Restriction map for
pFN21AE3233
(Linear) (Six-base) MAPSORT of: KIEE3233.seq Check: 4241 from: 1 to: 1629
KIEE3233.orf
With 182 enzymes: SgfI *
December 27, 2011 16:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 837 986 1629
Size: 837 149 643
Fragments arranged by size:
837 643 149
AccI GT'mk_AC
Cuts at: 0 218 1629
Size: 218 1411
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 397 611 1616 1629
Size: 184 213 214 1005 13
Fragments arranged by size:
1005 214 213 184 13
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1210 1242 1629
Size: 1210 32 387
Fragments arranged by size:
1210 387 32
AlwNI CAG_nnn'CTG
Cuts at: 0 531 1629
Size: 531 1098
ApaI G_GGCC'C
Cuts at: 0 555 1247 1629
Size: 555 692 382
Fragments arranged by size:
692 555 382
ApoI r'AATT_y
Cuts at: 0 775 1629
Size: 775 854
AvaI C'yCGr_G
Cuts at: 0 18 740 1050 1629
Size: 18 722 310 579
Fragments arranged by size:
722 579 310 18
BanI G'GyrC_C
Cuts at: 0 233 1629
Size: 233 1396
BanII G_rGCy'C
Cuts at: 0 19 58 555 881 922 1247 1629
Size: 19 39 497 326 41 325 382
Fragments arranged by size:
497 382 326 325 41 39 19
BbeI G_GCGC'C
Cuts at: 0 237 1629
Size: 237 1392
BbsI GAAGACnn'nnnn_
Cuts at: 0 1344 1496 1570 1629
Size: 1344 152 74 59
Fragments arranged by size:
1344 152 74 59
BbvCI CC'TCA_GC
Cuts at: 0 559 1629
Size: 559 1070
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 589 623 1629
Size: 589 34 1006
Fragments arranged by size:
1006 589 34
BfrBI ATG'CAT
Cuts at: 0 7 1629
Size: 7 1622
BglII A'GATC_T
Cuts at: 0 83 1629
Size: 83 1546
BlpI GC'TnA_GC
Cuts at: 0 1148 1317 1629
Size: 1148 169 312
Fragments arranged by size:
1148 312 169
Bme1580I G_kGCm'C
Cuts at: 0 257 555 1117 1247 1629
Size: 257 298 562 130 382
Fragments arranged by size:
562 382 298 257 130
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 897 1629
Size: 897 732
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 72 847 853 1018 1629
Size: 72 775 6 165 611
Fragments arranged by size:
775 611 165 72 6
Bpu10I CC'TnA_GC
Cuts at: 0 559 1629
Size: 559 1070
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 10 991 1500 1629
Size: 10 981 509 129
Fragments arranged by size:
981 509 129 10
BsaI GGTCTCn'nnnn_
Cuts at: 0 28 919 1629
Size: 28 891 710
Fragments arranged by size:
891 710 28
BsaHI Gr'CG_yC
Cuts at: 0 234 1629
Size: 234 1395
BsaWI w'CCGG_w
Cuts at: 0 794 1629
Size: 794 835
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 889 1099 1155 1489 1629
Size: 889 210 56 334 140
Fragments arranged by size:
889 334 210 140 56
BseYI C'CCAG_C
Cuts at: 0 256 287 691 858 1398 1571 1629
Size: 256 31 404 167 540 173 58
Fragments arranged by size:
540 404 256 173 167 58 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1007 1629
Size: 1007 622
BsiHKAI G_wGCw'C
Cuts at: 0 374 915 1629
Size: 374 541 714
Fragments arranged by size:
714 541 374
BsmI GAATG_Cn'
Cuts at: 0 7 1629
Size: 7 1622
Bsp1286I G_dGCh'C
Cuts at: 0 19 58 257 374 555 881 915 922
Size: 19 39 199 117 181 326 34 7
Cuts at: 922 1117 1247 1629
Size: 195 130 382
Fragments arranged by size:
382 326 199 195 181 130 117 39
34 19 7
BspEI T'CCGG_A
Cuts at: 0 794 1629
Size: 794 835
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 837 986 1629
Size: 837 149 643
Fragments arranged by size:
837 643 149
BsrBI CCG'CTC
Cuts at: 0 1002 1629
Size: 1002 627
BsrDI GCAATG_nn'
Cuts at: 0 947 1629
Size: 947 682
BssSI C'ACGA_G
Cuts at: 0 1375 1629
Size: 1375 254
BstAPI GCAn_nnn'nTGC
Cuts at: 0 531 1629
Size: 531 1098
BstYI r'GATC_y
Cuts at: 0 83 1341 1629
Size: 83 1258 288
Fragments arranged by size:
1258 288 83
BstZ17I GTA'TAC
Cuts at: 0 219 1629
Size: 219 1410
Bsu36I CC'TnA_GG
Cuts at: 0 1472 1629
Size: 1472 157
BtsI GCAGTG_nn'
Cuts at: 0 99 134 974 1629
Size: 99 35 840 655
Fragments arranged by size:
840 655 99 35
DraIII CAC_nnn'GTG
Cuts at: 0 322 1629
Size: 322 1307
DrdI GACnn_nn'nnGTC
Cuts at: 0 432 1629
Size: 432 1197
EarI CTCTTCn'nnn_
Cuts at: 0 377 587 1100 1538 1629
Size: 377 210 513 438 91
Fragments arranged by size:
513 438 377 210 91
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 607 1629
Size: 607 1022
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 72 184 397 611 847 853 1018 1616
Size: 72 112 213 214 236 6 165 598
Cuts at: 1616 1629
Size: 13
Fragments arranged by size:
598 236 214 213 165 112 72 13
6
EcoO109I rG'GnC_Cy
Cuts at: 0 552 1244 1629
Size: 552 692 385
Fragments arranged by size:
692 552 385
HaeII r_GCGC'y
Cuts at: 0 237 294 1629
Size: 237 57 1335
Fragments arranged by size:
1335 237 57
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 40 70 72 102 454 486 703 735
Size: 40 30 2 30 352 32 217 32
Cuts at: 735 1629
Size: 894
Fragments arranged by size:
894 352 217 40 32 32 30 30
2
KasI G'GCGC_C
Cuts at: 0 233 1629
Size: 233 1396
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 92 1629
Size: 92 1537
MslI CAynn'nnrTG
Cuts at: 0 817 1629
Size: 817 812
MspA1I CmG'CkG
Cuts at: 0 110 180 1028 1152 1402 1629
Size: 110 70 848 124 250 227
Fragments arranged by size:
848 250 227 124 110 70
NarI GG'CG_CC
Cuts at: 0 234 1629
Size: 234 1395
NsiI A_TGCA'T
Cuts at: 0 9 1629
Size: 9 1620
NspI r_CATG'y
Cuts at: 0 720 1629
Size: 720 909
PfoI T'CCnGG_A
Cuts at: 0 938 1629
Size: 938 691
PspOMI G'GGCC_C
Cuts at: 0 551 1243 1629
Size: 551 692 386
Fragments arranged by size:
692 551 386
PspXI vC'TCGA_Gb
Cuts at: 0 1050 1629
Size: 1050 579
PstI C_TGCA'G
Cuts at: 0 527 685 983 1629
Size: 527 158 298 646
Fragments arranged by size:
646 527 298 158
PvuII CAG'CTG
Cuts at: 0 110 180 1152 1402 1629
Size: 110 70 972 250 227
Fragments arranged by size:
972 250 227 110 70
SapI GCTCTTCn'nnn_
Cuts at: 0 1100 1629
Size: 1100 529
SexAI A'CCwGG_T
Cuts at: 0 206 1629
Size: 206 1423
SfcI C'TryA_G
Cuts at: 0 523 681 979 1629
Size: 523 158 298 650
Fragments arranged by size:
650 523 298 158
SfoI GGC'GCC
Cuts at: 0 235 1629
Size: 235 1394
SmaI CCC'GGG
Cuts at: 0 742 1629
Size: 742 887
SmlI C'TyrA_G
Cuts at: 0 25 1006 1050 1515 1629
Size: 25 981 44 465 114
Fragments arranged by size:
981 465 114 44 25
StuI AGG'CCT
Cuts at: 0 1121 1629
Size: 1121 508
StyI C'CwwG_G
Cuts at: 0 237 1629
Size: 237 1392
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 165 1057 1214 1449 1629
Size: 165 892 157 235 180
Fragments arranged by size:
892 235 180 165 157
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 822 1629
Size: 822 807
XhoI C'TCGA_G
Cuts at: 0 1050 1629
Size: 1050 579
XmaI C'CCGG_G
Cuts at: 0 740 1629
Size: 740 889
XmnI GAAnn'nnTTC
Cuts at: 0 1351 1629
Size: 1351 278
Enzymes that do cut:
AarI AccI AcuI AloI AlwNI ApaI ApoI AvaI
BanI BanII BbeI BbsI BbvCI BcgI BfrBI BglII
BlpI Bme1580I BplI BpmI Bpu10I BpuEI BsaI BsaHI
BsaWI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI
BspMI BsrBI BsrDI BssSI BstAPI BstYI BstZ17I Bsu36I
BtsI DraIII DrdI EarI EciI Eco57MI EcoO109I HaeII
Hin4I KasI MmeI MslI MspA1I NarI NsiI NspI
PfoI PspOMI PspXI PstI PvuII SapI SexAI SfcI
SfoI SmaI SmlI StuI StyI TaqII XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BciVI BclI BglI BmgBI BmrI BmtI BsaAI
BsaBI BsaXI BsiEI BsiWI BsmBI BspHI BsrFI BsrGI
BssHII BstBI BstEII BstXI BtgI BtgZI ClaI CspCI
DraI EaeI EagI EcoICRI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI KpnI MfeI
MluI MscI NaeI NcoI NdeI NgoMIV NheI NotI
NruI PacI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PsrI PvuI RsrII SacI SacII
SalI SanDI SbfI ScaI SfiI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI SwaI TatI TstI Tth111I
XbaI ZraI