Restriction map for
pFN21AE3312
(Linear) (Six-base) MAPSORT of: KIEE3312.seq Check: 7269 from: 1 to: 2385
KIEE3312.orf
With 182 enzymes: SgfI *
July 3, 2012 17:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1029 2241 2385
Size: 1029 1212 144
Fragments arranged by size:
1212 1029 144
AccI GT'mk_AC
Cuts at: 0 2326 2385
Size: 2326 59
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 758 1736 1745 2141 2385
Size: 758 978 9 396 244
Fragments arranged by size:
978 758 396 244 9
AleI CACnn'nnGTG
Cuts at: 0 2315 2385
Size: 2315 70
AlwNI CAG_nnn'CTG
Cuts at: 0 306 2007 2176 2385
Size: 306 1701 169 209
Fragments arranged by size:
1701 306 209 169
ApoI r'AATT_y
Cuts at: 0 633 2065 2281 2359 2379 2385
Size: 633 1432 216 78 20 6
Fragments arranged by size:
1432 633 216 78 20 6
AseI AT'TA_AT
Cuts at: 0 1976 2385
Size: 1976 409
AvaI C'yCGr_G
Cuts at: 0 458 2385
Size: 458 1927
AvrII C'CTAG_G
Cuts at: 0 447 2385
Size: 447 1938
BanII G_rGCy'C
Cuts at: 0 523 573 946 2385
Size: 523 50 373 1439
Fragments arranged by size:
1439 523 373 50
BbsI GAAGACnn'nnnn_
Cuts at: 0 402 1820 2385
Size: 402 1418 565
Fragments arranged by size:
1418 565 402
BglII A'GATC_T
Cuts at: 0 390 1527 2385
Size: 390 1137 858
Fragments arranged by size:
1137 858 390
BlpI GC'TnA_GC
Cuts at: 0 724 2385
Size: 724 1661
Bme1580I G_kGCm'C
Cuts at: 0 1257 2385
Size: 1257 1128
BmrI ACTGGGnnnn_n'
Cuts at: 0 1310 1652 2050 2385
Size: 1310 342 398 335
Fragments arranged by size:
1310 398 342 335
BmtI G_CTAG'C
Cuts at: 0 491 2385
Size: 491 1894
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 908 2385
Size: 908 1477
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 268 1077 1196 2226 2385
Size: 268 809 119 1030 159
Fragments arranged by size:
1030 809 268 159 119
BsaI GGTCTCn'nnnn_
Cuts at: 0 1012 2385
Size: 1012 1373
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 98 128 2385
Size: 98 30 2257
Fragments arranged by size:
2257 98 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 236 771 1237 1494 1544 1728 1833 2284
Size: 236 535 466 257 50 184 105 451
Cuts at: 2284 2287 2385
Size: 3 98
Fragments arranged by size:
535 466 451 257 236 184 105 98
50 3
BseYI C'CCAG_C
Cuts at: 0 161 1256 2385
Size: 161 1095 1129
Fragments arranged by size:
1129 1095 161
BsiHKAI G_wGCw'C
Cuts at: 0 1499 2312 2385
Size: 1499 813 73
Fragments arranged by size:
1499 813 73
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1781 2385
Size: 1781 604
Bsp1286I G_dGCh'C
Cuts at: 0 523 573 946 1257 1499 2312 2385
Size: 523 50 373 311 242 813 73
Fragments arranged by size:
813 523 373 311 242 73 50
BspHI T'CATG_A
Cuts at: 0 906 2385
Size: 906 1479
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 32 1029 1051 1119 2241 2385
Size: 32 997 22 68 1122 144
Fragments arranged by size:
1122 997 144 68 32 22
BsrBI CCG'CTC
Cuts at: 0 138 2385
Size: 138 2247
BsrFI r'CCGG_y
Cuts at: 0 74 2385
Size: 74 2311
BsrGI T'GTAC_A
Cuts at: 0 336 2385
Size: 336 2049
BstAPI GCAn_nnn'nTGC
Cuts at: 0 306 2385
Size: 306 2079
BstYI r'GATC_y
Cuts at: 0 390 1527 2385
Size: 390 1137 858
Fragments arranged by size:
1137 858 390
BstZ17I GTA'TAC
Cuts at: 0 2327 2385
Size: 2327 58
Bsu36I CC'TnA_GG
Cuts at: 0 766 2385
Size: 766 1619
BtgI C'CryG_G
Cuts at: 0 347 2385
Size: 347 2038
BtsI GCAGTG_nn'
Cuts at: 0 1674 2385
Size: 1674 711
DraI TTT'AAA
Cuts at: 0 2145 2385
Size: 2145 240
DrdI GACnn_nn'nnGTC
Cuts at: 0 1855 2385
Size: 1855 530
EaeI y'GGCC_r
Cuts at: 0 8 596 2385
Size: 8 588 1789
Fragments arranged by size:
1789 588 8
EarI CTCTTCn'nnn_
Cuts at: 0 939 1214 1755 2117 2385
Size: 939 275 541 362 268
Fragments arranged by size:
939 541 362 275 268
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 758 908 1736 1745 2141 2385
Size: 758 150 828 9 396 244
Fragments arranged by size:
828 758 396 244 150 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1115 2385
Size: 1115 1270
EcoO109I rG'GnC_Cy
Cuts at: 0 444 998 1756 2385
Size: 444 554 758 629
Fragments arranged by size:
758 629 554 444
EcoRI G'AATT_C
Cuts at: 0 633 2065 2385
Size: 633 1432 320
Fragments arranged by size:
1432 633 320
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 319 2385
Size: 319 2066
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 569 601 844 876 883 915 1585 1617
Size: 569 32 243 32 7 32 670 32
Cuts at: 1617 1663 1695 1869 1901 2385
Size: 46 32 174 32 484
Fragments arranged by size:
670 569 484 243 174 46 32 32
32 32 32 32 7
MfeI C'AATT_G
Cuts at: 0 1990 2385
Size: 1990 395
MscI TGG'CCA
Cuts at: 0 598 2385
Size: 598 1787
MslI CAynn'nnrTG
Cuts at: 0 244 2315 2385
Size: 244 2071 70
Fragments arranged by size:
2071 244 70
NcoI C'CATG_G
Cuts at: 0 347 2385
Size: 347 2038
NheI G'CTAG_C
Cuts at: 0 487 2385
Size: 487 1898
NspI r_CATG'y
Cuts at: 0 613 2385
Size: 613 1772
PasI CC'CwG_GG
Cuts at: 0 995 2385
Size: 995 1390
PpuMI rG'GwC_Cy
Cuts at: 0 444 2385
Size: 444 1941
PstI C_TGCA'G
Cuts at: 0 1235 1334 1855 2385
Size: 1235 99 521 530
Fragments arranged by size:
1235 530 521 99
SfcI C'TryA_G
Cuts at: 0 875 1231 1330 1851 2385
Size: 875 356 99 521 534
Fragments arranged by size:
875 534 521 356 99
SgrAI Cr'CCGG_yG
Cuts at: 0 74 2385
Size: 74 2311
SmlI C'TyrA_G
Cuts at: 0 283 1056 1211 2205 2385
Size: 283 773 155 994 180
Fragments arranged by size:
994 773 283 180 155
SpeI A'CTAG_T
Cuts at: 0 2193 2385
Size: 2193 192
SphI G_CATG'C
Cuts at: 0 613 2385
Size: 613 1772
SspI AAT'ATT
Cuts at: 0 1098 2385
Size: 1098 1287
StuI AGG'CCT
Cuts at: 0 735 2385
Size: 735 1650
StyI C'CwwG_G
Cuts at: 0 347 447 1982 2385
Size: 347 100 1535 403
Fragments arranged by size:
1535 403 347 100
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 952 1430 2305 2385
Size: 952 478 875 80
Fragments arranged by size:
952 875 478 80
TatI w'GTAC_w
Cuts at: 0 336 2385
Size: 336 2049
Tth111I GACn'n_nGTC
Cuts at: 0 1294 2385
Size: 1294 1091
Enzymes that do cut:
AarI AccI AcuI AleI AlwNI ApoI AseI AvaI
AvrII BanII BbsI BglII BlpI Bme1580I BmrI BmtI
BpmI BpuEI BsaI BsaXI BseRI BseYI BsiHKAI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrFI BsrGI BstAPI BstYI
BstZ17I Bsu36I BtgI BtsI DraI DrdI EaeI EarI
Eco57MI EcoNI EcoO109I EcoRI FalI Hin4I MfeI MscI
MslI NcoI NheI NspI PasI PpuMI PstI SfcI
SgrAI SmlI SpeI SphI SspI StuI StyI TaqII
TatI Tth111I
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AlfI AloI ApaI ApaLI AscI AsiSI BaeI BamHI
BanI BbeI BbvCI BcgI BciVI BclI BfrBI BglI
BmgBI BplI Bpu10I BsaAI BsaBI BsaHI BsaWI BsgI
BsiEI BsiWI BsmI BspEI BsrDI BssHII BssSI BstBI
BstEII BstXI BtgZI ClaI CspCI DraIII EagI EciI
EcoICRI EcoRV FseI FspI FspAI HaeII HincII HindIII
HpaI KasI KpnI MluI MmeI MspA1I NaeI NarI
NdeI NgoMIV NotI NruI NsiI PacI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SmaI
SnaBI SrfI SwaI TstI XbaI XcmI XhoI XmaI
XmnI ZraI