Restriction map for
pFN21AE3265
(Linear) (Six-base) MAPSORT of: KIEE3265.seq Check: 4074 from: 1 to: 1932
KIEE3265.orf
With 182 enzymes: SgfI *
July 3, 2012 17:32 ..
AclI AA'CG_TT
Cuts at: 0 1530 1932
Size: 1530 402
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 956 1932
Size: 956 976
AflIII A'CryG_T
Cuts at: 0 175 531 1932
Size: 175 356 1401
Fragments arranged by size:
1401 356 175
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 46 78 1932
Size: 46 32 1854
Fragments arranged by size:
1854 46 32
AlwNI CAG_nnn'CTG
Cuts at: 0 949 1932
Size: 949 983
ApoI r'AATT_y
Cuts at: 0 297 1255 1932
Size: 297 958 677
Fragments arranged by size:
958 677 297
AvaI C'yCGr_G
Cuts at: 0 1052 1932
Size: 1052 880
BamHI G'GATC_C
Cuts at: 0 844 1932
Size: 844 1088
BanI G'GyrC_C
Cuts at: 0 1575 1932
Size: 1575 357
BanII G_rGCy'C
Cuts at: 0 511 1932
Size: 511 1421
BbvCI CC'TCA_GC
Cuts at: 0 1784 1932
Size: 1784 148
BfrBI ATG'CAT
Cuts at: 0 1474 1932
Size: 1474 458
Bme1580I G_kGCm'C
Cuts at: 0 1580 1932
Size: 1580 352
BmrI ACTGGGnnnn_n'
Cuts at: 0 986 1932
Size: 986 946
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 56 1932
Size: 56 1876
Bpu10I CC'TnA_GC
Cuts at: 0 249 1784 1932
Size: 249 1535 148
Fragments arranged by size:
1535 249 148
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 360 1932
Size: 360 1572
BsaWI w'CCGG_w
Cuts at: 0 1889 1932
Size: 1889 43
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 46 76 972 1002 1932
Size: 46 30 896 30 930
Fragments arranged by size:
930 896 46 30 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 212 1932
Size: 212 1720
BsiHKAI G_wGCw'C
Cuts at: 0 1059 1932
Size: 1059 873
Bsp1286I G_dGCh'C
Cuts at: 0 511 1059 1580 1932
Size: 511 548 521 352
Fragments arranged by size:
548 521 511 352
BspEI T'CCGG_A
Cuts at: 0 1889 1932
Size: 1889 43
BsrBI CCG'CTC
Cuts at: 0 1131 1932
Size: 1131 801
BssSI C'ACGA_G
Cuts at: 0 1058 1932
Size: 1058 874
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1878 1932
Size: 1878 54
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1587 1932
Size: 1587 345
BstYI r'GATC_y
Cuts at: 0 760 844 1910 1932
Size: 760 84 1066 22
Fragments arranged by size:
1066 760 84 22
BtgI C'CryG_G
Cuts at: 0 1652 1932
Size: 1652 280
BtsI GCAGTG_nn'
Cuts at: 0 417 1529 1932
Size: 417 1112 403
Fragments arranged by size:
1112 417 403
DraI TTT'AAA
Cuts at: 0 705 1932
Size: 705 1227
EaeI y'GGCC_r
Cuts at: 0 270 285 1932
Size: 270 15 1647
Fragments arranged by size:
1647 270 15
EarI CTCTTCn'nnn_
Cuts at: 0 542 869 1932
Size: 542 327 1063
Fragments arranged by size:
1063 542 327
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 56 956 1932
Size: 56 900 976
Fragments arranged by size:
976 900 56
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 212 338 491 1932
Size: 212 126 153 1441
Fragments arranged by size:
1441 212 153 126
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 736 768 972 1004 1932
Size: 736 32 204 32 928
Fragments arranged by size:
928 736 204 32 32
HincII GTy'rAC
Cuts at: 0 1563 1932
Size: 1563 369
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1009 1513 1932
Size: 1009 504 419
Fragments arranged by size:
1009 504 419
MscI TGG'CCA
Cuts at: 0 272 287 1932
Size: 272 15 1645
Fragments arranged by size:
1645 272 15
MslI CAynn'nnrTG
Cuts at: 0 422 1169 1426 1932
Size: 422 747 257 506
Fragments arranged by size:
747 506 422 257
MspA1I CmG'CkG
Cuts at: 0 588 792 1932
Size: 588 204 1140
Fragments arranged by size:
1140 588 204
NcoI C'CATG_G
Cuts at: 0 1652 1932
Size: 1652 280
NdeI CA'TA_TG
Cuts at: 0 435 1932
Size: 435 1497
NsiI A_TGCA'T
Cuts at: 0 1476 1932
Size: 1476 456
NspI r_CATG'y
Cuts at: 0 179 535 1474 1881 1932
Size: 179 356 939 407 51
Fragments arranged by size:
939 407 356 179 51
PciI A'CATG_T
Cuts at: 0 175 531 1932
Size: 175 356 1401
Fragments arranged by size:
1401 356 175
PflMI CCAn_nnn'nTGG
Cuts at: 0 1748 1932
Size: 1748 184
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 46 78 1932
Size: 46 32 1854
Fragments arranged by size:
1854 46 32
PsiI TTA'TAA
Cuts at: 0 200 1932
Size: 200 1732
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1127 1159 1932
Size: 1127 32 773
Fragments arranged by size:
1127 773 32
PstI C_TGCA'G
Cuts at: 0 1681 1932
Size: 1681 251
PvuII CAG'CTG
Cuts at: 0 588 792 1932
Size: 588 204 1140
Fragments arranged by size:
1140 588 204
SfcI C'TryA_G
Cuts at: 0 592 620 1677 1932
Size: 592 28 1057 255
Fragments arranged by size:
1057 592 255 28
SmlI C'TyrA_G
Cuts at: 0 375 1052 1932
Size: 375 677 880
Fragments arranged by size:
880 677 375
SpeI A'CTAG_T
Cuts at: 0 1036 1932
Size: 1036 896
SphI G_CATG'C
Cuts at: 0 1474 1932
Size: 1474 458
SspI AAT'ATT
Cuts at: 0 1635 1932
Size: 1635 297
StyI C'CwwG_G
Cuts at: 0 1652 1932
Size: 1652 280
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 179 1645 1932
Size: 179 1466 287
Fragments arranged by size:
1466 287 179
TatI w'GTAC_w
Cuts at: 0 538 1143 1593 1932
Size: 538 605 450 339
Fragments arranged by size:
605 538 450 339
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 627 659 1932
Size: 627 32 1273
Fragments arranged by size:
1273 627 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 280 473 1932
Size: 280 193 1459
Fragments arranged by size:
1459 280 193
XhoI C'TCGA_G
Cuts at: 0 1052 1932
Size: 1052 880
XmnI GAAnn'nnTTC
Cuts at: 0 1340 1932
Size: 1340 592
Enzymes that do cut:
AclI AcuI AflIII AloI AlwNI ApoI AvaI BamHI
BanI BanII BbvCI BfrBI Bme1580I BmrI BpmI Bpu10I
BpuEI BsaWI BsaXI BsgI BsiHKAI Bsp1286I BspEI BsrBI
BssSI BstAPI BstXI BstYI BtgI BtsI DraI EaeI
EarI Eco57MI FalI Hin4I HincII MmeI MscI MslI
MspA1I NcoI NdeI NsiI NspI PciI PflMI PpiI
PsiI PsrI PstI PvuII SfcI SmlI SpeI SphI
SspI StyI TaqII TatI TstI XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AgeI AhdI
AleI AlfI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI BmgBI BmtI BplI BsaI BsaAI BsaBI BsaHI
BseRI BseYI BsiEI BsiWI BsmI BsmBI BspHI BspMI
BsrDI BsrFI BsrGI BssHII BstBI BstEII BstZ17I Bsu36I
BtgZI ClaI CspCI DraIII DrdI EagI EciI EcoICRI
EcoNI EcoO109I EcoRI EcoRV FseI FspI FspAI HaeII
HindIII HpaI KasI KpnI MfeI MluI NaeI NarI
NgoMIV NheI NotI NruI PacI PasI PfoI PmeI
PmlI PpuMI PshAI PspOMI PspXI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SrfI StuI SwaI
Tth111I XbaI XmaI ZraI