Restriction map for
pFN21AE3307
(Linear) (Six-base) MAPSORT of: KIEE3307.seq Check: 1616 from: 1 to: 2394
KIEE3307.orf
With 182 enzymes: SgfI *
July 3, 2012 17:37 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1146 2334 2394
Size: 1146 1188 60
Fragments arranged by size:
1188 1146 60
AfeI AGC'GCT
Cuts at: 0 298 2394
Size: 298 2096
AgeI A'CCGG_T
Cuts at: 0 2134 2394
Size: 2134 260
AlwNI CAG_nnn'CTG
Cuts at: 0 1113 2394
Size: 1113 1281
ApaLI G'TGCA_C
Cuts at: 0 1684 2394
Size: 1684 710
ApoI r'AATT_y
Cuts at: 0 1810 2394
Size: 1810 584
AvaI C'yCGr_G
Cuts at: 0 15 529 603 2394
Size: 15 514 74 1791
Fragments arranged by size:
1791 514 74 15
BamHI G'GATC_C
Cuts at: 0 1240 1567 2394
Size: 1240 327 827
Fragments arranged by size:
1240 827 327
BanI G'GyrC_C
Cuts at: 0 568 2394
Size: 568 1826
BanII G_rGCy'C
Cuts at: 0 315 360 2394
Size: 315 45 2034
Fragments arranged by size:
2034 315 45
BbsI GAAGACnn'nnnn_
Cuts at: 0 1850 2394
Size: 1850 544
BbvCI CC'TCA_GC
Cuts at: 0 436 448 594 2394
Size: 436 12 146 1800
Fragments arranged by size:
1800 436 146 12
BclI T'GATC_A
Cuts at: 0 1509 2394
Size: 1509 885
BlpI GC'TnA_GC
Cuts at: 0 1330 2394
Size: 1330 1064
Bme1580I G_kGCm'C
Cuts at: 0 263 1688 2394
Size: 263 1425 706
Fragments arranged by size:
1425 706 263
BmrI ACTGGGnnnn_n'
Cuts at: 0 1962 2394
Size: 1962 432
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 664 2394
Size: 664 1730
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1012 2394
Size: 1012 1382
Bpu10I CC'TnA_GC
Cuts at: 0 436 448 594 2394
Size: 436 12 146 1800
Fragments arranged by size:
1800 436 146 12
BsaBI GATnn'nnATC
Cuts at: 0 1609 2394
Size: 1609 785
BsaHI Gr'CG_yC
Cuts at: 0 152 342 2394
Size: 152 190 2052
Fragments arranged by size:
2052 190 152
BsaWI w'CCGG_w
Cuts at: 0 116 926 1636 2134 2394
Size: 116 810 710 498 260
Fragments arranged by size:
810 710 498 260 116
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 692 888 1039 1302 1305 1708 2394
Size: 692 196 151 263 3 403 686
Fragments arranged by size:
692 686 403 263 196 151 3
BseYI C'CCAG_C
Cuts at: 0 274 415 483 1083 2029 2195 2394
Size: 274 141 68 600 946 166 199
Fragments arranged by size:
946 600 274 199 166 141 68
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 396 2394
Size: 396 1998
BsiEI CG_ry'CG
Cuts at: 0 79 255 432 566 2394
Size: 79 176 177 134 1828
Fragments arranged by size:
1828 177 176 134 79
BsiHKAI G_wGCw'C
Cuts at: 0 315 360 1296 1688 1940 1967 2228 2394
Size: 315 45 936 392 252 27 261 166
Fragments arranged by size:
936 392 315 261 252 166 45 27
BsmI GAATG_Cn'
Cuts at: 0 1134 1402 1880 2394
Size: 1134 268 478 514
Fragments arranged by size:
1134 514 478 268
Bsp1286I G_dGCh'C
Cuts at: 0 263 315 360 1296 1688 1940 1967 2228
Size: 263 52 45 936 392 252 27 261
Cuts at: 2228 2394
Size: 166
Fragments arranged by size:
936 392 263 261 252 166 52 45
27
BspEI T'CCGG_A
Cuts at: 0 926 2394
Size: 926 1468
BspHI T'CATG_A
Cuts at: 0 1274 2171 2394
Size: 1274 897 223
Fragments arranged by size:
1274 897 223
BsrBI CCG'CTC
Cuts at: 0 396 608 2394
Size: 396 212 1786
Fragments arranged by size:
1786 396 212
BsrDI GCAATG_nn'
Cuts at: 0 1428 1535 2394
Size: 1428 107 859
Fragments arranged by size:
1428 859 107
BsrFI r'CCGG_y
Cuts at: 0 29 269 1265 1766 2134 2394
Size: 29 240 996 501 368 260
Fragments arranged by size:
996 501 368 260 240 29
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1375 2394
Size: 1375 1019
BstXI CCAn_nnnn'nTGG
Cuts at: 0 299 2394
Size: 299 2095
BstYI r'GATC_y
Cuts at: 0 1240 1567 1699 2394
Size: 1240 327 132 695
Fragments arranged by size:
1240 695 327 132
Bsu36I CC'TnA_GG
Cuts at: 0 721 761 2394
Size: 721 40 1633
Fragments arranged by size:
1633 721 40
BtgI C'CryG_G
Cuts at: 0 2114 2394
Size: 2114 280
BtsI GCAGTG_nn'
Cuts at: 0 103 1220 1916 1924 2394
Size: 103 1117 696 8 470
Fragments arranged by size:
1117 696 470 103 8
DraI TTT'AAA
Cuts at: 0 1068 1782 2394
Size: 1068 714 612
Fragments arranged by size:
1068 714 612
DraIII CAC_nnn'GTG
Cuts at: 0 388 2394
Size: 388 2006
DrdI GACnn_nn'nnGTC
Cuts at: 0 845 1194 2394
Size: 845 349 1200
Fragments arranged by size:
1200 845 349
EaeI y'GGCC_r
Cuts at: 0 76 252 429 459 2394
Size: 76 176 177 30 1935
Fragments arranged by size:
1935 177 176 76 30
EagI C'GGCC_G
Cuts at: 0 76 252 429 2394
Size: 76 176 177 1965
Fragments arranged by size:
1965 177 176 76
EarI CTCTTCn'nnn_
Cuts at: 0 1009 1801 2008 2394
Size: 1009 792 207 386
Fragments arranged by size:
1009 792 386 207
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 252 395 564 2394
Size: 252 143 169 1830
Fragments arranged by size:
1830 252 169 143
EcoICRI GAG'CTC
Cuts at: 0 313 358 2394
Size: 313 45 2036
Fragments arranged by size:
2036 313 45
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1012 1146 2334 2394
Size: 1012 134 1188 60
Fragments arranged by size:
1188 1012 134 60
EcoO109I rG'GnC_Cy
Cuts at: 0 19 533 1110 1411 2124 2394
Size: 19 514 577 301 713 270
Fragments arranged by size:
713 577 514 301 270 19
HaeII r_GCGC'y
Cuts at: 0 300 2394
Size: 300 2094
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 628 660 921 953 1008 1040 2394
Size: 628 32 261 32 55 32 1354
Fragments arranged by size:
1354 628 261 55 32 32 32
MfeI C'AATT_G
Cuts at: 0 1502 2394
Size: 1502 892
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 203 423 2394
Size: 203 220 1971
Fragments arranged by size:
1971 220 203
MslI CAynn'nnrTG
Cuts at: 0 694 2394
Size: 694 1700
MspA1I CmG'CkG
Cuts at: 0 72 140 2033 2195 2394
Size: 72 68 1893 162 199
Fragments arranged by size:
1893 199 162 72 68
NaeI GCC'GGC
Cuts at: 0 31 2394
Size: 31 2363
NcoI C'CATG_G
Cuts at: 0 2114 2394
Size: 2114 280
NdeI CA'TA_TG
Cuts at: 0 45 2394
Size: 45 2349
NgoMIV G'CCGG_C
Cuts at: 0 29 2394
Size: 29 2365
PasI CC'CwG_GG
Cuts at: 0 467 2394
Size: 467 1927
PflMI CCAn_nnn'nTGG
Cuts at: 0 1083 2394
Size: 1083 1311
PfoI T'CCnGG_A
Cuts at: 0 2321 2394
Size: 2321 73
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2338 2370 2394
Size: 2338 32 24
Fragments arranged by size:
2338 32 24
PpuMI rG'GwC_Cy
Cuts at: 0 19 533 1110 1411 2124 2394
Size: 19 514 577 301 713 270
Fragments arranged by size:
713 577 514 301 270 19
PshAI GACnn'nnGTC
Cuts at: 0 896 2394
Size: 896 1498
PstI C_TGCA'G
Cuts at: 0 1210 2394
Size: 1210 1184
PvuI CG_AT'CG
Cuts at: 0 566 2394
Size: 566 1828
PvuII CAG'CTG
Cuts at: 0 2033 2394
Size: 2033 361
SacI G_AGCT'C
Cuts at: 0 315 360 2394
Size: 315 45 2034
Fragments arranged by size:
2034 315 45
SanDI GG'GwC_CC
Cuts at: 0 533 2394
Size: 533 1861
SapI GCTCTTCn'nnn_
Cuts at: 0 1009 1801 2008 2394
Size: 1009 792 207 386
Fragments arranged by size:
1009 792 386 207
SfcI C'TryA_G
Cuts at: 0 1206 2163 2394
Size: 1206 957 231
Fragments arranged by size:
1206 957 231
SmaI CCC'GGG
Cuts at: 0 17 531 2394
Size: 17 514 1863
Fragments arranged by size:
1863 514 17
StyI C'CwwG_G
Cuts at: 0 263 544 683 956 973 1862 2114 2394
Size: 263 281 139 273 17 889 252 280
Fragments arranged by size:
889 281 280 273 263 252 139 17
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 653 1120 2394
Size: 653 467 1274
Fragments arranged by size:
1274 653 467
Tth111I GACn'n_nGTC
Cuts at: 0 553 2394
Size: 553 1841
XmaI C'CCGG_G
Cuts at: 0 15 529 2394
Size: 15 514 1865
Fragments arranged by size:
1865 514 15
XmnI GAAnn'nnTTC
Cuts at: 0 1092 1493 2394
Size: 1092 401 901
Fragments arranged by size:
1092 901 401
Enzymes that do cut:
AcuI AfeI AgeI AlwNI ApaLI ApoI AvaI BamHI
BanI BanII BbsI BbvCI BclI BlpI Bme1580I BmrI
BplI BpmI Bpu10I BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BsrBI
BsrDI BsrFI BstAPI BstXI BstYI Bsu36I BtgI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I HaeII Hin4I MfeI MmeI MslI MspA1I
NaeI NcoI NdeI NgoMIV PasI PflMI PfoI PpiI
PpuMI PshAI PstI PvuI PvuII SacI SanDI SapI
SfcI SmaI StyI TaqII Tth111I XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AhdI
AleI AlfI AloI ApaI AscI AseI AsiSI AvrII
BaeI BbeI BcgI BciVI BfrBI BglI BglII BmgBI
BmtI BpuEI BsaI BsaAI BsaXI BsiWI BsmBI BspMI
BsrGI BssHII BssSI BstBI BstEII BstZ17I BtgZI ClaI
CspCI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KasI KpnI MluI MscI NarI
NheI NotI NruI NsiI NspI PacI PciI PmeI
PmlI PsiI PspOMI PspXI PsrI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI TatI
TstI XbaI XcmI XhoI ZraI