Restriction map for
pFN21AE3293
(Linear) (Six-base) MAPSORT of: KIEE3293.seq Check: 1338 from: 1 to: 2295
KIEE3293.orf
With 182 enzymes: SgfI *
July 3, 2012 17:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1119 1643 2295
Size: 1119 524 652
Fragments arranged by size:
1119 652 524
AatII G_ACGT'C
Cuts at: 0 2159 2295
Size: 2159 136
AccI GT'mk_AC
Cuts at: 0 1157 2295
Size: 1157 1138
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 81 1006 1030 1660 2295
Size: 81 925 24 630 635
Fragments arranged by size:
925 635 630 81 24
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1750 1913 2200 2295
Size: 1750 163 287 95
Fragments arranged by size:
1750 287 163 95
AlwNI CAG_nnn'CTG
Cuts at: 0 128 279 344 981 2295
Size: 128 151 65 637 1314
Fragments arranged by size:
1314 637 151 128 65
ApaI G_GGCC'C
Cuts at: 0 1020 1290 2295
Size: 1020 270 1005
Fragments arranged by size:
1020 1005 270
ApoI r'AATT_y
Cuts at: 0 479 1041 2295
Size: 479 562 1254
Fragments arranged by size:
1254 562 479
AvaI C'yCGr_G
Cuts at: 0 949 1050 2295
Size: 949 101 1245
Fragments arranged by size:
1245 949 101
BanI G'GyrC_C
Cuts at: 0 74 2143 2295
Size: 74 2069 152
Fragments arranged by size:
2069 152 74
BanII G_rGCy'C
Cuts at: 0 1020 1290 2295
Size: 1020 270 1005
Fragments arranged by size:
1020 1005 270
BbeI G_GCGC'C
Cuts at: 0 78 2147 2295
Size: 78 2069 148
Fragments arranged by size:
2069 148 78
BbsI GAAGACnn'nnnn_
Cuts at: 0 36 2295
Size: 36 2259
BbvCI CC'TCA_GC
Cuts at: 0 1475 2295
Size: 1475 820
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1213 1247 2295
Size: 1213 34 1048
Fragments arranged by size:
1213 1048 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1177 2295
Size: 1177 1118
BfrBI ATG'CAT
Cuts at: 0 471 2295
Size: 471 1824
BglI GCCn_nnn'nGGC
Cuts at: 0 2092 2295
Size: 2092 203
BlpI GC'TnA_GC
Cuts at: 0 562 1565 2295
Size: 562 1003 730
Fragments arranged by size:
1003 730 562
Bme1580I G_kGCm'C
Cuts at: 0 1020 1236 1290 2295
Size: 1020 216 54 1005
Fragments arranged by size:
1020 1005 216 54
BmgBI CAC'GTC
Cuts at: 0 1161 2295
Size: 1161 1134
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 563 2295
Size: 563 1732
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 610 1837 2295
Size: 337 273 1227 458
Fragments arranged by size:
1227 458 337 273
Bpu10I CC'TnA_GC
Cuts at: 0 355 1475 1738 2295
Size: 355 1120 263 557
Fragments arranged by size:
1120 557 355 263
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 421 1777 2086 2295
Size: 421 1356 309 209
Fragments arranged by size:
1356 421 309 209
BsaI GGTCTCn'nnnn_
Cuts at: 0 370 1446 2295
Size: 370 1076 849
Fragments arranged by size:
1076 849 370
BsaBI GATnn'nnATC
Cuts at: 0 703 2295
Size: 703 1592
BsaHI Gr'CG_yC
Cuts at: 0 75 176 2144 2156 2295
Size: 75 101 1968 12 139
Fragments arranged by size:
1968 139 101 75 12
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2180 2210 2295
Size: 2180 30 85
Fragments arranged by size:
2180 85 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1927 2019 2295
Size: 1927 92 276
Fragments arranged by size:
1927 276 92
BseYI C'CCAG_C
Cuts at: 0 27 770 800 1283 2147 2295
Size: 27 743 30 483 864 148
Fragments arranged by size:
864 743 483 148 30 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 385 1279 1388 1576 1675 1873 1883 2160
Size: 385 894 109 188 99 198 10 277
Cuts at: 2160 2295
Size: 135
Fragments arranged by size:
894 385 277 198 188 135 109 99
10
BsiEI CG_ry'CG
Cuts at: 0 1035 2295
Size: 1035 1260
BsiHKAI G_wGCw'C
Cuts at: 0 1863 2011 2295
Size: 1863 148 284
Fragments arranged by size:
1863 284 148
Bsp1286I G_dGCh'C
Cuts at: 0 1020 1236 1290 1863 2011 2295
Size: 1020 216 54 573 148 284
Fragments arranged by size:
1020 573 284 216 148 54
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1119 1472 1643 2295
Size: 1119 353 171 652
Fragments arranged by size:
1119 652 353 171
BsrBI CCG'CTC
Cuts at: 0 1608 1651 1676 2295
Size: 1608 43 25 619
Fragments arranged by size:
1608 619 43 25
BsrGI T'GTAC_A
Cuts at: 0 626 2295
Size: 626 1669
BssHII G'CGCG_C
Cuts at: 0 1330 2295
Size: 1330 965
BssSI C'ACGA_G
Cuts at: 0 1150 2295
Size: 1150 1145
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1252 2295
Size: 1252 1043
BstEII G'GTnAC_C
Cuts at: 0 1086 2295
Size: 1086 1209
BstXI CCAn_nnnn'nTGG
Cuts at: 0 507 1269 2295
Size: 507 762 1026
Fragments arranged by size:
1026 762 507
BtgI C'CryG_G
Cuts at: 0 1347 1905 2295
Size: 1347 558 390
Fragments arranged by size:
1347 558 390
BtsI GCAGTG_nn'
Cuts at: 0 868 1125 1291 1325 1588 1687 1694 1930
Size: 868 257 166 34 263 99 7 236
Cuts at: 1930 1945 2295
Size: 15 350
Fragments arranged by size:
868 350 263 257 236 166 99 34
15 7
DraIII CAC_nnn'GTG
Cuts at: 0 249 1252 2295
Size: 249 1003 1043
Fragments arranged by size:
1043 1003 249
EaeI y'GGCC_r
Cuts at: 0 83 302 386 757 911 1499 1512 2093
Size: 83 219 84 371 154 588 13 581
Cuts at: 2093 2252 2295
Size: 159 43
Fragments arranged by size:
588 581 371 219 159 154 84 83
43 13
EarI CTCTTCn'nnn_
Cuts at: 0 814 1397 1636 2295
Size: 814 583 239 659
Fragments arranged by size:
814 659 583 239
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 147 2295
Size: 147 2148
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 81 337 610 1006 1030 1660 1837 2295
Size: 81 256 273 396 24 630 177 458
Fragments arranged by size:
630 458 396 273 256 177 81 24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 319 1468 2295
Size: 319 1149 827
Fragments arranged by size:
1149 827 319
EcoO109I rG'GnC_Cy
Cuts at: 0 603 610 810 906 1017 1287 1352 2295
Size: 603 7 200 96 111 270 65 943
Fragments arranged by size:
943 603 270 200 111 96 65 7
HaeII r_GCGC'y
Cuts at: 0 78 129 2147 2295
Size: 78 51 2018 148
Fragments arranged by size:
2018 148 78 51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 979 1011 1812 1844 2295
Size: 979 32 801 32 451
Fragments arranged by size:
979 801 451 32 32
KasI G'GCGC_C
Cuts at: 0 74 2143 2295
Size: 74 2069 152
Fragments arranged by size:
2069 152 74
MfeI C'AATT_G
Cuts at: 0 638 2295
Size: 638 1657
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1055 1538 1653 2295
Size: 1055 483 115 642
Fragments arranged by size:
1055 642 483 115
MscI TGG'CCA
Cuts at: 0 85 304 388 759 913 1514 2254 2295
Size: 85 219 84 371 154 601 740 41
Fragments arranged by size:
740 601 371 219 154 85 84 41
MslI CAynn'nnrTG
Cuts at: 0 523 694 2048 2295
Size: 523 171 1354 247
Fragments arranged by size:
1354 523 247 171
MspA1I CmG'CkG
Cuts at: 0 89 1702 2151 2295
Size: 89 1613 449 144
Fragments arranged by size:
1613 449 144 89
NarI GG'CG_CC
Cuts at: 0 75 2144 2295
Size: 75 2069 151
Fragments arranged by size:
2069 151 75
NsiI A_TGCA'T
Cuts at: 0 473 2295
Size: 473 1822
NspI r_CATG'y
Cuts at: 0 471 475 561 726 2295
Size: 471 4 86 165 1569
Fragments arranged by size:
1569 471 165 86 4
PasI CC'CwG_GG
Cuts at: 0 279 1113 2295
Size: 279 834 1182
Fragments arranged by size:
1182 834 279
PflMI CCAn_nnn'nTGG
Cuts at: 0 777 1104 1833 2295
Size: 777 327 729 462
Fragments arranged by size:
777 729 462 327
PfoI T'CCnGG_A
Cuts at: 0 455 587 1340 2159 2295
Size: 455 132 753 819 136
Fragments arranged by size:
819 753 455 136 132
PpuMI rG'GwC_Cy
Cuts at: 0 610 810 2295
Size: 610 200 1485
Fragments arranged by size:
1485 610 200
PspOMI G'GGCC_C
Cuts at: 0 1016 1286 2295
Size: 1016 270 1009
Fragments arranged by size:
1016 1009 270
PstI C_TGCA'G
Cuts at: 0 878 1701 1940 1965 2295
Size: 878 823 239 25 330
Fragments arranged by size:
878 823 330 239 25
PvuII CAG'CTG
Cuts at: 0 1702 2151 2295
Size: 1702 449 144
Fragments arranged by size:
1702 449 144
SfcI C'TryA_G
Cuts at: 0 736 874 1697 1936 1961 2295
Size: 736 138 823 239 25 334
Fragments arranged by size:
823 736 334 239 138 25
SfoI GGC'GCC
Cuts at: 0 76 2145 2295
Size: 76 2069 150
Fragments arranged by size:
2069 150 76
SmaI CCC'GGG
Cuts at: 0 951 2295
Size: 951 1344
SmlI C'TyrA_G
Cuts at: 0 436 1756 2101 2295
Size: 436 1320 345 194
Fragments arranged by size:
1320 436 345 194
SphI G_CATG'C
Cuts at: 0 561 726 2295
Size: 561 165 1569
Fragments arranged by size:
1569 561 165
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 349 1783 1846 2251 2295
Size: 349 1434 63 405 44
Fragments arranged by size:
1434 405 349 63 44
TatI w'GTAC_w
Cuts at: 0 530 626 2295
Size: 530 96 1669
Fragments arranged by size:
1669 530 96
XbaI T'CTAG_A
Cuts at: 0 834 2295
Size: 834 1461
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 312 1177 2295
Size: 312 865 1118
Fragments arranged by size:
1118 865 312
XmaI C'CCGG_G
Cuts at: 0 949 2295
Size: 949 1346
ZraI GAC'GTC
Cuts at: 0 2157 2295
Size: 2157 138
Enzymes that do cut:
AarI AatII AccI AcuI AlfI AlwNI ApaI ApoI
AvaI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BfrBI BglI BlpI Bme1580I BmgBI BplI BpmI Bpu10I
BpuEI BsaI BsaBI BsaHI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrGI BssHII BssSI
BstAPI BstEII BstXI BtgI BtsI DraIII EaeI EarI
EciI Eco57MI EcoNI EcoO109I HaeII Hin4I KasI MfeI
MmeI MscI MslI MspA1I NarI NsiI NspI PasI
PflMI PfoI PpuMI PspOMI PstI PvuII SfcI SfoI
SmaI SmlI SphI TaqII TatI XbaI XcmI XmaI
ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI
AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BclI BglII BmrI BmtI BsaAI BsaWI BsiWI BsmI
BsmBI BspEI BspHI BsrDI BsrFI BstBI BstYI BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DrdI EagI EcoICRI
EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI KpnI MluI NaeI NcoI NdeI NgoMIV NheI
NotI NruI PacI PciI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI
SnaBI SpeI SrfI SspI StuI StyI SwaI TstI
Tth111I XhoI XmnI