Restriction map for
pFN21AE3259
(Linear) (Six-base) MAPSORT of: KIEE3259.seq Check: 9461 from: 1 to: 1851
KIEE3259.orf
With 182 enzymes: SgfI *
July 3, 2012 17:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 781 1091 1851
Size: 781 310 760
Fragments arranged by size:
781 760 310
Acc65I G'GTAC_C
Cuts at: 0 1483 1851
Size: 1483 368
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1115 1301 1851
Size: 1115 186 550
Fragments arranged by size:
1115 550 186
AflIII A'CryG_T
Cuts at: 0 76 764 1851
Size: 76 688 1087
Fragments arranged by size:
1087 688 76
AleI CACnn'nnGTG
Cuts at: 0 343 1851
Size: 343 1508
AlwNI CAG_nnn'CTG
Cuts at: 0 1846 1851
Size: 1846 5
ApaI G_GGCC'C
Cuts at: 0 1006 1851
Size: 1006 845
ApaLI G'TGCA_C
Cuts at: 0 64 768 1851
Size: 64 704 1083
Fragments arranged by size:
1083 704 64
ApoI r'AATT_y
Cuts at: 0 104 868 1684 1773 1851
Size: 104 764 816 89 78
Fragments arranged by size:
816 764 104 89 78
BanI G'GyrC_C
Cuts at: 0 1188 1288 1339 1483 1497 1561 1851
Size: 1188 100 51 144 14 64 290
Fragments arranged by size:
1188 290 144 100 64 51 14
BanII G_rGCy'C
Cuts at: 0 648 1006 1064 1851
Size: 648 358 58 787
Fragments arranged by size:
787 648 358 58
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1342 1376 1851
Size: 1342 34 475
Fragments arranged by size:
1342 475 34
BglI GCCn_nnn'nGGC
Cuts at: 0 785 1851
Size: 785 1066
BglII A'GATC_T
Cuts at: 0 1442 1851
Size: 1442 409
Bme1580I G_kGCm'C
Cuts at: 0 68 350 772 1006 1291 1637 1851
Size: 68 282 422 234 285 346 214
Fragments arranged by size:
422 346 285 282 234 214 68
BmgBI CAC'GTC
Cuts at: 0 681 1851
Size: 681 1170
BmrI ACTGGGnnnn_n'
Cuts at: 0 344 1851
Size: 344 1507
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 683 946 1851
Size: 683 263 905
Fragments arranged by size:
905 683 263
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1661 1851
Size: 1661 190
BsaAI yAC'GTr
Cuts at: 0 767 1851
Size: 767 1084
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 129 141 1132 1135 1147 1222 1225 1228
Size: 129 12 991 3 12 75 3 3
Cuts at: 1228 1249 1252 1255 1599 1851
Size: 21 3 3 344 252
Fragments arranged by size:
991 344 252 129 75 21 12 12
3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 1575 1851
Size: 1575 276
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 813 1063 1851
Size: 813 250 788
Fragments arranged by size:
813 788 250
BsiHKAI G_wGCw'C
Cuts at: 0 68 648 772 987 1851
Size: 68 580 124 215 864
Fragments arranged by size:
864 580 215 124 68
BsmI GAATG_Cn'
Cuts at: 0 1374 1851
Size: 1374 477
Bsp1286I G_dGCh'C
Cuts at: 0 68 350 648 772 987 1006 1064 1291
Size: 68 282 298 124 215 19 58 227
Cuts at: 1291 1637 1851
Size: 346 214
Fragments arranged by size:
346 298 282 227 215 214 124 68
58 19
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 61 781 847 1091 1851
Size: 61 720 66 244 760
Fragments arranged by size:
760 720 244 66 61
BsrGI T'GTAC_A
Cuts at: 0 288 1851
Size: 288 1563
BssSI C'ACGA_G
Cuts at: 0 719 1851
Size: 719 1132
BstAPI GCAn_nnn'nTGC
Cuts at: 0 794 1151 1369 1851
Size: 794 357 218 482
Fragments arranged by size:
794 482 357 218
BstYI r'GATC_y
Cuts at: 0 1442 1851
Size: 1442 409
Bsu36I CC'TnA_GG
Cuts at: 0 469 1851
Size: 469 1382
BtgI C'CryG_G
Cuts at: 0 1829 1851
Size: 1829 22
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 109 1851
Size: 109 1742
BtsI GCAGTG_nn'
Cuts at: 0 86 192 1079 1851
Size: 86 106 887 772
Fragments arranged by size:
887 772 106 86
DraI TTT'AAA
Cuts at: 0 110 1851
Size: 110 1741
DraIII CAC_nnn'GTG
Cuts at: 0 1195 1851
Size: 1195 656
EarI CTCTTCn'nnn_
Cuts at: 0 636 1124 1220 1226 1316 1395 1544 1617
Size: 636 488 96 6 90 79 149 73
Cuts at: 1617 1805 1851
Size: 188 46
Fragments arranged by size:
636 488 188 149 96 90 79 73
46 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 700 1851
Size: 700 1151
EcoICRI GAG'CTC
Cuts at: 0 646 1851
Size: 646 1205
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 683 946 1115 1301 1851
Size: 683 263 169 186 550
Fragments arranged by size:
683 550 263 186 169
EcoNI CCTnn'n_nnAGG
Cuts at: 0 156 472 1851
Size: 156 316 1379
Fragments arranged by size:
1379 316 156
EcoO109I rG'GnC_Cy
Cuts at: 0 1003 1705 1851
Size: 1003 702 146
Fragments arranged by size:
1003 702 146
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 124 156 796 828 969 1001 1457 1489
Size: 124 32 640 32 141 32 456 32
Cuts at: 1489 1851
Size: 362
Fragments arranged by size:
640 456 362 141 124 32 32 32
32
HincII GTy'rAC
Cuts at: 0 132 1530 1851
Size: 132 1398 321
Fragments arranged by size:
1398 321 132
HindIII A'AGCT_T
Cuts at: 0 1182 1851
Size: 1182 669
KpnI G_GTAC'C
Cuts at: 0 1487 1851
Size: 1487 364
MslI CAynn'nnrTG
Cuts at: 0 343 910 1851
Size: 343 567 941
Fragments arranged by size:
941 567 343
MspA1I CmG'CkG
Cuts at: 0 1157 1579 1851
Size: 1157 422 272
Fragments arranged by size:
1157 422 272
NspI r_CATG'y
Cuts at: 0 80 1851
Size: 80 1771
PasI CC'CwG_GG
Cuts at: 0 369 1851
Size: 369 1482
PciI A'CATG_T
Cuts at: 0 76 1851
Size: 76 1775
PfoI T'CCnGG_A
Cuts at: 0 1425 1851
Size: 1425 426
PmlI CAC'GTG
Cuts at: 0 767 1851
Size: 767 1084
PspOMI G'GGCC_C
Cuts at: 0 1002 1851
Size: 1002 849
PstI C_TGCA'G
Cuts at: 0 93 778 1074 1088 1851
Size: 93 685 296 14 763
Fragments arranged by size:
763 685 296 93 14
PvuII CAG'CTG
Cuts at: 0 1579 1851
Size: 1579 272
SacI G_AGCT'C
Cuts at: 0 648 1851
Size: 648 1203
SapI GCTCTTCn'nnn_
Cuts at: 0 636 1851
Size: 636 1215
ScaI AGT'ACT
Cuts at: 0 607 1851
Size: 607 1244
SfcI C'TryA_G
Cuts at: 0 89 774 1070 1084 1851
Size: 89 685 296 14 767
Fragments arranged by size:
767 685 296 89 14
SmlI C'TyrA_G
Cuts at: 0 1676 1851
Size: 1676 175
SspI AAT'ATT
Cuts at: 0 1027 1851
Size: 1027 824
StuI AGG'CCT
Cuts at: 0 1555 1851
Size: 1555 296
StyI C'CwwG_G
Cuts at: 0 1110 1851
Size: 1110 741
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1306 1851
Size: 1306 545
TatI w'GTAC_w
Cuts at: 0 288 311 404 605 1851
Size: 288 23 93 201 1246
Fragments arranged by size:
1246 288 201 93 23
Tth111I GACn'n_nGTC
Cuts at: 0 211 1471 1851
Size: 211 1260 380
Fragments arranged by size:
1260 380 211
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 409 1851
Size: 409 1442
Enzymes that do cut:
AarI Acc65I AcuI AflIII AleI AlwNI ApaI ApaLI
ApoI BanI BanII BcgI BglI BglII Bme1580I BmgBI
BmrI BpmI BpuEI BsaAI BseRI BseYI BsgI BsiHKAI
BsmI Bsp1286I BspMI BsrGI BssSI BstAPI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I Hin4I HincII HindIII KpnI MslI
MspA1I NspI PasI PciI PfoI PmlI PspOMI PstI
PvuII SacI SapI ScaI SfcI SmlI SspI StuI
StyI TaqII TatI Tth111I XcmI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AgeI AhdI AlfI
AloI AscI AseI AsiSI AvaI AvrII BaeI BamHI
BbeI BbsI BbvCI BciVI BclI BfrBI BlpI BmtI
BplI Bpu10I BsaI BsaBI BsaHI BsaWI BsaXI BsiEI
BsiWI BsmBI BspEI BspHI BsrBI BsrDI BsrFI BssHII
BstBI BstEII BstXI BstZ17I ClaI CspCI DrdI EaeI
EagI EcoRI EcoRV FalI FseI FspI FspAI HaeII
HpaI KasI MfeI MluI MmeI MscI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PsrI
PvuI RsrII SacII SalI SanDI SbfI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SwaI TstI XbaI XhoI XmaI XmnI ZraI