Restriction map for
pFN21AE3243
(Linear) (Six-base) MAPSORT of: KIEE3243.seq Check: 5365 from: 1 to: 1698
KIEE3243.orf
With 182 enzymes: SgfI *
March 22, 2012 16:27 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 793 1504 1698
Size: 793 711 194
Fragments arranged by size:
793 711 194
AflII C'TTAA_G
Cuts at: 0 424 1698
Size: 424 1274
AhdI GACnn_n'nnGTC
Cuts at: 0 444 1698
Size: 444 1254
AlwNI CAG_nnn'CTG
Cuts at: 0 687 729 1234 1698
Size: 687 42 505 464
Fragments arranged by size:
687 505 464 42
ApaLI G'TGCA_C
Cuts at: 0 1597 1698
Size: 1597 101
ApoI r'AATT_y
Cuts at: 0 762 1698
Size: 762 936
AvaI C'yCGr_G
Cuts at: 0 753 1490 1698
Size: 753 737 208
Fragments arranged by size:
753 737 208
BanI G'GyrC_C
Cuts at: 0 234 793 1630 1698
Size: 234 559 837 68
Fragments arranged by size:
837 559 234 68
BbsI GAAGACnn'nnnn_
Cuts at: 0 1423 1698
Size: 1423 275
BbvCI CC'TCA_GC
Cuts at: 0 95 691 1013 1698
Size: 95 596 322 685
Fragments arranged by size:
685 596 322 95
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1501 1535 1698
Size: 1501 34 163
Fragments arranged by size:
1501 163 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1082 1698
Size: 1082 616
Bme1580I G_kGCm'C
Cuts at: 0 1601 1698
Size: 1601 97
BmgBI CAC'GTC
Cuts at: 0 171 1698
Size: 171 1527
BmrI ACTGGGnnnn_n'
Cuts at: 0 554 692 1698
Size: 554 138 1006
Fragments arranged by size:
1006 554 138
BmtI G_CTAG'C
Cuts at: 0 742 1698
Size: 742 956
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 751 773 1225 1698
Size: 751 22 452 473
Fragments arranged by size:
751 473 452 22
Bpu10I CC'TnA_GC
Cuts at: 0 95 691 1013 1698
Size: 95 596 322 685
Fragments arranged by size:
685 596 322 95
BsaI GGTCTCn'nnnn_
Cuts at: 0 1670 1698
Size: 1670 28
BsaAI yAC'GTr
Cuts at: 0 1596 1698
Size: 1596 102
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 266 296 432 462 1054 1084 1698
Size: 266 30 136 30 592 30 614
Fragments arranged by size:
614 592 266 136 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1075 1273 1621 1698
Size: 1075 198 348 77
Fragments arranged by size:
1075 348 198 77
BseYI C'CCAG_C
Cuts at: 0 66 1321 1698
Size: 66 1255 377
Fragments arranged by size:
1255 377 66
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1164 1233 1698
Size: 1164 69 465
Fragments arranged by size:
1164 465 69
BsiHKAI G_wGCw'C
Cuts at: 0 1601 1698
Size: 1601 97
BsmI GAATG_Cn'
Cuts at: 0 951 1698
Size: 951 747
Bsp1286I G_dGCh'C
Cuts at: 0 1601 1698
Size: 1601 97
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1429 1688 1698
Size: 1429 259 10
Fragments arranged by size:
1429 259 10
BsrDI GCAATG_nn'
Cuts at: 0 1389 1698
Size: 1389 309
BsrFI r'CCGG_y
Cuts at: 0 411 929 1698
Size: 411 518 769
Fragments arranged by size:
769 518 411
BssSI C'ACGA_G
Cuts at: 0 1520 1698
Size: 1520 178
BstXI CCAn_nnnn'nTGG
Cuts at: 0 57 1107 1698
Size: 57 1050 591
Fragments arranged by size:
1050 591 57
BstYI r'GATC_y
Cuts at: 0 1688 1698
Size: 1688 10
BtgI C'CryG_G
Cuts at: 0 89 782 1698
Size: 89 693 916
Fragments arranged by size:
916 693 89
BtsI GCAGTG_nn'
Cuts at: 0 1599 1698
Size: 1599 99
EaeI y'GGCC_r
Cuts at: 0 413 1698
Size: 413 1285
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 751 773 793 1225 1504 1698
Size: 751 22 20 432 279 194
Fragments arranged by size:
751 432 279 194 22 20
EcoNI CCTnn'n_nnAGG
Cuts at: 0 772 1698
Size: 772 926
EcoO109I rG'GnC_Cy
Cuts at: 0 660 684 1698
Size: 660 24 1014
Fragments arranged by size:
1014 660 24
EcoRI G'AATT_C
Cuts at: 0 762 1698
Size: 762 936
HaeII r_GCGC'y
Cuts at: 0 1672 1698
Size: 1672 26
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 22 54 430 462 1357 1389 1546 1578
Size: 22 32 376 32 895 32 157 32
Cuts at: 1578 1698
Size: 120
Fragments arranged by size:
895 376 157 120 32 32 32 32
22
HincII GTy'rAC
Cuts at: 0 162 1698
Size: 162 1536
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 871 1698
Size: 871 827
MslI CAynn'nnrTG
Cuts at: 0 344 1549 1698
Size: 344 1205 149
Fragments arranged by size:
1205 344 149
MspA1I CmG'CkG
Cuts at: 0 504 640 1148 1462 1698
Size: 504 136 508 314 236
Fragments arranged by size:
508 504 314 236 136
NaeI GCC'GGC
Cuts at: 0 413 1698
Size: 413 1285
NcoI C'CATG_G
Cuts at: 0 782 1698
Size: 782 916
NdeI CA'TA_TG
Cuts at: 0 716 1698
Size: 716 982
NgoMIV G'CCGG_C
Cuts at: 0 411 1698
Size: 411 1287
NheI G'CTAG_C
Cuts at: 0 738 1698
Size: 738 960
PasI CC'CwG_GG
Cuts at: 0 1626 1698
Size: 1626 72
PfoI T'CCnGG_A
Cuts at: 0 1106 1698
Size: 1106 592
PmlI CAC'GTG
Cuts at: 0 1596 1698
Size: 1596 102
PpuMI rG'GwC_Cy
Cuts at: 0 684 1698
Size: 684 1014
PspXI vC'TCGA_Gb
Cuts at: 0 753 1698
Size: 753 945
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 25 57 1698
Size: 25 32 1641
Fragments arranged by size:
1641 32 25
PstI C_TGCA'G
Cuts at: 0 710 1698
Size: 710 988
PvuII CAG'CTG
Cuts at: 0 504 1462 1698
Size: 504 958 236
Fragments arranged by size:
958 504 236
SfcI C'TryA_G
Cuts at: 0 706 1698
Size: 706 992
SmlI C'TyrA_G
Cuts at: 0 424 753 1698
Size: 424 329 945
Fragments arranged by size:
945 424 329
StyI C'CwwG_G
Cuts at: 0 782 1274 1698
Size: 782 492 424
Fragments arranged by size:
782 492 424
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1618 1698
Size: 1618 80
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 62 94 432 464 1054 1086 1614 1646
Size: 62 32 338 32 590 32 528 32
Cuts at: 1646 1698
Size: 52
Fragments arranged by size:
590 528 338 62 52 32 32 32
32
XbaI T'CTAG_A
Cuts at: 0 309 1698
Size: 309 1389
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 226 1104 1698
Size: 226 878 594
Fragments arranged by size:
878 594 226
XhoI C'TCGA_G
Cuts at: 0 753 1698
Size: 753 945
Enzymes that do cut:
AcuI AflII AhdI AlwNI ApaLI ApoI AvaI BanI
BbsI BbvCI BcgI BciVI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BsaI BsaAI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspMI BsrDI BsrFI BssSI BstXI
BstYI BtgI BtsI EaeI Eco57MI EcoNI EcoO109I EcoRI
HaeII Hin4I HincII MmeI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI PasI PfoI PmlI PpuMI PspXI
PsrI PstI PvuII SfcI SmlI StyI TaqII TstI
XbaI XcmI XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflIII AgeI
AleI AlfI AloI ApaI AscI AseI AsiSI AvrII
BaeI BamHI BanII BbeI BclI BfrBI BglI BglII
BlpI BplI BpuEI BsaBI BsaHI BsaWI BsiEI BsiWI
BsmBI BspEI BspHI BsrBI BsrGI BssHII BstAPI BstBI
BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
DrdI EagI EarI EciI EcoICRI EcoRV FalI FseI
FspI FspAI HindIII HpaI KasI KpnI MfeI MluI
MscI NarI NotI NruI NsiI NspI PacI PciI
PflMI PmeI PpiI PshAI PsiI PspOMI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI TatI Tth111I XmaI XmnI
ZraI