(Linear) (Six-base) MAPSORT of: KIEE3237.seq  Check: 2233  from: 1  to: 1656



KIEE3237.orf

 With 182 enzymes: SgfI * 

                        July 3, 2012 17:30  ..

AatII G_ACGT'C

Cuts at:      0    1557    1656 
   Size:       1557      99
 
AclI AA'CG_TT

Cuts at:      0     825    1656 
   Size:        825     831
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      33    1442    1656 
   Size:         33    1409     214

  Fragments arranged by size: 

                1409     214      33
 
ApoI r'AATT_y

Cuts at:      0     399     481     514     691     997    1183    1243    1381 
   Size:        399      82      33     177     306     186      60     138

Cuts at:   1381    1656 
   Size:        275

  Fragments arranged by size: 

                 399     306     275     186     177     138      82      60
                  33
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     884    1139    1656 
   Size:        884     255     517

  Fragments arranged by size: 

                 884     517     255
 
BfrBI ATG'CAT

Cuts at:      0     803    1656 
   Size:        803     853
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1130    1475    1656 
   Size:       1130     345     181

  Fragments arranged by size: 

                1130     345     181
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1106    1656 
   Size:       1106     550
 
BsaBI GATnn'nnATC

Cuts at:      0     542    1656 
   Size:        542    1114
 
BsaHI Gr'CG_yC

Cuts at:      0    1554    1656 
   Size:       1554     102
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1473    1503    1656 
   Size:       1473      30     153

  Fragments arranged by size: 

                1473     153      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1267    1322    1656 
   Size:       1267      55     334

  Fragments arranged by size: 

                1267     334      55
 
BsmI GAATG_Cn'

Cuts at:      0    1348    1656 
   Size:       1348     308
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     469    1656 
   Size:        469    1187
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1206    1656 
   Size:       1206     450
 
BstBI TT'CG_AA

Cuts at:      0     396    1656 
   Size:        396    1260
 
BstYI r'GATC_y

Cuts at:      0     609     991    1656 
   Size:        609     382     665

  Fragments arranged by size: 

                 665     609     382
 
BtgI C'CryG_G

Cuts at:      0      62    1656 
   Size:         62    1594
 
BtsI GCAGTG_nn'

Cuts at:      0     560    1470    1656 
   Size:        560     910     186

  Fragments arranged by size: 

                 910     560     186
 
DraIII CAC_nnn'GTG

Cuts at:      0     441    1656 
   Size:        441    1215
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      52    1656 
   Size:         52    1604
 
EaeI y'GGCC_r

Cuts at:      0      17    1656 
   Size:         17    1639
 
EarI CTCTTCn'nnn_

Cuts at:      0    1142    1656 
   Size:       1142     514
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      33    1130    1442    1475    1656 
   Size:         33    1097     312      33     181

  Fragments arranged by size: 

                1097     312     181      33      33
 
EcoRI G'AATT_C

Cuts at:      0     997    1183    1381    1656 
   Size:        997     186     198     275

  Fragments arranged by size: 

                 997     275     198     186
 
EcoRV GAT'ATC

Cuts at:      0     867    1656 
   Size:        867     789
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     901    1656 
   Size:        901     755
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     601     633    1126    1158    1656 
   Size:        601      32     493      32     498

  Fragments arranged by size: 

                 601     498     493      32      32
 
HincII GTy'rAC

Cuts at:      0     224    1526    1656 
   Size:        224    1302     130

  Fragments arranged by size: 

                1302     224     130
 
HindIII A'AGCT_T

Cuts at:      0     367    1193    1656 
   Size:        367     826     463

  Fragments arranged by size: 

                 826     463     367
 
MfeI C'AATT_G

Cuts at:      0     533    1656 
   Size:        533    1123
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     270     877    1656 
   Size:        270     607     779

  Fragments arranged by size: 

                 779     607     270
 
MscI TGG'CCA

Cuts at:      0      19    1656 
   Size:         19    1637
 
MslI CAynn'nnrTG

Cuts at:      0     767    1656 
   Size:        767     889
 
MspA1I CmG'CkG

Cuts at:      0      64    1546    1656 
   Size:         64    1482     110

  Fragments arranged by size: 

                1482     110      64
 
NsiI A_TGCA'T

Cuts at:      0     805    1656 
   Size:        805     851
 
NspI r_CATG'y

Cuts at:      0    1611    1656 
   Size:       1611      45
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1646    1656 
   Size:       1646      10
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1425    1457    1656 
   Size:       1425      32     199

  Fragments arranged by size: 

                1425     199      32
 
PvuII CAG'CTG

Cuts at:      0    1546    1656 
   Size:       1546     110
 
SacII CC_GC'GG

Cuts at:      0      65    1656 
   Size:         65    1591
 
SfcI C'TryA_G

Cuts at:      0     119    1656 
   Size:        119    1537
 
SspI AAT'ATT

Cuts at:      0     975    1656 
   Size:        975     681
 
StuI AGG'CCT

Cuts at:      0    1389    1656 
   Size:       1389     267
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     292    1656 
   Size:        292    1364
 
XbaI T'CTAG_A

Cuts at:      0    1074    1656 
   Size:       1074     582
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     304    1656 
   Size:        304    1352
 
XmnI GAAnn'nnTTC

Cuts at:      0      95     826     944    1656 
   Size:         95     731     118     712

  Fragments arranged by size: 

                 731     712     118      95
 
ZraI GAC'GTC

Cuts at:      0    1555    1656 
   Size:       1555     101
 

 Enzymes that do cut:

    AatII     AclI     AcuI     ApoI     BbsI    BfrBI     BpmI     BsaI
    BsaBI    BsaHI    BsaXI    BseRI     BsmI    BsmBI    BspMI    BstBI
    BstYI     BtgI     BtsI   DraIII     DrdI     EaeI     EarI  Eco57MI
    EcoRI    EcoRV     FalI    Hin4I   HincII  HindIII     MfeI     MmeI
     MscI     MslI   MspA1I     NsiI     NspI    PflMI     PpiI    PvuII
    SacII     SfcI     SspI     StuI    TaqII     XbaI     XcmI     XmnI
     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
    BbvCI     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI    BsaAI    BsaWI
    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI Bsp1286I    BspEI    BspHI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI   BstEII
    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI     EagI
     EciI  EcoICRI    EcoNI EcoO109I     FseI     FspI    FspAI    HaeII
     HpaI     KasI     KpnI     MluI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     PacI     PasI     PciI     PfoI
     PmeI     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    RsrII     SacI     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     StyI     SwaI     TatI     TstI
  Tth111I     XhoI     XmaI