Restriction map for
pFN21AE3314
(Linear) (Six-base) MAPSORT of: KIEE3314.seq Check: 5127 from: 1 to: 2565
KIEE3314.orf
With 182 enzymes: SgfI *
January 29, 2014 11:45 ..
AatII G_ACGT'C
Cuts at: 0 980 2565
Size: 980 1585
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 268 944 1142 2080 2565
Size: 268 676 198 938 485
Fragments arranged by size:
938 676 485 268 198
AflII C'TTAA_G
Cuts at: 0 1867 2565
Size: 1867 698
AflIII A'CryG_T
Cuts at: 0 746 869 2565
Size: 746 123 1696
Fragments arranged by size:
1696 746 123
AleI CACnn'nnGTG
Cuts at: 0 295 2565
Size: 295 2270
AlwNI CAG_nnn'CTG
Cuts at: 0 420 1102 1570 1621 2492 2565
Size: 420 682 468 51 871 73
Fragments arranged by size:
871 682 468 420 73 51
ApoI r'AATT_y
Cuts at: 0 820 837 1477 1759 2565
Size: 820 17 640 282 806
Fragments arranged by size:
820 806 640 282 17
BamHI G'GATC_C
Cuts at: 0 1196 2225 2565
Size: 1196 1029 340
Fragments arranged by size:
1196 1029 340
BanI G'GyrC_C
Cuts at: 0 215 311 1005 1146 1551 2565
Size: 215 96 694 141 405 1014
Fragments arranged by size:
1014 694 405 215 141 96
BanII G_rGCy'C
Cuts at: 0 2012 2565
Size: 2012 553
BbeI G_GCGC'C
Cuts at: 0 315 2565
Size: 315 2250
BciVI GTATCCnnnnn_n'
Cuts at: 0 658 2565
Size: 658 1907
BclI T'GATC_A
Cuts at: 0 1955 2565
Size: 1955 610
BglI GCCn_nnn'nGGC
Cuts at: 0 1455 2565
Size: 1455 1110
BglII A'GATC_T
Cuts at: 0 890 1980 2127 2565
Size: 890 1090 147 438
Fragments arranged by size:
1090 890 438 147
BlpI GC'TnA_GC
Cuts at: 0 2485 2565
Size: 2485 80
Bme1580I G_kGCm'C
Cuts at: 0 220 1556 2565
Size: 220 1336 1009
Fragments arranged by size:
1336 1009 220
BmrI ACTGGGnnnn_n'
Cuts at: 0 980 1529 2565
Size: 980 549 1036
Fragments arranged by size:
1036 980 549
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 572 635 1281 1306 1469 2010 2423 2514
Size: 572 63 646 25 163 541 413 91
Cuts at: 2514 2565
Size: 51
Fragments arranged by size:
646 572 541 413 163 91 63 51
25
Bpu10I CC'TnA_GC
Cuts at: 0 68 2565
Size: 68 2497
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 72 1879 2565
Size: 72 1807 686
Fragments arranged by size:
1807 686 72
BsaI GGTCTCn'nnnn_
Cuts at: 0 2411 2565
Size: 2411 154
BsaAI yAC'GTr
Cuts at: 0 747 2565
Size: 747 1818
BsaBI GATnn'nnATC
Cuts at: 0 1879 1985 2565
Size: 1879 106 580
Fragments arranged by size:
1879 580 106
BsaHI Gr'CG_yC
Cuts at: 0 312 977 2565
Size: 312 665 1588
Fragments arranged by size:
1588 665 312
BsaWI w'CCGG_w
Cuts at: 0 491 2222 2565
Size: 491 1731 343
Fragments arranged by size:
1731 491 343
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 892 922 1454 1483 1484 1513 2565
Size: 892 30 532 29 1 29 1052
Fragments arranged by size:
1052 892 532 30 29 29 1
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1365 1507 1510 1681 2565
Size: 1365 142 3 171 884
Fragments arranged by size:
1365 884 171 142 3
BseYI C'CCAG_C
Cuts at: 0 592 1704 2280 2369 2565
Size: 592 1112 576 89 196
Fragments arranged by size:
1112 592 576 196 89
BsiEI CG_ry'CG
Cuts at: 0 1416 2565
Size: 1416 1149
BsiHKAI G_wGCw'C
Cuts at: 0 993 2246 2565
Size: 993 1253 319
Fragments arranged by size:
1253 993 319
BsmI GAATG_Cn'
Cuts at: 0 23 1854 2565
Size: 23 1831 711
Fragments arranged by size:
1831 711 23
Bsp1286I G_dGCh'C
Cuts at: 0 220 993 1556 2012 2246 2565
Size: 220 773 563 456 234 319
Fragments arranged by size:
773 563 456 319 234 220
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1646 2565
Size: 1646 919
BsrBI CCG'CTC
Cuts at: 0 920 1373 2565
Size: 920 453 1192
Fragments arranged by size:
1192 920 453
BsrDI GCAATG_nn'
Cuts at: 0 1112 2565
Size: 1112 1453
BsrGI T'GTAC_A
Cuts at: 0 350 1109 2204 2565
Size: 350 759 1095 361
Fragments arranged by size:
1095 759 361 350
BssSI C'ACGA_G
Cuts at: 0 2005 2565
Size: 2005 560
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1281 2565
Size: 1281 1284
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1537 2565
Size: 1537 1028
BstYI r'GATC_y
Cuts at: 0 386 890 1196 1980 2127 2225 2565
Size: 386 504 306 784 147 98 340
Fragments arranged by size:
784 504 386 340 306 147 98
Bsu36I CC'TnA_GG
Cuts at: 0 1544 2565
Size: 1544 1021
BtgI C'CryG_G
Cuts at: 0 1631 2565
Size: 1631 934
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2045 2565
Size: 2045 520
BtsI GCAGTG_nn'
Cuts at: 0 858 2246 2565
Size: 858 1388 319
Fragments arranged by size:
1388 858 319
DraIII CAC_nnn'GTG
Cuts at: 0 1387 2565
Size: 1387 1178
EaeI y'GGCC_r
Cuts at: 0 1025 1217 1238 2132 2565
Size: 1025 192 21 894 433
Fragments arranged by size:
1025 894 433 192 21
EarI CTCTTCn'nnn_
Cuts at: 0 2493 2565
Size: 2493 72
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 411 792 2565
Size: 411 381 1773
Fragments arranged by size:
1773 411 381
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 268 572 635 944 1142 1281 1306 1469
Size: 268 304 63 309 198 139 25 163
Cuts at: 1469 2010 2080 2423 2514 2565
Size: 541 70 343 91 51
Fragments arranged by size:
541 343 309 304 268 198 163 139
91 70 63 51 25
EcoO109I rG'GnC_Cy
Cuts at: 0 302 1899 2565
Size: 302 1597 666
Fragments arranged by size:
1597 666 302
HaeII r_GCGC'y
Cuts at: 0 315 2565
Size: 315 2250
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 608 640 691 723 1398 1430 1483 1515
Size: 608 32 51 32 675 32 53 32
Cuts at: 1515 1627 1659 1872 1904 2565
Size: 112 32 213 32 661
Fragments arranged by size:
675 661 608 213 112 53 51 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 981 2565
Size: 981 1584
KasI G'GCGC_C
Cuts at: 0 311 2565
Size: 311 2254
MfeI C'AATT_G
Cuts at: 0 691 2565
Size: 691 1874
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 642 1424 2565
Size: 642 782 1141
Fragments arranged by size:
1141 782 642
MscI TGG'CCA
Cuts at: 0 1027 1219 1240 2134 2565
Size: 1027 192 21 894 431
Fragments arranged by size:
1027 894 431 192 21
MslI CAynn'nnrTG
Cuts at: 0 295 523 847 1736 2565
Size: 295 228 324 889 829
Fragments arranged by size:
889 829 324 295 228
MspA1I CmG'CkG
Cuts at: 0 169 592 1511 2255 2373 2489 2565
Size: 169 423 919 744 118 116 76
Fragments arranged by size:
919 744 423 169 118 116 76
NarI GG'CG_CC
Cuts at: 0 312 2565
Size: 312 2253
NcoI C'CATG_G
Cuts at: 0 1631 2565
Size: 1631 934
NspI r_CATG'y
Cuts at: 0 108 873 1359 2565
Size: 108 765 486 1206
Fragments arranged by size:
1206 765 486 108
PasI CC'CwG_GG
Cuts at: 0 306 2565
Size: 306 2259
PciI A'CATG_T
Cuts at: 0 869 2565
Size: 869 1696
PflMI CCAn_nnn'nTGG
Cuts at: 0 152 935 1023 2565
Size: 152 783 88 1542
Fragments arranged by size:
1542 783 152 88
PfoI T'CCnGG_A
Cuts at: 0 968 2565
Size: 968 1597
PmlI CAC'GTG
Cuts at: 0 747 2565
Size: 747 1818
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2503 2535 2565
Size: 2503 32 30
Fragments arranged by size:
2503 32 30
PpuMI rG'GwC_Cy
Cuts at: 0 302 1899 2565
Size: 302 1597 666
Fragments arranged by size:
1597 666 302
PstI C_TGCA'G
Cuts at: 0 263 1510 2565
Size: 263 1247 1055
Fragments arranged by size:
1247 1055 263
PvuI CG_AT'CG
Cuts at: 0 1416 2565
Size: 1416 1149
PvuII CAG'CTG
Cuts at: 0 169 592 2373 2489 2565
Size: 169 423 1781 116 76
Fragments arranged by size:
1781 423 169 116 76
SanDI GG'GwC_CC
Cuts at: 0 302 2565
Size: 302 2263
SfcI C'TryA_G
Cuts at: 0 259 1506 2294 2565
Size: 259 1247 788 271
Fragments arranged by size:
1247 788 271 259
SfoI GGC'GCC
Cuts at: 0 313 2565
Size: 313 2252
SmlI C'TyrA_G
Cuts at: 0 51 1867 1894 2565
Size: 51 1816 27 671
Fragments arranged by size:
1816 671 51 27
StyI C'CwwG_G
Cuts at: 0 1587 1631 2421 2565
Size: 1587 44 790 144
Fragments arranged by size:
1587 790 144 44
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 712 742 1164 2565
Size: 712 30 422 1401
Fragments arranged by size:
1401 712 422 30
TatI w'GTAC_w
Cuts at: 0 350 606 1109 2204 2565
Size: 350 256 503 1095 361
Fragments arranged by size:
1095 503 361 350 256
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 771 803 892 924 1640 1672 2565
Size: 771 32 89 32 716 32 893
Fragments arranged by size:
893 771 716 89 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 21 697 1140 2083 2565
Size: 21 676 443 943 482
Fragments arranged by size:
943 676 482 443 21
ZraI GAC'GTC
Cuts at: 0 978 2565
Size: 978 1587
Enzymes that do cut:
AatII AcuI AflII AflIII AleI AlwNI ApoI BamHI
BanI BanII BbeI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI
Bsp1286I BspMI BsrBI BsrDI BsrGI BssSI BstAPI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraIII EaeI EarI
EciI Eco57MI EcoO109I HaeII Hin4I HincII KasI MfeI
MmeI MscI MslI MspA1I NarI NcoI NspI PasI
PciI PflMI PfoI PmlI PpiI PpuMI PstI PvuI
PvuII SanDI SfcI SfoI SmlI StyI TaqII TatI
TstI XcmI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AgeI AhdI AlfI
AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI BplI
BsgI BsiWI BsmBI BspEI BspHI BsrFI BssHII BstBI
BstEII BstZ17I ClaI CspCI DraI DrdI EagI EcoICRI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HindIII
HpaI KpnI MluI NaeI NdeI NgoMIV NheI NotI
NruI NsiI PacI PmeI PshAI PsiI PspOMI PspXI
PsrI RsrII SacI SacII SalI SapI SbfI ScaI
SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI Tth111I XbaI XhoI XmaI
XmnI