Restriction map for
pFN21AE3286
(Linear) (Six-base) MAPSORT of: KIEE3286.seq Check: 8702 from: 1 to: 2199
KIEE3286.orf
With 182 enzymes: SgfI *
December 27, 2011 16:34 ..
AccI GT'mk_AC
Cuts at: 0 492 2199
Size: 492 1707
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1403 2147 2199
Size: 1403 744 52
Fragments arranged by size:
1403 744 52
AflIII A'CryG_T
Cuts at: 0 603 2199
Size: 603 1596
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1350 2199
Size: 1350 849
AlwNI CAG_nnn'CTG
Cuts at: 0 169 2125 2140 2199
Size: 169 1956 15 59
Fragments arranged by size:
1956 169 59 15
ApoI r'AATT_y
Cuts at: 0 276 300 382 426 451 680 930 1481
Size: 276 24 82 44 25 229 250 551
Cuts at: 1481 1851 2199
Size: 370 348
Fragments arranged by size:
551 370 348 276 250 229 82 44
25 24
AseI AT'TA_AT
Cuts at: 0 1687 2199
Size: 1687 512
AvaI C'yCGr_G
Cuts at: 0 83 2199
Size: 83 2116
BanI G'GyrC_C
Cuts at: 0 611 2199
Size: 611 1588
BanII G_rGCy'C
Cuts at: 0 546 2199
Size: 546 1653
BbeI G_GCGC'C
Cuts at: 0 615 2199
Size: 615 1584
BbvCI CC'TCA_GC
Cuts at: 0 812 2199
Size: 812 1387
BclI T'GATC_A
Cuts at: 0 1844 2199
Size: 1844 355
BfrBI ATG'CAT
Cuts at: 0 1086 1735 2199
Size: 1086 649 464
Fragments arranged by size:
1086 649 464
BglII A'GATC_T
Cuts at: 0 1093 2199
Size: 1093 1106
Bme1580I G_kGCm'C
Cuts at: 0 373 2199
Size: 373 1826
BmrI ACTGGGnnnn_n'
Cuts at: 0 866 2199
Size: 866 1333
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 140 406 2111 2199
Size: 55 85 266 1705 88
Fragments arranged by size:
1705 266 88 85 55
Bpu10I CC'TnA_GC
Cuts at: 0 812 2199
Size: 812 1387
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1961 2199
Size: 1961 238
BsaBI GATnn'nnATC
Cuts at: 0 472 2199
Size: 472 1727
BsaHI Gr'CG_yC
Cuts at: 0 612 1242 2199
Size: 612 630 957
Fragments arranged by size:
957 630 612
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 31 34 58 235 2199
Size: 31 3 24 177 1964
Fragments arranged by size:
1964 177 31 24 3
BseYI C'CCAG_C
Cuts at: 0 94 102 162 2199
Size: 94 8 60 2037
Fragments arranged by size:
2037 94 60 8
BsmI GAATG_Cn'
Cuts at: 0 1153 1634 2199
Size: 1153 481 565
Fragments arranged by size:
1153 565 481
Bsp1286I G_dGCh'C
Cuts at: 0 373 546 2199
Size: 373 173 1653
Fragments arranged by size:
1653 373 173
BsrDI GCAATG_nn'
Cuts at: 0 1811 2199
Size: 1811 388
BsrGI T'GTAC_A
Cuts at: 0 705 793 2199
Size: 705 88 1406
Fragments arranged by size:
1406 705 88
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1324 2199
Size: 1324 875
BstEII G'GTnAC_C
Cuts at: 0 1056 2199
Size: 1056 1143
BstXI CCAn_nnnn'nTGG
Cuts at: 0 537 2199
Size: 537 1662
BstYI r'GATC_y
Cuts at: 0 1093 2199
Size: 1093 1106
Bsu36I CC'TnA_GG
Cuts at: 0 218 374 674 2199
Size: 218 156 300 1525
Fragments arranged by size:
1525 300 218 156
BtgI C'CryG_G
Cuts at: 0 1650 2199
Size: 1650 549
BtsI GCAGTG_nn'
Cuts at: 0 659 1319 1370 2199
Size: 659 660 51 829
Fragments arranged by size:
829 660 659 51
DraI TTT'AAA
Cuts at: 0 965 2052 2066 2199
Size: 965 1087 14 133
Fragments arranged by size:
1087 965 133 14
DrdI GACnn_nn'nnGTC
Cuts at: 0 359 2199
Size: 359 1840
EarI CTCTTCn'nnn_
Cuts at: 0 1469 1649 2199
Size: 1469 180 550
Fragments arranged by size:
1469 550 180
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 28 2199
Size: 28 2171
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 140 406 1403 2111 2147 2199
Size: 55 85 266 997 708 36 52
Fragments arranged by size:
997 708 266 85 55 52 36
EcoNI CCTnn'n_nnAGG
Cuts at: 0 37 178 2199
Size: 37 141 2021
Fragments arranged by size:
2021 141 37
EcoO109I rG'GnC_Cy
Cuts at: 0 69 99 1411 2199
Size: 69 30 1312 788
Fragments arranged by size:
1312 788 69 30
EcoRV GAT'ATC
Cuts at: 0 1203 2199
Size: 1203 996
HaeII r_GCGC'y
Cuts at: 0 615 2199
Size: 615 1584
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 622 654 1446 1478 2199
Size: 622 32 792 32 721
Fragments arranged by size:
792 721 622 32 32
HincII GTy'rAC
Cuts at: 0 493 1749 2199
Size: 493 1256 450
Fragments arranged by size:
1256 493 450
HindIII A'AGCT_T
Cuts at: 0 406 2199
Size: 406 1793
KasI G'GCGC_C
Cuts at: 0 611 2199
Size: 611 1588
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 2199
Size: 163 2036
MspA1I CmG'CkG
Cuts at: 0 1530 2199
Size: 1530 669
NarI GG'CG_CC
Cuts at: 0 612 2199
Size: 612 1587
NcoI C'CATG_G
Cuts at: 0 1650 2199
Size: 1650 549
NsiI A_TGCA'T
Cuts at: 0 1088 1737 2199
Size: 1088 649 462
Fragments arranged by size:
1088 649 462
NspI r_CATG'y
Cuts at: 0 607 1234 1557 2199
Size: 607 627 323 642
Fragments arranged by size:
642 627 607 323
PciI A'CATG_T
Cuts at: 0 603 2199
Size: 603 1596
PflMI CCAn_nnn'nTGG
Cuts at: 0 1656 2199
Size: 1656 543
PpuMI rG'GwC_Cy
Cuts at: 0 69 99 1411 2199
Size: 69 30 1312 788
Fragments arranged by size:
1312 788 69 30
PshAI GACnn'nnGTC
Cuts at: 0 1876 2199
Size: 1876 323
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 549 581 2199
Size: 549 32 1618
Fragments arranged by size:
1618 549 32
PstI C_TGCA'G
Cuts at: 0 1314 1329 2191 2199
Size: 1314 15 862 8
Fragments arranged by size:
1314 862 15 8
PvuII CAG'CTG
Cuts at: 0 1530 2199
Size: 1530 669
SalI G'TCGA_C
Cuts at: 0 491 2199
Size: 491 1708
SanDI GG'GwC_CC
Cuts at: 0 99 2199
Size: 99 2100
ScaI AGT'ACT
Cuts at: 0 1355 1884 2199
Size: 1355 529 315
Fragments arranged by size:
1355 529 315
SfcI C'TryA_G
Cuts at: 0 1196 1310 1325 1784 2187 2199
Size: 1196 114 15 459 403 12
Fragments arranged by size:
1196 459 403 114 15 12
SfoI GGC'GCC
Cuts at: 0 613 2199
Size: 613 1586
SmaI CCC'GGG
Cuts at: 0 85 2199
Size: 85 2114
SmlI C'TyrA_G
Cuts at: 0 1976 2199
Size: 1976 223
SphI G_CATG'C
Cuts at: 0 1557 2199
Size: 1557 642
SspI AAT'ATT
Cuts at: 0 788 1050 1276 2199
Size: 788 262 226 923
Fragments arranged by size:
923 788 262 226
StyI C'CwwG_G
Cuts at: 0 1173 1220 1650 2199
Size: 1173 47 430 549
Fragments arranged by size:
1173 549 430 47
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2008 2199
Size: 2008 191
TatI w'GTAC_w
Cuts at: 0 705 793 1077 1353 1882 2069 2199
Size: 705 88 284 276 529 187 130
Fragments arranged by size:
705 529 284 276 187 130 88
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1118 1150 2199
Size: 1118 32 1049
Fragments arranged by size:
1118 1049 32
XbaI T'CTAG_A
Cuts at: 0 270 837 1113 1143 2199
Size: 270 567 276 30 1056
Fragments arranged by size:
1056 567 276 270 30
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 861 2199
Size: 861 1338
XmaI C'CCGG_G
Cuts at: 0 83 2199
Size: 83 2116
XmnI GAAnn'nnTTC
Cuts at: 0 1643 2199
Size: 1643 556
Enzymes that do cut:
AccI AcuI AflIII AlfI AlwNI ApoI AseI AvaI
BanI BanII BbeI BbvCI BclI BfrBI BglII Bme1580I
BmrI BpmI Bpu10I BpuEI BsaBI BsaHI BseRI BseYI
BsmI Bsp1286I BsrDI BsrGI BstAPI BstEII BstXI BstYI
Bsu36I BtgI BtsI DraI DrdI EarI EciI Eco57MI
EcoNI EcoO109I EcoRV HaeII Hin4I HincII HindIII KasI
MmeI MspA1I NarI NcoI NsiI NspI PciI PflMI
PpuMI PshAI PsrI PstI PvuII SalI SanDI ScaI
SfcI SfoI SmaI SmlI SphI SspI StyI TaqII
TatI TstI XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AloI ApaI ApaLI AscI AsiSI AvrII BaeI
BamHI BbsI BcgI BciVI BglI BlpI BmgBI BmtI
BplI BsaI BsaAI BsaWI BsaXI BsgI BsiEI BsiHKAI
BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrFI BssHII
BssSI BstBI BstZ17I BtgZI ClaI CspCI DraIII EaeI
EagI EcoICRI EcoRI FalI FseI FspI FspAI HpaI
KpnI MfeI MluI MscI MslI NaeI NdeI NgoMIV
NheI NotI NruI PacI PasI PfoI PmeI PmlI
PpiI PsiI PspOMI PspXI PvuI RsrII SacI SacII
SapI SbfI SexAI SfiI SgfI SgrAI SnaBI SpeI
SrfI StuI SwaI Tth111I XhoI ZraI