Restriction map for
pFN21AE3270
(Linear) (Six-base) MAPSORT of: KIEE3270.seq Check: 355 from: 1 to: 2019
KIEE3270.orf
With 182 enzymes: SgfI *
December 27, 2011 16:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 753 1229 2019
Size: 753 476 790
Fragments arranged by size:
790 753 476
Acc65I G'GTAC_C
Cuts at: 0 1568 2019
Size: 1568 451
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1460 1530 2019
Size: 1460 70 489
Fragments arranged by size:
1460 489 70
AhdI GACnn_n'nnGTC
Cuts at: 0 1074 2019
Size: 1074 945
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1405 1437 2019
Size: 1405 32 582
Fragments arranged by size:
1405 582 32
AlwNI CAG_nnn'CTG
Cuts at: 0 345 380 672 810 1164 2019
Size: 345 35 292 138 354 855
Fragments arranged by size:
855 354 345 292 138 35
ApaI G_GGCC'C
Cuts at: 0 791 1353 2019
Size: 791 562 666
Fragments arranged by size:
791 666 562
ApaLI G'TGCA_C
Cuts at: 0 800 1057 2019
Size: 800 257 962
Fragments arranged by size:
962 800 257
ApoI r'AATT_y
Cuts at: 0 855 2019
Size: 855 1164
AvaI C'yCGr_G
Cuts at: 0 107 461 784 1283 1482 2019
Size: 107 354 323 499 199 537
Fragments arranged by size:
537 499 354 323 199 107
BamHI G'GATC_C
Cuts at: 0 414 1540 2019
Size: 414 1126 479
Fragments arranged by size:
1126 479 414
BanI G'GyrC_C
Cuts at: 0 1568 1901 2019
Size: 1568 333 118
Fragments arranged by size:
1568 333 118
BanII G_rGCy'C
Cuts at: 0 517 697 727 791 940 968 977 1353
Size: 517 180 30 64 149 28 9 376
Cuts at: 1353 1815 2019
Size: 462 204
Fragments arranged by size:
517 462 376 204 180 149 64 30
28 9
BbsI GAAGACnn'nnnn_
Cuts at: 0 492 1262 2019
Size: 492 770 757
Fragments arranged by size:
770 757 492
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 277 311 2019
Size: 277 34 1708
Fragments arranged by size:
1708 277 34
BfrBI ATG'CAT
Cuts at: 0 194 2019
Size: 194 1825
BglI GCCn_nnn'nGGC
Cuts at: 0 292 457 521 2019
Size: 292 165 64 1498
Fragments arranged by size:
1498 292 165 64
BglII A'GATC_T
Cuts at: 0 359 1262 2019
Size: 359 903 757
Fragments arranged by size:
903 757 359
BlpI GC'TnA_GC
Cuts at: 0 617 775 2019
Size: 617 158 1244
Fragments arranged by size:
1244 617 158
Bme1580I G_kGCm'C
Cuts at: 0 791 804 1061 1353 2019
Size: 791 13 257 292 666
Fragments arranged by size:
791 666 292 257 13
BmrI ACTGGGnnnn_n'
Cuts at: 0 499 1060 1396 1907 2019
Size: 499 561 336 511 112
Fragments arranged by size:
561 511 499 336 112
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 489 1497 2019
Size: 489 1008 522
Fragments arranged by size:
1008 522 489
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 655 739 891 982 1906 2019
Size: 205 450 84 152 91 924 113
Fragments arranged by size:
924 450 205 152 113 91 84
Bpu10I CC'TnA_GC
Cuts at: 0 511 934 2019
Size: 511 423 1085
Fragments arranged by size:
1085 511 423
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 898 1048 2019
Size: 898 150 971
Fragments arranged by size:
971 898 150
BsaI GGTCTCn'nnnn_
Cuts at: 0 739 1742 2019
Size: 739 1003 277
Fragments arranged by size:
1003 739 277
BsaAI yAC'GTr
Cuts at: 0 828 2019
Size: 828 1191
BsaWI w'CCGG_w
Cuts at: 0 43 641 2019
Size: 43 598 1378
Fragments arranged by size:
1378 598 43
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 628 658 824 854 1405 1435 1802 1818
Size: 628 30 166 30 551 30 367 16
Cuts at: 1818 1832 1848 2019
Size: 14 16 171
Fragments arranged by size:
628 551 367 171 166 30 30 30
16 16 14
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1570 2019
Size: 1570 449
BseYI C'CCAG_C
Cuts at: 0 100 269 419 755 2019
Size: 100 169 150 336 1264
Fragments arranged by size:
1264 336 169 150 100
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 781 1105 1201 1603 2019
Size: 781 324 96 402 416
Fragments arranged by size:
781 416 402 324 96
BsiEI CG_ry'CG
Cuts at: 0 1116 2019
Size: 1116 903
BsiHKAI G_wGCw'C
Cuts at: 0 804 968 977 1061 1815 2019
Size: 804 164 9 84 754 204
Fragments arranged by size:
804 754 204 164 84 9
BsmI GAATG_Cn'
Cuts at: 0 43 2019
Size: 43 1976
BsmBI CGTCTCn'nnnn_
Cuts at: 0 342 595 2019
Size: 342 253 1424
Fragments arranged by size:
1424 342 253
Bsp1286I G_dGCh'C
Cuts at: 0 517 697 727 791 804 940 968 977
Size: 517 180 30 64 13 136 28 9
Cuts at: 977 1061 1353 1815 2019
Size: 84 292 462 204
Fragments arranged by size:
517 462 292 204 180 136 84 64
30 28 13 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 680 753 1157 1229 1580 2019
Size: 680 73 404 72 351 439
Fragments arranged by size:
680 439 404 351 73 72
BsrFI r'CCGG_y
Cuts at: 0 65 2019
Size: 65 1954
BssSI C'ACGA_G
Cuts at: 0 229 2019
Size: 229 1790
BstAPI GCAn_nnn'nTGC
Cuts at: 0 224 2019
Size: 224 1795
BstEII G'GTnAC_C
Cuts at: 0 1635 2019
Size: 1635 384
BstXI CCAn_nnnn'nTGG
Cuts at: 0 141 2019
Size: 141 1878
BstYI r'GATC_y
Cuts at: 0 359 414 1209 1262 1540 2019
Size: 359 55 795 53 278 479
Fragments arranged by size:
795 479 359 278 55 53
Bsu36I CC'TnA_GG
Cuts at: 0 398 2019
Size: 398 1621
BtgI C'CryG_G
Cuts at: 0 455 1506 2019
Size: 455 1051 513
Fragments arranged by size:
1051 513 455
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1808 2019
Size: 1808 211
BtsI GCAGTG_nn'
Cuts at: 0 1677 1740 2019
Size: 1677 63 279
Fragments arranged by size:
1677 279 63
EaeI y'GGCC_r
Cuts at: 0 257 1113 2019
Size: 257 856 906
Fragments arranged by size:
906 856 257
EagI C'GGCC_G
Cuts at: 0 1113 2019
Size: 1113 906
EarI CTCTTCn'nnn_
Cuts at: 0 504 1249 1768 2019
Size: 504 745 519 251
Fragments arranged by size:
745 519 504 251
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 273 2019
Size: 273 1746
EcoICRI GAG'CTC
Cuts at: 0 966 975 1813 2019
Size: 966 9 838 206
Fragments arranged by size:
966 838 206 9
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 655 739 891 982 1460 1530 1906
Size: 205 450 84 152 91 478 70 376
Cuts at: 1906 2019
Size: 113
Fragments arranged by size:
478 450 376 205 152 113 91 84
70
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1084 2019
Size: 1084 935
EcoO109I rG'GnC_Cy
Cuts at: 0 669 788 885 954 1068 1350 1801 2019
Size: 669 119 97 69 114 282 451 218
Fragments arranged by size:
669 451 282 218 119 114 97 69
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1671 2019
Size: 1671 348
FseI GG_CCGG'CC
Cuts at: 0 69 2019
Size: 69 1950
HaeII r_GCGC'y
Cuts at: 0 1202 1589 2019
Size: 1202 387 430
Fragments arranged by size:
1202 430 387
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 628 660 1060 1092 1439 1471 1802 1834
Size: 628 32 400 32 347 32 331 32
Cuts at: 1834 1975 2007 2019
Size: 141 32 12
Fragments arranged by size:
628 400 347 331 141 32 32 32
32 32 12
KpnI G_GTAC'C
Cuts at: 0 1572 2019
Size: 1572 447
MscI TGG'CCA
Cuts at: 0 259 2019
Size: 259 1760
MslI CAynn'nnrTG
Cuts at: 0 826 868 2019
Size: 826 42 1151
Fragments arranged by size:
1151 826 42
MspA1I CmG'CkG
Cuts at: 0 214 269 755 849 1407 1667 1709 1847
Size: 214 55 486 94 558 260 42 138
Cuts at: 1847 2019
Size: 172
Fragments arranged by size:
558 486 260 214 172 138 94 55
42
NaeI GCC'GGC
Cuts at: 0 67 2019
Size: 67 1952
NcoI C'CATG_G
Cuts at: 0 1506 2019
Size: 1506 513
NgoMIV G'CCGG_C
Cuts at: 0 65 2019
Size: 65 1954
NsiI A_TGCA'T
Cuts at: 0 196 2019
Size: 196 1823
NspI r_CATG'y
Cuts at: 0 98 243 1399 2019
Size: 98 145 1156 620
Fragments arranged by size:
1156 620 145 98
PfoI T'CCnGG_A
Cuts at: 0 959 2019
Size: 959 1060
PmlI CAC'GTG
Cuts at: 0 828 2019
Size: 828 1191
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1405 1437 1443 1475 2019
Size: 1405 32 6 32 544
Fragments arranged by size:
1405 544 32 32 6
PpuMI rG'GwC_Cy
Cuts at: 0 669 1068 1801 2019
Size: 669 399 733 218
Fragments arranged by size:
733 669 399 218
PspOMI G'GGCC_C
Cuts at: 0 787 1349 2019
Size: 787 562 670
Fragments arranged by size:
787 670 562
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1129 1161 2019
Size: 1129 32 858
Fragments arranged by size:
1129 858 32
PstI C_TGCA'G
Cuts at: 0 169 359 854 1160 1198 1672 1747 2019
Size: 169 190 495 306 38 474 75 272
Fragments arranged by size:
495 474 306 272 190 169 75 38
PvuII CAG'CTG
Cuts at: 0 214 269 849 1407 1667 1847 2019
Size: 214 55 580 558 260 180 172
Fragments arranged by size:
580 558 260 214 180 172 55
SacI G_AGCT'C
Cuts at: 0 968 977 1815 2019
Size: 968 9 838 204
Fragments arranged by size:
968 838 204 9
SexAI A'CCwGG_T
Cuts at: 0 368 1070 1751 2019
Size: 368 702 681 268
Fragments arranged by size:
702 681 368 268
SfcI C'TryA_G
Cuts at: 0 165 355 793 850 1156 1194 1668 1743
Size: 165 190 438 57 306 38 474 75
Cuts at: 1743 2019
Size: 276
Fragments arranged by size:
474 438 306 276 190 165 75 57
38
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 457 2019
Size: 457 1562
SmaI CCC'GGG
Cuts at: 0 463 786 1285 2019
Size: 463 323 499 734
Fragments arranged by size:
734 499 463 323
SmlI C'TyrA_G
Cuts at: 0 913 1063 2019
Size: 913 150 956
Fragments arranged by size:
956 913 150
StuI AGG'CCT
Cuts at: 0 557 2019
Size: 557 1462
StyI C'CwwG_G
Cuts at: 0 950 1176 1506 1787 2019
Size: 950 226 330 281 232
Fragments arranged by size:
950 330 281 232 226
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 261 352 747 1380 1532 1889 2019
Size: 261 91 395 633 152 357 130
Fragments arranged by size:
633 395 357 261 152 130 91
TatI w'GTAC_w
Cuts at: 0 1047 2019
Size: 1047 972
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 824 856 1818 1850 2019
Size: 824 32 962 32 169
Fragments arranged by size:
962 824 169 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1378 2019
Size: 1378 641
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 957 1605 1846 1946 2019
Size: 957 648 241 100 73
Fragments arranged by size:
957 648 241 100 73
XmaI C'CCGG_G
Cuts at: 0 461 784 1283 2019
Size: 461 323 499 736
Fragments arranged by size:
736 499 461 323
Enzymes that do cut:
AarI Acc65I AcuI AhdI AloI AlwNI ApaI ApaLI
ApoI AvaI BamHI BanI BanII BbsI BcgI BfrBI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrFI BssSI
BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
FalI FseI HaeII Hin4I KpnI MscI MslI MspA1I
NaeI NcoI NgoMIV NsiI NspI PfoI PmlI PpiI
PpuMI PspOMI PsrI PstI PvuII SacI SexAI SfcI
SfiI SmaI SmlI StuI StyI TaqII TatI TstI
Tth111I XcmI XmaI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AleI
AlfI AscI AseI AsiSI AvrII BaeI BbeI BbvCI
BciVI BclI BmgBI BmtI BsaBI BsaHI BsiWI BspEI
BspHI BsrBI BsrDI BsrGI BssHII BstBI BstZ17I ClaI
CspCI DraI DraIII DrdI EcoRI EcoRV FspI FspAI
HincII HindIII HpaI KasI MfeI MluI MmeI NarI
NdeI NheI NotI NruI PacI PasI PciI PflMI
PmeI PshAI PsiI PspXI PvuI RsrII SacII SalI
SanDI SapI SbfI ScaI SfoI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI SwaI XbaI XhoI XmnI
ZraI