Restriction map for
pFN21AE3234
(Linear) (Six-base) MAPSORT of: KIEE3234.seq Check: 6048 from: 1 to: 1638
KIEE3234.orf
With 182 enzymes: SgfI *
December 27, 2011 16:34 ..
AatII G_ACGT'C
Cuts at: 0 1466 1638
Size: 1466 172
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 886 1279 1638
Size: 886 393 359
Fragments arranged by size:
886 393 359
AlwNI CAG_nnn'CTG
Cuts at: 0 135 1376 1573 1638
Size: 135 1241 197 65
Fragments arranged by size:
1241 197 135 65
ApaLI G'TGCA_C
Cuts at: 0 1046 1638
Size: 1046 592
ApoI r'AATT_y
Cuts at: 0 192 313 622 1638
Size: 192 121 309 1016
Fragments arranged by size:
1016 309 192 121
AvaI C'yCGr_G
Cuts at: 0 302 461 1638
Size: 302 159 1177
Fragments arranged by size:
1177 302 159
BamHI G'GATC_C
Cuts at: 0 28 1638
Size: 28 1610
BanI G'GyrC_C
Cuts at: 0 1472 1638
Size: 1472 166
BbsI GAAGACnn'nnnn_
Cuts at: 0 795 1638
Size: 795 843
BglI GCCn_nnn'nGGC
Cuts at: 0 608 1172 1638
Size: 608 564 466
Fragments arranged by size:
608 564 466
Bme1580I G_kGCm'C
Cuts at: 0 1050 1238 1607 1638
Size: 1050 188 369 31
Fragments arranged by size:
1050 369 188 31
BmrI ACTGGGnnnn_n'
Cuts at: 0 1221 1594 1638
Size: 1221 373 44
Fragments arranged by size:
1221 373 44
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 675 1638
Size: 675 963
Bpu10I CC'TnA_GC
Cuts at: 0 364 1638
Size: 364 1274
BsaI GGTCTCn'nnnn_
Cuts at: 0 35 1638
Size: 35 1603
BsaBI GATnn'nnATC
Cuts at: 0 119 1638
Size: 119 1519
BsaHI Gr'CG_yC
Cuts at: 0 1463 1638
Size: 1463 175
BsaWI w'CCGG_w
Cuts at: 0 37 1638
Size: 37 1601
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 136 194 810 1638
Size: 136 58 616 828
Fragments arranged by size:
828 616 136 58
BseYI C'CCAG_C
Cuts at: 0 357 1638
Size: 357 1281
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 44 193 206 1294 1638
Size: 44 149 13 1088 344
Fragments arranged by size:
1088 344 149 44 13
BsiHKAI G_wGCw'C
Cuts at: 0 1050 1638
Size: 1050 588
BsmI GAATG_Cn'
Cuts at: 0 936 1638
Size: 936 702
Bsp1286I G_dGCh'C
Cuts at: 0 1050 1238 1607 1638
Size: 1050 188 369 31
Fragments arranged by size:
1050 369 188 31
BspEI T'CCGG_A
Cuts at: 0 37 1638
Size: 37 1601
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1526 1638
Size: 1526 112
BsrDI GCAATG_nn'
Cuts at: 0 809 1638
Size: 809 829
BsrFI r'CCGG_y
Cuts at: 0 890 1328 1638
Size: 890 438 310
Fragments arranged by size:
890 438 310
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1233 1638
Size: 1233 405
BstXI CCAn_nnnn'nTGG
Cuts at: 0 492 1041 1638
Size: 492 549 597
Fragments arranged by size:
597 549 492
BstYI r'GATC_y
Cuts at: 0 28 120 771 1638
Size: 28 92 651 867
Fragments arranged by size:
867 651 92 28
BtgI C'CryG_G
Cuts at: 0 1299 1638
Size: 1299 339
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 659 1638
Size: 659 979
BtsI GCAGTG_nn'
Cuts at: 0 27 761 941 1638
Size: 27 734 180 697
Fragments arranged by size:
734 697 180 27
ClaI AT'CG_AT
Cuts at: 0 267 1638
Size: 267 1371
DraIII CAC_nnn'GTG
Cuts at: 0 1305 1425 1638
Size: 1305 120 213
Fragments arranged by size:
1305 213 120
EaeI y'GGCC_r
Cuts at: 0 344 609 1353 1416 1638
Size: 344 265 744 63 222
Fragments arranged by size:
744 344 265 222 63
EarI CTCTTCn'nnn_
Cuts at: 0 60 89 98 224 1478 1638
Size: 60 29 9 126 1254 160
Fragments arranged by size:
1254 160 126 60 29 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1266 1638
Size: 1266 372
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 675 886 1279 1638
Size: 675 211 393 359
Fragments arranged by size:
675 393 359 211
EcoO109I rG'GnC_Cy
Cuts at: 0 210 420 1638
Size: 210 210 1218
Fragments arranged by size:
1218 210 210
EcoRI G'AATT_C
Cuts at: 0 313 1638
Size: 313 1325
EcoRV GAT'ATC
Cuts at: 0 470 1149 1638
Size: 470 679 489
Fragments arranged by size:
679 489 470
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 405 1638
Size: 405 1233
HaeII r_GCGC'y
Cuts at: 0 1592 1638
Size: 1592 46
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 496 528 940 972 1638
Size: 496 32 412 32 666
Fragments arranged by size:
666 496 412 32 32
HindIII A'AGCT_T
Cuts at: 0 438 1181 1638
Size: 438 743 457
Fragments arranged by size:
743 457 438
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 612 1139 1176 1638
Size: 64 548 527 37 462
Fragments arranged by size:
548 527 462 64 37
MscI TGG'CCA
Cuts at: 0 346 1355 1418 1638
Size: 346 1009 63 220
Fragments arranged by size:
1009 346 220 63
MslI CAynn'nnrTG
Cuts at: 0 920 1064 1638
Size: 920 144 574
Fragments arranged by size:
920 574 144
MspA1I CmG'CkG
Cuts at: 0 357 1325 1638
Size: 357 968 313
Fragments arranged by size:
968 357 313
NspI r_CATG'y
Cuts at: 0 1566 1638
Size: 1566 72
PasI CC'CwG_GG
Cuts at: 0 1267 1638
Size: 1267 371
PflMI CCAn_nnn'nTGG
Cuts at: 0 135 1638
Size: 135 1503
PfoI T'CCnGG_A
Cuts at: 0 1553 1638
Size: 1553 85
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 407 439 1638
Size: 407 32 1199
Fragments arranged by size:
1199 407 32
PvuII CAG'CTG
Cuts at: 0 357 1638
Size: 357 1281
ScaI AGT'ACT
Cuts at: 0 839 1549 1638
Size: 839 710 89
Fragments arranged by size:
839 710 89
SfcI C'TryA_G
Cuts at: 0 537 1368 1638
Size: 537 831 270
Fragments arranged by size:
831 537 270
SmaI CCC'GGG
Cuts at: 0 304 1638
Size: 304 1334
SmlI C'TyrA_G
Cuts at: 0 461 1638
Size: 461 1177
SspI AAT'ATT
Cuts at: 0 557 1638
Size: 557 1081
StuI AGG'CCT
Cuts at: 0 1450 1638
Size: 1450 188
StyI C'CwwG_G
Cuts at: 0 447 1638
Size: 447 1191
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 893 1033 1638
Size: 893 140 605
Fragments arranged by size:
893 605 140
TatI w'GTAC_w
Cuts at: 0 837 1547 1638
Size: 837 710 91
Fragments arranged by size:
837 710 91
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1207 1239 1638
Size: 1207 32 399
Fragments arranged by size:
1207 399 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 354 1389 1638
Size: 354 1035 249
Fragments arranged by size:
1035 354 249
XhoI C'TCGA_G
Cuts at: 0 461 1638
Size: 461 1177
XmaI C'CCGG_G
Cuts at: 0 302 1638
Size: 302 1336
XmnI GAAnn'nnTTC
Cuts at: 0 523 694 1638
Size: 523 171 944
Fragments arranged by size:
944 523 171
ZraI GAC'GTC
Cuts at: 0 1464 1638
Size: 1464 174
Enzymes that do cut:
AatII AcuI AlwNI ApaLI ApoI AvaI BamHI BanI
BbsI BglI Bme1580I BmrI BpmI Bpu10I BsaI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspEI BspMI BsrDI BsrFI BstAPI BstXI BstYI BtgI
BtgZI BtsI ClaI DraIII EaeI EarI EciI Eco57MI
EcoO109I EcoRI EcoRV FalI HaeII Hin4I HindIII MmeI
MscI MslI MspA1I NspI PasI PflMI PfoI PpiI
PvuII ScaI SfcI SmaI SmlI SspI StuI StyI
TaqII TatI TstI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI AscI AseI AsiSI
AvrII BaeI BanII BbeI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI BmgBI BmtI BplI BpuEI BsaAI
BsaXI BsiEI BsiWI BsmBI BspHI BsrBI BsrGI BssHII
BssSI BstBI BstEII BstZ17I Bsu36I CspCI DraI DrdI
EagI EcoICRI EcoNI FseI FspI FspAI HincII HpaI
KasI KpnI MfeI MluI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PciI PmeI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI SwaI Tth111I XbaI