Restriction map for
pFN21AE3317
(Linear) (Six-base) MAPSORT of: KIEE3317.seq Check: 2203 from: 1 to: 2637
KIEE3317.orf
With 182 enzymes: SgfI *
January 29, 2014 11:45 ..
AccI GT'mk_AC
Cuts at: 0 317 2276 2637
Size: 317 1959 361
Fragments arranged by size:
1959 361 317
AclI AA'CG_TT
Cuts at: 0 1933 2637
Size: 1933 704
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 434 1322 2487 2637
Size: 434 888 1165 150
Fragments arranged by size:
1165 888 434 150
AflII C'TTAA_G
Cuts at: 0 1471 1644 2637
Size: 1471 173 993
Fragments arranged by size:
1471 993 173
AlwNI CAG_nnn'CTG
Cuts at: 0 2613 2637
Size: 2613 24
ApaI G_GGCC'C
Cuts at: 0 1006 2262 2637
Size: 1006 1256 375
Fragments arranged by size:
1256 1006 375
ApaLI G'TGCA_C
Cuts at: 0 1483 2637
Size: 1483 1154
ApoI r'AATT_y
Cuts at: 0 100 1335 1724 2210 2308 2321 2637
Size: 100 1235 389 486 98 13 316
Fragments arranged by size:
1235 486 389 316 100 98 13
AseI AT'TA_AT
Cuts at: 0 557 1985 2637
Size: 557 1428 652
Fragments arranged by size:
1428 652 557
BamHI G'GATC_C
Cuts at: 0 3 2637
Size: 3 2634
BanI G'GyrC_C
Cuts at: 0 130 2637
Size: 130 2507
BanII G_rGCy'C
Cuts at: 0 1006 2262 2637
Size: 1006 1256 375
Fragments arranged by size:
1256 1006 375
BbeI G_GCGC'C
Cuts at: 0 134 2637
Size: 134 2503
BbsI GAAGACnn'nnnn_
Cuts at: 0 1619 2637
Size: 1619 1018
BbvCI CC'TCA_GC
Cuts at: 0 1854 2637
Size: 1854 783
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1858 1892 2637
Size: 1858 34 745
Fragments arranged by size:
1858 745 34
BclI T'GATC_A
Cuts at: 0 206 2637
Size: 206 2431
BfrBI ATG'CAT
Cuts at: 0 1990 2637
Size: 1990 647
BlpI GC'TnA_GC
Cuts at: 0 2606 2637
Size: 2606 31
Bme1580I G_kGCm'C
Cuts at: 0 1006 1131 1487 2262 2289 2637
Size: 1006 125 356 775 27 348
Fragments arranged by size:
1006 775 356 348 125 27
BmrI ACTGGGnnnn_n'
Cuts at: 0 2526 2637
Size: 2526 111
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 53 856 1114 1338 1939 2637
Size: 53 803 258 224 601 698
Fragments arranged by size:
803 698 601 258 224 53
Bpu10I CC'TnA_GC
Cuts at: 0 1854 2240 2637
Size: 1854 386 397
Fragments arranged by size:
1854 397 386
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 722 2637
Size: 722 1915
BsaBI GATnn'nnATC
Cuts at: 0 568 2637
Size: 568 2069
BsaHI Gr'CG_yC
Cuts at: 0 131 2637
Size: 131 2506
BsaWI w'CCGG_w
Cuts at: 0 474 2637
Size: 474 2163
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 50 67 1904 2637
Size: 50 17 1837 733
Fragments arranged by size:
1837 733 50 17
BseYI C'CCAG_C
Cuts at: 0 2555 2637
Size: 2555 82
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1483 2450 2637
Size: 1483 967 187
Fragments arranged by size:
1483 967 187
BsiHKAI G_wGCw'C
Cuts at: 0 1310 1487 2637
Size: 1310 177 1150
Fragments arranged by size:
1310 1150 177
BsmI GAATG_Cn'
Cuts at: 0 845 952 1029 2637
Size: 845 107 77 1608
Fragments arranged by size:
1608 845 107 77
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1060 2637
Size: 1060 1577
Bsp1286I G_dGCh'C
Cuts at: 0 1006 1131 1310 1487 2262 2289 2637
Size: 1006 125 179 177 775 27 348
Fragments arranged by size:
1006 775 348 179 177 125 27
BsrDI GCAATG_nn'
Cuts at: 0 2045 2637
Size: 2045 592
BsrGI T'GTAC_A
Cuts at: 0 1881 2637
Size: 1881 756
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1051 2637
Size: 1051 1586
BstBI TT'CG_AA
Cuts at: 0 1965 2637
Size: 1965 672
BstYI r'GATC_y
Cuts at: 0 3 1089 1815 2104 2637
Size: 3 1086 726 289 533
Fragments arranged by size:
1086 726 533 289 3
BtgI C'CryG_G
Cuts at: 0 11 2075 2262 2637
Size: 11 2064 187 375
Fragments arranged by size:
2064 375 187 11
BtsI GCAGTG_nn'
Cuts at: 0 80 295 1131 2637
Size: 80 215 836 1506
Fragments arranged by size:
1506 836 215 80
DraI TTT'AAA
Cuts at: 0 1328 2637
Size: 1328 1309
EaeI y'GGCC_r
Cuts at: 0 890 2389 2637
Size: 890 1499 248
Fragments arranged by size:
1499 890 248
EarI CTCTTCn'nnn_
Cuts at: 0 383 1298 2164 2175 2506 2637
Size: 383 915 866 11 331 131
Fragments arranged by size:
915 866 383 331 131 11
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 53 434 856 1114 1322 1338 1939 2487
Size: 53 381 422 258 208 16 601 548
Cuts at: 2487 2637
Size: 150
Fragments arranged by size:
601 548 422 381 258 208 150 53
16
EcoNI CCTnn'n_nnAGG
Cuts at: 0 35 2637
Size: 35 2602
EcoO109I rG'GnC_Cy
Cuts at: 0 1467 1909 2258 2637
Size: 1467 442 349 379
Fragments arranged by size:
1467 442 379 349
EcoRI G'AATT_C
Cuts at: 0 100 2210 2321 2637
Size: 100 2110 111 316
Fragments arranged by size:
2110 316 111 100
EcoRV GAT'ATC
Cuts at: 0 570 1298 2382 2637
Size: 570 728 1084 255
Fragments arranged by size:
1084 728 570 255
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1327 1837 2637
Size: 1327 510 800
Fragments arranged by size:
1327 800 510
FspI TGC'GCA
Cuts at: 0 1044 2637
Size: 1044 1593
HaeII r_GCGC'y
Cuts at: 0 134 2637
Size: 134 2503
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 340 372 437 469 2118 2150 2637
Size: 340 32 65 32 1649 32 487
Fragments arranged by size:
1649 487 340 65 32 32 32
HincII GTy'rAC
Cuts at: 0 1350 1495 2637
Size: 1350 145 1142
Fragments arranged by size:
1350 1142 145
KasI G'GCGC_C
Cuts at: 0 130 2637
Size: 130 2507
MfeI C'AATT_G
Cuts at: 0 668 2637
Size: 668 1969
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 854 1000 2637
Size: 854 146 1637
Fragments arranged by size:
1637 854 146
MscI TGG'CCA
Cuts at: 0 892 2391 2637
Size: 892 1499 246
Fragments arranged by size:
1499 892 246
MslI CAynn'nnrTG
Cuts at: 0 371 446 1985 2637
Size: 371 75 1539 652
Fragments arranged by size:
1539 652 371 75
MspA1I CmG'CkG
Cuts at: 0 13 2637
Size: 13 2624
NarI GG'CG_CC
Cuts at: 0 131 2637
Size: 131 2506
NcoI C'CATG_G
Cuts at: 0 2075 2262 2637
Size: 2075 187 375
Fragments arranged by size:
2075 375 187
NsiI A_TGCA'T
Cuts at: 0 1992 2637
Size: 1992 645
PfoI T'CCnGG_A
Cuts at: 0 1719 2637
Size: 1719 918
PpuMI rG'GwC_Cy
Cuts at: 0 1467 1909 2637
Size: 1467 442 728
Fragments arranged by size:
1467 728 442
PshAI GACnn'nnGTC
Cuts at: 0 1244 1355 2637
Size: 1244 111 1282
Fragments arranged by size:
1282 1244 111
PspOMI G'GGCC_C
Cuts at: 0 1002 2258 2637
Size: 1002 1256 379
Fragments arranged by size:
1256 1002 379
PstI C_TGCA'G
Cuts at: 0 302 2531 2637
Size: 302 2229 106
Fragments arranged by size:
2229 302 106
SacII CC_GC'GG
Cuts at: 0 14 2637
Size: 14 2623
SapI GCTCTTCn'nnn_
Cuts at: 0 1298 2637
Size: 1298 1339
SfcI C'TryA_G
Cuts at: 0 298 761 2058 2527 2637
Size: 298 463 1297 469 110
Fragments arranged by size:
1297 469 463 298 110
SfoI GGC'GCC
Cuts at: 0 132 2637
Size: 132 2505
SmlI C'TyrA_G
Cuts at: 0 737 1471 1644 2637
Size: 737 734 173 993
Fragments arranged by size:
993 737 734 173
SspI AAT'ATT
Cuts at: 0 1692 1809 2367 2637
Size: 1692 117 558 270
Fragments arranged by size:
1692 558 270 117
StyI C'CwwG_G
Cuts at: 0 1311 2075 2262 2386 2637
Size: 1311 764 187 124 251
Fragments arranged by size:
1311 764 251 187 124
TatI w'GTAC_w
Cuts at: 0 1881 2637
Size: 1881 756
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 830 862 2035 2067 2637
Size: 830 32 1173 32 570
Fragments arranged by size:
1173 830 570 32 32
XbaI T'CTAG_A
Cuts at: 0 429 2637
Size: 429 2208
XmnI GAAnn'nnTTC
Cuts at: 0 1075 2637
Size: 1075 1562
Enzymes that do cut:
AccI AclI AcuI AflII AlwNI ApaI ApaLI ApoI
AseI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BclI BfrBI BlpI Bme1580I BmrI BpmI Bpu10I BpuEI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiHKAI BsmI
BsmBI Bsp1286I BsrDI BsrGI BstAPI BstBI BstYI BtgI
BtsI DraI EaeI EarI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII KasI MfeI
MmeI MscI MslI MspA1I NarI NcoI NsiI PfoI
PpuMI PshAI PspOMI PstI SacII SapI SfcI SfoI
SmlI SspI StyI TatI TstI XbaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AflIII AgeI AhdI AleI
AlfI AloI AscI AsiSI AvaI AvrII BaeI BciVI
BglI BglII BmgBI BmtI BplI BsaI BsaAI BsaXI
BsiEI BsiWI BspEI BspHI BspMI BsrBI BsrFI BssHII
BssSI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI
DraIII DrdI EagI EciI EcoICRI FseI FspAI HindIII
HpaI KpnI MluI NaeI NdeI NgoMIV NheI NotI
NruI NspI PacI PasI PciI PflMI PmeI PmlI
PpiI PsiI PspXI PsrI PvuI PvuII RsrII SacI
SalI SanDI SbfI ScaI SexAI SfiI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI StuI SwaI TaqII
Tth111I XcmI XhoI XmaI ZraI