Restriction map for
pFN21AE3308
(Linear) (Six-base) MAPSORT of: KIEE3308.seq Check: 2930 from: 1 to: 2409
KIEE3308.orf
With 182 enzymes: SgfI *
July 3, 2012 17:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1900 2409
Size: 1900 509
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 161 424 766 1130 1446 2409
Size: 37 124 263 342 364 316 963
Fragments arranged by size:
963 364 342 316 263 124 37
AfeI AGC'GCT
Cuts at: 0 853 2409
Size: 853 1556
AflIII A'CryG_T
Cuts at: 0 379 734 2409
Size: 379 355 1675
Fragments arranged by size:
1675 379 355
AgeI A'CCGG_T
Cuts at: 0 2359 2409
Size: 2359 50
AleI CACnn'nnGTG
Cuts at: 0 1291 2409
Size: 1291 1118
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2272 2409
Size: 2272 137
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1142 1174 2409
Size: 1142 32 1235
Fragments arranged by size:
1235 1142 32
AlwNI CAG_nnn'CTG
Cuts at: 0 999 1162 2139 2409
Size: 999 163 977 270
Fragments arranged by size:
999 977 270 163
ApaI G_GGCC'C
Cuts at: 0 24 2347 2409
Size: 24 2323 62
Fragments arranged by size:
2323 62 24
ApoI r'AATT_y
Cuts at: 0 40 103 1358 1369 1634 1822 1846 2409
Size: 40 63 1255 11 265 188 24 563
Fragments arranged by size:
1255 563 265 188 63 40 24 11
AvaI C'yCGr_G
Cuts at: 0 863 2409
Size: 863 1546
BamHI G'GATC_C
Cuts at: 0 2005 2409
Size: 2005 404
BanI G'GyrC_C
Cuts at: 0 340 2409
Size: 340 2069
BanII G_rGCy'C
Cuts at: 0 24 2347 2409
Size: 24 2323 62
Fragments arranged by size:
2323 62 24
BbsI GAAGACnn'nnnn_
Cuts at: 0 208 450 2409
Size: 208 242 1959
Fragments arranged by size:
1959 242 208
BbvCI CC'TCA_GC
Cuts at: 0 28 2409
Size: 28 2381
BclI T'GATC_A
Cuts at: 0 624 2409
Size: 624 1785
BfrBI ATG'CAT
Cuts at: 0 546 1998 2409
Size: 546 1452 411
Fragments arranged by size:
1452 546 411
BglII A'GATC_T
Cuts at: 0 2121 2409
Size: 2121 288
Bme1580I G_kGCm'C
Cuts at: 0 24 2347 2409
Size: 24 2323 62
Fragments arranged by size:
2323 62 24
BmrI ACTGGGnnnn_n'
Cuts at: 0 942 1720 2409
Size: 942 778 689
Fragments arranged by size:
942 778 689
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2270 2409
Size: 2270 139
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 667 1529 2161 2409
Size: 667 862 632 248
Fragments arranged by size:
862 667 632 248
Bpu10I CC'TnA_GC
Cuts at: 0 28 2409
Size: 28 2381
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 481 583 2409
Size: 481 102 1826
Fragments arranged by size:
1826 481 102
BsaI GGTCTCn'nnnn_
Cuts at: 0 1715 2409
Size: 1715 694
BsaAI yAC'GTr
Cuts at: 0 380 735 2409
Size: 380 355 1674
Fragments arranged by size:
1674 380 355
BsaWI w'CCGG_w
Cuts at: 0 2359 2409
Size: 2359 50
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 351 381 1142 1172 1712 1742 2409
Size: 351 30 761 30 540 30 667
Fragments arranged by size:
761 667 540 351 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1619 2409
Size: 1619 790
BseYI C'CCAG_C
Cuts at: 0 1205 1499 2409
Size: 1205 294 910
Fragments arranged by size:
1205 910 294
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 224 2409
Size: 224 2185
BsiHKAI G_wGCw'C
Cuts at: 0 695 870 2227 2409
Size: 695 175 1357 182
Fragments arranged by size:
1357 695 182 175
Bsp1286I G_dGCh'C
Cuts at: 0 24 695 870 2227 2347 2409
Size: 24 671 175 1357 120 62
Fragments arranged by size:
1357 671 175 120 62 24
BspHI T'CATG_A
Cuts at: 0 197 627 1799 2409
Size: 197 430 1172 610
Fragments arranged by size:
1172 610 430 197
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 771 1272 1900 2409
Size: 771 501 628 509
Fragments arranged by size:
771 628 509 501
BsrDI GCAATG_nn'
Cuts at: 0 1581 1778 2409
Size: 1581 197 631
Fragments arranged by size:
1581 631 197
BsrFI r'CCGG_y
Cuts at: 0 2207 2359 2409
Size: 2207 152 50
Fragments arranged by size:
2207 152 50
BstAPI GCAn_nnn'nTGC
Cuts at: 0 779 2409
Size: 779 1630
BstXI CCAn_nnnn'nTGG
Cuts at: 0 115 1767 2172 2409
Size: 115 1652 405 237
Fragments arranged by size:
1652 405 237 115
BstYI r'GATC_y
Cuts at: 0 2005 2121 2409
Size: 2005 116 288
Fragments arranged by size:
2005 288 116
Bsu36I CC'TnA_GG
Cuts at: 0 449 1163 2409
Size: 449 714 1246
Fragments arranged by size:
1246 714 449
BtgI C'CryG_G
Cuts at: 0 108 1493 2165 2281 2409
Size: 108 1385 672 116 128
Fragments arranged by size:
1385 672 128 116 108
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1611 2409
Size: 1611 798
BtsI GCAGTG_nn'
Cuts at: 0 236 1033 2409
Size: 236 797 1376
Fragments arranged by size:
1376 797 236
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1320 1355 2409
Size: 1320 35 1054
Fragments arranged by size:
1320 1054 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 2250 2409
Size: 2250 159
EaeI y'GGCC_r
Cuts at: 0 2205 2409
Size: 2205 204
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 161 424 667 766 1130 1446 1529
Size: 37 124 263 243 99 364 316 83
Cuts at: 1529 2161 2409
Size: 632 248
Fragments arranged by size:
632 364 316 263 248 243 124 99
83 37
EcoO109I rG'GnC_Cy
Cuts at: 0 813 996 2409
Size: 813 183 1413
Fragments arranged by size:
1413 813 183
EcoRI G'AATT_C
Cuts at: 0 103 2409
Size: 103 2306
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 175 238 2409
Size: 175 63 2171
Fragments arranged by size:
2171 175 63
HaeII r_GCGC'y
Cuts at: 0 855 2409
Size: 855 1554
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 351 383 1545 1577 1781 1813 2409
Size: 351 32 1162 32 204 32 596
Fragments arranged by size:
1162 596 351 204 32 32 32
HindIII A'AGCT_T
Cuts at: 0 457 2409
Size: 457 1952
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1652 2409
Size: 1652 757
MslI CAynn'nnrTG
Cuts at: 0 113 1291 1765 2170 2409
Size: 113 1178 474 405 239
Fragments arranged by size:
1178 474 405 239 113
MspA1I CmG'CkG
Cuts at: 0 2136 2409
Size: 2136 273
NaeI GCC'GGC
Cuts at: 0 2209 2409
Size: 2209 200
NcoI C'CATG_G
Cuts at: 0 108 2165 2281 2409
Size: 108 2057 116 128
Fragments arranged by size:
2057 128 116 108
NgoMIV G'CCGG_C
Cuts at: 0 2207 2409
Size: 2207 202
NsiI A_TGCA'T
Cuts at: 0 548 2000 2409
Size: 548 1452 409
Fragments arranged by size:
1452 548 409
NspI r_CATG'y
Cuts at: 0 550 792 1998 2409
Size: 550 242 1206 411
Fragments arranged by size:
1206 550 411 242
PflMI CCAn_nnn'nTGG
Cuts at: 0 2015 2139 2409
Size: 2015 124 270
Fragments arranged by size:
2015 270 124
PfoI T'CCnGG_A
Cuts at: 0 209 503 2409
Size: 209 294 1906
Fragments arranged by size:
1906 294 209
PmlI CAC'GTG
Cuts at: 0 735 2409
Size: 735 1674
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 627 659 1142 1174 2409
Size: 627 32 483 32 1235
Fragments arranged by size:
1235 627 483 32 32
PshAI GACnn'nnGTC
Cuts at: 0 2083 2409
Size: 2083 326
PspOMI G'GGCC_C
Cuts at: 0 20 2343 2409
Size: 20 2323 66
Fragments arranged by size:
2323 66 20
PspXI vC'TCGA_Gb
Cuts at: 0 863 2409
Size: 863 1546
PstI C_TGCA'G
Cuts at: 0 1283 2409
Size: 1283 1126
PvuII CAG'CTG
Cuts at: 0 2136 2409
Size: 2136 273
SbfI CC_TGCA'GG
Cuts at: 0 1283 2409
Size: 1283 1126
SfcI C'TryA_G
Cuts at: 0 1262 1279 1913 2409
Size: 1262 17 634 496
Fragments arranged by size:
1262 634 496 17
SmlI C'TyrA_G
Cuts at: 0 460 562 863 2409
Size: 460 102 301 1546
Fragments arranged by size:
1546 460 301 102
SphI G_CATG'C
Cuts at: 0 1998 2409
Size: 1998 411
StuI AGG'CCT
Cuts at: 0 1277 2192 2409
Size: 1277 915 217
Fragments arranged by size:
1277 915 217
StyI C'CwwG_G
Cuts at: 0 108 2165 2281 2409
Size: 108 2057 116 128
Fragments arranged by size:
2057 128 116 108
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1122 1322 1829 2409
Size: 1122 200 507 580
Fragments arranged by size:
1122 580 507 200
TatI w'GTAC_w
Cuts at: 0 836 1935 2409
Size: 836 1099 474
Fragments arranged by size:
1099 836 474
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 719 751 1712 1744 1775 1807 2409
Size: 719 32 961 32 31 32 602
Fragments arranged by size:
961 719 602 32 32 32 31
Tth111I GACn'n_nGTC
Cuts at: 0 412 2409
Size: 412 1997
XhoI C'TCGA_G
Cuts at: 0 863 2409
Size: 863 1546
XmnI GAAnn'nnTTC
Cuts at: 0 205 499 656 2409
Size: 205 294 157 1753
Fragments arranged by size:
1753 294 205 157
Enzymes that do cut:
AarI AcuI AfeI AflIII AgeI AleI AlfI AloI
AlwNI ApaI ApoI AvaI BamHI BanI BanII BbsI
BbvCI BclI BfrBI BglII Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI Bsp1286I BspHI BspMI BsrDI BsrFI BstAPI
BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DrdI
EaeI Eco57MI EcoO109I EcoRI FalI HaeII Hin4I HindIII
MmeI MslI MspA1I NaeI NcoI NgoMIV NsiI NspI
PflMI PfoI PmlI PpiI PshAI PspOMI PspXI PstI
PvuII SbfI SfcI SmlI SphI StuI StyI TaqII
TatI TstI Tth111I XhoI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AhdI ApaLI AscI
AseI AsiSI AvrII BaeI BbeI BcgI BciVI BglI
BlpI BmgBI BmtI BsaBI BsaHI BsiEI BsiWI BsmI
BsmBI BspEI BsrBI BsrGI BssHII BssSI BstBI BstEII
BstZ17I ClaI DraI DraIII EagI EarI EciI EcoICRI
EcoNI EcoRV FseI FspI FspAI HincII HpaI KasI
KpnI MfeI MluI MscI NarI NdeI NheI NotI
NruI PacI PasI PciI PmeI PpuMI PsiI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SrfI SspI SwaI XbaI XcmI XmaI ZraI