Restriction map for
pFN21AE3244
(Linear) (Six-base) MAPSORT of: KIEE3244.seq Check: 9782 from: 1 to: 1701
KIEE3244.orf
With 182 enzymes: SgfI *
July 3, 2012 17:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1545 1701
Size: 1545 156
AfeI AGC'GCT
Cuts at: 0 313 1701
Size: 313 1388
AflII C'TTAA_G
Cuts at: 0 1083 1701
Size: 1083 618
AlwNI CAG_nnn'CTG
Cuts at: 0 410 1701
Size: 410 1291
ApaI G_GGCC'C
Cuts at: 0 1578 1586 1701
Size: 1578 8 115
Fragments arranged by size:
1578 115 8
ApoI r'AATT_y
Cuts at: 0 541 715 986 1099 1199 1701
Size: 541 174 271 113 100 502
Fragments arranged by size:
541 502 271 174 113 100
AseI AT'TA_AT
Cuts at: 0 584 658 1701
Size: 584 74 1043
Fragments arranged by size:
1043 584 74
AvaI C'yCGr_G
Cuts at: 0 815 1701
Size: 815 886
BamHI G'GATC_C
Cuts at: 0 380 1701
Size: 380 1321
BanI G'GyrC_C
Cuts at: 0 21 59 250 473 1176 1701
Size: 21 38 191 223 703 525
Fragments arranged by size:
703 525 223 191 38 21
BanII G_rGCy'C
Cuts at: 0 31 275 1578 1586 1701
Size: 31 244 1303 8 115
Fragments arranged by size:
1303 244 115 31 8
BbeI G_GCGC'C
Cuts at: 0 25 254 1701
Size: 25 229 1447
Fragments arranged by size:
1447 229 25
BciVI GTATCCnnnnn_n'
Cuts at: 0 1066 1342 1701
Size: 1066 276 359
Fragments arranged by size:
1066 359 276
Bme1580I G_kGCm'C
Cuts at: 0 62 398 1578 1586 1701
Size: 62 336 1180 8 115
Fragments arranged by size:
1180 336 115 62 8
BmrI ACTGGGnnnn_n'
Cuts at: 0 1362 1701
Size: 1362 339
BmtI G_CTAG'C
Cuts at: 0 628 1701
Size: 628 1073
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 1001 1658 1701
Size: 109 892 657 43
Fragments arranged by size:
892 657 109 43
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 1701
Size: 322 1379
BsaHI Gr'CG_yC
Cuts at: 0 22 251 362 1701
Size: 22 229 111 1339
Fragments arranged by size:
1339 229 111 22
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 747 777 1701
Size: 747 30 924
Fragments arranged by size:
924 747 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 755 1701
Size: 755 946
BseYI C'CCAG_C
Cuts at: 0 1315 1701
Size: 1315 386
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 423 1572 1701
Size: 130 293 1149 129
Fragments arranged by size:
1149 293 130 129
BsiEI CG_ry'CG
Cuts at: 0 19 133 1701
Size: 19 114 1568
Fragments arranged by size:
1568 114 19
BsiHKAI G_wGCw'C
Cuts at: 0 85 1467 1540 1701
Size: 85 1382 73 161
Fragments arranged by size:
1382 161 85 73
Bsp1286I G_dGCh'C
Cuts at: 0 31 62 85 275 398 1467 1540 1578
Size: 31 31 23 190 123 1069 73 38
Cuts at: 1578 1586 1701
Size: 8 115
Fragments arranged by size:
1069 190 123 115 73 38 31 31
23 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 990 1545 1701
Size: 990 555 156
Fragments arranged by size:
990 555 156
BsrDI GCAATG_nn'
Cuts at: 0 1625 1701
Size: 1625 76
BsrFI r'CCGG_y
Cuts at: 0 18 96 137 1701
Size: 18 78 41 1564
Fragments arranged by size:
1564 78 41 18
BssHII G'CGCG_C
Cuts at: 0 230 1701
Size: 230 1471
BstAPI GCAn_nnn'nTGC
Cuts at: 0 243 473 1005 1701
Size: 243 230 532 696
Fragments arranged by size:
696 532 243 230
BstBI TT'CG_AA
Cuts at: 0 1497 1701
Size: 1497 204
BstXI CCAn_nnnn'nTGG
Cuts at: 0 357 957 1701
Size: 357 600 744
Fragments arranged by size:
744 600 357
BstYI r'GATC_y
Cuts at: 0 380 722 1701
Size: 380 342 979
Fragments arranged by size:
979 380 342
Bsu36I CC'TnA_GG
Cuts at: 0 295 1701
Size: 295 1406
BtsI GCAGTG_nn'
Cuts at: 0 436 652 1309 1701
Size: 436 216 657 392
Fragments arranged by size:
657 436 392 216
ClaI AT'CG_AT
Cuts at: 0 1044 1701
Size: 1044 657
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1538 1573 1701
Size: 1538 35 128
Fragments arranged by size:
1538 128 35
DraI TTT'AAA
Cuts at: 0 515 1701
Size: 515 1186
DrdI GACnn_nn'nnGTC
Cuts at: 0 352 1701
Size: 352 1349
EaeI y'GGCC_r
Cuts at: 0 16 668 1701
Size: 16 652 1033
Fragments arranged by size:
1033 652 16
EagI C'GGCC_G
Cuts at: 0 16 1701
Size: 16 1685
EarI CTCTTCn'nnn_
Cuts at: 0 800 1701
Size: 800 901
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 1001 1658 1701
Size: 109 892 657 43
Fragments arranged by size:
892 657 109 43
EcoNI CCTnn'n_nnAGG
Cuts at: 0 298 1701
Size: 298 1403
EcoO109I rG'GnC_Cy
Cuts at: 0 42 354 855 1582 1583 1701
Size: 42 312 501 727 1 118
Fragments arranged by size:
727 501 312 118 42 1
EcoRI G'AATT_C
Cuts at: 0 541 1099 1199 1701
Size: 541 558 100 502
Fragments arranged by size:
558 541 502 100
FspI TGC'GCA
Cuts at: 0 998 1701
Size: 998 703
HaeII r_GCGC'y
Cuts at: 0 25 254 315 1701
Size: 25 229 61 1386
Fragments arranged by size:
1386 229 61 25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1386 1418 1645 1677 1701
Size: 1386 32 227 32 24
Fragments arranged by size:
1386 227 32 32 24
HincII GTy'rAC
Cuts at: 0 442 1701
Size: 442 1259
HindIII A'AGCT_T
Cuts at: 0 448 634 1701
Size: 448 186 1067
Fragments arranged by size:
1067 448 186
KasI G'GCGC_C
Cuts at: 0 21 250 1701
Size: 21 229 1451
Fragments arranged by size:
1451 229 21
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1107 1419 1701
Size: 1107 312 282
Fragments arranged by size:
1107 312 282
MscI TGG'CCA
Cuts at: 0 670 1701
Size: 670 1031
MslI CAynn'nnrTG
Cuts at: 0 1418 1701
Size: 1418 283
MspA1I CmG'CkG
Cuts at: 0 129 144 1701
Size: 129 15 1557
Fragments arranged by size:
1557 129 15
NaeI GCC'GGC
Cuts at: 0 20 139 1701
Size: 20 119 1562
Fragments arranged by size:
1562 119 20
NarI GG'CG_CC
Cuts at: 0 22 251 1701
Size: 22 229 1450
Fragments arranged by size:
1450 229 22
NgoMIV G'CCGG_C
Cuts at: 0 18 137 1701
Size: 18 119 1564
Fragments arranged by size:
1564 119 18
NheI G'CTAG_C
Cuts at: 0 624 1701
Size: 624 1077
NspI r_CATG'y
Cuts at: 0 1618 1701
Size: 1618 83
PfoI T'CCnGG_A
Cuts at: 0 356 857 1701
Size: 356 501 844
Fragments arranged by size:
844 501 356
PpuMI rG'GwC_Cy
Cuts at: 0 354 855 1701
Size: 354 501 846
Fragments arranged by size:
846 501 354
PshAI GACnn'nnGTC
Cuts at: 0 1421 1701
Size: 1421 280
PspOMI G'GGCC_C
Cuts at: 0 1574 1582 1701
Size: 1574 8 119
Fragments arranged by size:
1574 119 8
PspXI vC'TCGA_Gb
Cuts at: 0 815 1701
Size: 815 886
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1333 1365 1701
Size: 1333 32 336
Fragments arranged by size:
1333 336 32
PstI C_TGCA'G
Cuts at: 0 248 1369 1701
Size: 248 1121 332
Fragments arranged by size:
1121 332 248
PvuII CAG'CTG
Cuts at: 0 144 1701
Size: 144 1557
ScaI AGT'ACT
Cuts at: 0 821 1668 1701
Size: 821 847 33
Fragments arranged by size:
847 821 33
SfcI C'TryA_G
Cuts at: 0 244 845 1365 1701
Size: 244 601 520 336
Fragments arranged by size:
601 520 336 244
SfoI GGC'GCC
Cuts at: 0 23 252 1701
Size: 23 229 1449
Fragments arranged by size:
1449 229 23
SmlI C'TyrA_G
Cuts at: 0 337 815 1083 1701
Size: 337 478 268 618
Fragments arranged by size:
618 478 337 268
StyI C'CwwG_G
Cuts at: 0 350 1094 1570 1578 1701
Size: 350 744 476 8 123
Fragments arranged by size:
744 476 350 123 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 75 1701
Size: 75 1626
TatI w'GTAC_w
Cuts at: 0 486 790 819 899 1666 1701
Size: 486 304 29 80 767 35
Fragments arranged by size:
767 486 304 80 35 29
XhoI C'TCGA_G
Cuts at: 0 815 1701
Size: 815 886
Enzymes that do cut:
AarI AfeI AflII AlwNI ApaI ApoI AseI AvaI
BamHI BanI BanII BbeI BciVI Bme1580I BmrI BmtI
BpmI BpuEI BsaHI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspMI BsrDI BsrFI BssHII BstAPI BstBI
BstXI BstYI Bsu36I BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII KasI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NheI NspI PfoI PpuMI
PshAI PspOMI PspXI PsrI PstI PvuII ScaI SfcI
SfoI SmlI StyI TaqII TatI XhoI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlfI AloI ApaLI AscI AsiSI AvrII BaeI
BbsI BbvCI BcgI BclI BfrBI BglI BglII BlpI
BmgBI BplI Bpu10I BsaI BsaAI BsaBI BsaWI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BsrGI BssSI BstEII
BstZ17I BtgI BtgZI DraIII EciI EcoICRI EcoRV FalI
FseI FspAI HpaI KpnI MfeI MluI NcoI NdeI
NotI NruI NsiI PacI PasI PciI PflMI PmeI
PmlI PpiI PsiI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI SexAI SfiI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI TstI
Tth111I XbaI XcmI XmaI XmnI ZraI