Restriction map for
pFN21AE3238
(Linear) (Six-base) MAPSORT of: KIEE3238.seq Check: 7909 from: 1 to: 1596
KIEE3238.orf
With 182 enzymes: SgfI *
July 3, 2012 17:30 ..
AatII G_ACGT'C
Cuts at: 0 917 1596
Size: 917 679
AccI GT'mk_AC
Cuts at: 0 911 1596
Size: 911 685
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 909 1454 1596
Size: 909 545 142
Fragments arranged by size:
909 545 142
AflII C'TTAA_G
Cuts at: 0 646 1069 1596
Size: 646 423 527
Fragments arranged by size:
646 527 423
AleI CACnn'nnGTG
Cuts at: 0 204 1596
Size: 204 1392
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1239 1596
Size: 1239 357
ApoI r'AATT_y
Cuts at: 0 271 402 1321 1596
Size: 271 131 919 275
Fragments arranged by size:
919 275 271 131
AseI AT'TA_AT
Cuts at: 0 422 1596
Size: 422 1174
AvaI C'yCGr_G
Cuts at: 0 223 1596
Size: 223 1373
BanI G'GyrC_C
Cuts at: 0 60 1239 1254 1596
Size: 60 1179 15 342
Fragments arranged by size:
1179 342 60 15
BanII G_rGCy'C
Cuts at: 0 741 1596
Size: 741 855
BbeI G_GCGC'C
Cuts at: 0 64 1596
Size: 64 1532
BbsI GAAGACnn'nnnn_
Cuts at: 0 440 1085 1596
Size: 440 645 511
Fragments arranged by size:
645 511 440
BclI T'GATC_A
Cuts at: 0 1167 1596
Size: 1167 429
BlpI GC'TnA_GC
Cuts at: 0 187 1596
Size: 187 1409
Bme1580I G_kGCm'C
Cuts at: 0 36 632 1140 1596
Size: 36 596 508 456
Fragments arranged by size:
596 508 456 36
BmgBI CAC'GTC
Cuts at: 0 180 1596
Size: 180 1416
BmtI G_CTAG'C
Cuts at: 0 710 1596
Size: 710 886
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 27 58 646 1596
Size: 27 31 588 950
Fragments arranged by size:
950 588 31 27
BsaHI Gr'CG_yC
Cuts at: 0 61 914 1596
Size: 61 853 682
Fragments arranged by size:
853 682 61
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 156 186 1596
Size: 156 30 1410
Fragments arranged by size:
1410 156 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 24 1462 1465 1468 1596
Size: 24 1438 3 3 128
Fragments arranged by size:
1438 128 24 3 3
BseYI C'CCAG_C
Cuts at: 0 751 1596
Size: 751 845
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 903 1596
Size: 903 693
BsiEI CG_ry'CG
Cuts at: 0 48 128 1596
Size: 48 80 1468
Fragments arranged by size:
1468 80 48
BsiHKAI G_wGCw'C
Cuts at: 0 201 741 792 1596
Size: 201 540 51 804
Fragments arranged by size:
804 540 201 51
BsmI GAATG_Cn'
Cuts at: 0 753 1596
Size: 753 843
Bsp1286I G_dGCh'C
Cuts at: 0 36 201 632 741 792 1140 1596
Size: 36 165 431 109 51 348 456
Fragments arranged by size:
456 431 348 165 109 51 36
BsrBI CCG'CTC
Cuts at: 0 132 1596
Size: 132 1464
BsrDI GCAATG_nn'
Cuts at: 0 877 1596
Size: 877 719
BsrGI T'GTAC_A
Cuts at: 0 900 1596
Size: 900 696
BstXI CCAn_nnnn'nTGG
Cuts at: 0 834 1596
Size: 834 762
BstYI r'GATC_y
Cuts at: 0 700 820 1144 1596
Size: 700 120 324 452
Fragments arranged by size:
700 452 324 120
BtsI GCAGTG_nn'
Cuts at: 0 199 640 1596
Size: 199 441 956
Fragments arranged by size:
956 441 199
ClaI AT'CG_AT
Cuts at: 0 1011 1596
Size: 1011 585
DrdI GACnn_nn'nnGTC
Cuts at: 0 1556 1596
Size: 1556 40
EaeI y'GGCC_r
Cuts at: 0 45 125 1315 1596
Size: 45 80 1190 281
Fragments arranged by size:
1190 281 80 45
EagI C'GGCC_G
Cuts at: 0 45 125 1596
Size: 45 80 1471
Fragments arranged by size:
1471 80 45
EarI CTCTTCn'nnn_
Cuts at: 0 50 370 851 1596
Size: 50 320 481 745
Fragments arranged by size:
745 481 320 50
EcoICRI GAG'CTC
Cuts at: 0 739 1596
Size: 739 857
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 27 58 646 909 1454 1596
Size: 27 31 588 263 545 142
Fragments arranged by size:
588 545 263 142 31 27
EcoNI CCTnn'n_nnAGG
Cuts at: 0 9 1596
Size: 9 1587
EcoRI G'AATT_C
Cuts at: 0 402 1596
Size: 402 1194
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 270 362 1596
Size: 270 92 1234
Fragments arranged by size:
1234 270 92
HaeII r_GCGC'y
Cuts at: 0 64 94 231 1596
Size: 64 30 137 1365
Fragments arranged by size:
1365 137 64 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 280 312 1173 1205 1596
Size: 280 32 861 32 391
Fragments arranged by size:
861 391 280 32 32
HincII GTy'rAC
Cuts at: 0 98 912 1596
Size: 98 814 684
Fragments arranged by size:
814 684 98
HindIII A'AGCT_T
Cuts at: 0 583 940 1596
Size: 583 357 656
Fragments arranged by size:
656 583 357
HpaI GTT'AAC
Cuts at: 0 98 1596
Size: 98 1498
KasI G'GCGC_C
Cuts at: 0 60 1596
Size: 60 1536
MslI CAynn'nnrTG
Cuts at: 0 204 889 1596
Size: 204 685 707
Fragments arranged by size:
707 685 204
MspA1I CmG'CkG
Cuts at: 0 79 906 934 1596
Size: 79 827 28 662
Fragments arranged by size:
827 662 79 28
NarI GG'CG_CC
Cuts at: 0 61 1596
Size: 61 1535
NdeI CA'TA_TG
Cuts at: 0 448 1596
Size: 448 1148
NheI G'CTAG_C
Cuts at: 0 706 1596
Size: 706 890
NspI r_CATG'y
Cuts at: 0 901 1596
Size: 901 695
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 978 1010 1596
Size: 978 32 586
Fragments arranged by size:
978 586 32
PshAI GACnn'nnGTC
Cuts at: 0 498 781 1596
Size: 498 283 815
Fragments arranged by size:
815 498 283
PsiI TTA'TAA
Cuts at: 0 889 1294 1327 1596
Size: 889 405 33 269
Fragments arranged by size:
889 405 269 33
PvuII CAG'CTG
Cuts at: 0 906 1596
Size: 906 690
SacI G_AGCT'C
Cuts at: 0 741 1596
Size: 741 855
SalI G'TCGA_C
Cuts at: 0 910 1596
Size: 910 686
SfoI GGC'GCC
Cuts at: 0 62 1596
Size: 62 1534
SmlI C'TyrA_G
Cuts at: 0 646 1069 1596
Size: 646 423 527
Fragments arranged by size:
646 527 423
SspI AAT'ATT
Cuts at: 0 473 1596
Size: 473 1123
StyI C'CwwG_G
Cuts at: 0 1417 1486 1596
Size: 1417 69 110
Fragments arranged by size:
1417 110 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 510 782 1596
Size: 510 272 814
Fragments arranged by size:
814 510 272
TatI w'GTAC_w
Cuts at: 0 900 1596
Size: 900 696
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 670 1596
Size: 670 926
XmnI GAAnn'nnTTC
Cuts at: 0 1308 1586 1596
Size: 1308 278 10
Fragments arranged by size:
1308 278 10
ZraI GAC'GTC
Cuts at: 0 915 1596
Size: 915 681
Enzymes that do cut:
AatII AccI AcuI AflII AleI AlfI ApoI AseI
AvaI BanI BanII BbeI BbsI BclI BlpI Bme1580I
BmgBI BmtI BpmI BsaHI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BsrBI BsrDI BsrGI BstXI
BstYI BtsI ClaI DrdI EaeI EagI EarI EcoICRI
Eco57MI EcoNI EcoRI FalI HaeII Hin4I HincII HindIII
HpaI KasI MslI MspA1I NarI NdeI NheI NspI
PpiI PshAI PsiI PvuII SacI SalI SfoI SmlI
SspI StyI TaqII TatI XcmI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflIII AgeI AhdI AloI
AlwNI ApaI ApaLI AscI AsiSI AvrII BaeI BamHI
BbvCI BcgI BciVI BfrBI BglI BglII BmrI BplI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BsiWI BsmBI
BspEI BspHI BspMI BsrFI BssHII BssSI BstAPI BstBI
BstEII BstZ17I Bsu36I BtgI BtgZI CspCI DraI DraIII
EciI EcoO109I EcoRV FseI FspI FspAI KpnI MfeI
MluI MmeI MscI NaeI NcoI NgoMIV NotI NruI
NsiI PacI PasI PciI PflMI PfoI PmeI PmlI
PpuMI PspOMI PspXI PsrI PstI PvuI RsrII SacII
SanDI SapI SbfI ScaI SexAI SfcI SfiI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI
TstI Tth111I XbaI XhoI XmaI