Restriction map for
pFN21AE3245
(Linear) (Six-base) MAPSORT of: KIEE3245.seq Check: 4273 from: 1 to: 1704
KIEE3245.orf
With 182 enzymes: SgfI *
December 27, 2011 16:34 ..
AccI GT'mk_AC
Cuts at: 0 316 581 1704
Size: 316 265 1123
Fragments arranged by size:
1123 316 265
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1553 1704
Size: 1553 151
AflIII A'CryG_T
Cuts at: 0 1022 1656 1704
Size: 1022 634 48
Fragments arranged by size:
1022 634 48
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1133 1704
Size: 1133 571
AlwNI CAG_nnn'CTG
Cuts at: 0 1531 1704
Size: 1531 173
ApoI r'AATT_y
Cuts at: 0 343 623 679 979 1147 1646 1704
Size: 343 280 56 300 168 499 58
Fragments arranged by size:
499 343 300 280 168 58 56
AseI AT'TA_AT
Cuts at: 0 359 1704
Size: 359 1345
AvaI C'yCGr_G
Cuts at: 0 37 899 1704
Size: 37 862 805
Fragments arranged by size:
862 805 37
AvrII C'CTAG_G
Cuts at: 0 715 1704
Size: 715 989
BamHI G'GATC_C
Cuts at: 0 486 1704
Size: 486 1218
BanII G_rGCy'C
Cuts at: 0 1142 1704
Size: 1142 562
BbsI GAAGACnn'nnnn_
Cuts at: 0 94 128 1684 1704
Size: 94 34 1556 20
Fragments arranged by size:
1556 94 34 20
BglII A'GATC_T
Cuts at: 0 969 1704
Size: 969 735
BmgBI CAC'GTC
Cuts at: 0 1023 1704
Size: 1023 681
BmrI ACTGGGnnnn_n'
Cuts at: 0 216 222 1704
Size: 216 6 1482
Fragments arranged by size:
1482 216 6
BmtI G_CTAG'C
Cuts at: 0 593 1704
Size: 593 1111
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 305 1704
Size: 305 1399
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1089 1704
Size: 1089 615
BsaI GGTCTCn'nnnn_
Cuts at: 0 1538 1704
Size: 1538 166
BsaBI GATnn'nnATC
Cuts at: 0 437 1190 1704
Size: 437 753 514
Fragments arranged by size:
753 514 437
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 43 73 1704
Size: 43 30 1631
Fragments arranged by size:
1631 43 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 51 187 190 193 1704
Size: 51 136 3 3 1511
Fragments arranged by size:
1511 136 51 3 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 842 1433 1704
Size: 842 591 271
Fragments arranged by size:
842 591 271
BsiHKAI G_wGCw'C
Cuts at: 0 1043 1142 1519 1704
Size: 1043 99 377 185
Fragments arranged by size:
1043 377 185 99
BsmI GAATG_Cn'
Cuts at: 0 1377 1704
Size: 1377 327
Bsp1286I G_dGCh'C
Cuts at: 0 1043 1142 1519 1704
Size: 1043 99 377 185
Fragments arranged by size:
1043 377 185 99
BspHI T'CATG_A
Cuts at: 0 20 827 1124 1704
Size: 20 807 297 580
Fragments arranged by size:
807 580 297 20
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 906 920 1704
Size: 906 14 784
Fragments arranged by size:
906 784 14
BsrFI r'CCGG_y
Cuts at: 0 8 1704
Size: 8 1696
BstYI r'GATC_y
Cuts at: 0 486 969 1158 1704
Size: 486 483 189 546
Fragments arranged by size:
546 486 483 189
BstZ17I GTA'TAC
Cuts at: 0 582 1704
Size: 582 1122
BtgI C'CryG_G
Cuts at: 0 996 1034 1205 1571 1704
Size: 996 38 171 366 133
Fragments arranged by size:
996 366 171 133 38
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1040 1075 1704
Size: 1040 35 629
Fragments arranged by size:
1040 629 35
EaeI y'GGCC_r
Cuts at: 0 1364 1704
Size: 1364 340
EarI CTCTTCn'nnn_
Cuts at: 0 1259 1349 1529 1704
Size: 1259 90 180 175
Fragments arranged by size:
1259 180 175 90
EcoICRI GAG'CTC
Cuts at: 0 1140 1704
Size: 1140 564
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 305 1553 1704
Size: 305 1248 151
Fragments arranged by size:
1248 305 151
EcoO109I rG'GnC_Cy
Cuts at: 0 1443 1704
Size: 1443 261
EcoRI G'AATT_C
Cuts at: 0 679 1704
Size: 679 1025
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 43 75 774 806 1513 1545 1704
Size: 43 32 699 32 707 32 159
Fragments arranged by size:
707 699 159 43 32 32 32
HincII GTy'rAC
Cuts at: 0 521 1704
Size: 521 1183
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 207 317 464 1704
Size: 207 110 147 1240
Fragments arranged by size:
1240 207 147 110
MscI TGG'CCA
Cuts at: 0 1366 1704
Size: 1366 338
MslI CAynn'nnrTG
Cuts at: 0 1021 1052 1129 1259 1339 1366 1704
Size: 1021 31 77 130 80 27 338
Fragments arranged by size:
1021 338 130 80 77 31 27
NcoI C'CATG_G
Cuts at: 0 1205 1704
Size: 1205 499
NheI G'CTAG_C
Cuts at: 0 589 1704
Size: 589 1115
NspI r_CATG'y
Cuts at: 0 855 990 1057 1660 1704
Size: 855 135 67 603 44
Fragments arranged by size:
855 603 135 67 44
PciI A'CATG_T
Cuts at: 0 1656 1704
Size: 1656 48
PpuMI rG'GwC_Cy
Cuts at: 0 1443 1704
Size: 1443 261
PsiI TTA'TAA
Cuts at: 0 629 1704
Size: 629 1075
PstI C_TGCA'G
Cuts at: 0 917 1704
Size: 917 787
SacI G_AGCT'C
Cuts at: 0 1142 1704
Size: 1142 562
SapI GCTCTTCn'nnn_
Cuts at: 0 1529 1704
Size: 1529 175
SbfI CC_TGCA'GG
Cuts at: 0 917 1704
Size: 917 787
SfcI C'TryA_G
Cuts at: 0 470 913 1704
Size: 470 443 791
Fragments arranged by size:
791 470 443
SmaI CCC'GGG
Cuts at: 0 39 1704
Size: 39 1665
SmlI C'TyrA_G
Cuts at: 0 1068 1704
Size: 1068 636
SspI AAT'ATT
Cuts at: 0 1389 1704
Size: 1389 315
StyI C'CwwG_G
Cuts at: 0 239 715 893 1058 1205 1229 1704
Size: 239 476 178 165 147 24 475
Fragments arranged by size:
476 475 239 178 165 147 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1064 1704
Size: 1064 640
TatI w'GTAC_w
Cuts at: 0 926 1704
Size: 926 778
Tth111I GACn'n_nGTC
Cuts at: 0 331 1704
Size: 331 1373
XbaI T'CTAG_A
Cuts at: 0 415 1704
Size: 415 1289
XmaI C'CCGG_G
Cuts at: 0 37 1704
Size: 37 1667
XmnI GAAnn'nnTTC
Cuts at: 0 125 1704
Size: 125 1579
Enzymes that do cut:
AccI AcuI AflIII AlfI AlwNI ApoI AseI AvaI
AvrII BamHI BanII BbsI BglII BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaBI BsaXI BseRI BsgI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrFI BstYI BstZ17I BtgI
CspCI EaeI EarI EcoICRI Eco57MI EcoO109I EcoRI Hin4I
HincII MmeI MscI MslI NcoI NheI NspI PciI
PpuMI PsiI PstI SacI SapI SbfI SfcI SmaI
SmlI SspI StyI TaqII TatI Tth111I XbaI XmaI
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AloI ApaI ApaLI AscI AsiSI BaeI BanI
BbeI BbvCI BcgI BciVI BclI BfrBI BglI BlpI
Bme1580I BplI Bpu10I BsaAI BsaHI BsaWI BseYI BsiEI
BsiWI BsmBI BspEI BsrBI BsrDI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI Bsu36I BtgZI BtsI ClaI
DraI DraIII DrdI EagI EciI EcoNI EcoRV FalI
FseI FspI FspAI HaeII HindIII HpaI KasI KpnI
MfeI MluI MspA1I NaeI NarI NdeI NgoMIV NotI
NruI NsiI PacI PasI PflMI PfoI PmeI PmlI
PpiI PshAI PspOMI PspXI PsrI PvuI PvuII RsrII
SacII SalI SanDI ScaI SexAI SfiI SfoI SgfI
SgrAI SnaBI SpeI SphI SrfI StuI SwaI TstI
XcmI XhoI ZraI