Restriction map for
pFN21AE3239
(Linear) (Six-base) MAPSORT of: KIEE3239.seq Check: 673 from: 1 to: 1677
KIEE3239.orf
With 182 enzymes: SgfI *
March 22, 2012 16:26 ..
AccI GT'mk_AC
Cuts at: 0 853 1677
Size: 853 824
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 590 641 1223 1422 1677
Size: 590 51 582 199 255
Fragments arranged by size:
590 582 255 199 51
AflIII A'CryG_T
Cuts at: 0 892 1139 1677
Size: 892 247 538
Fragments arranged by size:
892 538 247
ApaI G_GGCC'C
Cuts at: 0 17 1677
Size: 17 1660
ApoI r'AATT_y
Cuts at: 0 880 1327 1677
Size: 880 447 350
Fragments arranged by size:
880 447 350
AvaI C'yCGr_G
Cuts at: 0 118 252 292 520 1677
Size: 118 134 40 228 1157
Fragments arranged by size:
1157 228 134 118 40
BanI G'GyrC_C
Cuts at: 0 172 505 1637 1677
Size: 172 333 1132 40
Fragments arranged by size:
1132 333 172 40
BanII G_rGCy'C
Cuts at: 0 17 228 1344 1677
Size: 17 211 1116 333
Fragments arranged by size:
1116 333 211 17
BbeI G_GCGC'C
Cuts at: 0 176 509 1677
Size: 176 333 1168
Fragments arranged by size:
1168 333 176
BbsI GAAGACnn'nnnn_
Cuts at: 0 504 638 1677
Size: 504 134 1039
Fragments arranged by size:
1039 504 134
BbvCI CC'TCA_GC
Cuts at: 0 56 487 1677
Size: 56 431 1190
Fragments arranged by size:
1190 431 56
BglI GCCn_nnn'nGGC
Cuts at: 0 170 329 350 1677
Size: 170 159 21 1327
Fragments arranged by size:
1327 170 159 21
Bme1580I G_kGCm'C
Cuts at: 0 17 1677
Size: 17 1660
BmgBI CAC'GTC
Cuts at: 0 1241 1677
Size: 1241 436
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 47 242 1328 1677
Size: 47 195 1086 349
Fragments arranged by size:
1086 349 195 47
Bpu10I CC'TnA_GC
Cuts at: 0 56 487 1677
Size: 56 431 1190
Fragments arranged by size:
1190 431 56
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1272 1677
Size: 1272 405
BsaHI Gr'CG_yC
Cuts at: 0 67 173 506 1677
Size: 67 106 333 1171
Fragments arranged by size:
1171 333 106 67
BsaWI w'CCGG_w
Cuts at: 0 202 228 1677
Size: 202 26 1449
Fragments arranged by size:
1449 202 26
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1331 1361 1677
Size: 1331 30 316
Fragments arranged by size:
1331 316 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 73 1321 1677
Size: 73 1248 356
Fragments arranged by size:
1248 356 73
BseYI C'CCAG_C
Cuts at: 0 761 1265 1677
Size: 761 504 412
Fragments arranged by size:
761 504 412
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1237 1530 1677
Size: 1237 293 147
Fragments arranged by size:
1237 293 147
BsiEI CG_ry'CG
Cuts at: 0 44 1677
Size: 44 1633
BsiHKAI G_wGCw'C
Cuts at: 0 228 1288 1344 1677
Size: 228 1060 56 333
Fragments arranged by size:
1060 333 228 56
Bsp1286I G_dGCh'C
Cuts at: 0 17 228 1288 1344 1677
Size: 17 211 1060 56 333
Fragments arranged by size:
1060 333 211 56 17
BspEI T'CCGG_A
Cuts at: 0 202 228 1677
Size: 202 26 1449
Fragments arranged by size:
1449 202 26
BspHI T'CATG_A
Cuts at: 0 627 1677
Size: 627 1050
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 688 1677
Size: 688 989
BsrFI r'CCGG_y
Cuts at: 0 284 665 1677
Size: 284 381 1012
Fragments arranged by size:
1012 381 284
BssSI C'ACGA_G
Cuts at: 0 73 1145 1677
Size: 73 1072 532
Fragments arranged by size:
1072 532 73
BstXI CCAn_nnnn'nTGG
Cuts at: 0 25 1265 1677
Size: 25 1240 412
Fragments arranged by size:
1240 412 25
BstYI r'GATC_y
Cuts at: 0 711 1677
Size: 711 966
BstZ17I GTA'TAC
Cuts at: 0 854 1677
Size: 854 823
Bsu36I CC'TnA_GG
Cuts at: 0 1632 1677
Size: 1632 45
BtgI C'CryG_G
Cuts at: 0 399 470 501 1224 1677
Size: 399 71 31 723 453
Fragments arranged by size:
723 453 399 71 31
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 164 1677
Size: 164 1513
BtsI GCAGTG_nn'
Cuts at: 0 1518 1677
Size: 1518 159
EaeI y'GGCC_r
Cuts at: 0 41 1677
Size: 41 1636
EagI C'GGCC_G
Cuts at: 0 41 1677
Size: 41 1636
EarI CTCTTCn'nnn_
Cuts at: 0 1280 1677
Size: 1280 397
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 237 1677
Size: 237 1440
EcoICRI GAG'CTC
Cuts at: 0 226 1342 1677
Size: 226 1116 335
Fragments arranged by size:
1116 335 226
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 47 242 590 641 1223 1328 1422 1677
Size: 47 195 348 51 582 105 94 255
Fragments arranged by size:
582 348 255 195 105 94 51 47
EcoO109I rG'GnC_Cy
Cuts at: 0 14 1677
Size: 14 1663
EcoRI G'AATT_C
Cuts at: 0 1327 1677
Size: 1327 350
EcoRV GAT'ATC
Cuts at: 0 961 1677
Size: 961 716
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 595 900 1058 1677
Size: 595 305 158 619
Fragments arranged by size:
619 595 305 158
HaeII r_GCGC'y
Cuts at: 0 176 362 509 1677
Size: 176 186 147 1168
Fragments arranged by size:
1168 186 176 147
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 317 349 577 609 1551 1583 1677
Size: 317 32 228 32 942 32 94
Fragments arranged by size:
942 317 228 94 32 32 32
HincII GTy'rAC
Cuts at: 0 917 1524 1677
Size: 917 607 153
Fragments arranged by size:
917 607 153
HpaI GTT'AAC
Cuts at: 0 917 1677
Size: 917 760
KasI G'GCGC_C
Cuts at: 0 172 505 1677
Size: 172 333 1172
Fragments arranged by size:
1172 333 172
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1636 1677
Size: 1636 41
MslI CAynn'nnrTG
Cuts at: 0 907 1112 1475 1583 1677
Size: 907 205 363 108 94
Fragments arranged by size:
907 363 205 108 94
MspA1I CmG'CkG
Cuts at: 0 401 472 1251 1677
Size: 401 71 779 426
Fragments arranged by size:
779 426 401 71
NaeI GCC'GGC
Cuts at: 0 286 1677
Size: 286 1391
NarI GG'CG_CC
Cuts at: 0 173 506 1677
Size: 173 333 1171
Fragments arranged by size:
1171 333 173
NgoMIV G'CCGG_C
Cuts at: 0 284 1677
Size: 284 1393
NspI r_CATG'y
Cuts at: 0 1143 1677
Size: 1143 534
PciI A'CATG_T
Cuts at: 0 1139 1677
Size: 1139 538
PspOMI G'GGCC_C
Cuts at: 0 13 1677
Size: 13 1664
PspXI vC'TCGA_Gb
Cuts at: 0 520 1677
Size: 520 1157
PstI C_TGCA'G
Cuts at: 0 307 1677
Size: 307 1370
PvuII CAG'CTG
Cuts at: 0 1251 1677
Size: 1251 426
SacI G_AGCT'C
Cuts at: 0 228 1344 1677
Size: 228 1116 333
Fragments arranged by size:
1116 333 228
SacII CC_GC'GG
Cuts at: 0 402 473 1677
Size: 402 71 1204
Fragments arranged by size:
1204 402 71
ScaI AGT'ACT
Cuts at: 0 526 1677
Size: 526 1151
SfcI C'TryA_G
Cuts at: 0 303 1677
Size: 303 1374
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 329 350 1677
Size: 329 21 1327
Fragments arranged by size:
1327 329 21
SfoI GGC'GCC
Cuts at: 0 174 507 1677
Size: 174 333 1170
Fragments arranged by size:
1170 333 174
SmaI CCC'GGG
Cuts at: 0 120 254 294 1677
Size: 120 134 40 1383
Fragments arranged by size:
1383 134 120 40
SmlI C'TyrA_G
Cuts at: 0 520 1287 1677
Size: 520 767 390
Fragments arranged by size:
767 520 390
SrfI GCCC'GGGC
Cuts at: 0 294 1677
Size: 294 1383
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 514 753 854 1271 1677
Size: 514 239 101 417 406
Fragments arranged by size:
514 417 406 239 101
TatI w'GTAC_w
Cuts at: 0 524 1312 1571 1677
Size: 524 788 259 106
Fragments arranged by size:
788 524 259 106
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1331 1363 1677
Size: 1331 32 314
Fragments arranged by size:
1331 314 32
XhoI C'TCGA_G
Cuts at: 0 520 1677
Size: 520 1157
XmaI C'CCGG_G
Cuts at: 0 118 252 292 1677
Size: 118 134 40 1385
Fragments arranged by size:
1385 134 118 40
XmnI GAAnn'nnTTC
Cuts at: 0 1041 1458 1677
Size: 1041 417 219
Fragments arranged by size:
1041 417 219
Enzymes that do cut:
AccI AcuI AflIII ApaI ApoI AvaI BanI BanII
BbeI BbsI BbvCI BglI Bme1580I BmgBI BpmI Bpu10I
BpuEI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrFI BssSI BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
HaeII Hin4I HincII HpaI KasI MmeI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PspOMI PspXI PstI
PvuII SacI SacII ScaI SfcI SfiI SfoI SmaI
SmlI SrfI TaqII TatI TstI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI AlwNI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BcgI BciVI BclI BfrBI BglII
BlpI BmrI BmtI BplI BsaI BsaAI BsaBI BsiWI
BsmI BsmBI BsrBI BsrDI BsrGI BssHII BstAPI BstBI
BstEII ClaI CspCI DraI DraIII DrdI EcoNI FseI
FspI FspAI HindIII KpnI MfeI MluI MscI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI
PvuI RsrII SalI SanDI SapI SbfI SexAI SgfI
SgrAI SnaBI SpeI SphI SspI StuI StyI SwaI
Tth111I XbaI XcmI ZraI