Restriction map for
pFN21AE3322
(Linear) (Six-base) MAPSORT of: KIEE3322.seq Check: 9943 from: 1 to: 2739
KIEE3322.orf
With 182 enzymes: SgfI *
December 5, 2014 12:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 218 2739
Size: 218 2521
AccI GT'mk_AC
Cuts at: 0 1441 1922 2739
Size: 1441 481 817
Fragments arranged by size:
1441 817 481
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 448 2713 2739
Size: 448 2265 26
Fragments arranged by size:
2265 448 26
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1352 2739
Size: 1352 1387
AlwNI CAG_nnn'CTG
Cuts at: 0 271 1111 2265 2739
Size: 271 840 1154 474
Fragments arranged by size:
1154 840 474 271
ApaLI G'TGCA_C
Cuts at: 0 1519 2739
Size: 1519 1220
ApoI r'AATT_y
Cuts at: 0 577 995 1228 1893 2180 2355 2739
Size: 577 418 233 665 287 175 384
Fragments arranged by size:
665 577 418 384 287 233 175
BanI G'GyrC_C
Cuts at: 0 1660 2290 2739
Size: 1660 630 449
Fragments arranged by size:
1660 630 449
BbsI GAAGACnn'nnnn_
Cuts at: 0 751 1634 1872 2509 2739
Size: 751 883 238 637 230
Fragments arranged by size:
883 751 637 238 230
BbvCI CC'TCA_GC
Cuts at: 0 2255 2739
Size: 2255 484
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 611 645 2739
Size: 611 34 2094
Fragments arranged by size:
2094 611 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2602 2739
Size: 2602 137
BfrBI ATG'CAT
Cuts at: 0 716 2652 2739
Size: 716 1936 87
Fragments arranged by size:
1936 716 87
BglII A'GATC_T
Cuts at: 0 2555 2660 2739
Size: 2555 105 79
Fragments arranged by size:
2555 105 79
Bme1580I G_kGCm'C
Cuts at: 0 1523 2078 2303 2739
Size: 1523 555 225 436
Fragments arranged by size:
1523 555 436 225
BmrI ACTGGGnnnn_n'
Cuts at: 0 59 2380 2639 2739
Size: 59 2321 259 100
Fragments arranged by size:
2321 259 100 59
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2036 2739
Size: 2036 703
Bpu10I CC'TnA_GC
Cuts at: 0 2255 2739
Size: 2255 484
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1351 1495 1701 2739
Size: 1351 144 206 1038
Fragments arranged by size:
1351 1038 206 144
BsaI GGTCTCn'nnnn_
Cuts at: 0 324 2716 2739
Size: 324 2392 23
Fragments arranged by size:
2392 324 23
BsaAI yAC'GTr
Cuts at: 0 19 2739
Size: 19 2720
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2734 2739
Size: 2734 5
BseYI C'CCAG_C
Cuts at: 0 2696 2739
Size: 2696 43
BsiHKAI G_wGCw'C
Cuts at: 0 1502 1523 2739
Size: 1502 21 1216
Fragments arranged by size:
1502 1216 21
BsmI GAATG_Cn'
Cuts at: 0 73 551 924 1939 2697 2739
Size: 73 478 373 1015 758 42
Fragments arranged by size:
1015 758 478 373 73 42
Bsp1286I G_dGCh'C
Cuts at: 0 1502 1523 2078 2303 2739
Size: 1502 21 555 225 436
Fragments arranged by size:
1502 555 436 225 21
BspHI T'CATG_A
Cuts at: 0 719 1149 2739
Size: 719 430 1590
Fragments arranged by size:
1590 719 430
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 218 2034 2739
Size: 218 1816 705
Fragments arranged by size:
1816 705 218
BsrDI GCAATG_nn'
Cuts at: 0 1137 1334 1647 1659 2739
Size: 1137 197 313 12 1080
Fragments arranged by size:
1137 1080 313 197 12
BsrFI r'CCGG_y
Cuts at: 0 2471 2739
Size: 2471 268
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2265 2739
Size: 2265 474
BstBI TT'CG_AA
Cuts at: 0 1728 2739
Size: 1728 1011
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1023 2014 2739
Size: 1023 991 725
Fragments arranged by size:
1023 991 725
BstYI r'GATC_y
Cuts at: 0 2555 2660 2739
Size: 2555 105 79
Fragments arranged by size:
2555 105 79
BstZ17I GTA'TAC
Cuts at: 0 1923 2739
Size: 1923 816
BtgI C'CryG_G
Cuts at: 0 882 985 1125 2739
Size: 882 103 140 1614
Fragments arranged by size:
1614 882 140 103
BtsI GCAGTG_nn'
Cuts at: 0 92 929 2227 2263 2739
Size: 92 837 1298 36 476
Fragments arranged by size:
1298 837 476 92 36
DraI TTT'AAA
Cuts at: 0 1044 1634 1851 2739
Size: 1044 590 217 888
Fragments arranged by size:
1044 888 590 217
DraIII CAC_nnn'GTG
Cuts at: 0 833 2739
Size: 833 1906
DrdI GACnn_nn'nnGTC
Cuts at: 0 1612 2511 2739
Size: 1612 899 228
Fragments arranged by size:
1612 899 228
EarI CTCTTCn'nnn_
Cuts at: 0 529 677 1463 1679 2663 2681 2739
Size: 529 148 786 216 984 18 58
Fragments arranged by size:
984 786 529 216 148 58 18
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 448 2036 2713 2739
Size: 448 1588 677 26
Fragments arranged by size:
1588 677 448 26
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2731 2739
Size: 2731 8
EcoRI G'AATT_C
Cuts at: 0 577 2180 2355 2739
Size: 577 1603 175 384
Fragments arranged by size:
1603 577 384 175
EcoRV GAT'ATC
Cuts at: 0 56 1788 2739
Size: 56 1732 951
Fragments arranged by size:
1732 951 56
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 306 2739
Size: 306 2433
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 397 429 1804 1836 1900 1932 2127 2159
Size: 397 32 1375 32 64 32 195 32
Cuts at: 2159 2739
Size: 580
Fragments arranged by size:
1375 580 397 195 64 32 32 32
32
HincII GTy'rAC
Cuts at: 0 2061 2739
Size: 2061 678
HindIII A'AGCT_T
Cuts at: 0 1346 2274 2739
Size: 1346 928 465
Fragments arranged by size:
1346 928 465
MfeI C'AATT_G
Cuts at: 0 277 661 2739
Size: 277 384 2078
Fragments arranged by size:
2078 384 277
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1494 2739
Size: 1494 1245
MslI CAynn'nnrTG
Cuts at: 0 1130 1652 2739
Size: 1130 522 1087
Fragments arranged by size:
1130 1087 522
MspA1I CmG'CkG
Cuts at: 0 1111 2739
Size: 1111 1628
NcoI C'CATG_G
Cuts at: 0 985 1125 2739
Size: 985 140 1614
Fragments arranged by size:
1614 985 140
NsiI A_TGCA'T
Cuts at: 0 718 2654 2739
Size: 718 1936 85
Fragments arranged by size:
1936 718 85
NspI r_CATG'y
Cuts at: 0 2652 2739
Size: 2652 87
PasI CC'CwG_GG
Cuts at: 0 2295 2739
Size: 2295 444
PflMI CCAn_nnn'nTGG
Cuts at: 0 1804 2739
Size: 1804 935
PfoI T'CCnGG_A
Cuts at: 0 2594 2739
Size: 2594 145
PshAI GACnn'nnGTC
Cuts at: 0 457 755 2739
Size: 457 298 1984
Fragments arranged by size:
1984 457 298
PstI C_TGCA'G
Cuts at: 0 1116 2739
Size: 1116 1623
PvuII CAG'CTG
Cuts at: 0 1111 2739
Size: 1111 1628
SapI GCTCTTCn'nnn_
Cuts at: 0 1463 2739
Size: 1463 1276
SexAI A'CCwGG_T
Cuts at: 0 333 2739
Size: 333 2406
SfcI C'TryA_G
Cuts at: 0 189 599 1112 1569 2338 2739
Size: 189 410 513 457 769 401
Fragments arranged by size:
769 513 457 410 401 189
SmlI C'TyrA_G
Cuts at: 0 1366 1474 1680 2739
Size: 1366 108 206 1059
Fragments arranged by size:
1366 1059 206 108
SspI AAT'ATT
Cuts at: 0 165 1630 2739
Size: 165 1465 1109
Fragments arranged by size:
1465 1109 165
StyI C'CwwG_G
Cuts at: 0 617 985 1125 1884 2429 2739
Size: 617 368 140 759 545 310
Fragments arranged by size:
759 617 545 368 310 140
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2308 2739
Size: 2308 431
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 720 752 1492 1524 2739
Size: 720 32 740 32 1215
Fragments arranged by size:
1215 740 720 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1788 2739
Size: 1788 951
XmnI GAAnn'nnTTC
Cuts at: 0 286 863 1893 2739
Size: 286 577 1030 846
Fragments arranged by size:
1030 846 577 286
Enzymes that do cut:
AarI AccI AcuI AlfI AlwNI ApaLI ApoI BanI
BbsI BbvCI BcgI BciVI BfrBI BglII Bme1580I BmrI
BpmI Bpu10I BpuEI BsaI BsaAI BseRI BseYI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrDI BsrFI BstAPI BstBI
BstXI BstYI BstZ17I BtgI BtsI DraI DraIII DrdI
EarI Eco57MI EcoNI EcoRI EcoRV FalI Hin4I HincII
HindIII MfeI MmeI MslI MspA1I NcoI NsiI NspI
PasI PflMI PfoI PshAI PstI PvuII SapI SexAI
SfcI SmlI SspI StyI TaqII TstI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AloI ApaI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BanII BbeI BclI BglI BlpI BmgBI
BmtI BplI BsaBI BsaHI BsaWI BsaXI BsgI BsiEI
BsiWI BsmBI BspEI BsrBI BsrGI BssHII BssSI BstEII
Bsu36I BtgZI ClaI CspCI EaeI EagI EciI EcoICRI
EcoO109I FseI FspI FspAI HaeII HpaI KasI KpnI
MluI MscI NaeI NarI NdeI NgoMIV NheI NotI
NruI PacI PciI PmeI PmlI PpiI PpuMI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI StuI SwaI TatI Tth111I
XbaI XhoI XmaI ZraI