Restriction map for
pFN21AE3334
(Linear) (Six-base) MAPSORT of: KIEE3334.seq Check: 3809 from: 1 to: 2904
KIEE3334.orf
With 182 enzymes: SgfI *
July 3, 2012 17:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 268 1425 2904
Size: 268 1157 1479
Fragments arranged by size:
1479 1157 268
AccI GT'mk_AC
Cuts at: 0 37 2904
Size: 37 2867
Acc65I G'GTAC_C
Cuts at: 0 1495 2904
Size: 1495 1409
AclI AA'CG_TT
Cuts at: 0 1788 2904
Size: 1788 1116
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 568 832 2404 2716 2749 2904
Size: 568 264 1572 312 33 155
Fragments arranged by size:
1572 568 312 264 155 33
AfeI AGC'GCT
Cuts at: 0 2356 2904
Size: 2356 548
AhdI GACnn_n'nnGTC
Cuts at: 0 660 2904
Size: 660 2244
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1907 2904
Size: 1907 997
AlwNI CAG_nnn'CTG
Cuts at: 0 864 1219 1469 2646 2694 2727 2904
Size: 864 355 250 1177 48 33 177
Fragments arranged by size:
1177 864 355 250 177 48 33
ApoI r'AATT_y
Cuts at: 0 1262 1594 2904
Size: 1262 332 1310
Fragments arranged by size:
1310 1262 332
AvaI C'yCGr_G
Cuts at: 0 499 601 1072 2904
Size: 499 102 471 1832
Fragments arranged by size:
1832 499 471 102
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1479 1512 2904
Size: 1479 33 1392
Fragments arranged by size:
1479 1392 33
BamHI G'GATC_C
Cuts at: 0 1922 2904
Size: 1922 982
BanI G'GyrC_C
Cuts at: 0 133 1098 1495 1685 2261 2904
Size: 133 965 397 190 576 643
Fragments arranged by size:
965 643 576 397 190 133
BanII G_rGCy'C
Cuts at: 0 395 719 1133 1540 2204 2904
Size: 395 324 414 407 664 700
Fragments arranged by size:
700 664 414 407 395 324
BbeI G_GCGC'C
Cuts at: 0 137 1689 2904
Size: 137 1552 1215
Fragments arranged by size:
1552 1215 137
BbsI GAAGACnn'nnnn_
Cuts at: 0 556 962 2904
Size: 556 406 1942
Fragments arranged by size:
1942 556 406
BbvCI CC'TCA_GC
Cuts at: 0 923 1309 2904
Size: 923 386 1595
Fragments arranged by size:
1595 923 386
BfrBI ATG'CAT
Cuts at: 0 1782 2904
Size: 1782 1122
BglII A'GATC_T
Cuts at: 0 468 1327 2159 2904
Size: 468 859 832 745
Fragments arranged by size:
859 832 745 468
BlpI GC'TnA_GC
Cuts at: 0 1292 2501 2821 2904
Size: 1292 1209 320 83
Fragments arranged by size:
1292 1209 320 83
Bme1580I G_kGCm'C
Cuts at: 0 1226 2842 2904
Size: 1226 1616 62
Fragments arranged by size:
1616 1226 62
BmgBI CAC'GTC
Cuts at: 0 75 2904
Size: 75 2829
BmrI ACTGGGnnnn_n'
Cuts at: 0 842 1174 2904
Size: 842 332 1730
Fragments arranged by size:
1730 842 332
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1554 2904
Size: 1554 1350
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 388 886 1030 1390 1415 1429 1699
Size: 244 144 498 144 360 25 14 270
Cuts at: 1699 1792 1867 1951 2137 2287 2904
Size: 93 75 84 186 150 617
Fragments arranged by size:
617 498 360 270 244 186 150 144
144 93 84 75 25 14
Bpu10I CC'TnA_GC
Cuts at: 0 923 1309 2904
Size: 923 386 1595
Fragments arranged by size:
1595 923 386
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1564 2904
Size: 1564 1340
BsaI GGTCTCn'nnnn_
Cuts at: 0 613 1366 2204 2904
Size: 613 753 838 700
Fragments arranged by size:
838 753 700 613
BsaBI GATnn'nnATC
Cuts at: 0 247 2904
Size: 247 2657
BsaHI Gr'CG_yC
Cuts at: 0 134 356 986 1686 2904
Size: 134 222 630 700 1218
Fragments arranged by size:
1218 700 630 222 134
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2164 2194 2821 2851 2904
Size: 2164 30 627 30 53
Fragments arranged by size:
2164 627 53 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 229 232 994 1020 1090 1681 1924 2068
Size: 229 3 762 26 70 591 243 144
Cuts at: 2068 2445 2554 2842 2904
Size: 377 109 288 62
Fragments arranged by size:
762 591 377 288 243 229 144 109
70 62 26 3
BseYI C'CCAG_C
Cuts at: 0 123 1102 1314 1742 2531 2904
Size: 123 979 212 428 789 373
Fragments arranged by size:
979 789 428 373 212 123
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 427 1455 2904
Size: 427 1028 1449
Fragments arranged by size:
1449 1028 427
BsiEI CG_ry'CG
Cuts at: 0 963 2755 2904
Size: 963 1792 149
Fragments arranged by size:
1792 963 149
BsiHKAI G_wGCw'C
Cuts at: 0 74 719 2060 2204 2246 2681 2904
Size: 74 645 1341 144 42 435 223
Fragments arranged by size:
1341 645 435 223 144 74 42
BsmBI CGTCTCn'nnnn_
Cuts at: 0 163 2904
Size: 163 2741
Bsp1286I G_dGCh'C
Cuts at: 0 74 395 719 1133 1226 1540 2060 2204
Size: 74 321 324 414 93 314 520 144
Cuts at: 2204 2246 2681 2842 2904
Size: 42 435 161 62
Fragments arranged by size:
520 435 414 324 321 314 161 144
93 74 62 42
BspHI T'CATG_A
Cuts at: 0 323 2186 2904
Size: 323 1863 718
Fragments arranged by size:
1863 718 323
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 268 1425 2904
Size: 268 1157 1479
Fragments arranged by size:
1479 1157 268
BsrBI CCG'CTC
Cuts at: 0 2340 2562 2904
Size: 2340 222 342
Fragments arranged by size:
2340 342 222
BsrFI r'CCGG_y
Cuts at: 0 1349 2904
Size: 1349 1555
BsrGI T'GTAC_A
Cuts at: 0 1565 2459 2904
Size: 1565 894 445
Fragments arranged by size:
1565 894 445
BssSI C'ACGA_G
Cuts at: 0 712 2311 2584 2904
Size: 712 1599 273 320
Fragments arranged by size:
1599 712 320 273
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1469 1764 2250 2316 2646 2904
Size: 1469 295 486 66 330 258
Fragments arranged by size:
1469 486 330 295 258 66
BstXI CCAn_nnnn'nTGG
Cuts at: 0 366 1095 1147 2904
Size: 366 729 52 1757
Fragments arranged by size:
1757 729 366 52
BstYI r'GATC_y
Cuts at: 0 248 468 1156 1327 1922 2159 2423 2778
Size: 248 220 688 171 595 237 264 355
Cuts at: 2778 2904
Size: 126
Fragments arranged by size:
688 595 355 264 248 237 220 171
126
BtgI C'CryG_G
Cuts at: 0 350 989 2904
Size: 350 639 1915
Fragments arranged by size:
1915 639 350
BtsI GCAGTG_nn'
Cuts at: 0 1220 2620 2904
Size: 1220 1400 284
Fragments arranged by size:
1400 1220 284
EaeI y'GGCC_r
Cuts at: 0 188 254 960 1394 2123 2453 2752 2904
Size: 188 66 706 434 729 330 299 152
Fragments arranged by size:
729 706 434 330 299 188 152 66
EagI C'GGCC_G
Cuts at: 0 960 2752 2904
Size: 960 1792 152
Fragments arranged by size:
1792 960 152
EarI CTCTTCn'nnn_
Cuts at: 0 43 941 971 1718 2125 2234 2461 2539
Size: 43 898 30 747 407 109 227 78
Cuts at: 2539 2768 2904
Size: 229 136
Fragments arranged by size:
898 747 407 229 227 136 109 78
43 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 7 67 1034 1915 1935 2848 2904
Size: 7 60 967 881 20 913 56
Fragments arranged by size:
967 913 881 60 56 20 7
EcoICRI GAG'CTC
Cuts at: 0 717 2202 2904
Size: 717 1485 702
Fragments arranged by size:
1485 717 702
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 388 568 832 886 1030 1390 1415
Size: 244 144 180 264 54 144 360 25
Cuts at: 1415 1429 1699 1792 1867 1951 2137 2287 2404
Size: 14 270 93 75 84 186 150 117
Cuts at: 2404 2716 2749 2904
Size: 312 33 155
Fragments arranged by size:
360 312 270 264 244 186 180 155
150 144 144 117 93 84 75 54
33 25 14
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1933 2904
Size: 1933 971
EcoO109I rG'GnC_Cy
Cuts at: 0 732 1305 1435 1554 2112 2700 2904
Size: 732 573 130 119 558 588 204
Fragments arranged by size:
732 588 573 558 204 130 119
EcoRI G'AATT_C
Cuts at: 0 1262 2904
Size: 1262 1642
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1839 2904
Size: 1839 1065
FspI TGC'GCA
Cuts at: 0 2323 2904
Size: 2323 581
HaeII r_GCGC'y
Cuts at: 0 137 1367 1689 2358 2904
Size: 137 1230 322 669 546
Fragments arranged by size:
1230 669 546 322 137
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 646 678 985 1017 1524 1556 2904
Size: 646 32 307 32 507 32 1348
Fragments arranged by size:
1348 646 507 307 32 32 32
HindIII A'AGCT_T
Cuts at: 0 2746 2904
Size: 2746 158
KasI G'GCGC_C
Cuts at: 0 133 1685 2904
Size: 133 1552 1219
Fragments arranged by size:
1552 1219 133
KpnI G_GTAC'C
Cuts at: 0 1499 2904
Size: 1499 1405
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 734 2537 2904
Size: 734 1803 367
Fragments arranged by size:
1803 734 367
MscI TGG'CCA
Cuts at: 0 256 1396 2125 2455 2904
Size: 256 1140 729 330 449
Fragments arranged by size:
1140 729 449 330 256
MslI CAynn'nnrTG
Cuts at: 0 2455 2904
Size: 2455 449
MspA1I CmG'CkG
Cuts at: 0 861 1095 1746 1770 2025 2250 2268 2307
Size: 861 234 651 24 255 225 18 39
Cuts at: 2307 2382 2500 2646 2904
Size: 75 118 146 258
Fragments arranged by size:
861 651 258 255 234 225 146 118
75 39 24 18
NaeI GCC'GGC
Cuts at: 0 1351 2904
Size: 1351 1553
NarI GG'CG_CC
Cuts at: 0 134 1686 2904
Size: 134 1552 1218
Fragments arranged by size:
1552 1218 134
NgoMIV G'CCGG_C
Cuts at: 0 1349 2904
Size: 1349 1555
NsiI A_TGCA'T
Cuts at: 0 1784 2904
Size: 1784 1120
NspI r_CATG'y
Cuts at: 0 2607 2904
Size: 2607 297
PasI CC'CwG_GG
Cuts at: 0 666 2904
Size: 666 2238
PflMI CCAn_nnn'nTGG
Cuts at: 0 2670 2904
Size: 2670 234
PpuMI rG'GwC_Cy
Cuts at: 0 732 1305 1554 2112 2904
Size: 732 573 249 558 792
Fragments arranged by size:
792 732 573 558 249
PshAI GACnn'nnGTC
Cuts at: 0 1245 2904
Size: 1245 1659
PspXI vC'TCGA_Gb
Cuts at: 0 499 2904
Size: 499 2405
PstI C_TGCA'G
Cuts at: 0 1138 1462 1769 2255 2570 2642 2651 2904
Size: 1138 324 307 486 315 72 9 253
Fragments arranged by size:
1138 486 324 315 307 253 72 9
PvuII CAG'CTG
Cuts at: 0 861 1746 1770 2250 2268 2382 2500 2646
Size: 861 885 24 480 18 114 118 146
Cuts at: 2646 2904
Size: 258
Fragments arranged by size:
885 861 480 258 146 118 114 24
18
SacI G_AGCT'C
Cuts at: 0 719 2204 2904
Size: 719 1485 700
Fragments arranged by size:
1485 719 700
SapI GCTCTTCn'nnn_
Cuts at: 0 1718 2234 2461 2904
Size: 1718 516 227 443
Fragments arranged by size:
1718 516 443 227
ScaI AGT'ACT
Cuts at: 0 2296 2904
Size: 2296 608
SexAI A'CCwGG_T
Cuts at: 0 1951 2904
Size: 1951 953
SfcI C'TryA_G
Cuts at: 0 556 791 1134 1458 1765 2251 2566 2638
Size: 556 235 343 324 307 486 315 72
Cuts at: 2638 2647 2883 2904
Size: 9 236 21
Fragments arranged by size:
556 486 343 324 315 307 236 235
72 21 9
SfoI GGC'GCC
Cuts at: 0 135 1687 2904
Size: 135 1552 1217
Fragments arranged by size:
1552 1217 135
SmlI C'TyrA_G
Cuts at: 0 499 1072 1579 2904
Size: 499 573 507 1325
Fragments arranged by size:
1325 573 507 499
StuI AGG'CCT
Cuts at: 0 383 2904
Size: 383 2521
StyI C'CwwG_G
Cuts at: 0 929 1376 2904
Size: 929 447 1528
Fragments arranged by size:
1528 929 447
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1116 1369 2904
Size: 1116 253 1535
Fragments arranged by size:
1535 1116 253
TatI w'GTAC_w
Cuts at: 0 1565 2294 2459 2904
Size: 1565 729 165 445
Fragments arranged by size:
1565 729 445 165
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2164 2196 2821 2853 2904
Size: 2164 32 625 32 51
Fragments arranged by size:
2164 625 51 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1404 2904
Size: 1404 1500
XhoI C'TCGA_G
Cuts at: 0 499 1072 2904
Size: 499 573 1832
Fragments arranged by size:
1832 573 499
XmnI GAAnn'nnTTC
Cuts at: 0 1819 2904
Size: 1819 1085
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AhdI AlfI
AlwNI ApoI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BfrBI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrFI BsrGI BssSI BstAPI BstXI BstYI
BtgI BtsI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HindIII
KasI KpnI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NsiI NspI PasI PflMI PpuMI PshAI PspXI
PstI PvuII SacI SapI ScaI SexAI SfcI SfoI
SmlI StuI StyI TaqII TatI TstI XcmI XhoI
XmnI
Enzymes that do not cut:
AatII AflII AflIII AgeI AleI AloI ApaI ApaLI
AscI AseI AsiSI AvrII BcgI BciVI BclI BglI
BmtI BsaAI BsaWI BsiWI BsmI BspEI BsrDI BssHII
BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DraIII DrdI EcoRV FseI FspAI HincII HpaI MfeI
MluI NcoI NdeI NheI NotI NruI PacI PciI
PfoI PmeI PmlI PpiI PsiI PspOMI PsrI PvuI
RsrII SacII SalI SanDI SbfI SfiI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI SwaI Tth111I
XbaI XmaI ZraI