Restriction map for
pFN21AE3329
(Linear) (Six-base) MAPSORT of: KIEE3329.seq Check: 5021 from: 1 to: 2811
KIEE3329.orf
With 182 enzymes: SgfI *
March 22, 2012 16:30 ..
AccI GT'mk_AC
Cuts at: 0 2584 2811
Size: 2584 227
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2003 2162 2267 2811
Size: 2003 159 105 544
Fragments arranged by size:
2003 544 159 105
AflIII A'CryG_T
Cuts at: 0 1332 2366 2660 2811
Size: 1332 1034 294 151
Fragments arranged by size:
1332 1034 294 151
AleI CACnn'nnGTG
Cuts at: 0 952 2811
Size: 952 1859
AlwNI CAG_nnn'CTG
Cuts at: 0 877 2811
Size: 877 1934
ApoI r'AATT_y
Cuts at: 0 899 1011 1555 1916 2002 2811
Size: 899 112 544 361 86 809
Fragments arranged by size:
899 809 544 361 112 86
AseI AT'TA_AT
Cuts at: 0 1664 2811
Size: 1664 1147
AvaI C'yCGr_G
Cuts at: 0 2373 2811
Size: 2373 438
BamHI G'GATC_C
Cuts at: 0 2623 2811
Size: 2623 188
BanI G'GyrC_C
Cuts at: 0 992 1308 1618 2811
Size: 992 316 310 1193
Fragments arranged by size:
1193 992 316 310
BanII G_rGCy'C
Cuts at: 0 305 871 2811
Size: 305 566 1940
Fragments arranged by size:
1940 566 305
BbeI G_GCGC'C
Cuts at: 0 996 2811
Size: 996 1815
BbsI GAAGACnn'nnnn_
Cuts at: 0 2150 2811
Size: 2150 661
BciVI GTATCCnnnnn_n'
Cuts at: 0 469 898 1031 2455 2811
Size: 469 429 133 1424 356
Fragments arranged by size:
1424 469 429 356 133
BfrBI ATG'CAT
Cuts at: 0 1372 2811
Size: 1372 1439
BglI GCCn_nnn'nGGC
Cuts at: 0 309 2811
Size: 309 2502
BglII A'GATC_T
Cuts at: 0 849 2811
Size: 849 1962
BlpI GC'TnA_GC
Cuts at: 0 1499 2811
Size: 1499 1312
BmgBI CAC'GTC
Cuts at: 0 206 1335 2229 2811
Size: 206 1129 894 582
Fragments arranged by size:
1129 894 582 206
BmrI ACTGGGnnnn_n'
Cuts at: 0 1011 2323 2811
Size: 1011 1312 488
Fragments arranged by size:
1312 1011 488
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2271 2811
Size: 2271 540
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 449 784 2053 2811
Size: 449 335 1269 758
Fragments arranged by size:
1269 758 449 335
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 129 2241 2811
Size: 129 2112 570
Fragments arranged by size:
2112 570 129
BsaI GGTCTCn'nnnn_
Cuts at: 0 2199 2811
Size: 2199 612
BsaHI Gr'CG_yC
Cuts at: 0 993 2765 2811
Size: 993 1772 46
Fragments arranged by size:
1772 993 46
BsaWI w'CCGG_w
Cuts at: 0 890 2811
Size: 890 1921
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2505 2535 2811
Size: 2505 30 276
Fragments arranged by size:
2505 276 30
BseYI C'CCAG_C
Cuts at: 0 857 1164 1541 2811
Size: 857 307 377 1270
Fragments arranged by size:
1270 857 377 307
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 1573 1762 2811
Size: 214 1359 189 1049
Fragments arranged by size:
1359 1049 214 189
BsiEI CG_ry'CG
Cuts at: 0 10 2811
Size: 10 2801
BsmI GAATG_Cn'
Cuts at: 0 1374 2811
Size: 1374 1437
BsmBI CGTCTCn'nnnn_
Cuts at: 0 212 2756 2811
Size: 212 2544 55
Fragments arranged by size:
2544 212 55
Bsp1286I G_dGCh'C
Cuts at: 0 305 871 2811
Size: 305 566 1940
Fragments arranged by size:
1940 566 305
BspEI T'CCGG_A
Cuts at: 0 890 2811
Size: 890 1921
BspHI T'CATG_A
Cuts at: 0 1694 2171 2811
Size: 1694 477 640
Fragments arranged by size:
1694 640 477
BsrBI CCG'CTC
Cuts at: 0 36 2811
Size: 36 2775
BsrDI GCAATG_nn'
Cuts at: 0 1250 1552 1781 2811
Size: 1250 302 229 1030
Fragments arranged by size:
1250 1030 302 229
BsrFI r'CCGG_y
Cuts at: 0 5 2811
Size: 5 2806
BsrGI T'GTAC_A
Cuts at: 0 1214 2162 2811
Size: 1214 948 649
Fragments arranged by size:
1214 948 649
BstXI CCAn_nnnn'nTGG
Cuts at: 0 210 1245 2407 2811
Size: 210 1035 1162 404
Fragments arranged by size:
1162 1035 404 210
BstYI r'GATC_y
Cuts at: 0 849 912 1674 1699 2623 2811
Size: 849 63 762 25 924 188
Fragments arranged by size:
924 849 762 188 63 25
BtgI C'CryG_G
Cuts at: 0 18 905 1006 1764 2811
Size: 18 887 101 758 1047
Fragments arranged by size:
1047 887 758 101 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 719 761 2811
Size: 719 42 2050
Fragments arranged by size:
2050 719 42
BtsI GCAGTG_nn'
Cuts at: 0 231 461 1734 2538 2577 2811
Size: 231 230 1273 804 39 234
Fragments arranged by size:
1273 804 234 231 230 39
DraI TTT'AAA
Cuts at: 0 2217 2811
Size: 2217 594
DrdI GACnn_nn'nnGTC
Cuts at: 0 2258 2811
Size: 2258 553
EaeI y'GGCC_r
Cuts at: 0 7 50 65 1235 1247 2595 2811
Size: 7 43 15 1170 12 1348 216
Fragments arranged by size:
1348 1170 216 43 15 12 7
EagI C'GGCC_G
Cuts at: 0 7 2811
Size: 7 2804
EarI CTCTTCn'nnn_
Cuts at: 0 1267 2013 2811
Size: 1267 746 798
Fragments arranged by size:
1267 798 746
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 449 784 2003 2053 2162 2267 2811
Size: 449 335 1219 50 109 105 544
Fragments arranged by size:
1219 544 449 335 109 105 50
EcoO109I rG'GnC_Cy
Cuts at: 0 1842 2518 2811
Size: 1842 676 293
Fragments arranged by size:
1842 676 293
EcoRI G'AATT_C
Cuts at: 0 899 1011 2811
Size: 899 112 1800
Fragments arranged by size:
1800 899 112
EcoRV GAT'ATC
Cuts at: 0 2022 2356 2811
Size: 2022 334 455
Fragments arranged by size:
2022 455 334
HaeII r_GCGC'y
Cuts at: 0 46 996 2811
Size: 46 950 1815
Fragments arranged by size:
1815 950 46
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2011 2043 2811
Size: 2011 32 768
Fragments arranged by size:
2011 768 32
HincII GTy'rAC
Cuts at: 0 2585 2811
Size: 2585 226
KasI G'GCGC_C
Cuts at: 0 992 2811
Size: 992 1819
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1264 2811
Size: 1264 1547
MscI TGG'CCA
Cuts at: 0 1237 1249 2597 2811
Size: 1237 12 1348 214
Fragments arranged by size:
1348 1237 214 12
MslI CAynn'nnrTG
Cuts at: 0 952 1222 1712 2681 2811
Size: 952 270 490 969 130
Fragments arranged by size:
969 952 490 270 130
MspA1I CmG'CkG
Cuts at: 0 20 269 610 1503 1801 1927 2497 2811
Size: 20 249 341 893 298 126 570 314
Fragments arranged by size:
893 570 341 314 298 249 126 20
NarI GG'CG_CC
Cuts at: 0 993 2811
Size: 993 1818
NcoI C'CATG_G
Cuts at: 0 905 1006 2811
Size: 905 101 1805
Fragments arranged by size:
1805 905 101
NruI TCG'CGA
Cuts at: 0 746 2811
Size: 746 2065
NsiI A_TGCA'T
Cuts at: 0 1374 2811
Size: 1374 1437
NspI r_CATG'y
Cuts at: 0 533 582 1650 2370 2664 2811
Size: 533 49 1068 720 294 147
Fragments arranged by size:
1068 720 533 294 147 49
PasI CC'CwG_GG
Cuts at: 0 1093 2811
Size: 1093 1718
PciI A'CATG_T
Cuts at: 0 2366 2660 2811
Size: 2366 294 151
Fragments arranged by size:
2366 294 151
PflMI CCAn_nnn'nTGG
Cuts at: 0 269 393 1970 2080 2811
Size: 269 124 1577 110 731
Fragments arranged by size:
1577 731 269 124 110
PfoI T'CCnGG_A
Cuts at: 0 296 471 761 2475 2811
Size: 296 175 290 1714 336
Fragments arranged by size:
1714 336 296 290 175
PpuMI rG'GwC_Cy
Cuts at: 0 1842 2518 2811
Size: 1842 676 293
Fragments arranged by size:
1842 676 293
PshAI GACnn'nnGTC
Cuts at: 0 740 2811
Size: 740 2071
PsiI TTA'TAA
Cuts at: 0 941 2811
Size: 941 1870
PstI C_TGCA'G
Cuts at: 0 1726 2805 2811
Size: 1726 1079 6
Fragments arranged by size:
1726 1079 6
PvuII CAG'CTG
Cuts at: 0 610 1503 1801 2497 2811
Size: 610 893 298 696 314
Fragments arranged by size:
893 696 610 314 298
SacII CC_GC'GG
Cuts at: 0 21 2811
Size: 21 2790
SalI G'TCGA_C
Cuts at: 0 2583 2811
Size: 2583 228
SfcI C'TryA_G
Cuts at: 0 1722 1935 2801 2811
Size: 1722 213 866 10
Fragments arranged by size:
1722 866 213 10
SfoI GGC'GCC
Cuts at: 0 994 2811
Size: 994 1817
SmlI C'TyrA_G
Cuts at: 0 144 2256 2811
Size: 144 2112 555
Fragments arranged by size:
2112 555 144
SpeI A'CTAG_T
Cuts at: 0 1221 2811
Size: 1221 1590
SphI G_CATG'C
Cuts at: 0 1650 2811
Size: 1650 1161
SspI AAT'ATT
Cuts at: 0 1602 1860 2643 2772 2811
Size: 1602 258 783 129 39
Fragments arranged by size:
1602 783 258 129 39
StyI C'CwwG_G
Cuts at: 0 905 1006 1127 1187 2811
Size: 905 101 121 60 1624
Fragments arranged by size:
1624 905 121 101 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 311 1426 2169 2228 2549 2811
Size: 311 1115 743 59 321 262
Fragments arranged by size:
1115 743 321 311 262 59
TatI w'GTAC_w
Cuts at: 0 788 1214 2162 2811
Size: 788 426 948 649
Fragments arranged by size:
948 788 649 426
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 453 485 516 548 960 992 2811
Size: 453 32 31 32 412 32 1819
Fragments arranged by size:
1819 453 412 32 32 32 31
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2077 2811
Size: 2077 734
Enzymes that do cut:
AccI AcuI AflIII AleI AlwNI ApoI AseI AvaI
BamHI BanI BanII BbeI BbsI BciVI BfrBI BglI
BglII BlpI BmgBI BmrI BplI BpmI BpuEI BsaI
BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsmI BsmBI
Bsp1286I BspEI BspHI BsrBI BsrDI BsrFI BsrGI BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI Eco57MI EcoO109I EcoRI EcoRV HaeII Hin4I HincII
KasI MmeI MscI MslI MspA1I NarI NcoI NruI
NsiI NspI PasI PciI PflMI PfoI PpuMI PshAI
PsiI PstI PvuII SacII SalI SfcI SfoI SmlI
SpeI SphI SspI StyI TaqII TatI TstI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AlfI AloI ApaI ApaLI AscI AsiSI AvrII BaeI
BbvCI BcgI BclI Bme1580I BmtI Bpu10I BsaAI BsaBI
BseRI BsiHKAI BsiWI BspMI BssHII BssSI BstAPI BstBI
BstEII BstZ17I Bsu36I ClaI CspCI DraIII EciI EcoICRI
EcoNI FalI FseI FspI FspAI HindIII HpaI KpnI
MfeI MluI NaeI NdeI NgoMIV NheI NotI PacI
PmeI PmlI PpiI PspOMI PspXI PsrI PvuI RsrII
SacI SanDI SapI SbfI ScaI SexAI SfiI SgfI
SgrAI SmaI SnaBI SrfI StuI SwaI Tth111I XbaI
XhoI XmaI XmnI ZraI