Restriction map for
pFN21AE3338
(Linear) (Six-base) MAPSORT of: KIEE3338.seq Check: 4841 from: 1 to: 2925
KIEE3338.orf
With 182 enzymes: SgfI *
March 22, 2012 16:30 ..
AclI AA'CG_TT
Cuts at: 0 327 2925
Size: 327 2598
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 700 1108 1328 2392 2925
Size: 700 408 220 1064 533
Fragments arranged by size:
1064 700 533 408 220
AflIII A'CryG_T
Cuts at: 0 528 2624 2925
Size: 528 2096 301
Fragments arranged by size:
2096 528 301
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 64 1403 2925
Size: 64 1339 1522
Fragments arranged by size:
1522 1339 64
AlwNI CAG_nnn'CTG
Cuts at: 0 406 660 1618 2925
Size: 406 254 958 1307
Fragments arranged by size:
1307 958 406 254
ApoI r'AATT_y
Cuts at: 0 839 897 1372 2277 2925
Size: 839 58 475 905 648
Fragments arranged by size:
905 839 648 475 58
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 276 297 309 330 2925
Size: 276 21 12 21 2595
Fragments arranged by size:
2595 276 21 21 12
BanI G'GyrC_C
Cuts at: 0 40 1231 2893 2925
Size: 40 1191 1662 32
Fragments arranged by size:
1662 1191 40 32
BanII G_rGCy'C
Cuts at: 0 602 2646 2925
Size: 602 2044 279
Fragments arranged by size:
2044 602 279
BbsI GAAGACnn'nnnn_
Cuts at: 0 530 1279 2225 2380 2811 2845 2925
Size: 530 749 946 155 431 34 80
Fragments arranged by size:
946 749 530 431 155 80 34
BbvCI CC'TCA_GC
Cuts at: 0 1300 1363 1641 2925
Size: 1300 63 278 1284
Fragments arranged by size:
1300 1284 278 63
BglII A'GATC_T
Cuts at: 0 708 2025 2925
Size: 708 1317 900
Fragments arranged by size:
1317 900 708
Bme1580I G_kGCm'C
Cuts at: 0 1554 2925
Size: 1554 1371
BmrI ACTGGGnnnn_n'
Cuts at: 0 1506 1560 1629 2925
Size: 1506 54 69 1296
Fragments arranged by size:
1506 1296 69 54
BmtI G_CTAG'C
Cuts at: 0 2155 2925
Size: 2155 770
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 376 681 2673 2925
Size: 376 305 1992 252
Fragments arranged by size:
1992 376 305 252
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 50 309 358 820 1057 1118 1432 2572
Size: 50 259 49 462 237 61 314 1140
Cuts at: 2572 2698 2925
Size: 126 227
Fragments arranged by size:
1140 462 314 259 237 227 126 61
50 49
Bpu10I CC'TnA_GC
Cuts at: 0 1300 1363 1641 1820 2925
Size: 1300 63 278 179 1105
Fragments arranged by size:
1300 1105 278 179 63
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1015 1882 2026 2121 2925
Size: 1015 867 144 95 804
Fragments arranged by size:
1015 867 804 144 95
BsaI GGTCTCn'nnnn_
Cuts at: 0 347 2674 2925
Size: 347 2327 251
Fragments arranged by size:
2327 347 251
BsaBI GATnn'nnATC
Cuts at: 0 413 2906 2925
Size: 413 2493 19
Fragments arranged by size:
2493 413 19
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1615 1645 2416 2446 2925
Size: 1615 30 771 30 479
Fragments arranged by size:
1615 771 479 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1012 1075 2137 2173 2359 2925
Size: 1012 63 1062 36 186 566
Fragments arranged by size:
1062 1012 566 186 63 36
BseYI C'CCAG_C
Cuts at: 0 2390 2466 2522 2925
Size: 2390 76 56 403
Fragments arranged by size:
2390 403 76 56
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 673 1127 2925
Size: 673 454 1798
Fragments arranged by size:
1798 673 454
BsiHKAI G_wGCw'C
Cuts at: 0 224 602 2925
Size: 224 378 2323
Fragments arranged by size:
2323 378 224
BsmI GAATG_Cn'
Cuts at: 0 990 2925
Size: 990 1935
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1030 2925
Size: 1030 1895
Bsp1286I G_dGCh'C
Cuts at: 0 224 602 1554 2646 2925
Size: 224 378 952 1092 279
Fragments arranged by size:
1092 952 378 279 224
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 827 2925
Size: 827 2098
BsrBI CCG'CTC
Cuts at: 0 925 2925
Size: 925 2000
BsrDI GCAATG_nn'
Cuts at: 0 2516 2925
Size: 2516 409
BssSI C'ACGA_G
Cuts at: 0 785 2925
Size: 785 2140
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2256 2925
Size: 2256 669
BstBI TT'CG_AA
Cuts at: 0 843 2925
Size: 843 2082
BstYI r'GATC_y
Cuts at: 0 708 2025 2046 2108 2315 2925
Size: 708 1317 21 62 207 610
Fragments arranged by size:
1317 708 610 207 62 21
BtgI C'CryG_G
Cuts at: 0 2156 2925
Size: 2156 769
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 712 2893 2925
Size: 712 2181 32
Fragments arranged by size:
2181 712 32
BtsI GCAGTG_nn'
Cuts at: 0 148 656 829 1115 2123 2925
Size: 148 508 173 286 1008 802
Fragments arranged by size:
1008 802 508 286 173 148
EaeI y'GGCC_r
Cuts at: 0 485 2159 2925
Size: 485 1674 766
Fragments arranged by size:
1674 766 485
EarI CTCTTCn'nnn_
Cuts at: 0 126 497 2330 2411 2726 2925
Size: 126 371 1833 81 315 199
Fragments arranged by size:
1833 371 315 199 126 81
EcoICRI GAG'CTC
Cuts at: 0 600 2925
Size: 600 2325
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 50 309 358 700 820 1057 1108 1118
Size: 50 259 49 342 120 237 51 10
Cuts at: 1118 1328 1432 2392 2572 2698 2925
Size: 210 104 960 180 126 227
Fragments arranged by size:
960 342 259 237 227 210 180 126
120 104 51 50 49 10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 676 2925
Size: 676 2249
EcoO109I rG'GnC_Cy
Cuts at: 0 1408 1479 1581 2477 2925
Size: 1408 71 102 896 448
Fragments arranged by size:
1408 896 448 102 71
EcoRI G'AATT_C
Cuts at: 0 897 2925
Size: 897 2028
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 229 2772 2925
Size: 229 2543 153
Fragments arranged by size:
2543 229 153
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 247 279 496 498 528 530 589 621
Size: 247 32 217 2 30 2 59 32
Cuts at: 621 779 811 1612 1615 1644 1647 1990 2022
Size: 158 32 801 3 29 3 343 32
Cuts at: 2022 2925
Size: 903
Fragments arranged by size:
903 801 343 247 217 158 59 32
32 32 32 30 29 3 3 2
2
HincII GTy'rAC
Cuts at: 0 279 2366 2925
Size: 279 2087 559
Fragments arranged by size:
2087 559 279
MfeI C'AATT_G
Cuts at: 0 1882 2254 2925
Size: 1882 372 671
Fragments arranged by size:
1882 671 372
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 164 2000 2925
Size: 164 1836 925
Fragments arranged by size:
1836 925 164
MscI TGG'CCA
Cuts at: 0 487 2161 2925
Size: 487 1674 764
Fragments arranged by size:
1674 764 487
MslI CAynn'nnrTG
Cuts at: 0 46 1205 1847 2925
Size: 46 1159 642 1078
Fragments arranged by size:
1159 1078 642 46
MspA1I CmG'CkG
Cuts at: 0 28 70 192 519 1467 1986 1996 2925
Size: 28 42 122 327 948 519 10 929
Fragments arranged by size:
948 929 519 327 122 42 28 10
NcoI C'CATG_G
Cuts at: 0 2156 2925
Size: 2156 769
NheI G'CTAG_C
Cuts at: 0 2151 2925
Size: 2151 774
NspI r_CATG'y
Cuts at: 0 1152 2226 2628 2781 2925
Size: 1152 1074 402 153 144
Fragments arranged by size:
1152 1074 402 153 144
PciI A'CATG_T
Cuts at: 0 2624 2925
Size: 2624 301
PfoI T'CCnGG_A
Cuts at: 0 607 797 2925
Size: 607 190 2128
Fragments arranged by size:
2128 607 190
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 845 877 1964 1996 2925
Size: 845 32 1087 32 929
Fragments arranged by size:
1087 929 845 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 1479 1581 2477 2925
Size: 1479 102 896 448
Fragments arranged by size:
1479 896 448 102
PsiI TTA'TAA
Cuts at: 0 911 2925
Size: 911 2014
PstI C_TGCA'G
Cuts at: 0 524 824 1562 2925
Size: 524 300 738 1363
Fragments arranged by size:
1363 738 524 300
PvuII CAG'CTG
Cuts at: 0 28 70 519 1467 1986 1996 2925
Size: 28 42 449 948 519 10 929
Fragments arranged by size:
948 929 519 449 42 28 10
SacI G_AGCT'C
Cuts at: 0 602 2925
Size: 602 2323
ScaI AGT'ACT
Cuts at: 0 1575 2925
Size: 1575 1350
SexAI A'CCwGG_T
Cuts at: 0 1253 2357 2925
Size: 1253 1104 568
Fragments arranged by size:
1253 1104 568
SfcI C'TryA_G
Cuts at: 0 520 694 820 1186 1558 2140 2395 2925
Size: 520 174 126 366 372 582 255 530
Fragments arranged by size:
582 530 520 372 366 255 174 126
SmlI C'TyrA_G
Cuts at: 0 994 1897 2041 2100 2925
Size: 994 903 144 59 825
Fragments arranged by size:
994 903 825 144 59
SphI G_CATG'C
Cuts at: 0 1152 2925
Size: 1152 1773
StuI AGG'CCT
Cuts at: 0 2212 2925
Size: 2212 713
StyI C'CwwG_G
Cuts at: 0 2156 2925
Size: 2156 769
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 449 2925
Size: 449 2476
TatI w'GTAC_w
Cuts at: 0 1573 2925
Size: 1573 1352
XbaI T'CTAG_A
Cuts at: 0 2659 2925
Size: 2659 266
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 504 2925
Size: 504 2421
XmnI GAAnn'nnTTC
Cuts at: 0 667 1313 2925
Size: 667 646 1612
Fragments arranged by size:
1612 667 646
Enzymes that do cut:
AclI AcuI AflIII AlfI AlwNI ApoI BaeI BanI
BanII BbsI BbvCI BglII Bme1580I BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaBI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI EaeI
EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI Hin4I
HincII MfeI MmeI MscI MslI MspA1I NcoI NheI
NspI PciI PfoI PpiI PpuMI PsiI PstI PvuII
SacI ScaI SexAI SfcI SmlI SphI StuI StyI
TaqII TatI XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AgeI AhdI
AleI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BamHI BbeI BcgI BciVI BclI BfrBI BglI
BlpI BmgBI BsaAI BsaHI BsaWI BsiEI BsiWI BspEI
BspHI BsrFI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraI DraIII DrdI EagI EciI EcoRV
FseI FspI FspAI HaeII HindIII HpaI KasI KpnI
MluI NaeI NarI NdeI NgoMIV NotI NruI NsiI
PacI PasI PflMI PmeI PmlI PshAI PspOMI PspXI
PsrI PvuI RsrII SacII SalI SanDI SapI SbfI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
SspI SwaI TstI Tth111I XhoI XmaI ZraI