Restriction map for
pFN21AE3325
(Linear) (Six-base) MAPSORT of: KIEE3325.seq Check: 2636 from: 1 to: 2763
KIEE3325.orf
With 182 enzymes: SgfI *
July 3, 2012 17:39 ..
AatII G_ACGT'C
Cuts at: 0 1489 2483 2763
Size: 1489 994 280
Fragments arranged by size:
1489 994 280
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 96 787 1864 2316 2763
Size: 96 691 1077 452 447
Fragments arranged by size:
1077 691 452 447 96
AfeI AGC'GCT
Cuts at: 0 979 1000 2763
Size: 979 21 1763
Fragments arranged by size:
1763 979 21
AflIII A'CryG_T
Cuts at: 0 377 2763
Size: 377 2386
AhdI GACnn_n'nnGTC
Cuts at: 0 2274 2763
Size: 2274 489
AleI CACnn'nnGTG
Cuts at: 0 580 721 2763
Size: 580 141 2042
Fragments arranged by size:
2042 580 141
AlwNI CAG_nnn'CTG
Cuts at: 0 2135 2763
Size: 2135 628
ApaI G_GGCC'C
Cuts at: 0 567 2763
Size: 567 2196
ApaLI G'TGCA_C
Cuts at: 0 1185 1566 2763
Size: 1185 381 1197
Fragments arranged by size:
1197 1185 381
AvaI C'yCGr_G
Cuts at: 0 559 609 774 819 1670 2763
Size: 559 50 165 45 851 1093
Fragments arranged by size:
1093 851 559 165 50 45
BamHI G'GATC_C
Cuts at: 0 482 2763
Size: 482 2281
BanI G'GyrC_C
Cuts at: 0 869 1647 1653 1674 2092 2409 2763
Size: 869 778 6 21 418 317 354
Fragments arranged by size:
869 778 418 354 317 21 6
BanII G_rGCy'C
Cuts at: 0 473 508 567 1925 2107 2763
Size: 473 35 59 1358 182 656
Fragments arranged by size:
1358 656 473 182 59 35
BbeI G_GCGC'C
Cuts at: 0 1678 2763
Size: 1678 1085
BbsI GAAGACnn'nnnn_
Cuts at: 0 2280 2763
Size: 2280 483
BbvCI CC'TCA_GC
Cuts at: 0 2593 2763
Size: 2593 170
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1102 1136 2763
Size: 1102 34 1627
Fragments arranged by size:
1627 1102 34
BclI T'GATC_A
Cuts at: 0 1289 2763
Size: 1289 1474
BglI GCCn_nnn'nGGC
Cuts at: 0 2603 2763
Size: 2603 160
BglII A'GATC_T
Cuts at: 0 1128 2763
Size: 1128 1635
BlpI GC'TnA_GC
Cuts at: 0 253 2763
Size: 253 2510
Bme1580I G_kGCm'C
Cuts at: 0 339 463 567 622 739 872 1189 1570
Size: 339 124 104 55 117 133 317 381
Cuts at: 1570 2575 2763
Size: 1005 188
Fragments arranged by size:
1005 381 339 317 188 133 124 117
104 55
BmgBI CAC'GTC
Cuts at: 0 1184 2763
Size: 1184 1579
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 255 2126 2763
Size: 255 1871 637
Fragments arranged by size:
1871 637 255
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 250 280 655 1446 1930 2716 2763
Size: 109 141 30 375 791 484 786 47
Fragments arranged by size:
791 786 484 375 141 109 47 30
Bpu10I CC'TnA_GC
Cuts at: 0 34 2593 2763
Size: 34 2559 170
Fragments arranged by size:
2559 170 34
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 30 913 1204 2008 2198 2257 2763
Size: 30 883 291 804 190 59 506
Fragments arranged by size:
883 804 506 291 190 59 30
BsaI GGTCTCn'nnnn_
Cuts at: 0 2658 2763
Size: 2658 105
BsaAI yAC'GTr
Cuts at: 0 582 2763
Size: 582 2181
BsaHI Gr'CG_yC
Cuts at: 0 602 1486 1663 1675 2480 2763
Size: 602 884 177 12 805 283
Fragments arranged by size:
884 805 602 283 177 12
BsaWI w'CCGG_w
Cuts at: 0 80 1322 1695 2763
Size: 80 1242 373 1068
Fragments arranged by size:
1242 1068 373 80
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1462 1492 1502 1532 1741 1771 2763
Size: 1462 30 10 30 209 30 992
Fragments arranged by size:
1462 992 209 30 30 30 10
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 343 783 1316 1467 1491 1819 1825 2461
Size: 343 440 533 151 24 328 6 636
Cuts at: 2461 2763
Size: 302
Fragments arranged by size:
636 533 440 343 328 302 151 24
6
BseYI C'CCAG_C
Cuts at: 0 182 864 2642 2763
Size: 182 682 1778 121
Fragments arranged by size:
1778 682 182 121
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1372 2763
Size: 1372 1391
BsiEI CG_ry'CG
Cuts at: 0 1962 2763
Size: 1962 801
BsiHKAI G_wGCw'C
Cuts at: 0 1189 1570 1925 2763
Size: 1189 381 355 838
Fragments arranged by size:
1189 838 381 355
BsmBI CGTCTCn'nnnn_
Cuts at: 0 427 1654 2763
Size: 427 1227 1109
Fragments arranged by size:
1227 1109 427
Bsp1286I G_dGCh'C
Cuts at: 0 339 463 473 508 567 622 739 872
Size: 339 124 10 35 59 55 117 133
Cuts at: 872 1189 1570 1925 2107 2575 2763
Size: 317 381 355 182 468 188
Fragments arranged by size:
468 381 355 339 317 188 182 133
124 117 59 55 35 10
BspEI T'CCGG_A
Cuts at: 0 80 1322 2763
Size: 80 1242 1441
Fragments arranged by size:
1441 1242 80
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1394 1601 2763
Size: 1394 207 1162
Fragments arranged by size:
1394 1162 207
BsrBI CCG'CTC
Cuts at: 0 1010 2763
Size: 1010 1753
BsrFI r'CCGG_y
Cuts at: 0 116 1650 2763
Size: 116 1534 1113
Fragments arranged by size:
1534 1113 116
BsrGI T'GTAC_A
Cuts at: 0 572 2763
Size: 572 2191
BssSI C'ACGA_G
Cuts at: 0 853 2763
Size: 853 1910
BstEII G'GTnAC_C
Cuts at: 0 1698 2763
Size: 1698 1065
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2685 2763
Size: 2685 78
BstYI r'GATC_y
Cuts at: 0 482 932 1115 1128 1305 2763
Size: 482 450 183 13 177 1458
Fragments arranged by size:
1458 482 450 183 177 13
Bsu36I CC'TnA_GG
Cuts at: 0 364 2763
Size: 364 2399
BtgI C'CryG_G
Cuts at: 0 478 596 1956 2763
Size: 478 118 1360 807
Fragments arranged by size:
1360 807 478 118
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 647 2763
Size: 647 2116
DraIII CAC_nnn'GTG
Cuts at: 0 479 876 2763
Size: 479 397 1887
Fragments arranged by size:
1887 479 397
EaeI y'GGCC_r
Cuts at: 0 246 1959 1979 2117 2763
Size: 246 1713 20 138 646
Fragments arranged by size:
1713 646 246 138 20
EagI C'GGCC_G
Cuts at: 0 1959 2763
Size: 1959 804
EarI CTCTTCn'nnn_
Cuts at: 0 904 1763 1784 2335 2641 2654 2763
Size: 904 859 21 551 306 13 109
Fragments arranged by size:
904 859 551 306 109 21 13
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2413 2763
Size: 2413 350
EcoICRI GAG'CTC
Cuts at: 0 1923 2763
Size: 1923 840
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 96 109 250 280 655 787 1446 1864
Size: 96 13 141 30 375 132 659 418
Cuts at: 1864 1930 2316 2716 2763
Size: 66 386 400 47
Fragments arranged by size:
659 418 400 386 375 141 132 96
66 47 30 13
EcoNI CCTnn'n_nnAGG
Cuts at: 0 363 2137 2263 2763
Size: 363 1774 126 500
Fragments arranged by size:
1774 500 363 126
EcoO109I rG'GnC_Cy
Cuts at: 0 225 264 513 563 564 779 2268 2763
Size: 225 39 249 50 1 215 1489 495
Fragments arranged by size:
1489 495 249 225 215 50 39 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 162 1225 1956 2304 2478 2763
Size: 162 1063 731 348 174 285
Fragments arranged by size:
1063 731 348 285 174 162
FspI TGC'GCA
Cuts at: 0 217 2380 2763
Size: 217 2163 383
Fragments arranged by size:
2163 383 217
HaeII r_GCGC'y
Cuts at: 0 981 1002 1678 2763
Size: 981 21 676 1085
Fragments arranged by size:
1085 981 676 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1692 1724 1741 1773 2135 2167 2260 2292
Size: 1692 32 17 32 362 32 93 32
Cuts at: 2292 2763
Size: 471
Fragments arranged by size:
1692 471 362 93 32 32 32 32
17
HincII GTy'rAC
Cuts at: 0 2193 2763
Size: 2193 570
KasI G'GCGC_C
Cuts at: 0 1674 2763
Size: 1674 1089
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 44 80 1739 2763
Size: 44 36 1659 1024
Fragments arranged by size:
1659 1024 44 36
MscI TGG'CCA
Cuts at: 0 1981 2119 2763
Size: 1981 138 644
Fragments arranged by size:
1981 644 138
MslI CAynn'nnrTG
Cuts at: 0 580 721 2578 2689 2763
Size: 580 141 1857 111 74
Fragments arranged by size:
1857 580 141 111 74
MspA1I CmG'CkG
Cuts at: 0 123 252 675 864 953 987 1908 1964
Size: 123 129 423 189 89 34 921 56
Cuts at: 1964 2430 2763
Size: 466 333
Fragments arranged by size:
921 466 423 333 189 129 123 89
56 34
NaeI GCC'GGC
Cuts at: 0 118 2763
Size: 118 2645
NarI GG'CG_CC
Cuts at: 0 1675 2763
Size: 1675 1088
NgoMIV G'CCGG_C
Cuts at: 0 116 2763
Size: 116 2647
NspI r_CATG'y
Cuts at: 0 381 2694 2763
Size: 381 2313 69
Fragments arranged by size:
2313 381 69
PasI CC'CwG_GG
Cuts at: 0 509 2763
Size: 509 2254
PciI A'CATG_T
Cuts at: 0 377 2763
Size: 377 2386
PflMI CCAn_nnn'nTGG
Cuts at: 0 419 479 1169 2525 2763
Size: 419 60 690 1356 238
Fragments arranged by size:
1356 690 419 238 60
PfoI T'CCnGG_A
Cuts at: 0 227 2763
Size: 227 2536
PmlI CAC'GTG
Cuts at: 0 582 2763
Size: 582 2181
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 952 984 2763
Size: 952 32 1779
Fragments arranged by size:
1779 952 32
PpuMI rG'GwC_Cy
Cuts at: 0 225 264 513 779 2268 2763
Size: 225 39 249 266 1489 495
Fragments arranged by size:
1489 495 266 249 225 39
PspOMI G'GGCC_C
Cuts at: 0 563 2763
Size: 563 2200
PstI C_TGCA'G
Cuts at: 0 2354 2757 2763
Size: 2354 403 6
Fragments arranged by size:
2354 403 6
PvuII CAG'CTG
Cuts at: 0 123 252 864 953 1908 2430 2763
Size: 123 129 612 89 955 522 333
Fragments arranged by size:
955 612 522 333 129 123 89
RsrII CG'GwC_CG
Cuts at: 0 684 2763
Size: 684 2079
SacI G_AGCT'C
Cuts at: 0 1925 2763
Size: 1925 838
SanDI GG'GwC_CC
Cuts at: 0 513 779 2763
Size: 513 266 1984
Fragments arranged by size:
1984 513 266
SapI GCTCTTCn'nnn_
Cuts at: 0 904 2763
Size: 904 1859
ScaI AGT'ACT
Cuts at: 0 109 1168 2763
Size: 109 1059 1595
Fragments arranged by size:
1595 1059 109
SfcI C'TryA_G
Cuts at: 0 2052 2350 2753 2763
Size: 2052 298 403 10
Fragments arranged by size:
2052 403 298 10
SfoI GGC'GCC
Cuts at: 0 1676 2763
Size: 1676 1087
SmaI CCC'GGG
Cuts at: 0 561 611 776 821 1672 2763
Size: 561 50 165 45 851 1091
Fragments arranged by size:
1091 851 561 165 50 45
SmlI C'TyrA_G
Cuts at: 0 9 928 1219 2023 2213 2272 2763
Size: 9 919 291 804 190 59 491
Fragments arranged by size:
919 804 491 291 190 59 9
StuI AGG'CCT
Cuts at: 0 2029 2763
Size: 2029 734
StyI C'CwwG_G
Cuts at: 0 2249 2490 2763
Size: 2249 241 273
Fragments arranged by size:
2249 273 241
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 505 592 2080 2763
Size: 505 87 1488 683
Fragments arranged by size:
1488 683 505 87
TatI w'GTAC_w
Cuts at: 0 107 572 1166 2763
Size: 107 465 594 1597
Fragments arranged by size:
1597 594 465 107
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 30 62 1141 1173 1502 1534 2763
Size: 30 32 1079 32 329 32 1229
Fragments arranged by size:
1229 1079 329 32 32 32 30
Tth111I GACn'n_nGTC
Cuts at: 0 1329 2763
Size: 1329 1434
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2181 2763
Size: 2181 582
XmaI C'CCGG_G
Cuts at: 0 559 609 774 819 1670 2763
Size: 559 50 165 45 851 1093
Fragments arranged by size:
1093 851 559 165 50 45
XmnI GAAnn'nnTTC
Cuts at: 0 109 1585 2287 2763
Size: 109 1476 702 476
Fragments arranged by size:
1476 702 476 109
ZraI GAC'GTC
Cuts at: 0 1487 2481 2763
Size: 1487 994 282
Fragments arranged by size:
1487 994 282
Enzymes that do cut:
AatII AcuI AfeI AflIII AhdI AleI AlwNI ApaI
ApaLI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BclI BglI BglII BlpI Bme1580I BmgBI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspMI BsrBI BsrFI BsrGI BssSI BstEII BstXI BstYI
Bsu36I BtgI BtgZI DraIII EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I
HincII KasI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PasI PciI PflMI PfoI PmlI PpiI
PpuMI PspOMI PstI PvuII RsrII SacI SanDI SapI
ScaI SfcI SfoI SmaI SmlI StuI StyI TaqII
TatI TstI Tth111I XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AflII AgeI AlfI AloI
ApoI AscI AseI AsiSI AvrII BaeI BciVI BfrBI
BmrI BmtI BsaBI BsiWI BsmI BspHI BsrDI BssHII
BstAPI BstBI BstZ17I BtsI ClaI CspCI DraI DrdI
EcoRI EcoRV FseI FspAI HindIII HpaI KpnI MfeI
MluI NcoI NdeI NheI NotI NruI NsiI PacI
PmeI PshAI PsiI PspXI PsrI PvuI SacII SalI
SbfI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI SwaI XbaI XhoI