Restriction map for
pFN21AE3277
(Linear) (Six-base) MAPSORT of: KIEE3277.seq Check: 8936 from: 1 to: 2076
KIEE3277.orf
With 182 enzymes: SgfI *
July 3, 2012 17:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1329 1452 2076
Size: 1329 123 624
Fragments arranged by size:
1329 624 123
AccI GT'mk_AC
Cuts at: 0 711 2076
Size: 711 1365
Acc65I G'GTAC_C
Cuts at: 0 493 2076
Size: 493 1583
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 658 2076
Size: 658 1418
AfeI AGC'GCT
Cuts at: 0 283 2076
Size: 283 1793
AgeI A'CCGG_T
Cuts at: 0 674 2076
Size: 674 1402
AleI CACnn'nnGTG
Cuts at: 0 487 802 997 1306 2076
Size: 487 315 195 309 770
Fragments arranged by size:
770 487 315 309 195
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 988 2076
Size: 988 1088
AlwNI CAG_nnn'CTG
Cuts at: 0 84 336 1723 2076
Size: 84 252 1387 353
Fragments arranged by size:
1387 353 252 84
ApaI G_GGCC'C
Cuts at: 0 564 2076
Size: 564 1512
ApaLI G'TGCA_C
Cuts at: 0 52 355 2076
Size: 52 303 1721
Fragments arranged by size:
1721 303 52
AvaI C'yCGr_G
Cuts at: 0 896 1734 2076
Size: 896 838 342
Fragments arranged by size:
896 838 342
AvrII C'CTAG_G
Cuts at: 0 1080 1266 2076
Size: 1080 186 810
Fragments arranged by size:
1080 810 186
BamHI G'GATC_C
Cuts at: 0 17 2076
Size: 17 2059
BanI G'GyrC_C
Cuts at: 0 37 493 652 964 1262 1432 1464 1780
Size: 37 456 159 312 298 170 32 316
Cuts at: 1780 1806 2076
Size: 26 270
Fragments arranged by size:
456 316 312 298 270 170 159 37
32 26
BanII G_rGCy'C
Cuts at: 0 564 2076
Size: 564 1512
BbsI GAAGACnn'nnnn_
Cuts at: 0 221 2076
Size: 221 1855
BciVI GTATCCnnnnn_n'
Cuts at: 0 901 2076
Size: 901 1175
BclI T'GATC_A
Cuts at: 0 89 2076
Size: 89 1987
BglI GCCn_nnn'nGGC
Cuts at: 0 1351 1832 2076
Size: 1351 481 244
Fragments arranged by size:
1351 481 244
BlpI GC'TnA_GC
Cuts at: 0 1490 2076
Size: 1490 586
Bme1580I G_kGCm'C
Cuts at: 0 56 359 564 783 967 1469 1708 1783
Size: 56 303 205 219 184 502 239 75
Cuts at: 1783 1809 2076
Size: 26 267
Fragments arranged by size:
502 303 267 239 219 205 184 75
56 26
BmgBI CAC'GTC
Cuts at: 0 804 2076
Size: 804 1272
BmrI ACTGGGnnnn_n'
Cuts at: 0 1128 1432 2076
Size: 1128 304 644
Fragments arranged by size:
1128 644 304
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1117 2076
Size: 1117 959
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 319 742 1174 2076
Size: 319 423 432 902
Fragments arranged by size:
902 432 423 319
Bpu10I CC'TnA_GC
Cuts at: 0 460 515 686 1642 2076
Size: 460 55 171 956 434
Fragments arranged by size:
956 460 434 171 55
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 370 580 1849 2076
Size: 148 222 210 1269 227
Fragments arranged by size:
1269 227 222 210 148
BsaI GGTCTCn'nnnn_
Cuts at: 0 301 374 2076
Size: 301 73 1702
Fragments arranged by size:
1702 301 73
BsaAI yAC'GTr
Cuts at: 0 1185 1362 2076
Size: 1185 177 714
Fragments arranged by size:
1185 714 177
BsaHI Gr'CG_yC
Cuts at: 0 1037 1053 2076
Size: 1037 16 1023
Fragments arranged by size:
1037 1023 16
BsaWI w'CCGG_w
Cuts at: 0 674 2076
Size: 674 1402
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1147 1177 1579 1609 2076
Size: 1147 30 402 30 467
Fragments arranged by size:
1147 467 402 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 316 319 775 1621 1648 2076
Size: 316 3 456 846 27 428
Fragments arranged by size:
846 456 428 316 27 3
BseYI C'CCAG_C
Cuts at: 0 731 847 960 1015 1320 1457 1578 2076
Size: 731 116 113 55 305 137 121 498
Fragments arranged by size:
731 498 305 137 121 116 113 55
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 301 349 358 1106 1357 1649 1993 2076
Size: 301 48 9 748 251 292 344 83
Fragments arranged by size:
748 344 301 292 251 83 48 9
BsiEI CG_ry'CG
Cuts at: 0 2050 2076
Size: 2050 26
BsiHKAI G_wGCw'C
Cuts at: 0 56 359 2076
Size: 56 303 1717
Fragments arranged by size:
1717 303 56
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1060 2076
Size: 1060 1016
Bsp1286I G_dGCh'C
Cuts at: 0 56 359 564 783 967 1469 1708 1783
Size: 56 303 205 219 184 502 239 75
Cuts at: 1783 1809 2076
Size: 26 267
Fragments arranged by size:
502 303 267 239 219 205 184 75
56 26
BspHI T'CATG_A
Cuts at: 0 644 1235 2076
Size: 644 591 841
Fragments arranged by size:
841 644 591
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1329 1452 2076
Size: 1329 123 624
Fragments arranged by size:
1329 624 123
BsrBI CCG'CTC
Cuts at: 0 15 132 2076
Size: 15 117 1944
Fragments arranged by size:
1944 117 15
BsrDI GCAATG_nn'
Cuts at: 0 1263 1853 2076
Size: 1263 590 223
Fragments arranged by size:
1263 590 223
BsrFI r'CCGG_y
Cuts at: 0 674 1033 2064 2076
Size: 674 359 1031 12
Fragments arranged by size:
1031 674 359 12
BstAPI GCAn_nnn'nTGC
Cuts at: 0 336 354 1262 2076
Size: 336 18 908 814
Fragments arranged by size:
908 814 336 18
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1308 2076
Size: 1308 768
BstYI r'GATC_y
Cuts at: 0 17 332 455 2076
Size: 17 315 123 1621
Fragments arranged by size:
1621 315 123 17
BtgI C'CryG_G
Cuts at: 0 236 839 1272 1404 2022 2076
Size: 236 603 433 132 618 54
Fragments arranged by size:
618 603 433 236 132 54
BtsI GCAGTG_nn'
Cuts at: 0 343 816 1121 1205 1706 2076
Size: 343 473 305 84 501 370
Fragments arranged by size:
501 473 370 343 305 84
ClaI AT'CG_AT
Cuts at: 0 1511 2076
Size: 1511 565
DrdI GACnn_nn'nnGTC
Cuts at: 0 2010 2076
Size: 2010 66
EaeI y'GGCC_r
Cuts at: 0 587 736 1539 1966 2076
Size: 587 149 803 427 110
Fragments arranged by size:
803 587 427 149 110
EarI CTCTTCn'nnn_
Cuts at: 0 122 541 2076
Size: 122 419 1535
Fragments arranged by size:
1535 419 122
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1106 2076
Size: 1106 970
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 319 658 742 1174 2076
Size: 319 339 84 432 902
Fragments arranged by size:
902 432 339 319 84
EcoNI CCTnn'n_nnAGG
Cuts at: 0 67 298 1066 1603 1675 2076
Size: 67 231 768 537 72 401
Fragments arranged by size:
768 537 401 231 72 67
EcoO109I rG'GnC_Cy
Cuts at: 0 159 561 901 1071 1352 1638 2076
Size: 159 402 340 170 281 286 438
Fragments arranged by size:
438 402 340 286 281 170 159
HaeII r_GCGC'y
Cuts at: 0 285 2076
Size: 285 1791
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1147 1179 1217 1249 1579 1611 2076
Size: 1147 32 38 32 330 32 465
Fragments arranged by size:
1147 465 330 38 32 32 32
KpnI G_GTAC'C
Cuts at: 0 497 2076
Size: 497 1579
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1958 2076
Size: 1958 118
MscI TGG'CCA
Cuts at: 0 589 1541 2076
Size: 589 952 535
Fragments arranged by size:
952 589 535
MslI CAynn'nnrTG
Cuts at: 0 182 487 802 997 1234 1306 1840 2076
Size: 182 305 315 195 237 72 534 236
Fragments arranged by size:
534 315 305 237 236 195 182 72
MspA1I CmG'CkG
Cuts at: 0 699 735 881 1015 1250 1415 1451 1720
Size: 699 36 146 134 235 165 36 269
Cuts at: 1720 2076
Size: 356
Fragments arranged by size:
699 356 269 235 165 146 134 36
36
NaeI GCC'GGC
Cuts at: 0 1035 2076
Size: 1035 1041
NcoI C'CATG_G
Cuts at: 0 236 1272 2022 2076
Size: 236 1036 750 54
Fragments arranged by size:
1036 750 236 54
NgoMIV G'CCGG_C
Cuts at: 0 1033 2076
Size: 1033 1043
NspI r_CATG'y
Cuts at: 0 822 2076
Size: 822 1254
PasI CC'CwG_GG
Cuts at: 0 564 1599 2076
Size: 564 1035 477
Fragments arranged by size:
1035 564 477
PflMI CCAn_nnn'nTGG
Cuts at: 0 84 756 1629 2076
Size: 84 672 873 447
Fragments arranged by size:
873 672 447 84
PfoI T'CCnGG_A
Cuts at: 0 209 1151 2076
Size: 209 942 925
Fragments arranged by size:
942 925 209
PmlI CAC'GTG
Cuts at: 0 1185 1362 2076
Size: 1185 177 714
Fragments arranged by size:
1185 714 177
PpuMI rG'GwC_Cy
Cuts at: 0 159 901 2076
Size: 159 742 1175
Fragments arranged by size:
1175 742 159
PspOMI G'GGCC_C
Cuts at: 0 560 2076
Size: 560 1516
PstI C_TGCA'G
Cuts at: 0 886 2076
Size: 886 1190
PvuI CG_AT'CG
Cuts at: 0 2050 2076
Size: 2050 26
PvuII CAG'CTG
Cuts at: 0 699 1015 1451 1720 2076
Size: 699 316 436 269 356
Fragments arranged by size:
699 436 356 316 269
SapI GCTCTTCn'nnn_
Cuts at: 0 122 2076
Size: 122 1954
ScaI AGT'ACT
Cuts at: 0 196 2076
Size: 196 1880
SfcI C'TryA_G
Cuts at: 0 882 1874 2076
Size: 882 992 202
Fragments arranged by size:
992 882 202
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1351 2076
Size: 1351 725
SmaI CCC'GGG
Cuts at: 0 898 2076
Size: 898 1178
SmlI C'TyrA_G
Cuts at: 0 163 385 595 1828 2076
Size: 163 222 210 1233 248
Fragments arranged by size:
1233 248 222 210 163
SphI G_CATG'C
Cuts at: 0 822 2076
Size: 822 1254
StuI AGG'CCT
Cuts at: 0 478 1826 2076
Size: 478 1348 250
Fragments arranged by size:
1348 478 250
StyI C'CwwG_G
Cuts at: 0 122 236 1080 1266 1272 1821 2022 2076
Size: 122 114 844 186 6 549 201 54
Fragments arranged by size:
844 549 201 186 122 114 54 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 376 1250 2040 2076
Size: 376 874 790 36
Fragments arranged by size:
874 790 376 36
TatI w'GTAC_w
Cuts at: 0 194 1901 2076
Size: 194 1707 175
Fragments arranged by size:
1707 194 175
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 858 890 2076
Size: 858 32 1186
Fragments arranged by size:
1186 858 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1012 1182 2076
Size: 1012 170 894
Fragments arranged by size:
1012 894 170
XmaI C'CCGG_G
Cuts at: 0 896 2076
Size: 896 1180
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AgeI AleI AlfI
AlwNI ApaI ApaLI AvaI AvrII BamHI BanI BanII
BbsI BciVI BclI BglI BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BstAPI BstXI BstYI
BtgI BtsI ClaI DrdI EaeI EarI EciI Eco57MI
EcoNI EcoO109I HaeII Hin4I KpnI MmeI MscI MslI
MspA1I NaeI NcoI NgoMIV NspI PasI PflMI PfoI
PmlI PpuMI PspOMI PstI PvuI PvuII SapI ScaI
SfcI SfiI SmaI SmlI SphI StuI StyI TaqII
TatI TstI XcmI XmaI
Enzymes that do not cut:
AatII AclI AflII AflIII AhdI AloI ApoI AscI
AseI AsiSI BaeI BbeI BbvCI BcgI BfrBI BglII
BmtI BsaBI BsiWI BsmI BspEI BsrGI BssHII BssSI
BstBI BstEII BstZ17I Bsu36I BtgZI CspCI DraI DraIII
EagI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KasI MfeI MluI NarI NdeI
NheI NotI NruI NsiI PacI PciI PmeI PpiI
PshAI PsiI PspXI PsrI RsrII SacI SacII SalI
SanDI SbfI SexAI SfoI SgfI SgrAI SnaBI SpeI
SrfI SspI SwaI Tth111I XbaI XhoI XmnI ZraI