Restriction map for
pFN21AE3323
(Linear) (Six-base) MAPSORT of: KIEE3323.seq Check: 1730 from: 1 to: 2751
KIEE3323.orf
With 182 enzymes: SgfI *
March 22, 2012 16:29 ..
AccI GT'mk_AC
Cuts at: 0 1617 2192 2751
Size: 1617 575 559
Fragments arranged by size:
1617 575 559
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 286 1879 2141 2309 2335 2751
Size: 286 1593 262 168 26 416
Fragments arranged by size:
1593 416 286 262 168 26
AflIII A'CryG_T
Cuts at: 0 498 2751
Size: 498 2253
AgeI A'CCGG_T
Cuts at: 0 1636 2751
Size: 1636 1115
ApaI G_GGCC'C
Cuts at: 0 130 150 175 2751
Size: 130 20 25 2576
Fragments arranged by size:
2576 130 25 20
ApoI r'AATT_y
Cuts at: 0 653 1324 1727 2283 2439 2725 2751
Size: 653 671 403 556 156 286 26
Fragments arranged by size:
671 653 556 403 286 156 26
AseI AT'TA_AT
Cuts at: 0 962 1394 2140 2751
Size: 962 432 746 611
Fragments arranged by size:
962 746 611 432
AvaI C'yCGr_G
Cuts at: 0 174 204 211 305 704 2751
Size: 174 30 7 94 399 2047
Fragments arranged by size:
2047 399 174 94 30 7
BanI G'GyrC_C
Cuts at: 0 300 1383 1521 2751
Size: 300 1083 138 1230
Fragments arranged by size:
1230 1083 300 138
BanII G_rGCy'C
Cuts at: 0 130 150 175 254 926 2751
Size: 130 20 25 79 672 1825
Fragments arranged by size:
1825 672 130 79 25 20
BbsI GAAGACnn'nnnn_
Cuts at: 0 1289 2315 2751
Size: 1289 1026 436
Fragments arranged by size:
1289 1026 436
BbvCI CC'TCA_GC
Cuts at: 0 218 860 2751
Size: 218 642 1891
Fragments arranged by size:
1891 642 218
BciVI GTATCCnnnnn_n'
Cuts at: 0 501 2574 2751
Size: 501 2073 177
Fragments arranged by size:
2073 501 177
BglI GCCn_nnn'nGGC
Cuts at: 0 134 2751
Size: 134 2617
BglII A'GATC_T
Cuts at: 0 423 2751
Size: 423 2328
BlpI GC'TnA_GC
Cuts at: 0 319 925 2751
Size: 319 606 1826
Fragments arranged by size:
1826 606 319
Bme1580I G_kGCm'C
Cuts at: 0 112 130 150 175 305 1557 2751
Size: 112 18 20 25 130 1252 1194
Fragments arranged by size:
1252 1194 130 112 25 20 18
BmrI ACTGGGnnnn_n'
Cuts at: 0 1130 2751
Size: 1130 1621
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 926 2751
Size: 926 1825
Bpu10I CC'TnA_GC
Cuts at: 0 218 860 2751
Size: 218 642 1891
Fragments arranged by size:
1891 642 218
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 388 1384 1939 2751
Size: 388 996 555 812
Fragments arranged by size:
996 812 555 388
BsaBI GATnn'nnATC
Cuts at: 0 512 2751
Size: 512 2239
BsaWI w'CCGG_w
Cuts at: 0 1636 2751
Size: 1636 1115
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1596 1626 1883 1896 1913 1926 2751
Size: 1596 30 257 13 17 13 825
Fragments arranged by size:
1596 825 257 30 17 13 13
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 88 349 716 1891 1904 2751
Size: 88 261 367 1175 13 847
Fragments arranged by size:
1175 847 367 261 88 13
BseYI C'CCAG_C
Cuts at: 0 168 2751
Size: 168 2583
BsiEI CG_ry'CG
Cuts at: 0 1551 2751
Size: 1551 1200
BsiHKAI G_wGCw'C
Cuts at: 0 926 2751
Size: 926 1825
BsmI GAATG_Cn'
Cuts at: 0 1055 2172 2751
Size: 1055 1117 579
Fragments arranged by size:
1117 1055 579
Bsp1286I G_dGCh'C
Cuts at: 0 112 130 150 175 254 305 926 1557
Size: 112 18 20 25 79 51 621 631
Cuts at: 1557 2751
Size: 1194
Fragments arranged by size:
1194 631 621 112 79 51 25 20
18
BspHI T'CATG_A
Cuts at: 0 729 2510 2751
Size: 729 1781 241
Fragments arranged by size:
1781 729 241
BsrBI CCG'CTC
Cuts at: 0 60 1593 2751
Size: 60 1533 1158
Fragments arranged by size:
1533 1158 60
BsrDI GCAATG_nn'
Cuts at: 0 1583 2751
Size: 1583 1168
BsrFI r'CCGG_y
Cuts at: 0 1636 2751
Size: 1636 1115
BsrGI T'GTAC_A
Cuts at: 0 1424 2568 2751
Size: 1424 1144 183
Fragments arranged by size:
1424 1144 183
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1338 2751
Size: 1338 1413
BstYI r'GATC_y
Cuts at: 0 423 2751
Size: 423 2328
BstZ17I GTA'TAC
Cuts at: 0 2193 2751
Size: 2193 558
Bsu36I CC'TnA_GG
Cuts at: 0 971 2751
Size: 971 1780
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 83 2751
Size: 83 2668
ClaI AT'CG_AT
Cuts at: 0 2415 2751
Size: 2415 336
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2102 2137 2751
Size: 2102 35 614
Fragments arranged by size:
2102 614 35
DraIII CAC_nnn'GTG
Cuts at: 0 2718 2751
Size: 2718 33
EarI CTCTTCn'nnn_
Cuts at: 0 44 50 113 914 2751
Size: 44 6 63 801 1837
Fragments arranged by size:
1837 801 63 44 6
EcoICRI GAG'CTC
Cuts at: 0 924 2751
Size: 924 1827
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 286 926 1879 2141 2309 2335 2751
Size: 286 640 953 262 168 26 416
Fragments arranged by size:
953 640 416 286 262 168 26
EcoNI CCTnn'n_nnAGG
Cuts at: 0 746 2751
Size: 746 2005
EcoO109I rG'GnC_Cy
Cuts at: 0 127 146 147 2088 2751
Size: 127 19 1 1941 663
Fragments arranged by size:
1941 663 127 19 1
EcoRI G'AATT_C
Cuts at: 0 2439 2751
Size: 2439 312
EcoRV GAT'ATC
Cuts at: 0 2152 2751
Size: 2152 599
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 442 772 1378 1780 2751
Size: 442 330 606 402 971
Fragments arranged by size:
971 606 442 402 330
FspI TGC'GCA
Cuts at: 0 31 2751
Size: 31 2720
HaeII r_GCGC'y
Cuts at: 0 696 2751
Size: 696 2055
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 518 550 1081 1113 1334 1366 1596 1628
Size: 518 32 531 32 221 32 230 32
Cuts at: 1628 1635 1667 1883 1915 2408 2440 2523 2555
Size: 7 32 216 32 493 32 83 32
Cuts at: 2555 2751
Size: 196
Fragments arranged by size:
531 518 493 230 221 216 196 83
32 32 32 32 32 32 32 32
7
HincII GTy'rAC
Cuts at: 0 988 2751
Size: 988 1763
MfeI C'AATT_G
Cuts at: 0 1781 2487 2751
Size: 1781 706 264
Fragments arranged by size:
1781 706 264
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1175 2751
Size: 1175 1576
MslI CAynn'nnrTG
Cuts at: 0 734 2751
Size: 734 2017
MspA1I CmG'CkG
Cuts at: 0 466 1422 2751
Size: 466 956 1329
Fragments arranged by size:
1329 956 466
NruI TCG'CGA
Cuts at: 0 287 2751
Size: 287 2464
NspI r_CATG'y
Cuts at: 0 502 679 1573 2751
Size: 502 177 894 1178
Fragments arranged by size:
1178 894 502 177
PciI A'CATG_T
Cuts at: 0 498 2751
Size: 498 2253
PflMI CCAn_nnn'nTGG
Cuts at: 0 598 2504 2751
Size: 598 1906 247
Fragments arranged by size:
1906 598 247
PfoI T'CCnGG_A
Cuts at: 0 326 645 2751
Size: 326 319 2106
Fragments arranged by size:
2106 326 319
PpuMI rG'GwC_Cy
Cuts at: 0 2088 2751
Size: 2088 663
PspOMI G'GGCC_C
Cuts at: 0 126 146 171 2751
Size: 126 20 25 2580
Fragments arranged by size:
2580 126 25 20
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2592 2624 2751
Size: 2592 32 127
Fragments arranged by size:
2592 127 32
PvuI CG_AT'CG
Cuts at: 0 1551 2751
Size: 1551 1200
PvuII CAG'CTG
Cuts at: 0 466 1422 2751
Size: 466 956 1329
Fragments arranged by size:
1329 956 466
SacI G_AGCT'C
Cuts at: 0 926 2751
Size: 926 1825
SanDI GG'GwC_CC
Cuts at: 0 2088 2751
Size: 2088 663
SapI GCTCTTCn'nnn_
Cuts at: 0 50 914 2751
Size: 50 864 1837
Fragments arranged by size:
1837 864 50
SfcI C'TryA_G
Cuts at: 0 1691 2751
Size: 1691 1060
SmaI CCC'GGG
Cuts at: 0 176 206 307 2751
Size: 176 30 101 2444
Fragments arranged by size:
2444 176 101 30
SmlI C'TyrA_G
Cuts at: 0 403 1363 1918 2751
Size: 403 960 555 833
Fragments arranged by size:
960 833 555 403
SphI G_CATG'C
Cuts at: 0 1573 2751
Size: 1573 1178
StuI AGG'CCT
Cuts at: 0 282 1664 2751
Size: 282 1382 1087
Fragments arranged by size:
1382 1087 282
TatI w'GTAC_w
Cuts at: 0 1007 1424 2568 2751
Size: 1007 417 1144 183
Fragments arranged by size:
1144 1007 417 183
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1896 1928 2751
Size: 1896 32 823
Fragments arranged by size:
1896 823 32
XmaI C'CCGG_G
Cuts at: 0 174 204 305 2751
Size: 174 30 101 2446
Fragments arranged by size:
2446 174 101 30
Enzymes that do cut:
AccI AcuI AflIII AgeI ApaI ApoI AseI AvaI
BanI BanII BbsI BbvCI BciVI BglI BglII BlpI
Bme1580I BmrI BpmI Bpu10I BpuEI BsaBI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspHI BsrBI
BsrDI BsrFI BsrGI BstAPI BstYI BstZ17I Bsu36I BtgZI
ClaI CspCI DraIII EarI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII MfeI
MmeI MslI MspA1I NruI NspI PciI PflMI PfoI
PpuMI PspOMI PsrI PvuI PvuII SacI SanDI SapI
SfcI SmaI SmlI SphI StuI TatI TstI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AhdI AleI
AlfI AloI AlwNI ApaLI AscI AsiSI AvrII BaeI
BamHI BbeI BcgI BclI BfrBI BmgBI BmtI BplI
BsaI BsaAI BsaHI BsgI BsiWI BsmBI BspEI BspMI
BssHII BssSI BstBI BstEII BstXI BtgI BtsI DraI
DrdI EaeI EagI EciI FseI FspAI HindIII HpaI
KasI KpnI MluI MscI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NsiI PacI PasI PmeI PmlI
PpiI PshAI PsiI PspXI PstI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI
SpeI SrfI SspI StyI SwaI TaqII Tth111I XbaI
XcmI XhoI XmnI ZraI