Restriction map for
pFN21AE3315
(Linear) (Six-base) MAPSORT of: KIEE3315.seq Check: 9033 from: 1 to: 2583
KIEE3315.orf
With 182 enzymes: SgfI *
July 3, 2012 17:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2130 2583
Size: 2130 453
Acc65I G'GTAC_C
Cuts at: 0 247 456 1275 2175 2583
Size: 247 209 819 900 408
Fragments arranged by size:
900 819 408 247 209
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 979 1223 1290 2188 2583
Size: 979 244 67 898 395
Fragments arranged by size:
979 898 395 244 67
AfeI AGC'GCT
Cuts at: 0 116 2583
Size: 116 2467
AflIII A'CryG_T
Cuts at: 0 770 1751 2583
Size: 770 981 832
Fragments arranged by size:
981 832 770
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2407 2583
Size: 2407 176
AlwNI CAG_nnn'CTG
Cuts at: 0 48 607 1377 2583
Size: 48 559 770 1206
Fragments arranged by size:
1206 770 559 48
ApaI G_GGCC'C
Cuts at: 0 263 2583
Size: 263 2320
ApoI r'AATT_y
Cuts at: 0 1591 2583
Size: 1591 992
AvaI C'yCGr_G
Cuts at: 0 15 1466 1745 2452 2583
Size: 15 1451 279 707 131
Fragments arranged by size:
1451 707 279 131 15
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1673 1706 2583
Size: 1673 33 877
Fragments arranged by size:
1673 877 33
BamHI G'GATC_C
Cuts at: 0 2235 2583
Size: 2235 348
BanI G'GyrC_C
Cuts at: 0 226 247 301 456 862 1192 1275 1300
Size: 226 21 54 155 406 330 83 25
Cuts at: 1300 2175 2583
Size: 875 408
Fragments arranged by size:
875 408 406 330 226 155 83 54
25 21
BanII G_rGCy'C
Cuts at: 0 263 293 350 450 2572 2583
Size: 263 30 57 100 2122 11
Fragments arranged by size:
2122 263 100 57 30 11
BbeI G_GCGC'C
Cuts at: 0 230 305 2583
Size: 230 75 2278
Fragments arranged by size:
2278 230 75
BbsI GAAGACnn'nnnn_
Cuts at: 0 1485 2450 2583
Size: 1485 965 133
Fragments arranged by size:
1485 965 133
BbvCI CC'TCA_GC
Cuts at: 0 581 2583
Size: 581 2002
BciVI GTATCCnnnnn_n'
Cuts at: 0 888 1674 2583
Size: 888 786 909
Fragments arranged by size:
909 888 786
BglI GCCn_nnn'nGGC
Cuts at: 0 1190 2583
Size: 1190 1393
BlpI GC'TnA_GC
Cuts at: 0 1674 2583
Size: 1674 909
Bme1580I G_kGCm'C
Cuts at: 0 263 865 1303 1669 1694 2583
Size: 263 602 438 366 25 889
Fragments arranged by size:
889 602 438 366 263 25
BmgBI CAC'GTC
Cuts at: 0 975 2583
Size: 975 1608
BmrI ACTGGGnnnn_n'
Cuts at: 0 816 819 846 1306 2583
Size: 816 3 27 460 1277
Fragments arranged by size:
1277 816 460 27 3
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 157 1243 2583
Size: 157 1086 1340
Fragments arranged by size:
1340 1086 157
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 466 1036 1920 2454 2583
Size: 49 417 570 884 534 129
Fragments arranged by size:
884 570 534 417 129 49
Bpu10I CC'TnA_GC
Cuts at: 0 526 581 2583
Size: 526 55 2002
Fragments arranged by size:
2002 526 55
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1501 2583
Size: 1501 1082
BsaI GGTCTCn'nnnn_
Cuts at: 0 570 982 1463 2428 2583
Size: 570 412 481 965 155
Fragments arranged by size:
965 570 481 412 155
BsaBI GATnn'nnATC
Cuts at: 0 1054 2407 2583
Size: 1054 1353 176
Fragments arranged by size:
1353 1054 176
BsaHI Gr'CG_yC
Cuts at: 0 227 302 2583
Size: 227 75 2281
Fragments arranged by size:
2281 227 75
BsaWI w'CCGG_w
Cuts at: 0 2351 2583
Size: 2351 232
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 733 763 2583
Size: 733 30 1820
Fragments arranged by size:
1820 733 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 340 775 1903 2140 2530 2583
Size: 340 435 1128 237 390 53
Fragments arranged by size:
1128 435 390 340 237 53
BseYI C'CCAG_C
Cuts at: 0 48 162 237 1402 1914 2424 2583
Size: 48 114 75 1165 512 510 159
Fragments arranged by size:
1165 512 510 159 114 75 48
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1249 2078 2329 2583
Size: 1249 829 251 254
Fragments arranged by size:
1249 829 254 251
BsiEI CG_ry'CG
Cuts at: 0 103 520 2583
Size: 103 417 2063
Fragments arranged by size:
2063 417 103
BsiHKAI G_wGCw'C
Cuts at: 0 143 293 386 527 2583
Size: 143 150 93 141 2056
Fragments arranged by size:
2056 150 143 141 93
BsmI GAATG_Cn'
Cuts at: 0 1146 1491 2583
Size: 1146 345 1092
Fragments arranged by size:
1146 1092 345
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1096 1966 2583
Size: 1096 870 617
Fragments arranged by size:
1096 870 617
Bsp1286I G_dGCh'C
Cuts at: 0 143 263 293 350 386 450 527 865
Size: 143 120 30 57 36 64 77 338
Cuts at: 865 1303 1669 1694 2572 2583
Size: 438 366 25 878 11
Fragments arranged by size:
878 438 366 338 143 120 77 64
57 36 30 25 11
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1287 2130 2149 2583
Size: 1287 843 19 434
Fragments arranged by size:
1287 843 434 19
BsrDI GCAATG_nn'
Cuts at: 0 342 798 1734 2583
Size: 342 456 936 849
Fragments arranged by size:
936 849 456 342
BsrFI r'CCGG_y
Cuts at: 0 189 250 519 585 2583
Size: 189 61 269 66 1998
Fragments arranged by size:
1998 269 189 66 61
BsrGI T'GTAC_A
Cuts at: 0 609 2583
Size: 609 1974
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1624 1729 2583
Size: 1624 105 854
Fragments arranged by size:
1624 854 105
BstYI r'GATC_y
Cuts at: 0 1049 1247 2235 2402 2583
Size: 1049 198 988 167 181
Fragments arranged by size:
1049 988 198 181 167
BtgI C'CryG_G
Cuts at: 0 317 342 1442 2583
Size: 317 25 1100 1141
Fragments arranged by size:
1141 1100 317 25
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 547 830 2583
Size: 547 283 1753
Fragments arranged by size:
1753 547 283
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 908 943 2583
Size: 908 35 1640
Fragments arranged by size:
1640 908 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 1027 2583
Size: 1027 1556
EaeI y'GGCC_r
Cuts at: 0 100 339 517 1354 2549 2583
Size: 100 239 178 837 1195 34
Fragments arranged by size:
1195 837 239 178 100 34
EagI C'GGCC_G
Cuts at: 0 100 517 2583
Size: 100 417 2066
Fragments arranged by size:
2066 417 100
EarI CTCTTCn'nnn_
Cuts at: 0 1652 2485 2583
Size: 1652 833 98
Fragments arranged by size:
1652 833 98
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 451 458 2583
Size: 451 7 2125
Fragments arranged by size:
2125 451 7
EcoICRI GAG'CTC
Cuts at: 0 291 2583
Size: 291 2292
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 466 979 1036 1223 1290 1920 2188
Size: 49 417 513 57 187 67 630 268
Cuts at: 2188 2454 2583
Size: 266 129
Fragments arranged by size:
630 513 417 268 266 187 129 67
57 49
EcoNI CCTnn'n_nnAGG
Cuts at: 0 691 2583
Size: 691 1892
EcoO109I rG'GnC_Cy
Cuts at: 0 7 159 207 260 660 821 2583
Size: 7 152 48 53 400 161 1762
Fragments arranged by size:
1762 400 161 152 53 48 7
EcoRV GAT'ATC
Cuts at: 0 1177 2583
Size: 1177 1406
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1422 2583
Size: 1422 1161
FspI TGC'GCA
Cuts at: 0 313 2263 2583
Size: 313 1950 320
Fragments arranged by size:
1950 320 313
FspAI rTGC'GCAy
Cuts at: 0 313 2583
Size: 313 2270
HaeII r_GCGC'y
Cuts at: 0 118 230 305 2583
Size: 118 112 75 2278
Fragments arranged by size:
2278 118 112 75
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 733 765 2583
Size: 733 32 1818
Fragments arranged by size:
1818 733 32
HindIII A'AGCT_T
Cuts at: 0 148 2583
Size: 148 2435
KasI G'GCGC_C
Cuts at: 0 226 301 2583
Size: 226 75 2282
Fragments arranged by size:
2282 226 75
KpnI G_GTAC'C
Cuts at: 0 251 460 1279 2179 2583
Size: 251 209 819 900 404
Fragments arranged by size:
900 819 404 251 209
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2124 2195 2583
Size: 2124 71 388
Fragments arranged by size:
2124 388 71
MscI TGG'CCA
Cuts at: 0 341 2551 2583
Size: 341 2210 32
Fragments arranged by size:
2210 341 32
MslI CAynn'nnrTG
Cuts at: 0 2068 2563 2583
Size: 2068 495 20
Fragments arranged by size:
2068 495 20
MspA1I CmG'CkG
Cuts at: 0 45 105 166 375 486 1125 1353 1678
Size: 45 60 61 209 111 639 228 325
Cuts at: 1678 1809 1918 2583
Size: 131 109 665
Fragments arranged by size:
665 639 325 228 209 131 111 109
61 60 45
NaeI GCC'GGC
Cuts at: 0 587 2583
Size: 587 1996
NarI GG'CG_CC
Cuts at: 0 227 302 2583
Size: 227 75 2281
Fragments arranged by size:
2281 227 75
NcoI C'CATG_G
Cuts at: 0 1442 2583
Size: 1442 1141
NgoMIV G'CCGG_C
Cuts at: 0 585 2583
Size: 585 1998
NotI GC'GGCC_GC
Cuts at: 0 100 2583
Size: 100 2483
NspI r_CATG'y
Cuts at: 0 1755 2583
Size: 1755 828
PasI CC'CwG_GG
Cuts at: 0 2050 2583
Size: 2050 533
PciI A'CATG_T
Cuts at: 0 1751 2583
Size: 1751 832
PflMI CCAn_nnn'nTGG
Cuts at: 0 1296 2034 2583
Size: 1296 738 549
Fragments arranged by size:
1296 738 549
PfoI T'CCnGG_A
Cuts at: 0 1013 1631 1943 2583
Size: 1013 618 312 640
Fragments arranged by size:
1013 640 618 312
PpuMI rG'GwC_Cy
Cuts at: 0 7 159 660 821 2583
Size: 7 152 501 161 1762
Fragments arranged by size:
1762 501 161 152 7
PspOMI G'GGCC_C
Cuts at: 0 259 2583
Size: 259 2324
PstI C_TGCA'G
Cuts at: 0 407 1235 2583
Size: 407 828 1348
Fragments arranged by size:
1348 828 407
PvuII CAG'CTG
Cuts at: 0 45 375 1809 1918 2583
Size: 45 330 1434 109 665
Fragments arranged by size:
1434 665 330 109 45
SacI G_AGCT'C
Cuts at: 0 293 2583
Size: 293 2290
SanDI GG'GwC_CC
Cuts at: 0 821 2583
Size: 821 1762
SapI GCTCTTCn'nnn_
Cuts at: 0 1652 2583
Size: 1652 931
ScaI AGT'ACT
Cuts at: 0 1208 2583
Size: 1208 1375
SexAI A'CCwGG_T
Cuts at: 0 1271 2583
Size: 1271 1312
SfcI C'TryA_G
Cuts at: 0 403 1231 1551 2583
Size: 403 828 320 1032
Fragments arranged by size:
1032 828 403 320
SfoI GGC'GCC
Cuts at: 0 228 303 2583
Size: 228 75 2280
Fragments arranged by size:
2280 228 75
SmaI CCC'GGG
Cuts at: 0 17 2583
Size: 17 2566
SmlI C'TyrA_G
Cuts at: 0 1466 1516 2583
Size: 1466 50 1067
Fragments arranged by size:
1466 1067 50
SpeI A'CTAG_T
Cuts at: 0 1162 2583
Size: 1162 1421
SrfI GCCC'GGGC
Cuts at: 0 17 2583
Size: 17 2566
StuI AGG'CCT
Cuts at: 0 1361 1514 2583
Size: 1361 153 1069
Fragments arranged by size:
1361 1069 153
StyI C'CwwG_G
Cuts at: 0 1442 1617 2504 2572 2583
Size: 1442 175 887 68 11
Fragments arranged by size:
1442 887 175 68 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 342 1513 2583
Size: 342 1171 1070
Fragments arranged by size:
1171 1070 342
TatI w'GTAC_w
Cuts at: 0 609 1206 2016 2583
Size: 609 597 810 567
Fragments arranged by size:
810 609 597 567
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1540 1572 2583
Size: 1540 32 1011
Fragments arranged by size:
1540 1011 32
XbaI T'CTAG_A
Cuts at: 0 1117 2583
Size: 1117 1466
XhoI C'TCGA_G
Cuts at: 0 1466 2583
Size: 1466 1117
XmaI C'CCGG_G
Cuts at: 0 15 2583
Size: 15 2568
XmnI GAAnn'nnTTC
Cuts at: 0 1330 2335 2583
Size: 1330 1005 248
Fragments arranged by size:
1330 1005 248
Enzymes that do cut:
AarI Acc65I AcuI AfeI AflIII AlfI AlwNI ApaI
ApoI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BciVI BglI BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspMI BsrDI BsrFI BsrGI BstXI BstYI BtgI BtgZI
CspCI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRV FalI FspI FspAI HaeII Hin4I
HindIII KasI KpnI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NotI NspI PasI PciI PflMI
PfoI PpuMI PspOMI PstI PvuII SacI SanDI SapI
ScaI SexAI SfcI SfoI SmaI SmlI SpeI SrfI
StuI StyI TaqII TatI TstI XbaI XhoI XmaI
XmnI
Enzymes that do not cut:
AatII AccI AclI AflII AgeI AhdI AleI AloI
ApaLI AscI AseI AsiSI AvrII BcgI BclI BfrBI
BglII BmtI BsaAI BsiWI BspEI BspHI BsrBI BssHII
BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtsI ClaI
DraI DraIII EcoRI FseI HincII HpaI MfeI MluI
NdeI NheI NruI NsiI PacI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI
SbfI SfiI SgfI SgrAI SnaBI SphI SspI SwaI
Tth111I XcmI ZraI