Restriction map for
pFN21AE3240
(Linear) (Six-base) MAPSORT of: KIEE3240.seq Check: 8299 from: 1 to: 1686
KIEE3240.orf
With 182 enzymes: SgfI *
March 22, 2012 16:26 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 784 1351 1686
Size: 784 567 335
Fragments arranged by size:
784 567 335
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 860 1686
Size: 860 826
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 904 936 1686
Size: 904 32 750
Fragments arranged by size:
904 750 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1042 1255 1686
Size: 1042 213 431
Fragments arranged by size:
1042 431 213
AvaI C'yCGr_G
Cuts at: 0 1354 1391 1686
Size: 1354 37 295
Fragments arranged by size:
1354 295 37
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 161 194 1686
Size: 161 33 1492
Fragments arranged by size:
1492 161 33
BanI G'GyrC_C
Cuts at: 0 1302 1686
Size: 1302 384
BanII G_rGCy'C
Cuts at: 0 881 1229 1328 1686
Size: 881 348 99 358
Fragments arranged by size:
881 358 348 99
BbsI GAAGACnn'nnnn_
Cuts at: 0 199 1686
Size: 199 1487
BbvCI CC'TCA_GC
Cuts at: 0 47 1686
Size: 47 1639
BciVI GTATCCnnnnn_n'
Cuts at: 0 162 1686
Size: 162 1524
BclI T'GATC_A
Cuts at: 0 1271 1686
Size: 1271 415
BglI GCCn_nnn'nGGC
Cuts at: 0 1345 1686
Size: 1345 341
Bme1580I G_kGCm'C
Cuts at: 0 1307 1686
Size: 1307 379
BmrI ACTGGGnnnn_n'
Cuts at: 0 151 1686
Size: 151 1535
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 587 1040 1686
Size: 587 453 646
Fragments arranged by size:
646 587 453
Bpu10I CC'TnA_GC
Cuts at: 0 47 1686
Size: 47 1639
BsaI GGTCTCn'nnnn_
Cuts at: 0 1289 1427 1686
Size: 1289 138 259
Fragments arranged by size:
1289 259 138
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 186 216 1286 1316 1686
Size: 186 30 1070 30 370
Fragments arranged by size:
1070 370 186 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 373 1686
Size: 373 1313
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 791 1647 1686
Size: 791 856 39
Fragments arranged by size:
856 791 39
BsiEI CG_ry'CG
Cuts at: 0 903 1686
Size: 903 783
BsiHKAI G_wGCw'C
Cuts at: 0 1229 1328 1686
Size: 1229 99 358
Fragments arranged by size:
1229 358 99
BsmI GAATG_Cn'
Cuts at: 0 846 1686
Size: 846 840
Bsp1286I G_dGCh'C
Cuts at: 0 881 1229 1307 1328 1686
Size: 881 348 78 21 358
Fragments arranged by size:
881 358 348 78 21
BspHI T'CATG_A
Cuts at: 0 884 1686
Size: 884 802
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 970 1686
Size: 970 716
BsrFI r'CCGG_y
Cuts at: 0 287 1686
Size: 287 1399
BstYI r'GATC_y
Cuts at: 0 96 229 330 509 1039 1686
Size: 96 133 101 179 530 647
Fragments arranged by size:
647 530 179 133 101 96
Bsu36I CC'TnA_GG
Cuts at: 0 1308 1686
Size: 1308 378
BtgI C'CryG_G
Cuts at: 0 1340 1686
Size: 1340 346
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 920 1524 1686
Size: 920 604 162
Fragments arranged by size:
920 604 162
DraIII CAC_nnn'GTG
Cuts at: 0 1175 1177 1686
Size: 1175 2 509
Fragments arranged by size:
1175 509 2
EaeI y'GGCC_r
Cuts at: 0 1346 1686
Size: 1346 340
EarI CTCTTCn'nnn_
Cuts at: 0 629 1406 1686
Size: 629 777 280
Fragments arranged by size:
777 629 280
EcoICRI GAG'CTC
Cuts at: 0 1227 1326 1686
Size: 1227 99 360
Fragments arranged by size:
1227 360 99
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 587 784 1040 1351 1686
Size: 587 197 256 311 335
Fragments arranged by size:
587 335 311 256 197
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1234 1333 1686
Size: 1234 99 353
Fragments arranged by size:
1234 353 99
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 213 1686
Size: 213 1473
HaeII r_GCGC'y
Cuts at: 0 1551 1686
Size: 1551 135
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 186 218 383 415 1024 1056 1559 1591
Size: 186 32 165 32 609 32 503 32
Cuts at: 1591 1686
Size: 95
Fragments arranged by size:
609 503 186 165 95 32 32 32
32
HincII GTy'rAC
Cuts at: 0 945 1686
Size: 945 741
MscI TGG'CCA
Cuts at: 0 1348 1686
Size: 1348 338
MslI CAynn'nnrTG
Cuts at: 0 484 934 1579 1686
Size: 484 450 645 107
Fragments arranged by size:
645 484 450 107
NcoI C'CATG_G
Cuts at: 0 1340 1686
Size: 1340 346
PflMI CCAn_nnn'nTGG
Cuts at: 0 211 427 1686
Size: 211 216 1259
Fragments arranged by size:
1259 216 211
PfoI T'CCnGG_A
Cuts at: 0 420 866 1686
Size: 420 446 820
Fragments arranged by size:
820 446 420
PstI C_TGCA'G
Cuts at: 0 348 978 1686
Size: 348 630 708
Fragments arranged by size:
708 630 348
SacI G_AGCT'C
Cuts at: 0 1229 1328 1686
Size: 1229 99 358
Fragments arranged by size:
1229 358 99
SexAI A'CCwGG_T
Cuts at: 0 1298 1686
Size: 1298 388
SfcI C'TryA_G
Cuts at: 0 344 974 1686
Size: 344 630 712
Fragments arranged by size:
712 630 344
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1345 1686
Size: 1345 341
StyI C'CwwG_G
Cuts at: 0 17 1340 1686
Size: 17 1323 346
Fragments arranged by size:
1323 346 17
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 133 1686
Size: 133 1553
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1286 1318 1686
Size: 1286 32 368
Fragments arranged by size:
1286 368 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 24 1686
Size: 24 1662
Enzymes that do cut:
AcuI AlfI AloI AlwNI AvaI BaeI BanI BanII
BbsI BbvCI BciVI BclI BglI Bme1580I BmrI BpmI
Bpu10I BsaI BsaXI BseRI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrFI BstYI Bsu36I BtgI BtgZI
DraIII EaeI EarI EcoICRI Eco57MI EcoNI FalI HaeII
Hin4I HincII MscI MslI NcoI PflMI PfoI PstI
SacI SexAI SfcI SfiI StyI TaqII TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BamHI BbeI BcgI BfrBI BglII BlpI
BmgBI BmtI BplI BpuEI BsaAI BsaBI BsaHI BsaWI
BseYI BsiWI BsmBI BspEI BsrBI BsrDI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstZ17I BtsI ClaI
CspCI DraI DrdI EagI EciI EcoO109I EcoRI EcoRV
FseI FspI FspAI HindIII HpaI KasI KpnI MfeI
MluI MmeI MspA1I NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI PvuII RsrII SacII SalI SanDI SapI SbfI
ScaI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TatI Tth111I XbaI
XhoI XmaI XmnI ZraI