Restriction map for
pFN21AE3294
(Linear) (Six-base) MAPSORT of: KIEE3294.seq Check: 2266 from: 1 to: 2298
KIEE3294.orf
With 182 enzymes: SgfI *
July 3, 2012 17:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 490 1053 2298
Size: 490 563 1245
Fragments arranged by size:
1245 563 490
AccI GT'mk_AC
Cuts at: 0 1529 2135 2298
Size: 1529 606 163
Fragments arranged by size:
1529 606 163
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 195 363 504 985 1201 1377 1603 1753
Size: 195 168 141 481 216 176 226 150
Cuts at: 1753 1833 2298
Size: 80 465
Fragments arranged by size:
481 465 226 216 195 176 168 150
141 80
AflIII A'CryG_T
Cuts at: 0 1615 2288 2298
Size: 1615 673 10
Fragments arranged by size:
1615 673 10
AleI CACnn'nnGTG
Cuts at: 0 260 391 2298
Size: 260 131 1907
Fragments arranged by size:
1907 260 131
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1183 2298
Size: 1183 1115
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 829 861 2042 2074 2298
Size: 829 32 1181 32 224
Fragments arranged by size:
1181 829 224 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 288 312 369 492 831 1068 1170 1758
Size: 288 24 57 123 339 237 102 588
Cuts at: 1758 2298
Size: 540
Fragments arranged by size:
588 540 339 288 237 123 102 57
24
ApaI G_GGCC'C
Cuts at: 0 1179 2115 2298
Size: 1179 936 183
Fragments arranged by size:
1179 936 183
ApaLI G'TGCA_C
Cuts at: 0 1498 2298
Size: 1498 800
ApoI r'AATT_y
Cuts at: 0 1266 1414 1738 2298
Size: 1266 148 324 560
Fragments arranged by size:
1266 560 324 148
AvaI C'yCGr_G
Cuts at: 0 16 761 800 985 1402 1936 2298
Size: 16 745 39 185 417 534 362
Fragments arranged by size:
745 534 417 362 185 39 16
BamHI G'GATC_C
Cuts at: 0 251 2298
Size: 251 2047
BanI G'GyrC_C
Cuts at: 0 67 2298
Size: 67 2231
BanII G_rGCy'C
Cuts at: 0 963 992 1179 1442 1961 2115 2298
Size: 963 29 187 263 519 154 183
Fragments arranged by size:
963 519 263 187 183 154 29
BbsI GAAGACnn'nnnn_
Cuts at: 0 1839 2298
Size: 1839 459
BbvCI CC'TCA_GC
Cuts at: 0 1810 2298
Size: 1810 488
BclI T'GATC_A
Cuts at: 0 2162 2298
Size: 2162 136
BglI GCCn_nnn'nGGC
Cuts at: 0 410 1081 2298
Size: 410 671 1217
Fragments arranged by size:
1217 671 410
BglII A'GATC_T
Cuts at: 0 134 2298
Size: 134 2164
BlpI GC'TnA_GC
Cuts at: 0 811 1447 2298
Size: 811 636 851
Fragments arranged by size:
851 811 636
Bme1580I G_kGCm'C
Cuts at: 0 1179 1502 1650 2115 2298
Size: 1179 323 148 465 183
Fragments arranged by size:
1179 465 323 183 148
BmgBI CAC'GTC
Cuts at: 0 2291 2298
Size: 2291 7
BmrI ACTGGGnnnn_n'
Cuts at: 0 648 1827 2109 2298
Size: 648 1179 282 189
Fragments arranged by size:
1179 648 282 189
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 787 1128 1296 2298
Size: 787 341 168 1002
Fragments arranged by size:
1002 787 341 168
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 310 759 1662 2298
Size: 310 449 903 636
Fragments arranged by size:
903 636 449 310
Bpu10I CC'TnA_GC
Cuts at: 0 1810 2298
Size: 1810 488
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 420 1186 2298
Size: 420 766 1112
Fragments arranged by size:
1112 766 420
BsaI GGTCTCn'nnnn_
Cuts at: 0 88 758 2218 2298
Size: 88 670 1460 80
Fragments arranged by size:
1460 670 88 80
BsaHI Gr'CG_yC
Cuts at: 0 4 1907 1949 2298
Size: 4 1903 42 349
Fragments arranged by size:
1903 349 42 4
BsaWI w'CCGG_w
Cuts at: 0 111 2298
Size: 111 2187
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 757 787 2042 2072 2298
Size: 757 30 1255 30 226
Fragments arranged by size:
1255 757 226 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 387 448 451 1420 1984 2290 2298
Size: 387 61 3 969 564 306 8
Fragments arranged by size:
969 564 387 306 61 8 3
BseYI C'CCAG_C
Cuts at: 0 127 831 1961 2006 2298
Size: 127 704 1130 45 292
Fragments arranged by size:
1130 704 292 127 45
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 718 748 844 1381 2298
Size: 295 423 30 96 537 917
Fragments arranged by size:
917 537 423 295 96 30
BsiEI CG_ry'CG
Cuts at: 0 589 2213 2298
Size: 589 1624 85
Fragments arranged by size:
1624 589 85
BsiHKAI G_wGCw'C
Cuts at: 0 1097 1442 1502 2258 2298
Size: 1097 345 60 756 40
Fragments arranged by size:
1097 756 345 60 40
BsmI GAATG_Cn'
Cuts at: 0 623 1077 2298
Size: 623 454 1221
Fragments arranged by size:
1221 623 454
Bsp1286I G_dGCh'C
Cuts at: 0 963 992 1097 1179 1442 1502 1650 1961
Size: 963 29 105 82 263 60 148 311
Cuts at: 1961 2115 2258 2298
Size: 154 143 40
Fragments arranged by size:
963 311 263 154 148 143 105 82
60 40 29
BspEI T'CCGG_A
Cuts at: 0 111 2298
Size: 111 2187
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 89 490 1053 1299 2298
Size: 89 401 563 246 999
Fragments arranged by size:
999 563 401 246 89
BsrBI CCG'CTC
Cuts at: 0 1249 1280 2298
Size: 1249 31 1018
Fragments arranged by size:
1249 1018 31
BsrDI GCAATG_nn'
Cuts at: 0 1515 2298
Size: 1515 783
BsrFI r'CCGG_y
Cuts at: 0 143 1097 1304 2298
Size: 143 954 207 994
Fragments arranged by size:
994 954 207 143
BsrGI T'GTAC_A
Cuts at: 0 415 2298
Size: 415 1883
BssSI C'ACGA_G
Cuts at: 0 199 2125 2298
Size: 199 1926 173
Fragments arranged by size:
1926 199 173
BstAPI GCAn_nnn'nTGC
Cuts at: 0 741 1758 2298
Size: 741 1017 540
Fragments arranged by size:
1017 741 540
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2235 2298
Size: 2235 63
BstYI r'GATC_y
Cuts at: 0 134 251 2298
Size: 134 117 2047
Fragments arranged by size:
2047 134 117
BtgI C'CryG_G
Cuts at: 0 1550 2298
Size: 1550 748
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1125 2298
Size: 1125 1173
BtsI GCAGTG_nn'
Cuts at: 0 2256 2298
Size: 2256 42
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1023 1058 2298
Size: 1023 35 1240
Fragments arranged by size:
1240 1023 35
DraI TTT'AAA
Cuts at: 0 2271 2298
Size: 2271 27
DraIII CAC_nnn'GTG
Cuts at: 0 656 2298
Size: 656 1642
DrdI GACnn_nn'nnGTC
Cuts at: 0 148 2298
Size: 148 2150
EaeI y'GGCC_r
Cuts at: 0 586 861 2298
Size: 586 275 1437
Fragments arranged by size:
1437 586 275
EagI C'GGCC_G
Cuts at: 0 586 2298
Size: 586 1712
EarI CTCTTCn'nnn_
Cuts at: 0 412 896 961 1052 1258 1723 2298
Size: 412 484 65 91 206 465 575
Fragments arranged by size:
575 484 465 412 206 91 65
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 339 2050 2298
Size: 339 1711 248
Fragments arranged by size:
1711 339 248
EcoICRI GAG'CTC
Cuts at: 0 1440 2298
Size: 1440 858
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 195 310 363 504 759 985 1201 1377
Size: 195 115 53 141 255 226 216 176
Cuts at: 1377 1603 1662 1753 1833 2298
Size: 226 59 91 80 465
Fragments arranged by size:
465 255 226 226 216 195 176 141
115 91 80 59 53
EcoNI CCTnn'n_nnAGG
Cuts at: 0 262 274 2071 2298
Size: 262 12 1797 227
Fragments arranged by size:
1797 262 227 12
EcoO109I rG'GnC_Cy
Cuts at: 0 267 285 309 564 1176 2112 2298
Size: 267 18 24 255 612 936 186
Fragments arranged by size:
936 612 267 255 186 24 18
EcoRI G'AATT_C
Cuts at: 0 1414 1738 2298
Size: 1414 324 560
Fragments arranged by size:
1414 560 324
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1581 2298
Size: 1581 717
FspI TGC'GCA
Cuts at: 0 55 2298
Size: 55 2243
HaeII r_GCGC'y
Cuts at: 0 1247 2298
Size: 1247 1051
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 331 363 757 789 2002 2034 2298
Size: 331 32 394 32 1213 32 264
Fragments arranged by size:
1213 394 331 264 32 32 32
HincII GTy'rAC
Cuts at: 0 474 1434 2298
Size: 474 960 864
Fragments arranged by size:
960 864 474
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 32 728 1841 2298
Size: 32 696 1113 457
Fragments arranged by size:
1113 696 457 32
MscI TGG'CCA
Cuts at: 0 863 2298
Size: 863 1435
MslI CAynn'nnrTG
Cuts at: 0 260 391 1214 1231 2298
Size: 260 131 823 17 1067
Fragments arranged by size:
1067 823 260 131 17
MspA1I CmG'CkG
Cuts at: 0 732 975 1167 1365 1488 2298
Size: 732 243 192 198 123 810
Fragments arranged by size:
810 732 243 198 192 123
NaeI GCC'GGC
Cuts at: 0 1306 2298
Size: 1306 992
NgoMIV G'CCGG_C
Cuts at: 0 1304 2298
Size: 1304 994
NotI GC'GGCC_GC
Cuts at: 0 586 2298
Size: 586 1712
NspI r_CATG'y
Cuts at: 0 1619 1989 2298
Size: 1619 370 309
Fragments arranged by size:
1619 370 309
PasI CC'CwG_GG
Cuts at: 0 72 445 2073 2298
Size: 72 373 1628 225
Fragments arranged by size:
1628 373 225 72
PciI A'CATG_T
Cuts at: 0 1615 2298
Size: 1615 683
PflMI CCAn_nnn'nTGG
Cuts at: 0 288 390 492 1629 2298
Size: 288 102 102 1137 669
Fragments arranged by size:
1137 669 288 102 102
PfoI T'CCnGG_A
Cuts at: 0 1639 1721 2298
Size: 1639 82 577
Fragments arranged by size:
1639 577 82
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2042 2074 2298
Size: 2042 32 224
Fragments arranged by size:
2042 224 32
PpuMI rG'GwC_Cy
Cuts at: 0 267 285 309 564 2298
Size: 267 18 24 255 1734
Fragments arranged by size:
1734 267 255 24 18
PspOMI G'GGCC_C
Cuts at: 0 1175 2111 2298
Size: 1175 936 187
Fragments arranged by size:
1175 936 187
PstI C_TGCA'G
Cuts at: 0 365 886 980 2263 2298
Size: 365 521 94 1283 35
Fragments arranged by size:
1283 521 365 94 35
PvuII CAG'CTG
Cuts at: 0 732 1167 2298
Size: 732 435 1131
Fragments arranged by size:
1131 732 435
SacI G_AGCT'C
Cuts at: 0 1442 2298
Size: 1442 856
SanDI GG'GwC_CC
Cuts at: 0 564 2298
Size: 564 1734
SapI GCTCTTCn'nnn_
Cuts at: 0 1052 1723 2298
Size: 1052 671 575
Fragments arranged by size:
1052 671 575
ScaI AGT'ACT
Cuts at: 0 2149 2298
Size: 2149 149
SexAI A'CCwGG_T
Cuts at: 0 269 311 1622 2298
Size: 269 42 1311 676
Fragments arranged by size:
1311 676 269 42
SfcI C'TryA_G
Cuts at: 0 361 882 976 2259 2298
Size: 361 521 94 1283 39
Fragments arranged by size:
1283 521 361 94 39
SmlI C'TyrA_G
Cuts at: 0 399 1201 2298
Size: 399 802 1097
Fragments arranged by size:
1097 802 399
StuI AGG'CCT
Cuts at: 0 1084 1815 1942 2298
Size: 1084 731 127 356
Fragments arranged by size:
1084 731 356 127
StyI C'CwwG_G
Cuts at: 0 608 2228 2298
Size: 608 1620 70
Fragments arranged by size:
1620 608 70
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 667 2298
Size: 667 1631
TatI w'GTAC_w
Cuts at: 0 37 415 2147 2298
Size: 37 378 1732 151
Fragments arranged by size:
1732 378 151 37
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1008 1040 1972 2004 2298
Size: 1008 32 932 32 294
Fragments arranged by size:
1008 932 294 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1624 2298
Size: 1624 674
XbaI T'CTAG_A
Cuts at: 0 2293 2298
Size: 2293 5
XmnI GAAnn'nnTTC
Cuts at: 0 1717 1738 2054 2298
Size: 1717 21 316 244
Fragments arranged by size:
1717 316 244 21
Enzymes that do cut:
AarI AccI AcuI AflIII AleI AlfI AloI AlwNI
ApaI ApaLI ApoI AvaI BamHI BanI BanII BbsI
BbvCI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstXI
BstYI BtgI BtgZI BtsI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII MmeI MscI
MslI MspA1I NaeI NgoMIV NotI NspI PasI PciI
PflMI PfoI PpiI PpuMI PspOMI PstI PvuII SacI
SanDI SapI ScaI SexAI SfcI SmlI StuI StyI
TaqII TatI TstI Tth111I XbaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AscI
AseI AsiSI AvrII BaeI BbeI BcgI BciVI BfrBI
BmtI BsaAI BsaBI BsiWI BsmBI BspHI BssHII BstBI
BstEII BstZ17I Bsu36I ClaI EcoRV FseI FspAI HindIII
HpaI KasI KpnI MfeI MluI NarI NcoI NdeI
NheI NruI NsiI PacI PmeI PmlI PshAI PsiI
PspXI PsrI PvuI RsrII SacII SalI SbfI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI SwaI XcmI XhoI XmaI ZraI