Restriction map for
pFN21AE3291
(Linear) (Six-base) MAPSORT of: KIEE3291.seq Check: 702 from: 1 to: 2259
KIEE3291.orf
With 182 enzymes: SgfI *
March 22, 2012 16:29 ..
AatII G_ACGT'C
Cuts at: 0 2138 2259
Size: 2138 121
AccI GT'mk_AC
Cuts at: 0 1393 1634 2259
Size: 1393 241 625
Fragments arranged by size:
1393 625 241
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 418 1123 2259
Size: 418 705 1136
Fragments arranged by size:
1136 705 418
AflIII A'CryG_T
Cuts at: 0 933 1051 2259
Size: 933 118 1208
Fragments arranged by size:
1208 933 118
AleI CACnn'nnGTG
Cuts at: 0 316 2259
Size: 316 1943
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1175 1207 2259
Size: 1175 32 1052
Fragments arranged by size:
1175 1052 32
AlwNI CAG_nnn'CTG
Cuts at: 0 594 998 1471 2259
Size: 594 404 473 788
Fragments arranged by size:
788 594 473 404
ApoI r'AATT_y
Cuts at: 0 1249 1490 1672 2259
Size: 1249 241 182 587
Fragments arranged by size:
1249 587 241 182
AseI AT'TA_AT
Cuts at: 0 1430 2259
Size: 1430 829
AvaI C'yCGr_G
Cuts at: 0 1532 2259
Size: 1532 727
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1710 1744 2259
Size: 1710 34 515
Fragments arranged by size:
1710 515 34
BclI T'GATC_A
Cuts at: 0 1037 2259
Size: 1037 1222
BmgBI CAC'GTC
Cuts at: 0 936 1054 2172 2259
Size: 936 118 1118 87
Fragments arranged by size:
1118 936 118 87
BmrI ACTGGGnnnn_n'
Cuts at: 0 1801 2259
Size: 1801 458
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1296 1596 2259
Size: 1296 300 663
Fragments arranged by size:
1296 663 300
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1268 2259
Size: 1268 991
BsaBI GATnn'nnATC
Cuts at: 0 472 2259
Size: 472 1787
BsaHI Gr'CG_yC
Cuts at: 0 2135 2259
Size: 2135 124
BsaWI w'CCGG_w
Cuts at: 0 2095 2259
Size: 2095 164
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1756 1786 2259
Size: 1756 30 473
Fragments arranged by size:
1756 473 30
BseYI C'CCAG_C
Cuts at: 0 2211 2259
Size: 2211 48
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 170 555 602 944 1117 2185 2259
Size: 170 385 47 342 173 1068 74
Fragments arranged by size:
1068 385 342 173 170 74 47
BsmI GAATG_Cn'
Cuts at: 0 774 1551 2259
Size: 774 777 708
Fragments arranged by size:
777 774 708
BspEI T'CCGG_A
Cuts at: 0 2095 2259
Size: 2095 164
BspHI T'CATG_A
Cuts at: 0 48 410 2259
Size: 48 362 1849
Fragments arranged by size:
1849 362 48
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1922 2259
Size: 1922 337
BsrDI GCAATG_nn'
Cuts at: 0 2001 2259
Size: 2001 258
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1131 2259
Size: 1131 1128
BstYI r'GATC_y
Cuts at: 0 2252 2259
Size: 2252 7
BstZ17I GTA'TAC
Cuts at: 0 1394 2259
Size: 1394 865
Bsu36I CC'TnA_GG
Cuts at: 0 1591 2259
Size: 1591 668
BtgI C'CryG_G
Cuts at: 0 341 1764 1842 2259
Size: 341 1423 78 417
Fragments arranged by size:
1423 417 341 78
BtsI GCAGTG_nn'
Cuts at: 0 585 1129 1237 2259
Size: 585 544 108 1022
Fragments arranged by size:
1022 585 544 108
ClaI AT'CG_AT
Cuts at: 0 1223 2259
Size: 1223 1036
DraIII CAC_nnn'GTG
Cuts at: 0 756 2259
Size: 756 1503
EaeI y'GGCC_r
Cuts at: 0 449 1322 1518 1677 2105 2259
Size: 449 873 196 159 428 154
Fragments arranged by size:
873 449 428 196 159 154
EarI CTCTTCn'nnn_
Cuts at: 0 1059 1415 2259
Size: 1059 356 844
Fragments arranged by size:
1059 844 356
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1678 2259
Size: 1678 581
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 418 1123 2259
Size: 418 705 1136
Fragments arranged by size:
1136 705 418
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1535 1881 2259
Size: 1535 346 378
Fragments arranged by size:
1535 378 346
EcoO109I rG'GnC_Cy
Cuts at: 0 43 1595 1911 2259
Size: 43 1552 316 348
Fragments arranged by size:
1552 348 316 43
EcoRV GAT'ATC
Cuts at: 0 408 599 2259
Size: 408 191 1660
Fragments arranged by size:
1660 408 191
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 706 738 2259
Size: 706 32 1521
Fragments arranged by size:
1521 706 32
HincII GTy'rAC
Cuts at: 0 2199 2259
Size: 2199 60
MfeI C'AATT_G
Cuts at: 0 314 2259
Size: 314 1945
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 996 2259
Size: 996 1263
MscI TGG'CCA
Cuts at: 0 451 1679 2107 2259
Size: 451 1228 428 152
Fragments arranged by size:
1228 451 428 152
MslI CAynn'nnrTG
Cuts at: 0 316 560 697 784 1751 2259
Size: 316 244 137 87 967 508
Fragments arranged by size:
967 508 316 244 137 87
MspA1I CmG'CkG
Cuts at: 0 998 2026 2259
Size: 998 1028 233
Fragments arranged by size:
1028 998 233
NcoI C'CATG_G
Cuts at: 0 341 1842 2259
Size: 341 1501 417
Fragments arranged by size:
1501 417 341
NspI r_CATG'y
Cuts at: 0 538 702 2259
Size: 538 164 1557
Fragments arranged by size:
1557 538 164
PfoI T'CCnGG_A
Cuts at: 0 1094 2259
Size: 1094 1165
PpuMI rG'GwC_Cy
Cuts at: 0 43 1595 1911 2259
Size: 43 1552 316 348
Fragments arranged by size:
1552 348 316 43
PshAI GACnn'nnGTC
Cuts at: 0 835 2259
Size: 835 1424
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 664 696 1384 1416 2259
Size: 664 32 688 32 843
Fragments arranged by size:
843 688 664 32 32
PstI C_TGCA'G
Cuts at: 0 23 1464 1489 1788 2259
Size: 23 1441 25 299 471
Fragments arranged by size:
1441 471 299 25 23
PvuII CAG'CTG
Cuts at: 0 998 2026 2259
Size: 998 1028 233
Fragments arranged by size:
1028 998 233
SfcI C'TryA_G
Cuts at: 0 19 745 1441 1460 1485 1784 2259
Size: 19 726 696 19 25 299 475
Fragments arranged by size:
726 696 475 299 25 19 19
SmlI C'TyrA_G
Cuts at: 0 1283 2259
Size: 1283 976
SspI AAT'ATT
Cuts at: 0 852 1121 2259
Size: 852 269 1138
Fragments arranged by size:
1138 852 269
StuI AGG'CCT
Cuts at: 0 359 2152 2259
Size: 359 1793 107
Fragments arranged by size:
1793 359 107
StyI C'CwwG_G
Cuts at: 0 341 1195 1319 1680 1842 2259
Size: 341 854 124 361 162 417
Fragments arranged by size:
854 417 361 341 162 124
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1123 1835 1948 2203 2259
Size: 1123 712 113 255 56
Fragments arranged by size:
1123 712 255 113 56
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 583 615 1019 1051 1756 1788 2259
Size: 583 32 404 32 705 32 471
Fragments arranged by size:
705 583 471 404 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 546 2229 2259
Size: 546 1683 30
Fragments arranged by size:
1683 546 30
ZraI GAC'GTC
Cuts at: 0 2136 2259
Size: 2136 123
Enzymes that do cut:
AatII AccI AcuI AflIII AleI AloI AlwNI ApoI
AseI AvaI BcgI BclI BmgBI BmrI BplI BpuEI
BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsmI BspEI
BspHI BspMI BsrDI BstAPI BstYI BstZ17I Bsu36I BtgI
BtsI ClaI DraIII EaeI EarI EciI Eco57MI EcoNI
EcoO109I EcoRV Hin4I HincII MfeI MmeI MscI MslI
MspA1I NcoI NspI PfoI PpuMI PshAI PsrI PstI
PvuII SfcI SmlI SspI StuI StyI TaqII TstI
XcmI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AgeI AhdI AlfI
ApaI ApaLI AscI AsiSI AvrII BaeI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BfrBI BglI BglII
BlpI Bme1580I BmtI BpmI Bpu10I BsaI BsaAI BseRI
BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BsrBI BsrFI BsrGI
BssHII BssSI BstBI BstEII BstXI BtgZI CspCI DraI
DrdI EagI EcoICRI EcoRI FalI FseI FspI FspAI
HaeII HindIII HpaI KasI KpnI MluI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PflMI PmeI PmlI PpiI PsiI PspOMI PspXI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SwaI TatI Tth111I XbaI XhoI
XmaI XmnI