Restriction map for
pFN21AE3303
(Linear) (Six-base) MAPSORT of: KIEE3303.seq Check: 7397 from: 1 to: 2256
KIEE3303.orf
With 182 enzymes: SgfI *
March 22, 2012 16:29 ..
AccI GT'mk_AC
Cuts at: 0 476 2256
Size: 476 1780
Acc65I G'GTAC_C
Cuts at: 0 1174 2256
Size: 1174 1082
AclI AA'CG_TT
Cuts at: 0 1024 2256
Size: 1024 1232
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 219 425 502 2071 2256
Size: 219 206 77 1569 185
Fragments arranged by size:
1569 219 206 185 77
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1782 2256
Size: 1782 474
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 425 457 2229 2256
Size: 425 32 1772 27
Fragments arranged by size:
1772 425 32 27
AlwNI CAG_nnn'CTG
Cuts at: 0 210 639 1426 2256
Size: 210 429 787 830
Fragments arranged by size:
830 787 429 210
ApoI r'AATT_y
Cuts at: 0 37 100 1232 1243 1696 1735 1963 2256
Size: 37 63 1132 11 453 39 228 293
Fragments arranged by size:
1132 453 293 228 63 39 37 11
AvaI C'yCGr_G
Cuts at: 0 1088 1170 2256
Size: 1088 82 1086
Fragments arranged by size:
1088 1086 82
BamHI G'GATC_C
Cuts at: 0 648 2256
Size: 648 1608
BanI G'GyrC_C
Cuts at: 0 1174 1741 1879 2256
Size: 1174 567 138 377
Fragments arranged by size:
1174 567 377 138
BanII G_rGCy'C
Cuts at: 0 427 1534 2256
Size: 427 1107 722
Fragments arranged by size:
1107 722 427
BbsI GAAGACnn'nnnn_
Cuts at: 0 153 413 443 2256
Size: 153 260 30 1813
Fragments arranged by size:
1813 260 153 30
BbvCI CC'TCA_GC
Cuts at: 0 661 2256
Size: 661 1595
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 340 374 2256
Size: 340 34 1882
Fragments arranged by size:
1882 340 34
BglII A'GATC_T
Cuts at: 0 2036 2256
Size: 2036 220
Bme1580I G_kGCm'C
Cuts at: 0 363 2256
Size: 363 1893
BmrI ACTGGGnnnn_n'
Cuts at: 0 463 627 2070 2256
Size: 463 164 1443 186
Fragments arranged by size:
1443 463 186 164
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 14 34 208 532 559 661 1286 1313
Size: 14 20 174 324 27 102 625 27
Cuts at: 1313 2256
Size: 943
Fragments arranged by size:
943 625 324 174 102 27 27 20
14
Bpu10I CC'TnA_GC
Cuts at: 0 661 1822 2256
Size: 661 1161 434
Fragments arranged by size:
1161 661 434
BsaI GGTCTCn'nnnn_
Cuts at: 0 24 250 583 1043 2256
Size: 24 226 333 460 1213
Fragments arranged by size:
1213 460 333 226 24
BsaHI Gr'CG_yC
Cuts at: 0 881 2256
Size: 881 1375
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 532 562 778 808 1615 1645 2256
Size: 532 30 216 30 807 30 611
Fragments arranged by size:
807 611 532 216 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1493 2256
Size: 1493 763
BseYI C'CCAG_C
Cuts at: 0 203 1472 1864 2256
Size: 203 1269 392 392
Fragments arranged by size:
1269 392 392 203
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 888 2078 2256
Size: 888 1190 178
Fragments arranged by size:
1190 888 178
BsiEI CG_ry'CG
Cuts at: 0 705 2256
Size: 705 1551
BsiHKAI G_wGCw'C
Cuts at: 0 217 2256
Size: 217 2039
BsmI GAATG_Cn'
Cuts at: 0 330 2256
Size: 330 1926
BsmBI CGTCTCn'nnnn_
Cuts at: 0 508 2256
Size: 508 1748
Bsp1286I G_dGCh'C
Cuts at: 0 217 363 427 1534 2256
Size: 217 146 64 1107 722
Fragments arranged by size:
1107 722 217 146 64
BspHI T'CATG_A
Cuts at: 0 173 1616 2256
Size: 173 1443 640
Fragments arranged by size:
1443 640 173
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1774 2256
Size: 1774 482
BsrDI GCAATG_nn'
Cuts at: 0 760 1652 1844 2256
Size: 760 892 192 412
Fragments arranged by size:
892 760 412 192
BsrFI r'CCGG_y
Cuts at: 0 2044 2256
Size: 2044 212
BssSI C'ACGA_G
Cuts at: 0 253 2256
Size: 253 2003
BstAPI GCAn_nnn'nTGC
Cuts at: 0 334 860 2256
Size: 334 526 1396
Fragments arranged by size:
1396 526 334
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1641 2028 2196 2256
Size: 1641 387 168 60
Fragments arranged by size:
1641 387 168 60
BstYI r'GATC_y
Cuts at: 0 648 2036 2256
Size: 648 1388 220
Fragments arranged by size:
1388 648 220
BstZ17I GTA'TAC
Cuts at: 0 477 2256
Size: 477 1779
Bsu36I CC'TnA_GG
Cuts at: 0 527 2256
Size: 527 1729
BtgI C'CryG_G
Cuts at: 0 738 2021 2256
Size: 738 1283 235
Fragments arranged by size:
1283 738 235
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 972 1007 2105 2140 2256
Size: 972 35 1098 35 116
Fragments arranged by size:
1098 972 116 35 35
EarI CTCTTCn'nnn_
Cuts at: 0 229 2047 2206 2233 2256
Size: 229 1818 159 27 23
Fragments arranged by size:
1818 229 159 27 23
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1116 2256
Size: 1116 1140
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 14 34 208 219 425 502 532 559
Size: 14 20 174 11 206 77 30 27
Cuts at: 559 661 1286 1313 2071 2256
Size: 102 625 27 758 185
Fragments arranged by size:
758 625 206 185 174 102 77 30
27 27 20 14 11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1301 2256
Size: 1301 955
EcoO109I rG'GnC_Cy
Cuts at: 0 1294 1602 2200 2256
Size: 1294 308 598 56
Fragments arranged by size:
1294 598 308 56
EcoRI G'AATT_C
Cuts at: 0 1232 2256
Size: 1232 1024
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1419 2235 2256
Size: 1419 816 21
Fragments arranged by size:
1419 816 21
HaeII r_GCGC'y
Cuts at: 0 2188 2256
Size: 2188 68
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 358 390 778 810 1040 1072 2187 2219
Size: 358 32 388 32 230 32 1115 32
Cuts at: 2219 2256
Size: 37
Fragments arranged by size:
1115 388 358 230 37 32 32 32
32
HincII GTy'rAC
Cuts at: 0 612 2256
Size: 612 1644
KpnI G_GTAC'C
Cuts at: 0 1178 2256
Size: 1178 1078
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1775 2256
Size: 1775 481
MslI CAynn'nnrTG
Cuts at: 0 874 1639 2026 2194 2256
Size: 874 765 387 168 62
Fragments arranged by size:
874 765 387 168 62
MspA1I CmG'CkG
Cuts at: 0 403 1472 1553 2256
Size: 403 1069 81 703
Fragments arranged by size:
1069 703 403 81
NcoI C'CATG_G
Cuts at: 0 738 2021 2256
Size: 738 1283 235
Fragments arranged by size:
1283 738 235
NdeI CA'TA_TG
Cuts at: 0 1075 2256
Size: 1075 1181
NspI r_CATG'y
Cuts at: 0 1872 2256
Size: 1872 384
PflMI CCAn_nnn'nTGG
Cuts at: 0 639 1889 2256
Size: 639 1250 367
Fragments arranged by size:
1250 639 367
PfoI T'CCnGG_A
Cuts at: 0 581 1835 1922 2256
Size: 581 1254 87 334
Fragments arranged by size:
1254 581 334 87
PpuMI rG'GwC_Cy
Cuts at: 0 1602 2256
Size: 1602 654
PvuII CAG'CTG
Cuts at: 0 403 2256
Size: 403 1853
SapI GCTCTTCn'nnn_
Cuts at: 0 2206 2233 2256
Size: 2206 27 23
Fragments arranged by size:
2206 27 23
SexAI A'CCwGG_T
Cuts at: 0 1598 2256
Size: 1598 658
SfcI C'TryA_G
Cuts at: 0 724 2256
Size: 724 1532
SmaI CCC'GGG
Cuts at: 0 1090 1172 2256
Size: 1090 82 1084
Fragments arranged by size:
1090 1084 82
SspI AAT'ATT
Cuts at: 0 58 67 2256
Size: 58 9 2189
Fragments arranged by size:
2189 58 9
StuI AGG'CCT
Cuts at: 0 355 525 666 896 2256
Size: 355 170 141 230 1360
Fragments arranged by size:
1360 355 230 170 141
StyI C'CwwG_G
Cuts at: 0 738 797 1130 1535 2021 2256
Size: 738 59 333 405 486 235
Fragments arranged by size:
738 486 405 333 235 59
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 437 2256
Size: 437 1819
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 478 510 1649 1681 2256
Size: 478 32 1139 32 575
Fragments arranged by size:
1139 575 478 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1600 2256
Size: 1600 656
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 762 1581 2256
Size: 762 819 675
Fragments arranged by size:
819 762 675
XmaI C'CCGG_G
Cuts at: 0 1088 1170 2256
Size: 1088 82 1086
Fragments arranged by size:
1088 1086 82
XmnI GAAnn'nnTTC
Cuts at: 0 832 1232 1315 2256
Size: 832 400 83 941
Fragments arranged by size:
941 832 400 83
Enzymes that do cut:
AccI Acc65I AclI AcuI AlfI AloI AlwNI ApoI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BglII
Bme1580I BmrI BpmI Bpu10I BsaI BsaHI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI
BspMI BsrDI BsrFI BssSI BstAPI BstXI BstYI BstZ17I
Bsu36I BtgI CspCI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI HaeII Hin4I HincII KpnI MmeI MslI
MspA1I NcoI NdeI NspI PflMI PfoI PpuMI PvuII
SapI SexAI SfcI SmaI SspI StuI StyI TaqII
TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AflIII AgeI AhdI AleI
ApaI ApaLI AscI AseI AsiSI AvrII BaeI BbeI
BciVI BclI BfrBI BglI BlpI BmgBI BmtI BplI
BpuEI BsaAI BsaBI BsaWI BsiWI BspEI BsrBI BsrGI
BssHII BstBI BstEII BtgZI BtsI ClaI DraI DraIII
DrdI EaeI EagI EcoICRI EcoRV FseI FspI FspAI
HindIII HpaI KasI MfeI MluI MscI NaeI NarI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI RsrII SacI SacII SalI SanDI SbfI
ScaI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI
SphI SrfI SwaI TatI XbaI XhoI ZraI