 32 986 32 101 32
Cuts at: 2510 2727 2759 2928
Size: 217 32 169
Fragments arranged by size:
986 832 463 217 169 101 32 32
32 32 32
HindIII A'AGCT_T
Cuts at: 0 2623 2928
Size: 2623 305
KpnI G_GTAC'C
Cuts at: 0 1479 2529 2928
Size: 1479 1050 399
Fragments arranged by size:
1479 1050 399
MfeI C'AATT_G
Cuts at: 0 249 2928
Size: 249 2679
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 435 2353 2928
Size: 435 1918 575
Fragments arranged by size:
1918 575 435
MscI TGG'CCA
Cuts at: 0 1295 1960 1994 2182 2928
Size: 1295 665 34 188 746
Fragments arranged by size:
1295 746 665 188 34
MslI CAynn'nnrTG
Cuts at: 0 1217 1321 1759 2231 2266 2776 2928
Size: 1217 104 438 472 35 510 152
Fragments arranged by size:
1217 510 472 438 152 104 35
MspA1I CmG'CkG
Cuts at: 0 54 263 664 1722 2160 2848 2928
Size: 54 209 401 1058 438 688 80
Fragments arranged by size:
1058 688 438 401 209 80 54
NaeI GCC'GGC
Cuts at: 0 2608 2928
Size: 2608 320
NcoI C'CATG_G
Cuts at: 0 2822 2928
Size: 2822 106
NdeI CA'TA_TG
Cuts at: 0 2592 2928
Size: 2592 336
NgoMIV G'CCGG_C
Cuts at: 0 2606 2928
Size: 2606 322
NspI r_CATG'y
Cuts at: 0 195 741 830 986 1216 1333 2928
Size: 195 546 89 156 230 117 1595
Fragments arranged by size:
1595 546 230 195 156 117 89
PasI CC'CwG_GG
Cuts at: 0 18 328 1423 1496 2167 2349 2693 2928
Size: 18 310 1095 73 671 182 344 235
Fragments arranged by size:
1095 671 344 310 235 182 73 18
PciI A'CATG_T
Cuts at: 0 191 2928
Size: 191 2737
PflMI CCAn_nnn'nTGG
Cuts at: 0 145 396 2928
Size: 145 251 2532
Fragments arranged by size:
2532 251 145
PfoI T'CCnGG_A
Cuts at: 0 1708 2318 2928
Size: 1708 610 610
Fragments arranged by size:
1708 610 610
PmlI CAC'GTG
Cuts at: 0 288 887 1452 2928
Size: 288 599 565 1476
Fragments arranged by size:
1476 599 565 288
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 283 315 2928
Size: 283 32 2613
Fragments arranged by size:
2613 283 32
PpuMI rG'GwC_Cy
Cuts at: 0 686 991 1406 1567 1818 1922 2244 2853
Size: 686 305 415 161 251 104 322 609
Cuts at: 2853 2928
Size: 75
Fragments arranged by size:
686 609 415 322 305 251 161 104
75
PspOMI G'GGCC_C
Cuts at: 0 969 1141 1184 2928
Size: 969 172 43 1744
Fragments arranged by size:
1744 969 172 43
PspXI vC'TCGA_Gb
Cuts at: 0 2764 2928
Size: 2764 164
PstI C_TGCA'G
Cuts at: 0 299 450 748 1209 1520 2192 2928
Size: 299 151 298 461 311 672 736
Fragments arranged by size:
736 672 461 311 299 298 151
PvuII CAG'CTG
Cuts at: 0 2160 2928
Size: 2160 768
SacI G_AGCT'C
Cuts at: 0 960 2573 2928
Size: 960 1613 355
Fragments arranged by size:
1613 960 355
SacII CC_GC'GG
Cuts at: 0 264 2928
Size: 264 2664
SanDI GG'GwC_CC
Cuts at: 0 1406 1922 2928
Size: 1406 516 1006
Fragments arranged by size:
1406 1006 516
SapI GCTCTTCn'nnn_
Cuts at: 0 2503 2928
Size: 2503 425
SfcI C'TryA_G
Cuts at: 0 295 446 744 773 1205 1516 2188 2928
Size: 295 151 298 29 432 311 672 740
Fragments arranged by size:
740 672 432 311 298 295 151 29
SmaI CCC'GGG
Cuts at: 0 62 563 963 1189 1849 2883 2928
Size: 62 501 400 226 660 1034 45
Fragments arranged by size:
1034 660 501 400 226 62 45
SmlI C'TyrA_G
Cuts at: 0 2764 2928
Size: 2764 164
SphI G_CATG'C
Cuts at: 0 741 986 1333 2928
Size: 741 245 347 1595
Fragments arranged by size:
1595 741 347 245
SrfI GCCC'GGGC
Cuts at: 0 2883 2928
Size: 2883 45
StuI AGG'CCT
Cuts at: 0 1349 2928
Size: 1349 1579
StyI C'CwwG_G
Cuts at: 0 74 1835 2822 2928
Size: 74 1761 987 106
Fragments arranged by size:
1761 987 106 74
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 137 2928
Size: 137 2791
TatI w'GTAC_w
Cuts at: 0 188 374 2928
Size: 188 186 2554
Fragments arranged by size:
2554 188 186
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1765 1797 2928
Size: 1765 32 1131
Fragments arranged by size:
1765 1131 32
Tth111I GACn'n_nGTC
Cuts at: 0 1882 2928
Size: 1882 1046
XbaI T'CTAG_A
Cuts at: 0 1488 2928
Size: 1488 1440
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 146 793 1994 2164 2262 2928
Size: 146 647 1201 170 98 666
Fragments arranged by size:
1201 666 647 170 146 98
XhoI C'TCGA_G
Cuts at: 0 2764 2928
Size: 2764 164
XmaI C'CCGG_G
Cuts at: 0 60 561 961 1187 1847 2881 2928
Size: 60 501 400 226 660 1034 47
Fragments arranged by size:
1034 660 501 400 226 60 47
Enzymes that do cut:
AarI Acc65I AcuI AfeI AflIII AgeI AhdI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AvaI BamHI
BanI BanII BbsI BbvCI BcgI BclI BglI BlpI
Bme1580I BmrI BplI BpmI Bpu10I BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I
FalI HaeII Hin4I HindIII KpnI MfeI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NspI PasI
PciI PflMI PfoI PmlI PpiI PpuMI PspOMI PspXI
PstI PvuII SacI SacII SanDI SapI SfcI SmaI
SmlI SphI SrfI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AccI AclI AflII AscI AseI AsiSI AvrII
BaeI BbeI BciVI BfrBI BglII BmgBI BmtI BpuEI
BsiEI BsiWI BspEI BssSI BstBI BstZ17I ClaI CspCI
DraI DraIII EagI EciI EcoRI EcoRV FseI FspI
FspAI HincII HpaI KasI MluI NarI NheI NotI
NruI NsiI PacI PmeI PshAI PsiI PsrI PvuI
RsrII SalI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SnaBI SpeI SspI SwaI XmnI ZraI