Restriction map for
pFN21AE3336
(Linear) (Six-base) MAPSORT of: KIEE3336.seq Check: 370 from: 1 to: 2922
KIEE3336.orf
With 182 enzymes: SgfI *
March 22, 2012 16:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 10 765 2774 2922
Size: 10 755 2009 148
Fragments arranged by size:
2009 755 148 10
AccI GT'mk_AC
Cuts at: 0 728 1289 2540 2922
Size: 728 561 1251 382
Fragments arranged by size:
1251 728 561 382
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 149 453 750 763 1952 2922
Size: 149 304 297 13 1189 970
Fragments arranged by size:
1189 970 304 297 149 13
AfeI AGC'GCT
Cuts at: 0 367 2922
Size: 367 2555
AflIII A'CryG_T
Cuts at: 0 381 2922
Size: 381 2541
AleI CACnn'nnGTG
Cuts at: 0 1747 2922
Size: 1747 1175
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2497 2722 2782 2922
Size: 2497 225 60 140
Fragments arranged by size:
2497 225 140 60
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1336 1368 2922
Size: 1336 32 1554
Fragments arranged by size:
1554 1336 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1733 1950 2622 2922
Size: 1733 217 672 300
Fragments arranged by size:
1733 672 300 217
ApaI G_GGCC'C
Cuts at: 0 845 1976 2832 2900 2922
Size: 845 1131 856 68 22
Fragments arranged by size:
1131 856 845 68 22
ApaLI G'TGCA_C
Cuts at: 0 243 2922
Size: 243 2679
ApoI r'AATT_y
Cuts at: 0 100 2922
Size: 100 2822
AvaI C'yCGr_G
Cuts at: 0 33 1330 1475 1524 1847 2821 2842 2922
Size: 33 1297 145 49 323 974 21 80
Fragments arranged by size:
1297 974 323 145 80 49 33 21
BanI G'GyrC_C
Cuts at: 0 43 53 427 1362 1602 1612 2114 2916
Size: 43 10 374 935 240 10 502 802
Cuts at: 2916 2922
Size: 6
Fragments arranged by size:
935 802 502 374 240 43 10 10
6
BanII G_rGCy'C
Cuts at: 0 91 501 845 1001 1349 1567 1582 1976
Size: 91 410 344 156 348 218 15 394
Cuts at: 1976 2832 2900 2922
Size: 856 68 22
Fragments arranged by size:
856 410 394 348 344 218 156 91
68 22 15
BbeI G_GCGC'C
Cuts at: 0 2118 2922
Size: 2118 804
BbsI GAAGACnn'nnnn_
Cuts at: 0 306 347 2922
Size: 306 41 2575
Fragments arranged by size:
2575 306 41
BbvCI CC'TCA_GC
Cuts at: 0 619 1885 2922
Size: 619 1266 1037
Fragments arranged by size:
1266 1037 619
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1635 1669 2922
Size: 1635 34 1253
Fragments arranged by size:
1635 1253 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1005 2922
Size: 1005 1917
BglII A'GATC_T
Cuts at: 0 2570 2922
Size: 2570 352
BlpI GC'TnA_GC
Cuts at: 0 2881 2922
Size: 2881 41
Bme1580I G_kGCm'C
Cuts at: 0 247 845 1976 2832 2900 2921 2922
Size: 247 598 1131 856 68 21 1
Fragments arranged by size:
1131 856 598 247 68 21 1
BmgBI CAC'GTC
Cuts at: 0 639 1749 2922
Size: 639 1110 1173
Fragments arranged by size:
1173 1110 639
BmrI ACTGGGnnnn_n'
Cuts at: 0 901 1219 1411 1743 2604 2922
Size: 901 318 192 332 861 318
Fragments arranged by size:
901 861 332 318 318 192
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 588 1488 1581 2052 2922
Size: 588 900 93 471 870
Fragments arranged by size:
900 870 588 471 93
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 502 960 1077 1363 1483 2018 2677
Size: 244 258 458 117 286 120 535 659
Cuts at: 2677 2922
Size: 245
Fragments arranged by size:
659 535 458 286 258 245 244 120
117
Bpu10I CC'TnA_GC
Cuts at: 0 619 1885 2623 2922
Size: 619 1266 738 299
Fragments arranged by size:
1266 738 619 299
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 315 1065 2922
Size: 315 750 1857
Fragments arranged by size:
1857 750 315
BsaI GGTCTCn'nnnn_
Cuts at: 0 190 1111 2922
Size: 190 921 1811
Fragments arranged by size:
1811 921 190
BsaHI Gr'CG_yC
Cuts at: 0 2115 2922
Size: 2115 807
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 371 401 1336 1366 1991 2021 2922
Size: 371 30 935 30 625 30 901
Fragments arranged by size:
935 901 625 371 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 133 663 1009 1023 1411 1414 2922
Size: 133 530 346 14 388 3 1508
Fragments arranged by size:
1508 530 388 346 133 14 3
BseYI C'CCAG_C
Cuts at: 0 312 1061 1129 2045 2390 2550 2885 2922
Size: 312 749 68 916 345 160 335 37
Fragments arranged by size:
916 749 345 335 312 160 68 37
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 38 2309 2713 2806 2922
Size: 38 2271 404 93 116
Fragments arranged by size:
2271 404 116 93 38
BsiEI CG_ry'CG
Cuts at: 0 2284 2353 2869 2922
Size: 2284 69 516 53
Fragments arranged by size:
2284 516 69 53
BsiHKAI G_wGCw'C
Cuts at: 0 91 247 1567 1582 2922
Size: 91 156 1320 15 1340
Fragments arranged by size:
1340 1320 156 91 15
BsmI GAATG_Cn'
Cuts at: 0 144 2922
Size: 144 2778
BsmBI CGTCTCn'nnnn_
Cuts at: 0 955 2922
Size: 955 1967
Bsp1286I G_dGCh'C
Cuts at: 0 91 247 501 845 1001 1349 1567 1582
Size: 91 156 254 344 156 348 218 15
Cuts at: 1582 1976 2832 2900 2921 2922
Size: 394 856 68 21 1
Fragments arranged by size:
856 394 348 344 254 218 156 156
91 68 21 15 1
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 10 765 2774 2922
Size: 10 755 2009 148
Fragments arranged by size:
2009 755 148 10
BsrBI CCG'CTC
Cuts at: 0 2672 2826 2865 2922
Size: 2672 154 39 57
Fragments arranged by size:
2672 154 57 39
BsrDI GCAATG_nn'
Cuts at: 0 1302 2922
Size: 1302 1620
BsrFI r'CCGG_y
Cuts at: 0 2585 2874 2922
Size: 2585 289 48
Fragments arranged by size:
2585 289 48
BssSI C'ACGA_G
Cuts at: 0 181 494 2860 2922
Size: 181 313 2366 62
Fragments arranged by size:
2366 313 181 62
BstBI TT'CG_AA
Cuts at: 0 60 2922
Size: 60 2862
BstXI CCAn_nnnn'nTGG
Cuts at: 0 984 1749 2205 2922
Size: 984 765 456 717
Fragments arranged by size:
984 765 717 456
BstYI r'GATC_y
Cuts at: 0 332 2570 2922
Size: 332 2238 352
Fragments arranged by size:
2238 352 332
Bsu36I CC'TnA_GG
Cuts at: 0 846 1148 1517 2037 2922
Size: 846 302 369 520 885
Fragments arranged by size:
885 846 520 369 302
BtgI C'CryG_G
Cuts at: 0 200 1015 1607 1700 2104 2922
Size: 200 815 592 93 404 818
Fragments arranged by size:
818 815 592 404 200 93
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1421 2922
Size: 1421 1501
BtsI GCAGTG_nn'
Cuts at: 0 1336 2085 2297 2922
Size: 1336 749 212 625
Fragments arranged by size:
1336 749 625 212
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2061 2096 2922
Size: 2061 35 826
Fragments arranged by size:
2061 826 35
DraIII CAC_nnn'GTG
Cuts at: 0 1362 2922
Size: 1362 1560
DrdI GACnn_nn'nnGTC
Cuts at: 0 907 2275 2536 2922
Size: 907 1368 261 386
Fragments arranged by size:
1368 907 386 261
EaeI y'GGCC_r
Cuts at: 0 2 647 665 935 1223 2243 2281 2350
Size: 2 645 18 270 288 1020 38 69
Cuts at: 2350 2648 2866 2922
Size: 298 218 56
Fragments arranged by size:
1020 645 298 288 270 218 69 56
38 18 2
EagI C'GGCC_G
Cuts at: 0 2281 2350 2866 2922
Size: 2281 69 516 56
Fragments arranged by size:
2281 516 69 56
EarI CTCTTCn'nnn_
Cuts at: 0 1201 1462 1723 2922
Size: 1201 261 261 1199
Fragments arranged by size:
1201 1199 261 261
EcoICRI GAG'CTC
Cuts at: 0 89 1565 1580 2922
Size: 89 1476 15 1342
Fragments arranged by size:
1476 1342 89 15
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 149 244 453 502 750 763 960 1077
Size: 149 95 209 49 248 13 197 117
Cuts at: 1077 1363 1483 1952 2018 2677 2922
Size: 286 120 469 66 659 245
Fragments arranged by size:
659 469 286 248 245 209 197 149
120 117 95 66 49 13
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1768 2482 2626 2710 2922
Size: 1768 714 144 84 212
Fragments arranged by size:
1768 714 212 144 84
EcoO109I rG'GnC_Cy
Cuts at: 0 435 804 841 842 1947 1973 2234 2897
Size: 435 369 37 1 1105 26 261 663
Cuts at: 2897 2922
Size: 25
Fragments arranged by size:
1105 663 435 369 261 37 26 25
1
EcoRI G'AATT_C
Cuts at: 0 100 2922
Size: 100 2822
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2481 2736 2922
Size: 2481 255 186
Fragments arranged by size:
2481 255 186
FseI GG_CCGG'CC
Cuts at: 0 2589 2922
Size: 2589 333
FspI TGC'GCA
Cuts at: 0 1308 2473 2710 2770 2922
Size: 1308 1165 237 60 152
Fragments arranged by size:
1308 1165 237 152 60
HaeII r_GCGC'y
Cuts at: 0 369 1539 1811 2118 2881 2922
Size: 369 1170 272 307 763 41
Fragments arranged by size:
1170 763 369 307 272 41
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1399 1431 2922
Size: 1399 32 1491
Fragments arranged by size:
1491 1399 32
KasI G'GCGC_C
Cuts at: 0 2114 2922
Size: 2114 808
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 965 1208 2450 2828 2922
Size: 965 243 1242 378 94
Fragments arranged by size:
1242 965 378 243 94
MscI TGG'CCA
Cuts at: 0 4 667 937 1225 2922
Size: 4 663 270 288 1697
Fragments arranged by size:
1697 663 288 270 4
MslI CAynn'nnrTG
Cuts at: 0 154 1747 2782 2922
Size: 154 1593 1035 140
Fragments arranged by size:
1593 1035 154 140
MspA1I CmG'CkG
Cuts at: 0 1017 2045 2106 2370 2448 2871 2885 2922
Size: 1017 1028 61 264 78 423 14 37
Fragments arranged by size:
1028 1017 423 264 78 61 37 14
NaeI GCC'GGC
Cuts at: 0 2587 2876 2922
Size: 2587 289 46
Fragments arranged by size:
2587 289 46
NarI GG'CG_CC
Cuts at: 0 2115 2922
Size: 2115 807
NcoI C'CATG_G
Cuts at: 0 200 2922
Size: 200 2722
NgoMIV G'CCGG_C
Cuts at: 0 2585 2874 2922
Size: 2585 289 48
Fragments arranged by size:
2585 289 48
NotI GC'GGCC_GC
Cuts at: 0 2866 2922
Size: 2866 56
NspI r_CATG'y
Cuts at: 0 385 2361 2922
Size: 385 1976 561
Fragments arranged by size:
1976 561 385
PasI CC'CwG_GG
Cuts at: 0 318 516 2198 2922
Size: 318 198 1682 724
Fragments arranged by size:
1682 724 318 198
PciI A'CATG_T
Cuts at: 0 381 2922
Size: 381 2541
PflMI CCAn_nnn'nTGG
Cuts at: 0 1637 1733 1950 2922
Size: 1637 96 217 972
Fragments arranged by size:
1637 972 217 96
PfoI T'CCnGG_A
Cuts at: 0 260 416 1460 2922
Size: 260 156 1044 1462
Fragments arranged by size:
1462 1044 260 156
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1336 1368 2922
Size: 1336 32 1554
Fragments arranged by size:
1554 1336 32
PpuMI rG'GwC_Cy
Cuts at: 0 435 1947 2234 2922
Size: 435 1512 287 688
Fragments arranged by size:
1512 688 435 287
PshAI GACnn'nnGTC
Cuts at: 0 382 1876 2922
Size: 382 1494 1046
Fragments arranged by size:
1494 1046 382
PspOMI G'GGCC_C
Cuts at: 0 841 1972 2828 2896 2922
Size: 841 1131 856 68 26
Fragments arranged by size:
1131 856 841 68 26
PspXI vC'TCGA_Gb
Cuts at: 0 1330 1524 2922
Size: 1330 194 1398
Fragments arranged by size:
1398 1330 194
PstI C_TGCA'G
Cuts at: 0 1282 1827 2922
Size: 1282 545 1095
Fragments arranged by size:
1282 1095 545
PvuII CAG'CTG
Cuts at: 0 2885 2922
Size: 2885 37
SacI G_AGCT'C
Cuts at: 0 91 1567 1582 2922
Size: 91 1476 15 1340
Fragments arranged by size:
1476 1340 91 15
SacII CC_GC'GG
Cuts at: 0 1018 2107 2922
Size: 1018 1089 815
Fragments arranged by size:
1089 1018 815
SanDI GG'GwC_CC
Cuts at: 0 435 2922
Size: 435 2487
SexAI A'CCwGG_T
Cuts at: 0 722 1358 2922
Size: 722 636 1564
Fragments arranged by size:
1564 722 636
SfcI C'TryA_G
Cuts at: 0 597 729 831 1278 1290 1823 2541 2922
Size: 597 132 102 447 12 533 718 381
Fragments arranged by size:
718 597 533 447 381 132 102 12
SfoI GGC'GCC
Cuts at: 0 2116 2922
Size: 2116 806
SmaI CCC'GGG
Cuts at: 0 35 1477 1849 2922
Size: 35 1442 372 1073
Fragments arranged by size:
1442 1073 372 35
SmlI C'TyrA_G
Cuts at: 0 294 1080 1330 1524 2922
Size: 294 786 250 194 1398
Fragments arranged by size:
1398 786 294 250 194
StuI AGG'CCT
Cuts at: 0 1522 2922
Size: 1522 1400
StyI C'CwwG_G
Cuts at: 0 5 200 2273 2922
Size: 5 195 2073 649
Fragments arranged by size:
2073 649 195 5
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 910 1075 1459 1600 2922
Size: 910 165 384 141 1322
Fragments arranged by size:
1322 910 384 165 141
TatI w'GTAC_w
Cuts at: 0 1667 2922
Size: 1667 1255
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 371 403 1991 2023 2922
Size: 371 32 1588 32 899
Fragments arranged by size:
1588 899 371 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 637 2922
Size: 637 2285
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 120 180 207 1356 1749 2922
Size: 120 60 27 1149 393 1173
Fragments arranged by size:
1173 1149 393 120 60 27
XhoI C'TCGA_G
Cuts at: 0 1330 1524 2922
Size: 1330 194 1398
Fragments arranged by size:
1398 1330 194
XmaI C'CCGG_G
Cuts at: 0 33 1475 1847 2922
Size: 33 1442 372 1075
Fragments arranged by size:
1442 1075 372 33
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AleI AlfI AloI
AlwNI ApaI ApaLI ApoI AvaI BanI BanII BbeI
BbsI BbvCI BcgI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspMI BsrBI BsrDI BsrFI BssSI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI CspCI DraIII DrdI EaeI
EagI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FseI FspI HaeII Hin4I KasI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NotI NspI PasI
PciI PflMI PfoI PpiI PpuMI PshAI PspOMI PspXI
PstI PvuII SacI SacII SanDI SexAI SfcI SfoI
SmaI SmlI StuI StyI TaqII TatI TstI Tth111I
XcmI XhoI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AhdI AscI AseI
AsiSI AvrII BaeI BamHI BciVI BclI BfrBI BmtI
BsaAI BsaBI BsaWI BsiWI BspEI BspHI BsrGI BssHII
BstAPI BstEII BstZ17I ClaI DraI EciI EcoRV FspAI
HincII HindIII HpaI KpnI MfeI MluI NdeI NheI
NruI NsiI PacI PmeI PmlI PsiI PsrI PvuI
RsrII SalI SapI SbfI ScaI SfiI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI SwaI XbaI XmnI
ZraI