Restriction map for
pFN21AE3333
(Linear) (Six-base) MAPSORT of: KIEE3333.seq Check: 5334 from: 1 to: 2901
KIEE3333.orf
With 182 enzymes: SgfI *
July 3, 2012 17:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 465 1006 2901
Size: 465 541 1895
Fragments arranged by size:
1895 541 465
AccI GT'mk_AC
Cuts at: 0 817 1962 2901
Size: 817 1145 939
Fragments arranged by size:
1145 939 817
AclI AA'CG_TT
Cuts at: 0 2766 2901
Size: 2766 135
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 126 241 1075 1360 1654 1883 2901
Size: 126 115 834 285 294 229 1018
Fragments arranged by size:
1018 834 294 285 229 126 115
AfeI AGC'GCT
Cuts at: 0 1559 2901
Size: 1559 1342
AgeI A'CCGG_T
Cuts at: 0 2560 2901
Size: 2560 341
AhdI GACnn_n'nnGTC
Cuts at: 0 397 869 2901
Size: 397 472 2032
Fragments arranged by size:
2032 472 397
AleI CACnn'nnGTG
Cuts at: 0 2561 2901
Size: 2561 340
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 427 2901
Size: 427 2474
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 781 813 2901
Size: 781 32 2088
Fragments arranged by size:
2088 781 32
ApaLI G'TGCA_C
Cuts at: 0 412 1492 2901
Size: 412 1080 1409
Fragments arranged by size:
1409 1080 412
ApoI r'AATT_y
Cuts at: 0 1432 2448 2884 2901
Size: 1432 1016 436 17
Fragments arranged by size:
1432 1016 436 17
AseI AT'TA_AT
Cuts at: 0 2056 2901
Size: 2056 845
AvaI C'yCGr_G
Cuts at: 0 292 503 1754 2901
Size: 292 211 1251 1147
Fragments arranged by size:
1251 1147 292 211
BamHI G'GATC_C
Cuts at: 0 1842 2010 2901
Size: 1842 168 891
Fragments arranged by size:
1842 891 168
BanI G'GyrC_C
Cuts at: 0 1459 2455 2901
Size: 1459 996 446
Fragments arranged by size:
1459 996 446
BanII G_rGCy'C
Cuts at: 0 98 1975 2530 2901
Size: 98 1877 555 371
Fragments arranged by size:
1877 555 371 98
BbsI GAAGACnn'nnnn_
Cuts at: 0 390 1898 2139 2901
Size: 390 1508 241 762
Fragments arranged by size:
1508 762 390 241
BciVI GTATCCnnnnn_n'
Cuts at: 0 907 2901
Size: 907 1994
BclI T'GATC_A
Cuts at: 0 2369 2901
Size: 2369 532
BfrBI ATG'CAT
Cuts at: 0 2123 2901
Size: 2123 778
Bme1580I G_kGCm'C
Cuts at: 0 315 416 1464 1496 2460 2754 2901
Size: 315 101 1048 32 964 294 147
Fragments arranged by size:
1048 964 315 294 147 101 32
BmrI ACTGGGnnnn_n'
Cuts at: 0 1506 2654 2748 2901
Size: 1506 1148 94 153
Fragments arranged by size:
1506 1148 153 94
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 444 2901
Size: 444 2457
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 1183 1940 2750 2901
Size: 58 1125 757 810 151
Fragments arranged by size:
1125 810 757 151 58
Bpu10I CC'TnA_GC
Cuts at: 0 1361 2901
Size: 1361 1540
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 274 2901
Size: 274 2627
BsaI GGTCTCn'nnnn_
Cuts at: 0 118 475 801 1861 2229 2901
Size: 118 357 326 1060 368 672
Fragments arranged by size:
1060 672 368 357 326 118
BsaAI yAC'GTr
Cuts at: 0 699 1704 2901
Size: 699 1005 1197
Fragments arranged by size:
1197 1005 699
BsaWI w'CCGG_w
Cuts at: 0 1631 2560 2630 2901
Size: 1631 929 70 271
Fragments arranged by size:
1631 929 271 70
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 781 811 2268 2298 2901
Size: 781 30 1457 30 603
Fragments arranged by size:
1457 781 603 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1399 1402 1933 2433 2901
Size: 1399 3 531 500 468
Fragments arranged by size:
1399 531 500 468 3
BseYI C'CCAG_C
Cuts at: 0 667 1774 1853 2098 2901
Size: 667 1107 79 245 803
Fragments arranged by size:
1107 803 667 245 79
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 830 980 2901
Size: 830 150 1921
Fragments arranged by size:
1921 830 150
BsiHKAI G_wGCw'C
Cuts at: 0 416 1296 1496 2281 2530 2901
Size: 416 880 200 785 249 371
Fragments arranged by size:
880 785 416 371 249 200
BsmI GAATG_Cn'
Cuts at: 0 588 876 2125 2901
Size: 588 288 1249 776
Fragments arranged by size:
1249 776 588 288
Bsp1286I G_dGCh'C
Cuts at: 0 98 315 416 1296 1464 1496 1975 2281
Size: 98 217 101 880 168 32 479 306
Cuts at: 2281 2460 2530 2754 2901
Size: 179 70 224 147
Fragments arranged by size:
880 479 306 224 217 179 168 147
101 98 70 32
BspEI T'CCGG_A
Cuts at: 0 1631 2901
Size: 1631 1270
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 387 465 1006 2901
Size: 387 78 541 1895
Fragments arranged by size:
1895 541 387 78
BsrFI r'CCGG_y
Cuts at: 0 212 2560 2901
Size: 212 2348 341
Fragments arranged by size:
2348 341 212
BstAPI GCAn_nnn'nTGC
Cuts at: 0 958 1291 2901
Size: 958 333 1610
Fragments arranged by size:
1610 958 333
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1120 2901
Size: 1120 1781
BstYI r'GATC_y
Cuts at: 0 1842 2010 2901
Size: 1842 168 891
Fragments arranged by size:
1842 891 168
BtgI C'CryG_G
Cuts at: 0 859 1455 2901
Size: 859 596 1446
Fragments arranged by size:
1446 859 596
BtsI GCAGTG_nn'
Cuts at: 0 328 818 994 1908 2901
Size: 328 490 176 914 993
Fragments arranged by size:
993 914 490 328 176
DraI TTT'AAA
Cuts at: 0 2409 2901
Size: 2409 492
DrdI GACnn_nn'nnGTC
Cuts at: 0 1958 2901
Size: 1958 943
EaeI y'GGCC_r
Cuts at: 0 701 1227 1698 2901
Size: 701 526 471 1203
Fragments arranged by size:
1203 701 526 471
EarI CTCTTCn'nnn_
Cuts at: 0 1336 1573 1669 1859 2227 2901
Size: 1336 237 96 190 368 674
Fragments arranged by size:
1336 674 368 237 190 96
EcoICRI GAG'CTC
Cuts at: 0 2528 2901
Size: 2528 373
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 126 241 1075 1183 1360 1654 1883
Size: 58 68 115 834 108 177 294 229
Cuts at: 1883 1940 2750 2901
Size: 57 810 151
Fragments arranged by size:
834 810 294 229 177 151 115 108
68 58 57
EcoNI CCTnn'n_nnAGG
Cuts at: 0 637 2901
Size: 637 2264
EcoO109I rG'GnC_Cy
Cuts at: 0 75 671 753 945 1951 2901
Size: 75 596 82 192 1006 950
Fragments arranged by size:
1006 950 596 192 82 75
EcoRV GAT'ATC
Cuts at: 0 1530 2901
Size: 1530 1371
HaeII r_GCGC'y
Cuts at: 0 1561 2627 2901
Size: 1561 1066 274
Fragments arranged by size:
1561 1066 274
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 73 105 383 415 717 749 855 887
Size: 73 32 278 32 302 32 106 32
Cuts at: 887 1737 1769 2268 2300 2517 2549 2901
Size: 850 32 499 32 217 32 352
Fragments arranged by size:
850 499 352 302 278 217 106 73
32 32 32 32 32 32 32
HindIII A'AGCT_T
Cuts at: 0 182 2265 2430 2901
Size: 182 2083 165 471
Fragments arranged by size:
2083 471 182 165
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 821 2038 2901
Size: 821 1217 863
Fragments arranged by size:
1217 863 821
MscI TGG'CCA
Cuts at: 0 703 1229 1700 2901
Size: 703 526 471 1201
Fragments arranged by size:
1201 703 526 471
MslI CAynn'nnrTG
Cuts at: 0 2561 2901
Size: 2561 340
MspA1I CmG'CkG
Cuts at: 0 546 2901
Size: 546 2355
NcoI C'CATG_G
Cuts at: 0 859 1455 2901
Size: 859 596 1446
Fragments arranged by size:
1446 859 596
NsiI A_TGCA'T
Cuts at: 0 2125 2901
Size: 2125 776
NspI r_CATG'y
Cuts at: 0 1236 2901
Size: 1236 1665
PasI CC'CwG_GG
Cuts at: 0 491 599 801 2901
Size: 491 108 202 2100
Fragments arranged by size:
2100 491 202 108
PmlI CAC'GTG
Cuts at: 0 699 1704 2901
Size: 699 1005 1197
Fragments arranged by size:
1197 1005 699
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 781 813 1927 1959 2901
Size: 781 32 1114 32 942
Fragments arranged by size:
1114 942 781 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 75 671 753 945 1951 2901
Size: 75 596 82 192 1006 950
Fragments arranged by size:
1006 950 596 192 82 75
PstI C_TGCA'G
Cuts at: 0 1287 1401 1970 2901
Size: 1287 114 569 931
Fragments arranged by size:
1287 931 569 114
PvuII CAG'CTG
Cuts at: 0 546 2901
Size: 546 2355
SacI G_AGCT'C
Cuts at: 0 2530 2901
Size: 2530 371
SanDI GG'GwC_CC
Cuts at: 0 671 2901
Size: 671 2230
SapI GCTCTTCn'nnn_
Cuts at: 0 1573 2901
Size: 1573 1328
SfcI C'TryA_G
Cuts at: 0 717 1283 1397 1598 1847 1966 2241 2490
Size: 717 566 114 201 249 119 275 249
Cuts at: 2490 2901
Size: 411
Fragments arranged by size:
717 566 411 275 249 249 201 119
114
SgrAI Cr'CCGG_yG
Cuts at: 0 2560 2901
Size: 2560 341
SmlI C'TyrA_G
Cuts at: 0 253 2901
Size: 253 2648
StuI AGG'CCT
Cuts at: 0 1096 2220 2901
Size: 1096 1124 681
Fragments arranged by size:
1124 1096 681
StyI C'CwwG_G
Cuts at: 0 239 859 1455 2901
Size: 239 620 596 1446
Fragments arranged by size:
1446 620 596 239
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 67 99 645 677 797 829 2901
Size: 67 32 546 32 120 32 2072
Fragments arranged by size:
2072 546 120 67 32 32 32
XmnI GAAnn'nnTTC
Cuts at: 0 1088 1559 1610 2147 2599 2901
Size: 1088 471 51 537 452 302
Fragments arranged by size:
1088 537 471 452 302 51
Enzymes that do cut:
AarI AccI AclI AcuI AfeI AgeI AhdI AleI
AlfI AloI ApaLI ApoI AseI AvaI BamHI BanI
BanII BbsI BciVI BclI BfrBI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrFI
BstAPI BstXI BstYI BtgI BtsI DraI DrdI EaeI
EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRV HaeII Hin4I
HindIII MmeI MscI MslI MspA1I NcoI NsiI NspI
PasI PmlI PpiI PpuMI PstI PvuII SacI SanDI
SapI SfcI SgrAI SmlI StuI StyI TstI XmnI
Enzymes that do not cut:
AatII Acc65I AflII AflIII AlwNI ApaI AscI AsiSI
AvrII BaeI BbeI BbvCI BcgI BglI BglII BlpI
BmgBI BmtI BsaBI BsaHI BsiEI BsiWI BsmBI BspHI
BsrBI BsrDI BsrGI BssHII BssSI BstBI BstEII BstZ17I
Bsu36I BtgZI ClaI CspCI DraIII EagI EciI EcoRI
FalI FseI FspI FspAI HincII HpaI KasI KpnI
MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI
NruI PacI PciI PflMI PfoI PmeI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SgfI SmaI SnaBI SpeI
SphI SrfI SspI SwaI TaqII TatI Tth111I XbaI
XcmI XhoI XmaI ZraI