Restriction map for
pFN21AE3241
(Linear) (Six-base) MAPSORT of: KIEE3241.seq Check: 5904 from: 1 to: 1689
KIEE3241.orf
With 182 enzymes: SgfI *
March 22, 2012 16:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 254 929 1689
Size: 254 675 760
Fragments arranged by size:
760 675 254
AccI GT'mk_AC
Cuts at: 0 941 1436 1689
Size: 941 495 253
Fragments arranged by size:
941 495 253
Acc65I G'GTAC_C
Cuts at: 0 827 1689
Size: 827 862
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 561 697 1231 1689
Size: 561 136 534 458
Fragments arranged by size:
561 534 458 136
AflIII A'CryG_T
Cuts at: 0 1595 1689
Size: 1595 94
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 550 582 1689
Size: 550 32 1107
Fragments arranged by size:
1107 550 32
AlwNI CAG_nnn'CTG
Cuts at: 0 342 444 1689
Size: 342 102 1245
Fragments arranged by size:
1245 342 102
ApoI r'AATT_y
Cuts at: 0 1510 1522 1689
Size: 1510 12 167
Fragments arranged by size:
1510 167 12
AvaI C'yCGr_G
Cuts at: 0 53 89 958 1081 1689
Size: 53 36 869 123 608
Fragments arranged by size:
869 608 123 53 36
BanI G'GyrC_C
Cuts at: 0 827 1462 1689
Size: 827 635 227
Fragments arranged by size:
827 635 227
BanII G_rGCy'C
Cuts at: 0 263 434 1088 1182 1235 1651 1689
Size: 263 171 654 94 53 416 38
Fragments arranged by size:
654 416 263 171 94 53 38
BbvCI CC'TCA_GC
Cuts at: 0 160 1689
Size: 160 1529
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 729 763 1689
Size: 729 34 926
Fragments arranged by size:
926 729 34
BglI GCCn_nnn'nGGC
Cuts at: 0 460 1689
Size: 460 1229
Bme1580I G_kGCm'C
Cuts at: 0 1353 1689
Size: 1353 336
BmgBI CAC'GTC
Cuts at: 0 1458 1689
Size: 1458 231
BmrI ACTGGGnnnn_n'
Cuts at: 0 330 1689
Size: 330 1359
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 277 289 934 1312 1398 1408 1635
Size: 187 90 12 645 378 86 10 227
Cuts at: 1635 1689
Size: 54
Fragments arranged by size:
645 378 227 187 90 86 54 12
10
Bpu10I CC'TnA_GC
Cuts at: 0 160 1689
Size: 160 1529
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 937 1689
Size: 100 837 752
Fragments arranged by size:
837 752 100
BsaI GGTCTCn'nnnn_
Cuts at: 0 13 1324 1689
Size: 13 1311 365
Fragments arranged by size:
1311 365 13
BsaBI GATnn'nnATC
Cuts at: 0 802 1689
Size: 802 887
BsaWI w'CCGG_w
Cuts at: 0 992 1217 1689
Size: 992 225 472
Fragments arranged by size:
992 472 225
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 550 580 1096 1126 1178 1208 1689
Size: 550 30 516 30 52 30 481
Fragments arranged by size:
550 516 481 52 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 865 1114 1573 1647 1689
Size: 865 249 459 74 42
Fragments arranged by size:
865 459 249 74 42
BseYI C'CCAG_C
Cuts at: 0 545 1393 1689
Size: 545 848 296
Fragments arranged by size:
848 545 296
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 907 1689
Size: 907 782
BsiEI CG_ry'CG
Cuts at: 0 635 1689
Size: 635 1054
BsiHKAI G_wGCw'C
Cuts at: 0 263 920 1088 1689
Size: 263 657 168 601
Fragments arranged by size:
657 601 263 168
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1564 1689
Size: 1564 125
Bsp1286I G_dGCh'C
Cuts at: 0 263 434 920 1088 1182 1235 1353 1651
Size: 263 171 486 168 94 53 118 298
Cuts at: 1651 1689
Size: 38
Fragments arranged by size:
486 298 263 171 168 118 94 53
38
BspEI T'CCGG_A
Cuts at: 0 992 1689
Size: 992 697
BspHI T'CATG_A
Cuts at: 0 806 1689
Size: 806 883
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 254 731 929 1342 1689
Size: 254 477 198 413 347
Fragments arranged by size:
477 413 347 254 198
BsrDI GCAATG_nn'
Cuts at: 0 993 1689
Size: 993 696
BsrFI r'CCGG_y
Cuts at: 0 249 634 1689
Size: 249 385 1055
Fragments arranged by size:
1055 385 249
BssHII G'CGCG_C
Cuts at: 0 876 1689
Size: 876 813
BstEII G'GTnAC_C
Cuts at: 0 1137 1689
Size: 1137 552
BstYI r'GATC_y
Cuts at: 0 419 995 1689
Size: 419 576 694
Fragments arranged by size:
694 576 419
Bsu36I CC'TnA_GG
Cuts at: 0 514 1689
Size: 514 1175
BtgI C'CryG_G
Cuts at: 0 23 551 1016 1115 1182 1689
Size: 23 528 465 99 67 507
Fragments arranged by size:
528 507 465 99 67 23
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 30 1689
Size: 30 1659
BtsI GCAGTG_nn'
Cuts at: 0 532 1689
Size: 532 1157
DrdI GACnn_nn'nnGTC
Cuts at: 0 580 988 1689
Size: 580 408 701
Fragments arranged by size:
701 580 408
EaeI y'GGCC_r
Cuts at: 0 29 107 1013 1160 1689
Size: 29 78 906 147 529
Fragments arranged by size:
906 529 147 78 29
EarI CTCTTCn'nnn_
Cuts at: 0 367 439 676 889 1366 1689
Size: 367 72 237 213 477 323
Fragments arranged by size:
477 367 323 237 213 72
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 948 1689
Size: 948 741
EcoICRI GAG'CTC
Cuts at: 0 261 1086 1689
Size: 261 825 603
Fragments arranged by size:
825 603 261
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 277 289 561 697 934 1231 1312
Size: 187 90 12 272 136 237 297 81
Cuts at: 1312 1398 1408 1635 1689
Size: 86 10 227 54
Fragments arranged by size:
297 272 237 227 187 136 90 86
81 54 12 10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 379 448 1363 1689
Size: 379 69 915 326
Fragments arranged by size:
915 379 326 69
EcoO109I rG'GnC_Cy
Cuts at: 0 409 582 1061 1168 1299 1368 1689
Size: 409 173 479 107 131 69 321
Fragments arranged by size:
479 409 321 173 131 107 69
EcoRI G'AATT_C
Cuts at: 0 1510 1689
Size: 1510 179
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 228 1689
Size: 228 1461
HaeII r_GCGC'y
Cuts at: 0 1288 1314 1605 1689
Size: 1288 26 291 84
Fragments arranged by size:
1288 291 84 26
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 27 806 838 1689
Size: 27 779 32 851
Fragments arranged by size:
851 779 32 27
KpnI G_GTAC'C
Cuts at: 0 831 1689
Size: 831 858
MscI TGG'CCA
Cuts at: 0 31 109 1689
Size: 31 78 1580
Fragments arranged by size:
1580 78 31
MspA1I CmG'CkG
Cuts at: 0 129 1386 1689
Size: 129 1257 303
Fragments arranged by size:
1257 303 129
NaeI GCC'GGC
Cuts at: 0 251 1689
Size: 251 1438
NcoI C'CATG_G
Cuts at: 0 551 1689
Size: 551 1138
NgoMIV G'CCGG_C
Cuts at: 0 249 1689
Size: 249 1440
PasI CC'CwG_GG
Cuts at: 0 1131 1689
Size: 1131 558
PflMI CCAn_nnn'nTGG
Cuts at: 0 342 552 1550 1689
Size: 342 210 998 139
Fragments arranged by size:
998 342 210 139
PfoI T'CCnGG_A
Cuts at: 0 374 422 485 569 866 1689
Size: 374 48 63 84 297 823
Fragments arranged by size:
823 374 297 84 63 48
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 550 582 1689
Size: 550 32 1107
Fragments arranged by size:
1107 550 32
PpuMI rG'GwC_Cy
Cuts at: 0 582 1061 1168 1299 1368 1689
Size: 582 479 107 131 69 321
Fragments arranged by size:
582 479 321 131 107 69
PshAI GACnn'nnGTC
Cuts at: 0 788 1689
Size: 788 901
PstI C_TGCA'G
Cuts at: 0 370 452 527 1094 1689
Size: 370 82 75 567 595
Fragments arranged by size:
595 567 370 82 75
PvuII CAG'CTG
Cuts at: 0 1386 1689
Size: 1386 303
SacI G_AGCT'C
Cuts at: 0 263 1088 1689
Size: 263 825 601
Fragments arranged by size:
825 601 263
SanDI GG'GwC_CC
Cuts at: 0 1061 1689
Size: 1061 628
SapI GCTCTTCn'nnn_
Cuts at: 0 439 1689
Size: 439 1250
SbfI CC_TGCA'GG
Cuts at: 0 1094 1689
Size: 1094 595
SfcI C'TryA_G
Cuts at: 0 366 448 523 1090 1689
Size: 366 82 75 567 599
Fragments arranged by size:
599 567 366 82 75
SmaI CCC'GGG
Cuts at: 0 91 1689
Size: 91 1598
SmlI C'TyrA_G
Cuts at: 0 115 952 1689
Size: 115 837 737
Fragments arranged by size:
837 737 115
StyI C'CwwG_G
Cuts at: 0 551 1005 1046 1689
Size: 551 454 41 643
Fragments arranged by size:
643 551 454 41
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1450 1689
Size: 1450 239
TatI w'GTAC_w
Cuts at: 0 506 732 1689
Size: 506 226 957
Fragments arranged by size:
957 506 226
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1178 1210 1689
Size: 1178 32 479
Fragments arranged by size:
1178 479 32
XmaI C'CCGG_G
Cuts at: 0 89 1689
Size: 89 1600
XmnI GAAnn'nnTTC
Cuts at: 0 1514 1689
Size: 1514 175
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AloI AlwNI ApoI
AvaI BanI BanII BbvCI BcgI BglI Bme1580I BmgBI
BmrI BpmI Bpu10I BpuEI BsaI BsaBI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrDI BsrFI BssHII BstEII BstYI Bsu36I
BtgI BtgZI BtsI DrdI EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I KpnI
MscI MspA1I NaeI NcoI NgoMIV PasI PflMI PfoI
PpiI PpuMI PshAI PstI PvuII SacI SanDI SapI
SbfI SfcI SmaI SmlI StyI TaqII TatI TstI
XmaI XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AgeI AhdI AleI AlfI
ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BbsI BciVI BclI BfrBI BglII BlpI BmtI
BplI BsaAI BsaHI BsiWI BsmI BsrBI BsrGI BssSI
BstAPI BstBI BstXI BstZ17I ClaI CspCI DraI DraIII
EagI EcoRV FseI FspI FspAI HincII HindIII HpaI
KasI MfeI MluI MmeI MslI NarI NdeI NheI
NotI NruI NsiI NspI PacI PciI PmeI PmlI
PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI
ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI StuI SwaI Tth111I XbaI XcmI
XhoI ZraI