Restriction map for
pFN21AE3247
(Linear) (Six-base) MAPSORT of: KIEE3247.seq Check: 991 from: 1 to: 1710
KIEE3247.orf
With 182 enzymes: SgfI *
December 27, 2011 16:34 ..
AatII G_ACGT'C
Cuts at: 0 272 1710
Size: 272 1438
AccI GT'mk_AC
Cuts at: 0 1227 1710
Size: 1227 483
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1033 1041 1048 1442 1556 1652 1710
Size: 1033 8 7 394 114 96 58
Fragments arranged by size:
1033 394 114 96 58 8 7
AgeI A'CCGG_T
Cuts at: 0 523 1710
Size: 523 1187
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 160 1710
Size: 160 1550
AlwNI CAG_nnn'CTG
Cuts at: 0 1699 1710
Size: 1699 11
ApaLI G'TGCA_C
Cuts at: 0 1165 1710
Size: 1165 545
ApoI r'AATT_y
Cuts at: 0 1312 1710
Size: 1312 398
AvaI C'yCGr_G
Cuts at: 0 274 1133 1710
Size: 274 859 577
Fragments arranged by size:
859 577 274
BanI G'GyrC_C
Cuts at: 0 117 1399 1710
Size: 117 1282 311
Fragments arranged by size:
1282 311 117
BanII G_rGCy'C
Cuts at: 0 86 1493 1710
Size: 86 1407 217
Fragments arranged by size:
1407 217 86
BbeI G_GCGC'C
Cuts at: 0 121 1403 1710
Size: 121 1282 307
Fragments arranged by size:
1282 307 121
BbsI GAAGACnn'nnnn_
Cuts at: 0 401 873 1465 1710
Size: 401 472 592 245
Fragments arranged by size:
592 472 401 245
BbvCI CC'TCA_GC
Cuts at: 0 148 1710
Size: 148 1562
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 323 357 1710
Size: 323 34 1353
Fragments arranged by size:
1353 323 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 236 1710
Size: 236 1474
BfrBI ATG'CAT
Cuts at: 0 344 1710
Size: 344 1366
BglI GCCn_nnn'nGGC
Cuts at: 0 70 181 1710
Size: 70 111 1529
Fragments arranged by size:
1529 111 70
BglII A'GATC_T
Cuts at: 0 1208 1598 1710
Size: 1208 390 112
Fragments arranged by size:
1208 390 112
Bme1580I G_kGCm'C
Cuts at: 0 1169 1432 1710
Size: 1169 263 278
Fragments arranged by size:
1169 278 263
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 293 587 1532 1710
Size: 293 294 945 178
Fragments arranged by size:
945 294 293 178
Bpu10I CC'TnA_GC
Cuts at: 0 148 1487 1710
Size: 148 1339 223
Fragments arranged by size:
1339 223 148
BsaI GGTCTCn'nnnn_
Cuts at: 0 18 691 1710
Size: 18 673 1019
Fragments arranged by size:
1019 673 18
BsaBI GATnn'nnATC
Cuts at: 0 368 652 1710
Size: 368 284 1058
Fragments arranged by size:
1058 368 284
BsaHI Gr'CG_yC
Cuts at: 0 118 269 1400 1710
Size: 118 151 1131 310
Fragments arranged by size:
1131 310 151 118
BsaWI w'CCGG_w
Cuts at: 0 75 523 1169 1710
Size: 75 448 646 541
Fragments arranged by size:
646 541 448 75
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 94 255 646 649 679 832 1710
Size: 94 161 391 3 30 153 878
Fragments arranged by size:
878 391 161 153 94 30 3
BseYI C'CCAG_C
Cuts at: 0 250 1710
Size: 250 1460
BsiEI CG_ry'CG
Cuts at: 0 110 185 1710
Size: 110 75 1525
Fragments arranged by size:
1525 110 75
BsiHKAI G_wGCw'C
Cuts at: 0 1134 1169 1710
Size: 1134 35 541
Fragments arranged by size:
1134 541 35
BsmBI CGTCTCn'nnnn_
Cuts at: 0 4 311 1710
Size: 4 307 1399
Fragments arranged by size:
1399 307 4
Bsp1286I G_dGCh'C
Cuts at: 0 86 1134 1169 1432 1493 1710
Size: 86 1048 35 263 61 217
Fragments arranged by size:
1048 263 217 86 61 35
BspEI T'CCGG_A
Cuts at: 0 75 1710
Size: 75 1635
BsrDI GCAATG_nn'
Cuts at: 0 425 529 1710
Size: 425 104 1181
Fragments arranged by size:
1181 425 104
BsrFI r'CCGG_y
Cuts at: 0 233 523 1710
Size: 233 290 1187
Fragments arranged by size:
1187 290 233
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1077 1710
Size: 1077 633
BstYI r'GATC_y
Cuts at: 0 369 1208 1598 1710
Size: 369 839 390 112
Fragments arranged by size:
839 390 369 112
Bsu36I CC'TnA_GG
Cuts at: 0 101 413 1361 1710
Size: 101 312 948 349
Fragments arranged by size:
948 349 312 101
BtgI C'CryG_G
Cuts at: 0 736 1710
Size: 736 974
BtsI GCAGTG_nn'
Cuts at: 0 1169 1566 1626 1710
Size: 1169 397 60 84
Fragments arranged by size:
1169 397 84 60
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1284 1319 1710
Size: 1284 35 391
Fragments arranged by size:
1284 391 35
EaeI y'GGCC_r
Cuts at: 0 62 182 1710
Size: 62 120 1528
Fragments arranged by size:
1528 120 62
EagI C'GGCC_G
Cuts at: 0 182 1710
Size: 182 1528
EarI CTCTTCn'nnn_
Cuts at: 0 11 319 584 617 623 638 656 758
Size: 11 308 265 33 6 15 18 102
Cuts at: 758 809 850 1060 1484 1710
Size: 51 41 210 424 226
Fragments arranged by size:
424 308 265 226 210 102 51 41
33 18 15 11 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 252 1710
Size: 252 1458
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 293 587 1033 1041 1048 1442 1532 1556
Size: 293 294 446 8 7 394 90 24
Cuts at: 1556 1652 1710
Size: 96 58
Fragments arranged by size:
446 394 294 293 96 90 58 24
8 7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 412 1710
Size: 412 1298
EcoRV GAT'ATC
Cuts at: 0 1422 1710
Size: 1422 288
HaeII r_GCGC'y
Cuts at: 0 121 257 1403 1710
Size: 121 136 1146 307
Fragments arranged by size:
1146 307 136 121
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 141 173 583 615 1258 1290 1681 1710
Size: 141 32 410 32 643 32 391 29
Fragments arranged by size:
643 410 391 141 32 32 32 29
HincII GTy'rAC
Cuts at: 0 1228 1710
Size: 1228 482
KasI G'GCGC_C
Cuts at: 0 117 1399 1710
Size: 117 1282 311
Fragments arranged by size:
1282 311 117
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 564 1710
Size: 564 1146
MslI CAynn'nnrTG
Cuts at: 0 1200 1710
Size: 1200 510
MspA1I CmG'CkG
Cuts at: 0 178 199 1710
Size: 178 21 1511
Fragments arranged by size:
1511 178 21
NaeI GCC'GGC
Cuts at: 0 235 1710
Size: 235 1475
NarI GG'CG_CC
Cuts at: 0 118 1400 1710
Size: 118 1282 310
Fragments arranged by size:
1282 310 118
NgoMIV G'CCGG_C
Cuts at: 0 233 1710
Size: 233 1477
NotI GC'GGCC_GC
Cuts at: 0 182 1710
Size: 182 1528
NsiI A_TGCA'T
Cuts at: 0 346 1710
Size: 346 1364
NspI r_CATG'y
Cuts at: 0 1087 1710
Size: 1087 623
PflMI CCAn_nnn'nTGG
Cuts at: 0 1158 1710
Size: 1158 552
PshAI GACnn'nnGTC
Cuts at: 0 884 1710
Size: 884 826
PstI C_TGCA'G
Cuts at: 0 126 1561 1710
Size: 126 1435 149
Fragments arranged by size:
1435 149 126
PvuII CAG'CTG
Cuts at: 0 199 1710
Size: 199 1511
SalI G'TCGA_C
Cuts at: 0 1226 1710
Size: 1226 484
SapI GCTCTTCn'nnn_
Cuts at: 0 11 584 1710
Size: 11 573 1126
Fragments arranged by size:
1126 573 11
SbfI CC_TGCA'GG
Cuts at: 0 126 1710
Size: 126 1584
SfcI C'TryA_G
Cuts at: 0 122 1557 1710
Size: 122 1435 153
Fragments arranged by size:
1435 153 122
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 181 1710
Size: 181 1529
SfoI GGC'GCC
Cuts at: 0 119 1401 1710
Size: 119 1282 309
Fragments arranged by size:
1282 309 119
SgrAI Cr'CCGG_yG
Cuts at: 0 233 1710
Size: 233 1477
SmaI CCC'GGG
Cuts at: 0 276 1710
Size: 276 1434
SspI AAT'ATT
Cuts at: 0 1126 1710
Size: 1126 584
StuI AGG'CCT
Cuts at: 0 261 1710
Size: 261 1449
StyI C'CwwG_G
Cuts at: 0 65 374 850 1493 1710
Size: 65 309 476 643 217
Fragments arranged by size:
643 476 309 217 65
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 179 503 1710
Size: 179 324 1207
Fragments arranged by size:
1207 324 179
XbaI T'CTAG_A
Cuts at: 0 436 1710
Size: 436 1274
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1042 1710
Size: 1042 668
XmaI C'CCGG_G
Cuts at: 0 274 1710
Size: 274 1436
ZraI GAC'GTC
Cuts at: 0 270 1710
Size: 270 1440
Enzymes that do cut:
AatII AccI AcuI AgeI AlfI AlwNI ApaLI ApoI
AvaI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BfrBI BglI BglII Bme1580I BpmI Bpu10I BsaI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BsrDI BsrFI BstAPI BstYI Bsu36I BtgI BtsI
CspCI EaeI EagI EarI EciI Eco57MI EcoNI EcoRV
HaeII Hin4I HincII KasI MmeI MslI MspA1I NaeI
NarI NgoMIV NotI NsiI NspI PflMI PshAI PstI
PvuII SalI SapI SbfI SfcI SfiI SfoI SgrAI
SmaI SspI StuI StyI TaqII XbaI XcmI XmaI
ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AflIII AhdI AleI
AloI ApaI AscI AseI AsiSI AvrII BaeI BamHI
BclI BlpI BmgBI BmrI BmtI BplI BpuEI BsaAI
BsaXI BsgI BsiWI BsmI BspHI BspMI BsrBI BsrGI
BssHII BssSI BstBI BstEII BstXI BstZ17I BtgZI ClaI
DraI DraIII DrdI EcoICRI EcoO109I EcoRI FalI FseI
FspI FspAI HindIII HpaI KpnI MfeI MluI MscI
NcoI NdeI NheI NruI PacI PasI PciI PfoI
PmeI PmlI PpiI PpuMI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SanDI ScaI SexAI SgfI
SmlI SnaBI SpeI SphI SrfI SwaI TatI TstI
Tth111I XhoI XmnI