Restriction map for
pFN21AE3261
(Linear) (Six-base) MAPSORT of: KIEE3261.seq Check: 6846 from: 1 to: 1878
KIEE3261.orf
With 182 enzymes: SgfI *
March 22, 2012 16:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 404 1878
Size: 404 1474
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1318 1878
Size: 1318 560
AfeI AGC'GCT
Cuts at: 0 550 1878
Size: 550 1328
AleI CACnn'nnGTG
Cuts at: 0 1426 1878
Size: 1426 452
AlwNI CAG_nnn'CTG
Cuts at: 0 116 1878
Size: 116 1762
ApaI G_GGCC'C
Cuts at: 0 183 239 1864 1878
Size: 183 56 1625 14
Fragments arranged by size:
1625 183 56 14
ApoI r'AATT_y
Cuts at: 0 325 1054 1244 1518 1878
Size: 325 729 190 274 360
Fragments arranged by size:
729 360 325 274 190
AvaI C'yCGr_G
Cuts at: 0 254 872 1380 1592 1878
Size: 254 618 508 212 286
Fragments arranged by size:
618 508 286 254 212
BanI G'GyrC_C
Cuts at: 0 6 1363 1878
Size: 6 1357 515
Fragments arranged by size:
1357 515 6
BanII G_rGCy'C
Cuts at: 0 62 183 239 622 758 879 1864 1878
Size: 62 121 56 383 136 121 985 14
Fragments arranged by size:
985 383 136 121 121 62 56 14
BbeI G_GCGC'C
Cuts at: 0 10 1878
Size: 10 1868
BbsI GAAGACnn'nnnn_
Cuts at: 0 270 1878
Size: 270 1608
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 791 825 1878
Size: 791 34 1053
Fragments arranged by size:
1053 791 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 757 1878
Size: 757 1121
BlpI GC'TnA_GC
Cuts at: 0 1030 1571 1878
Size: 1030 541 307
Fragments arranged by size:
1030 541 307
Bme1580I G_kGCm'C
Cuts at: 0 100 136 183 239 495 1864 1873 1878
Size: 100 36 47 56 256 1369 9 5
Fragments arranged by size:
1369 256 100 56 47 36 9 5
BmgBI CAC'GTC
Cuts at: 0 168 1614 1878
Size: 168 1446 264
Fragments arranged by size:
1446 264 168
BmrI ACTGGGnnnn_n'
Cuts at: 0 451 588 740 1858 1878
Size: 451 137 152 1118 20
Fragments arranged by size:
1118 451 152 137 20
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 691 773 1699 1852 1878
Size: 691 82 926 153 26
Fragments arranged by size:
926 691 153 82 26
Bpu10I CC'TnA_GC
Cuts at: 0 621 878 956 1878
Size: 621 257 78 922
Fragments arranged by size:
922 621 257 78
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1768 1878
Size: 1768 110
BsaI GGTCTCn'nnnn_
Cuts at: 0 409 1878
Size: 409 1469
BsaHI Gr'CG_yC
Cuts at: 0 7 275 523 1878
Size: 7 268 248 1355
Fragments arranged by size:
1355 268 248 7
BsaWI w'CCGG_w
Cuts at: 0 1130 1878
Size: 1130 748
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 62 92 1747 1777 1878
Size: 62 30 1655 30 101
Fragments arranged by size:
1655 101 62 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 334 820 1843 1878
Size: 334 486 1023 35
Fragments arranged by size:
1023 486 334 35
BseYI C'CCAG_C
Cuts at: 0 129 370 1278 1878
Size: 129 241 908 600
Fragments arranged by size:
908 600 241 129
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1626 1878
Size: 1626 252
BsiHKAI G_wGCw'C
Cuts at: 0 62 758 1878
Size: 62 696 1120
Fragments arranged by size:
1120 696 62
BsmBI CGTCTCn'nnnn_
Cuts at: 0 24 156 381 1878
Size: 24 132 225 1497
Fragments arranged by size:
1497 225 132 24
Bsp1286I G_dGCh'C
Cuts at: 0 62 100 136 183 239 495 622 758
Size: 62 38 36 47 56 256 127 136
Cuts at: 758 879 1864 1873 1878
Size: 121 985 9 5
Fragments arranged by size:
985 256 136 127 121 62 56 47
38 36 9 5
BspEI T'CCGG_A
Cuts at: 0 1130 1878
Size: 1130 748
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 404 980 1094 1878
Size: 404 576 114 784
Fragments arranged by size:
784 576 404 114
BsrBI CCG'CTC
Cuts at: 0 259 1805 1878
Size: 259 1546 73
Fragments arranged by size:
1546 259 73
BsrFI r'CCGG_y
Cuts at: 0 307 1878
Size: 307 1571
BsrGI T'GTAC_A
Cuts at: 0 641 1878
Size: 641 1237
BstEII G'GTnAC_C
Cuts at: 0 1647 1878
Size: 1647 231
BstXI CCAn_nnnn'nTGG
Cuts at: 0 694 1677 1878
Size: 694 983 201
Fragments arranged by size:
983 694 201
Bsu36I CC'TnA_GG
Cuts at: 0 46 82 420 899 1513 1878
Size: 46 36 338 479 614 365
Fragments arranged by size:
614 479 365 338 46 36
BtgI C'CryG_G
Cuts at: 0 239 532 920 1250 1878
Size: 239 293 388 330 628
Fragments arranged by size:
628 388 330 293 239
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 119 1878
Size: 119 1759
BtsI GCAGTG_nn'
Cuts at: 0 659 932 1878
Size: 659 273 946
Fragments arranged by size:
946 659 273
EaeI y'GGCC_r
Cuts at: 0 777 888 1433 1637 1800 1878
Size: 777 111 545 204 163 78
Fragments arranged by size:
777 545 204 163 111 78
EarI CTCTTCn'nnn_
Cuts at: 0 1202 1878
Size: 1202 676
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 280 1423 1507 1878
Size: 280 1143 84 371
Fragments arranged by size:
1143 371 280 84
EcoICRI GAG'CTC
Cuts at: 0 60 756 1878
Size: 60 696 1122
Fragments arranged by size:
1122 696 60
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 691 773 1318 1699 1852 1878
Size: 691 82 545 381 153 26
Fragments arranged by size:
691 545 381 153 82 26
EcoNI CCTnn'n_nnAGG
Cuts at: 0 559 1357 1878
Size: 559 798 521
Fragments arranged by size:
798 559 521
EcoO109I rG'GnC_Cy
Cuts at: 0 86 179 180 235 262 701 882 1503
Size: 86 93 1 55 27 439 181 621
Cuts at: 1503 1844 1878
Size: 341 34
Fragments arranged by size:
621 439 341 181 93 86 55 34
27 1
EcoRI G'AATT_C
Cuts at: 0 1244 1878
Size: 1244 634
HaeII r_GCGC'y
Cuts at: 0 10 314 552 1878
Size: 10 304 238 1326
Fragments arranged by size:
1326 304 238 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 56 62 88 94 1569 1601 1878
Size: 56 6 26 6 1475 32 277
Fragments arranged by size:
1475 277 56 32 26 6 6
HincII GTy'rAC
Cuts at: 0 1125 1878
Size: 1125 753
HindIII A'AGCT_T
Cuts at: 0 1684 1744 1878
Size: 1684 60 134
Fragments arranged by size:
1684 134 60
KasI G'GCGC_C
Cuts at: 0 6 1878
Size: 6 1872
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 831 1098 1769 1878
Size: 831 267 671 109
Fragments arranged by size:
831 671 267 109
MscI TGG'CCA
Cuts at: 0 890 1639 1878
Size: 890 749 239
Fragments arranged by size:
890 749 239
MslI CAynn'nnrTG
Cuts at: 0 919 1426 1540 1645 1783 1878
Size: 919 507 114 105 138 95
Fragments arranged by size:
919 507 138 114 105 95
MspA1I CmG'CkG
Cuts at: 0 212 218 241 1398 1458 1878
Size: 212 6 23 1157 60 420
Fragments arranged by size:
1157 420 212 60 23 6
NaeI GCC'GGC
Cuts at: 0 309 1878
Size: 309 1569
NarI GG'CG_CC
Cuts at: 0 7 1878
Size: 7 1871
NdeI CA'TA_TG
Cuts at: 0 965 1878
Size: 965 913
NgoMIV G'CCGG_C
Cuts at: 0 307 1878
Size: 307 1571
NruI TCG'CGA
Cuts at: 0 104 1878
Size: 104 1774
NspI r_CATG'y
Cuts at: 0 514 1293 1878
Size: 514 779 585
Fragments arranged by size:
779 585 514
PflMI CCAn_nnn'nTGG
Cuts at: 0 386 1566 1878
Size: 386 1180 312
Fragments arranged by size:
1180 386 312
PfoI T'CCnGG_A
Cuts at: 0 668 1829 1878
Size: 668 1161 49
Fragments arranged by size:
1161 668 49
PpuMI rG'GwC_Cy
Cuts at: 0 262 882 1878
Size: 262 620 996
Fragments arranged by size:
996 620 262
PspOMI G'GGCC_C
Cuts at: 0 179 235 1860 1878
Size: 179 56 1625 18
Fragments arranged by size:
1625 179 56 18
PspXI vC'TCGA_Gb
Cuts at: 0 1380 1878
Size: 1380 498
PstI C_TGCA'G
Cuts at: 0 217 1403 1502 1878
Size: 217 1186 99 376
Fragments arranged by size:
1186 376 217 99
PvuII CAG'CTG
Cuts at: 0 1398 1878
Size: 1398 480
SacI G_AGCT'C
Cuts at: 0 62 758 1878
Size: 62 696 1120
Fragments arranged by size:
1120 696 62
SacII CC_GC'GG
Cuts at: 0 242 1878
Size: 242 1636
SexAI A'CCwGG_T
Cuts at: 0 845 1878
Size: 845 1033
SfcI C'TryA_G
Cuts at: 0 213 1399 1498 1878
Size: 213 1186 99 380
Fragments arranged by size:
1186 380 213 99
SfoI GGC'GCC
Cuts at: 0 8 1878
Size: 8 1870
SgrAI Cr'CCGG_yG
Cuts at: 0 307 1878
Size: 307 1571
SmaI CCC'GGG
Cuts at: 0 1594 1878
Size: 1594 284
SmlI C'TyrA_G
Cuts at: 0 1380 1747 1878
Size: 1380 367 131
Fragments arranged by size:
1380 367 131
SrfI GCCC'GGGC
Cuts at: 0 1594 1878
Size: 1594 284
StyI C'CwwG_G
Cuts at: 0 143 768 1878
Size: 143 625 1110
Fragments arranged by size:
1110 625 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 160 1878
Size: 160 1718
TatI w'GTAC_w
Cuts at: 0 641 1878
Size: 641 1237
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1235 1267 1878
Size: 1235 32 611
Fragments arranged by size:
1235 611 32
Tth111I GACn'n_nGTC
Cuts at: 0 682 1878
Size: 682 1196
XbaI T'CTAG_A
Cuts at: 0 816 1107 1878
Size: 816 291 771
Fragments arranged by size:
816 771 291
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1022 1188 1785 1878
Size: 1022 166 597 93
Fragments arranged by size:
1022 597 166 93
XhoI C'TCGA_G
Cuts at: 0 1380 1878
Size: 1380 498
XmaI C'CCGG_G
Cuts at: 0 1592 1878
Size: 1592 286
XmnI GAAnn'nnTTC
Cuts at: 0 328 1878
Size: 328 1550
Enzymes that do cut:
AarI AcuI AfeI AleI AlwNI ApaI ApoI AvaI
BanI BanII BbeI BbsI BcgI BciVI BlpI Bme1580I
BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI
BspMI BsrBI BsrFI BsrGI BstEII BstXI Bsu36I BtgI
BtgZI BtsI EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI HaeII Hin4I HincII HindIII KasI MmeI
MscI MslI MspA1I NaeI NarI NdeI NgoMIV NruI
NspI PflMI PfoI PpuMI PspOMI PspXI PstI PvuII
SacI SacII SexAI SfcI SfoI SgrAI SmaI SmlI
SrfI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbvCI BclI BfrBI BglI BglII BmtI BplI
BsaAI BsaBI BsiEI BsiWI BsmI BspHI BsrDI BssHII
BssSI BstAPI BstBI BstYI BstZ17I ClaI CspCI DraI
DraIII DrdI EagI EcoRV FalI FseI FspI FspAI
HpaI KpnI MfeI MluI NcoI NheI NotI NsiI
PacI PasI PciI PmeI PmlI PpiI PshAI PsiI
PsrI PvuI RsrII SalI SanDI SapI SbfI ScaI
SfiI SgfI SnaBI SpeI SphI SspI StuI SwaI
ZraI