Restriction map for
pFN21AE3252
(Linear) (Six-base) MAPSORT of: KIEE3252.seq Check: 1932 from: 1 to: 1764
KIEE3252.orf
With 182 enzymes: SgfI *
July 3, 2012 17:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 355 1764
Size: 355 1409
AccI GT'mk_AC
Cuts at: 0 407 1764
Size: 407 1357
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 599 731 1429 1764
Size: 599 132 698 335
Fragments arranged by size:
698 599 335 132
AflII C'TTAA_G
Cuts at: 0 660 1764
Size: 660 1104
AhdI GACnn_n'nnGTC
Cuts at: 0 1139 1764
Size: 1139 625
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 148 1764
Size: 148 1616
AlwNI CAG_nnn'CTG
Cuts at: 0 205 1407 1764
Size: 205 1202 357
Fragments arranged by size:
1202 357 205
ApaI G_GGCC'C
Cuts at: 0 1612 1764
Size: 1612 152
ApaLI G'TGCA_C
Cuts at: 0 1253 1764
Size: 1253 511
ApoI r'AATT_y
Cuts at: 0 179 395 1484 1764
Size: 179 216 1089 280
Fragments arranged by size:
1089 280 216 179
AseI AT'TA_AT
Cuts at: 0 332 1764
Size: 332 1432
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 370 403 1764
Size: 370 33 1361
Fragments arranged by size:
1361 370 33
BamHI G'GATC_C
Cuts at: 0 1749 1764
Size: 1749 15
BanII G_rGCy'C
Cuts at: 0 1612 1662 1764
Size: 1612 50 102
Fragments arranged by size:
1612 102 50
BbsI GAAGACnn'nnnn_
Cuts at: 0 179 1028 1109 1764
Size: 179 849 81 655
Fragments arranged by size:
849 655 179 81
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 264 298 1764
Size: 264 34 1466
Fragments arranged by size:
1466 264 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 398 1764
Size: 398 1366
BlpI GC'TnA_GC
Cuts at: 0 851 1764
Size: 851 913
Bme1580I G_kGCm'C
Cuts at: 0 1257 1612 1764
Size: 1257 355 152
Fragments arranged by size:
1257 355 152
BmrI ACTGGGnnnn_n'
Cuts at: 0 1210 1656 1764
Size: 1210 446 108
Fragments arranged by size:
1210 446 108
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 657 1764
Size: 657 1107
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1057 1764
Size: 1057 707
Bpu10I CC'TnA_GC
Cuts at: 0 1469 1764
Size: 1469 295
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 668 1764
Size: 668 1096
BsaWI w'CCGG_w
Cuts at: 0 483 1764
Size: 483 1281
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 361 1764
Size: 361 1403
BsiHKAI G_wGCw'C
Cuts at: 0 575 1257 1764
Size: 575 682 507
Fragments arranged by size:
682 575 507
BsmI GAATG_Cn'
Cuts at: 0 1584 1764
Size: 1584 180
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1590 1764
Size: 1590 174
Bsp1286I G_dGCh'C
Cuts at: 0 575 1257 1612 1662 1764
Size: 575 682 355 50 102
Fragments arranged by size:
682 575 355 102 50
BspHI T'CATG_A
Cuts at: 0 981 1559 1764
Size: 981 578 205
Fragments arranged by size:
981 578 205
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 355 1764
Size: 355 1409
BsrDI GCAATG_nn'
Cuts at: 0 137 1764
Size: 137 1627
BsrFI r'CCGG_y
Cuts at: 0 1278 1764
Size: 1278 486
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1261 1764
Size: 1261 503
BstBI TT'CG_AA
Cuts at: 0 629 1764
Size: 629 1135
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1294 1764
Size: 1294 470
BstYI r'GATC_y
Cuts at: 0 1403 1749 1764
Size: 1403 346 15
Fragments arranged by size:
1403 346 15
Bsu36I CC'TnA_GG
Cuts at: 0 767 1754 1764
Size: 767 987 10
Fragments arranged by size:
987 767 10
BtgI C'CryG_G
Cuts at: 0 1695 1764
Size: 1695 69
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1279 1764
Size: 1279 485
ClaI AT'CG_AT
Cuts at: 0 341 1764
Size: 341 1423
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 37 72 1764
Size: 37 35 1692
Fragments arranged by size:
1692 37 35
DraI TTT'AAA
Cuts at: 0 921 1764
Size: 921 843
DraIII CAC_nnn'GTG
Cuts at: 0 442 1764
Size: 442 1322
EarI CTCTTCn'nnn_
Cuts at: 0 626 863 1352 1764
Size: 626 237 489 412
Fragments arranged by size:
626 489 412 237
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 599 731 1057 1429 1764
Size: 599 132 326 372 335
Fragments arranged by size:
599 372 335 326 132
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 959 991 1125 1157 1359 1388 1391 1420
Size: 959 32 134 32 202 29 3 29
Cuts at: 1420 1764
Size: 344
Fragments arranged by size:
959 344 202 134 32 32 29 29
3
HincII GTy'rAC
Cuts at: 0 594 1284 1764
Size: 594 690 480
Fragments arranged by size:
690 594 480
MspA1I CmG'CkG
Cuts at: 0 1318 1764
Size: 1318 446
NcoI C'CATG_G
Cuts at: 0 1695 1764
Size: 1695 69
PsiI TTA'TAA
Cuts at: 0 1517 1764
Size: 1517 247
PspOMI G'GGCC_C
Cuts at: 0 1608 1764
Size: 1608 156
SfcI C'TryA_G
Cuts at: 0 600 1764
Size: 600 1164
SmlI C'TyrA_G
Cuts at: 0 660 683 1764
Size: 660 23 1081
Fragments arranged by size:
1081 660 23
SspI AAT'ATT
Cuts at: 0 1234 1764
Size: 1234 530
StyI C'CwwG_G
Cuts at: 0 1695 1764
Size: 1695 69
XbaI T'CTAG_A
Cuts at: 0 510 1764
Size: 510 1254
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 112 787 1764
Size: 112 675 977
Fragments arranged by size:
977 675 112
XmnI GAAnn'nnTTC
Cuts at: 0 290 449 1235 1456 1764
Size: 290 159 786 221 308
Fragments arranged by size:
786 308 290 221 159
Enzymes that do cut:
AarI AccI AcuI AflII AhdI AlfI AlwNI ApaI
ApaLI ApoI AseI BaeI BamHI BanII BbsI BcgI
BciVI BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaWI BseRI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI
BsrDI BsrFI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI ClaI CspCI DraI DraIII EarI Eco57MI Hin4I
HincII MspA1I NcoI PsiI PspOMI SfcI SmlI SspI
StyI XbaI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflIII AgeI AleI AloI
AscI AsiSI AvaI AvrII BanI BbeI BbvCI BclI
BfrBI BglI BglII BmgBI BmtI BsaI BsaAI BsaBI
BsaHI BsaXI BseYI BsgI BsiEI BsiWI BspEI BsrBI
BsrGI BssHII BssSI BstEII BstZ17I BtsI DrdI EaeI
EagI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI
FseI FspI FspAI HaeII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PspXI PsrI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI
SwaI TaqII TatI TstI Tth111I XhoI XmaI ZraI