Restriction map for
pFN21AE3321
(Linear) (Six-base) MAPSORT of: KIEE3321.seq Check: 5016 from: 1 to: 2712
KIEE3321.orf
With 182 enzymes: SgfI *
March 22, 2012 16:29 ..
Acc65I G'GTAC_C
Cuts at: 0 132 974 2712
Size: 132 842 1738
Fragments arranged by size:
1738 842 132
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 1834 2712
Size: 139 1695 878
Fragments arranged by size:
1695 878 139
AfeI AGC'GCT
Cuts at: 0 554 2712
Size: 554 2158
AflII C'TTAA_G
Cuts at: 0 1149 2712
Size: 1149 1563
AflIII A'CryG_T
Cuts at: 0 1571 1948 2712
Size: 1571 377 764
Fragments arranged by size:
1571 764 377
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 485 517 2712
Size: 485 32 2195
Fragments arranged by size:
2195 485 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1810 2712
Size: 1810 902
ApaLI G'TGCA_C
Cuts at: 0 81 2712
Size: 81 2631
ApoI r'AATT_y
Cuts at: 0 369 441 545 981 1598 2353 2712
Size: 369 72 104 436 617 755 359
Fragments arranged by size:
755 617 436 369 359 104 72
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 116 149 1187 1220 2712
Size: 116 33 1038 33 1492
Fragments arranged by size:
1492 1038 116 33 33
BamHI G'GATC_C
Cuts at: 0 2692 2712
Size: 2692 20
BanI G'GyrC_C
Cuts at: 0 132 974 2659 2712
Size: 132 842 1685 53
Fragments arranged by size:
1685 842 132 53
BbsI GAAGACnn'nnnn_
Cuts at: 0 1734 2345 2712
Size: 1734 611 367
Fragments arranged by size:
1734 611 367
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 862 896 2712
Size: 862 34 1816
Fragments arranged by size:
1816 862 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2143 2712
Size: 2143 569
BclI T'GATC_A
Cuts at: 0 2120 2712
Size: 2120 592
BglII A'GATC_T
Cuts at: 0 456 2712
Size: 456 2256
Bme1580I G_kGCm'C
Cuts at: 0 85 2100 2712
Size: 85 2015 612
Fragments arranged by size:
2015 612 85
BmrI ACTGGGnnnn_n'
Cuts at: 0 1726 2053 2712
Size: 1726 327 659
Fragments arranged by size:
1726 659 327
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 289 301 623 2202 2712
Size: 289 12 322 1579 510
Fragments arranged by size:
1579 510 322 289 12
Bpu10I CC'TnA_GC
Cuts at: 0 2006 2712
Size: 2006 706
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1606 2575 2712
Size: 1606 969 137
Fragments arranged by size:
1606 969 137
BsaI GGTCTCn'nnnn_
Cuts at: 0 1981 2712
Size: 1981 731
BsaAI yAC'GTr
Cuts at: 0 1949 2712
Size: 1949 763
BsaBI GATnn'nnATC
Cuts at: 0 2146 2712
Size: 2146 566
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1442 1472 2712
Size: 1442 30 1240
Fragments arranged by size:
1442 1240 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2572 2712
Size: 2572 140
BseYI C'CCAG_C
Cuts at: 0 696 703 2712
Size: 696 7 2009
Fragments arranged by size:
2009 696 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 348 2712
Size: 348 2364
BsiHKAI G_wGCw'C
Cuts at: 0 85 1076 2712
Size: 85 991 1636
Fragments arranged by size:
1636 991 85
BsmI GAATG_Cn'
Cuts at: 0 746 2712
Size: 746 1966
Bsp1286I G_dGCh'C
Cuts at: 0 85 1076 2100 2712
Size: 85 991 1024 612
Fragments arranged by size:
1024 991 612 85
BspHI T'CATG_A
Cuts at: 0 2117 2712
Size: 2117 595
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1412 2712
Size: 1412 1300
BsrGI T'GTAC_A
Cuts at: 0 2706 2712
Size: 2706 6
BstBI TT'CG_AA
Cuts at: 0 1376 2712
Size: 1376 1336
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2544 2712
Size: 2544 168
BstYI r'GATC_y
Cuts at: 0 456 1533 1727 2692 2712
Size: 456 1077 194 965 20
Fragments arranged by size:
1077 965 456 194 20
BtsI GCAGTG_nn'
Cuts at: 0 2619 2712
Size: 2619 93
ClaI AT'CG_AT
Cuts at: 0 1772 2712
Size: 1772 940
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2008 2043 2712
Size: 2008 35 669
Fragments arranged by size:
2008 669 35
DraI TTT'AAA
Cuts at: 0 596 1793 2613 2712
Size: 596 1197 820 99
Fragments arranged by size:
1197 820 596 99
DraIII CAC_nnn'GTG
Cuts at: 0 80 2712
Size: 80 2632
EaeI y'GGCC_r
Cuts at: 0 2635 2712
Size: 2635 77
EarI CTCTTCn'nnn_
Cuts at: 0 1517 2328 2595 2712
Size: 1517 811 267 117
Fragments arranged by size:
1517 811 267 117
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 289 301 623 1834 2202 2712
Size: 139 150 12 322 1211 368 510
Fragments arranged by size:
1211 510 368 322 150 139 12
EcoO109I rG'GnC_Cy
Cuts at: 0 36 700 2712
Size: 36 664 2012
Fragments arranged by size:
2012 664 36
EcoRV GAT'ATC
Cuts at: 0 576 2189 2712
Size: 576 1613 523
Fragments arranged by size:
1613 576 523
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2704 2712
Size: 2704 8
HaeII r_GCGC'y
Cuts at: 0 556 2712
Size: 556 2156
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 598 630 1264 1296 1442 1474 2065 2097
Size: 598 32 634 32 146 32 591 32
Cuts at: 2097 2712
Size: 615
Fragments arranged by size:
634 615 598 591 146 32 32 32
32
HincII GTy'rAC
Cuts at: 0 125 2412 2712
Size: 125 2287 300
Fragments arranged by size:
2287 300 125
HpaI GTT'AAC
Cuts at: 0 2412 2712
Size: 2412 300
KpnI G_GTAC'C
Cuts at: 0 136 978 2712
Size: 136 842 1734
Fragments arranged by size:
1734 842 136
MfeI C'AATT_G
Cuts at: 0 656 2405 2712
Size: 656 1749 307
Fragments arranged by size:
1749 656 307
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 419 1184 1556 1736 2712
Size: 419 765 372 180 976
Fragments arranged by size:
976 765 419 372 180
MscI TGG'CCA
Cuts at: 0 2637 2712
Size: 2637 75
MslI CAynn'nnrTG
Cuts at: 0 160 2048 2712
Size: 160 1888 664
Fragments arranged by size:
1888 664 160
MspA1I CmG'CkG
Cuts at: 0 507 696 777 1410 2712
Size: 507 189 81 633 1302
Fragments arranged by size:
1302 633 507 189 81
NspI r_CATG'y
Cuts at: 0 1575 2712
Size: 1575 1137
PciI A'CATG_T
Cuts at: 0 1571 2712
Size: 1571 1141
PmlI CAC'GTG
Cuts at: 0 1949 2712
Size: 1949 763
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 371 403 2712
Size: 371 32 2309
Fragments arranged by size:
2309 371 32
PpuMI rG'GwC_Cy
Cuts at: 0 700 2712
Size: 700 2012
PsiI TTA'TAA
Cuts at: 0 2449 2573 2712
Size: 2449 124 139
Fragments arranged by size:
2449 139 124
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2204 2236 2712
Size: 2204 32 476
Fragments arranged by size:
2204 476 32
PstI C_TGCA'G
Cuts at: 0 112 1409 2712
Size: 112 1297 1303
Fragments arranged by size:
1303 1297 112
PvuII CAG'CTG
Cuts at: 0 507 696 777 1410 2712
Size: 507 189 81 633 1302
Fragments arranged by size:
1302 633 507 189 81
SanDI GG'GwC_CC
Cuts at: 0 700 2712
Size: 700 2012
ScaI AGT'ACT
Cuts at: 0 1304 2712
Size: 1304 1408
SfcI C'TryA_G
Cuts at: 0 108 1405 2712
Size: 108 1297 1307
Fragments arranged by size:
1307 1297 108
SmlI C'TyrA_G
Cuts at: 0 1149 1585 2554 2712
Size: 1149 436 969 158
Fragments arranged by size:
1149 969 436 158
SspI AAT'ATT
Cuts at: 0 904 1371 2712
Size: 904 467 1341
Fragments arranged by size:
1341 904 467
StyI C'CwwG_G
Cuts at: 0 1430 2712
Size: 1430 1282
TatI w'GTAC_w
Cuts at: 0 1302 2157 2211 2706 2712
Size: 1302 855 54 495 6
Fragments arranged by size:
1302 855 495 54 6
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 45 77 2712
Size: 45 32 2635
Fragments arranged by size:
2635 45 32
XbaI T'CTAG_A
Cuts at: 0 1362 2712
Size: 1362 1350
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 264 2712
Size: 264 2448
XmnI GAAnn'nnTTC
Cuts at: 0 1885 2712
Size: 1885 827
Enzymes that do cut:
Acc65I AcuI AfeI AflII AflIII AloI AlwNI ApaLI
ApoI BaeI BamHI BanI BbsI BcgI BciVI BclI
BglII Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrGI BstBI BstXI BstYI BtsI ClaI
CspCI DraI DraIII EaeI EarI Eco57MI EcoO109I EcoRV
FalI HaeII Hin4I HincII HpaI KpnI MfeI MmeI
MscI MslI MspA1I NspI PciI PmlI PpiI PpuMI
PsiI PsrI PstI PvuII SanDI ScaI SfcI SmlI
SspI StyI TatI TstI XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AgeI AhdI AleI AlfI
ApaI AscI AseI AsiSI AvaI AvrII BanII BbeI
BbvCI BfrBI BglI BlpI BmgBI BmtI BplI BsaHI
BsaWI BsiEI BsiWI BsmBI BspEI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstEII BstZ17I Bsu36I BtgI BtgZI
DrdI EagI EciI EcoICRI EcoNI EcoRI FseI FspI
FspAI HindIII KasI MluI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmeI PshAI PspOMI PspXI PvuI RsrII SacI
SacII SalI SapI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI
TaqII Tth111I XhoI XmaI ZraI