Restriction map for
pFN21AE3248
(Linear) (Six-base) MAPSORT of: KIEE3248.seq Check: 8762 from: 1 to: 1713
KIEE3248.orf
With 182 enzymes: SgfI *
December 27, 2011 16:34 ..
AccI GT'mk_AC
Cuts at: 0 124 1713
Size: 124 1589
Acc65I G'GTAC_C
Cuts at: 0 188 923 1713
Size: 188 735 790
Fragments arranged by size:
790 735 188
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 743 1342 1713
Size: 743 599 371
Fragments arranged by size:
743 599 371
AflII C'TTAA_G
Cuts at: 0 981 1713
Size: 981 732
ApoI r'AATT_y
Cuts at: 0 204 597 1611 1713
Size: 204 393 1014 102
Fragments arranged by size:
1014 393 204 102
BamHI G'GATC_C
Cuts at: 0 1197 1405 1713
Size: 1197 208 308
Fragments arranged by size:
1197 308 208
BanI G'GyrC_C
Cuts at: 0 188 913 923 1386 1713
Size: 188 725 10 463 327
Fragments arranged by size:
725 463 327 188 10
BanII G_rGCy'C
Cuts at: 0 77 1713
Size: 77 1636
BciVI GTATCCnnnnn_n'
Cuts at: 0 1647 1713
Size: 1647 66
BclI T'GATC_A
Cuts at: 0 1139 1713
Size: 1139 574
BglI GCCn_nnn'nGGC
Cuts at: 0 1395 1713
Size: 1395 318
BlpI GC'TnA_GC
Cuts at: 0 544 1713
Size: 544 1169
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1554 1713
Size: 1554 159
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 686 1430 1713
Size: 686 744 283
Fragments arranged by size:
744 686 283
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1524 1713
Size: 1524 189
BsaBI GATnn'nnATC
Cuts at: 0 308 1418 1713
Size: 308 1110 295
Fragments arranged by size:
1110 308 295
BsaHI Gr'CG_yC
Cuts at: 0 17 1713
Size: 17 1696
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 816 846 1713
Size: 816 30 867
Fragments arranged by size:
867 816 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 469 1713
Size: 469 1244
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 887 1713
Size: 887 826
BsiEI CG_ry'CG
Cuts at: 0 128 1713
Size: 128 1585
BsiHKAI G_wGCw'C
Cuts at: 0 1650 1713
Size: 1650 63
Bsp1286I G_dGCh'C
Cuts at: 0 77 1650 1713
Size: 77 1573 63
Fragments arranged by size:
1573 77 63
BsrFI r'CCGG_y
Cuts at: 0 1394 1713
Size: 1394 319
BsrGI T'GTAC_A
Cuts at: 0 729 1713
Size: 729 984
BssSI C'ACGA_G
Cuts at: 0 67 988 1713
Size: 67 921 725
Fragments arranged by size:
921 725 67
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1386 1713
Size: 1386 327
BstYI r'GATC_y
Cuts at: 0 50 1197 1405 1713
Size: 50 1147 208 308
Fragments arranged by size:
1147 308 208 50
BtgI C'CryG_G
Cuts at: 0 895 1713
Size: 895 818
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 514 1713
Size: 514 1199
DraI TTT'AAA
Cuts at: 0 1568 1713
Size: 1568 145
DraIII CAC_nnn'GTG
Cuts at: 0 104 1713
Size: 104 1609
EaeI y'GGCC_r
Cuts at: 0 279 1713
Size: 279 1434
EarI CTCTTCn'nnn_
Cuts at: 0 345 356 458 489 1118 1713
Size: 345 11 102 31 629 595
Fragments arranged by size:
629 595 345 102 31 11
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 686 743 1342 1430 1713
Size: 686 57 599 88 283
Fragments arranged by size:
686 599 283 88 57
EcoRI G'AATT_C
Cuts at: 0 597 1713
Size: 597 1116
EcoRV GAT'ATC
Cuts at: 0 842 1713
Size: 842 871
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 739 771 816 848 1461 1493 1713
Size: 739 32 45 32 613 32 220
Fragments arranged by size:
739 613 220 45 32 32 32
HincII GTy'rAC
Cuts at: 0 125 216 1713
Size: 125 91 1497
Fragments arranged by size:
1497 125 91
HindIII A'AGCT_T
Cuts at: 0 265 1713
Size: 265 1448
KpnI G_GTAC'C
Cuts at: 0 192 927 1713
Size: 192 735 786
Fragments arranged by size:
786 735 192
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 834 1546 1713
Size: 834 712 167
Fragments arranged by size:
834 712 167
MscI TGG'CCA
Cuts at: 0 281 1713
Size: 281 1432
MslI CAynn'nnrTG
Cuts at: 0 900 1713
Size: 900 813
NcoI C'CATG_G
Cuts at: 0 895 1713
Size: 895 818
PasI CC'CwG_GG
Cuts at: 0 254 1713
Size: 254 1459
PstI C_TGCA'G
Cuts at: 0 1032 1713
Size: 1032 681
SalI G'TCGA_C
Cuts at: 0 123 1713
Size: 123 1590
SapI GCTCTTCn'nnn_
Cuts at: 0 489 1713
Size: 489 1224
SfcI C'TryA_G
Cuts at: 0 1028 1657 1713
Size: 1028 629 56
Fragments arranged by size:
1028 629 56
SmlI C'TyrA_G
Cuts at: 0 981 1539 1713
Size: 981 558 174
Fragments arranged by size:
981 558 174
StyI C'CwwG_G
Cuts at: 0 846 895 1713
Size: 846 49 818
Fragments arranged by size:
846 818 49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 639 1049 1713
Size: 639 410 664
Fragments arranged by size:
664 639 410
TatI w'GTAC_w
Cuts at: 0 729 1713
Size: 729 984
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1503 1535 1713
Size: 1503 32 178
Fragments arranged by size:
1503 178 32
XmnI GAAnn'nnTTC
Cuts at: 0 204 1713
Size: 204 1509
Enzymes that do cut:
AccI Acc65I AcuI AflII ApoI BamHI BanI BanII
BciVI BclI BglI BlpI BplI BpmI BpuEI BsaBI
BsaHI BsaXI BseRI BsgI BsiEI BsiHKAI Bsp1286I BsrFI
BsrGI BssSI BstAPI BstYI BtgI BtgZI DraI DraIII
EaeI EarI Eco57MI EcoRI EcoRV Hin4I HincII HindIII
KpnI MmeI MscI MslI NcoI PasI PstI SalI
SapI SfcI SmlI StyI TaqII TatI TstI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflIII AgeI AhdI AleI
AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BbeI BbsI BbvCI BcgI BfrBI
BglII Bme1580I BmgBI BmrI BmtI Bpu10I BsaI BsaAI
BsaWI BseYI BsiWI BsmI BsmBI BspEI BspHI BspMI
BsrBI BsrDI BssHII BstBI BstEII BstXI BstZ17I Bsu36I
BtsI ClaI CspCI DrdI EagI EciI EcoICRI EcoNI
EcoO109I FalI FseI FspI FspAI HaeII HpaI KasI
MfeI MluI MspA1I NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI PvuII RsrII SacI SacII SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI
XcmI XhoI XmaI ZraI