Restriction map for
pFN21AE3282
(Linear) (Six-base) MAPSORT of: KIEE3282.seq Check: 7713 from: 1 to: 2142
KIEE3282.orf
With 182 enzymes: SgfI *
July 3, 2012 17:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 536 2142
Size: 536 1606
AccI GT'mk_AC
Cuts at: 0 833 2142
Size: 833 1309
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1465 2142
Size: 1465 677
AfeI AGC'GCT
Cuts at: 0 1159 2142
Size: 1159 983
AflIII A'CryG_T
Cuts at: 0 755 2142
Size: 755 1387
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 460 2060 2142
Size: 460 1600 82
Fragments arranged by size:
1600 460 82
AlwNI CAG_nnn'CTG
Cuts at: 0 942 1196 1950 2034 2142
Size: 942 254 754 84 108
Fragments arranged by size:
942 754 254 108 84
ApaI G_GGCC'C
Cuts at: 0 1239 1625 2142
Size: 1239 386 517
Fragments arranged by size:
1239 517 386
ApoI r'AATT_y
Cuts at: 0 570 1288 1776 2142
Size: 570 718 488 366
Fragments arranged by size:
718 570 488 366
AvaI C'yCGr_G
Cuts at: 0 1032 1584 1800 2142
Size: 1032 552 216 342
Fragments arranged by size:
1032 552 342 216
BamHI G'GATC_C
Cuts at: 0 635 651 909 2142
Size: 635 16 258 1233
Fragments arranged by size:
1233 635 258 16
BanI G'GyrC_C
Cuts at: 0 42 874 2142
Size: 42 832 1268
Fragments arranged by size:
1268 832 42
BanII G_rGCy'C
Cuts at: 0 26 214 1033 1239 1610 1625 1960 2142
Size: 26 188 819 206 371 15 335 182
Fragments arranged by size:
819 371 335 206 188 182 26 15
BbeI G_GCGC'C
Cuts at: 0 46 2142
Size: 46 2096
BbsI GAAGACnn'nnnn_
Cuts at: 0 593 1658 2142
Size: 593 1065 484
Fragments arranged by size:
1065 593 484
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1023 1057 2142
Size: 1023 34 1085
Fragments arranged by size:
1085 1023 34
BclI T'GATC_A
Cuts at: 0 1085 2142
Size: 1085 1057
BglI GCCn_nnn'nGGC
Cuts at: 0 434 2142
Size: 434 1708
BglII A'GATC_T
Cuts at: 0 452 1023 1281 2142
Size: 452 571 258 861
Fragments arranged by size:
861 571 452 258
Bme1580I G_kGCm'C
Cuts at: 0 134 430 986 1239 1625 2142
Size: 134 296 556 253 386 517
Fragments arranged by size:
556 517 386 296 253 134
BmgBI CAC'GTC
Cuts at: 0 1083 2142
Size: 1083 1059
BmrI ACTGGGnnnn_n'
Cuts at: 0 2130 2142
Size: 2130 12
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 493 1174 2080 2142
Size: 493 681 906 62
Fragments arranged by size:
906 681 493 62
BsaI GGTCTCn'nnnn_
Cuts at: 0 1863 2142
Size: 1863 279
BsaAI yAC'GTr
Cuts at: 0 243 1710 2142
Size: 243 1467 432
Fragments arranged by size:
1467 432 243
BsaBI GATnn'nnATC
Cuts at: 0 1999 2142
Size: 1999 143
BsaHI Gr'CG_yC
Cuts at: 0 43 2142
Size: 43 2099
BsaWI w'CCGG_w
Cuts at: 0 906 1451 1602 1813 1920 2142
Size: 906 545 151 211 107 222
Fragments arranged by size:
906 545 222 211 151 107
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1891 1921 2142
Size: 1891 30 221
Fragments arranged by size:
1891 221 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 490 1912 2142
Size: 490 1422 230
Fragments arranged by size:
1422 490 230
BseYI C'CCAG_C
Cuts at: 0 201 1062 1927 2142
Size: 201 861 865 215
Fragments arranged by size:
865 861 215 201
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 514 674 1030 1321 2142
Size: 223 291 160 356 291 821
Fragments arranged by size:
821 356 291 291 223 160
BsiHKAI G_wGCw'C
Cuts at: 0 527 1100 2142
Size: 527 573 1042
Fragments arranged by size:
1042 573 527
Bsp1286I G_dGCh'C
Cuts at: 0 26 134 214 430 527 986 1033 1100
Size: 26 108 80 216 97 459 47 67
Cuts at: 1100 1239 1610 1625 1960 2142
Size: 139 371 15 335 182
Fragments arranged by size:
459 371 335 216 182 139 108 97
80 67 47 26 15
BspEI T'CCGG_A
Cuts at: 0 1813 2142
Size: 1813 329
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 329 536 1388 2142
Size: 329 207 852 754
Fragments arranged by size:
852 754 329 207
BsrBI CCG'CTC
Cuts at: 0 125 141 2142
Size: 125 16 2001
Fragments arranged by size:
2001 125 16
BsrDI GCAATG_nn'
Cuts at: 0 1938 2142
Size: 1938 204
BsrFI r'CCGG_y
Cuts at: 0 3 36 1982 2142
Size: 3 33 1946 160
Fragments arranged by size:
1946 160 33 3
BssSI C'ACGA_G
Cuts at: 0 1277 2142
Size: 1277 865
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1368 1536 2142
Size: 1368 168 606
Fragments arranged by size:
1368 606 168
BstYI r'GATC_y
Cuts at: 0 338 452 635 651 909 1023 1281 2142
Size: 338 114 183 16 258 114 258 861
Fragments arranged by size:
861 338 258 258 183 114 114 16
Bsu36I CC'TnA_GG
Cuts at: 0 808 1401 2142
Size: 808 593 741
Fragments arranged by size:
808 741 593
BtgI C'CryG_G
Cuts at: 0 83 1550 2142
Size: 83 1467 592
Fragments arranged by size:
1467 592 83
BtsI GCAGTG_nn'
Cuts at: 0 2077 2142
Size: 2077 65
ClaI AT'CG_AT
Cuts at: 0 731 2142
Size: 731 1411
DraIII CAC_nnn'GTG
Cuts at: 0 668 670 2142
Size: 668 2 1472
Fragments arranged by size:
1472 668 2
DrdI GACnn_nn'nnGTC
Cuts at: 0 307 2142
Size: 307 1835
EaeI y'GGCC_r
Cuts at: 0 74 263 1124 2117 2142
Size: 74 189 861 993 25
Fragments arranged by size:
993 861 189 74 25
EarI CTCTTCn'nnn_
Cuts at: 0 280 913 2142
Size: 280 633 1229
Fragments arranged by size:
1229 633 280
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 343 2142
Size: 343 1799
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 493 1174 1465 2080 2142
Size: 493 681 291 615 62
Fragments arranged by size:
681 615 493 291 62
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1444 2142
Size: 1444 698
EcoO109I rG'GnC_Cy
Cuts at: 0 1235 1236 1580 1621 1902 2054 2142
Size: 1235 1 344 41 281 152 88
Fragments arranged by size:
1235 344 281 152 88 41 1
EcoRI G'AATT_C
Cuts at: 0 1288 2142
Size: 1288 854
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1488 2142
Size: 1488 654
FspI TGC'GCA
Cuts at: 0 630 2142
Size: 630 1512
HaeII r_GCGC'y
Cuts at: 0 46 1161 2025 2142
Size: 46 1115 864 117
Fragments arranged by size:
1115 864 117 46
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 494 526 724 756 1266 1298 2142
Size: 494 32 198 32 510 32 844
Fragments arranged by size:
844 510 494 198 32 32 32
HincII GTy'rAC
Cuts at: 0 770 2142
Size: 770 1372
KasI G'GCGC_C
Cuts at: 0 42 2142
Size: 42 2100
MfeI C'AATT_G
Cuts at: 0 1530 1962 2142
Size: 1530 432 180
Fragments arranged by size:
1530 432 180
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 525 1127 1349 1873 2142
Size: 525 602 222 524 269
Fragments arranged by size:
602 525 524 269 222
MscI TGG'CCA
Cuts at: 0 265 1126 2142
Size: 265 861 1016
Fragments arranged by size:
1016 861 265
MslI CAynn'nnrTG
Cuts at: 0 748 1564 2142
Size: 748 816 578
Fragments arranged by size:
816 748 578
MspA1I CmG'CkG
Cuts at: 0 85 2142
Size: 85 2057
NaeI GCC'GGC
Cuts at: 0 5 38 2142
Size: 5 33 2104
Fragments arranged by size:
2104 33 5
NarI GG'CG_CC
Cuts at: 0 43 2142
Size: 43 2099
NcoI C'CATG_G
Cuts at: 0 1550 2142
Size: 1550 592
NgoMIV G'CCGG_C
Cuts at: 0 3 36 2142
Size: 3 33 2106
Fragments arranged by size:
2106 33 3
NruI TCG'CGA
Cuts at: 0 1700 2142
Size: 1700 442
NspI r_CATG'y
Cuts at: 0 759 1824 2142
Size: 759 1065 318
Fragments arranged by size:
1065 759 318
PasI CC'CwG_GG
Cuts at: 0 430 2142
Size: 430 1712
PciI A'CATG_T
Cuts at: 0 755 2142
Size: 755 1387
PflMI CCAn_nnn'nTGG
Cuts at: 0 828 1122 2142
Size: 828 294 1020
Fragments arranged by size:
1020 828 294
PfoI T'CCnGG_A
Cuts at: 0 899 1184 2142
Size: 899 285 958
Fragments arranged by size:
958 899 285
PmlI CAC'GTG
Cuts at: 0 243 2142
Size: 243 1899
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1369 1401 2142
Size: 1369 32 741
Fragments arranged by size:
1369 741 32
PpuMI rG'GwC_Cy
Cuts at: 0 1580 2142
Size: 1580 562
PspOMI G'GGCC_C
Cuts at: 0 1235 1621 2142
Size: 1235 386 521
Fragments arranged by size:
1235 521 386
PstI C_TGCA'G
Cuts at: 0 2039 2049 2084 2142
Size: 2039 10 35 58
Fragments arranged by size:
2039 58 35 10
SacII CC_GC'GG
Cuts at: 0 86 2142
Size: 86 2056
SexAI A'CCwGG_T
Cuts at: 0 410 2142
Size: 410 1732
SfcI C'TryA_G
Cuts at: 0 1737 2035 2045 2080 2142
Size: 1737 298 10 35 62
Fragments arranged by size:
1737 298 62 35 10
SfoI GGC'GCC
Cuts at: 0 44 2142
Size: 44 2098
SmaI CCC'GGG
Cuts at: 0 1802 2142
Size: 1802 340
SspI AAT'ATT
Cuts at: 0 930 1506 2142
Size: 930 576 636
Fragments arranged by size:
930 636 576
StuI AGG'CCT
Cuts at: 0 1258 1459 2142
Size: 1258 201 683
Fragments arranged by size:
1258 683 201
StyI C'CwwG_G
Cuts at: 0 221 1241 1550 2142
Size: 221 1020 309 592
Fragments arranged by size:
1020 592 309 221
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 108 1069 2142
Size: 108 961 1073
Fragments arranged by size:
1073 961 108
TatI w'GTAC_w
Cuts at: 0 1229 2142
Size: 1229 913
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1891 1923 2142
Size: 1891 32 219
Fragments arranged by size:
1891 219 32
Tth111I GACn'n_nGTC
Cuts at: 0 1794 2142
Size: 1794 348
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1947 2142
Size: 1947 195
XmaI C'CCGG_G
Cuts at: 0 1800 2142
Size: 1800 342
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AlfI AlwNI ApaI
ApoI AvaI BamHI BanI BanII BbeI BbsI BcgI
BclI BglI BglII Bme1580I BmgBI BmrI BpmI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BssSI
BstXI BstYI Bsu36I BtgI BtsI ClaI DraIII DrdI
EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI MfeI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NruI NspI
PasI PciI PflMI PfoI PmlI PpiI PpuMI PspOMI
PstI SacII SexAI SfcI SfoI SmaI SspI StuI
StyI TaqII TatI TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AhdI AleI AloI
ApaLI AscI AseI AsiSI AvrII BaeI BbvCI BciVI
BfrBI BlpI BmtI BplI Bpu10I BpuEI BsiEI BsiWI
BsmI BsmBI BspHI BsrGI BssHII BstAPI BstBI BstEII
BstZ17I BtgZI CspCI DraI EagI EcoICRI EcoRV FseI
FspAI HindIII HpaI KpnI MluI NdeI NheI NotI
NsiI PacI PmeI PshAI PsiI PspXI PsrI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfiI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI
SwaI XbaI XhoI XmnI ZraI