Restriction map for
pFN21AE3230
(Linear) (Six-base) MAPSORT of: KIEE3230.seq Check: 9800 from: 1 to: 1608
KIEE3230.orf
With 182 enzymes: SgfI *
March 22, 2012 16:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1435 1608
Size: 1435 173
AatII G_ACGT'C
Cuts at: 0 1167 1608
Size: 1167 441
AccI GT'mk_AC
Cuts at: 0 1535 1608
Size: 1535 73
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 27 358 1608
Size: 27 331 1250
Fragments arranged by size:
1250 331 27
AfeI AGC'GCT
Cuts at: 0 793 1608
Size: 793 815
AleI CACnn'nnGTG
Cuts at: 0 999 1608
Size: 999 609
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1017 1608
Size: 1017 591
AlwNI CAG_nnn'CTG
Cuts at: 0 213 333 398 1608
Size: 213 120 65 1210
Fragments arranged by size:
1210 213 120 65
ApaLI G'TGCA_C
Cuts at: 0 160 710 1608
Size: 160 550 898
Fragments arranged by size:
898 550 160
ApoI r'AATT_y
Cuts at: 0 133 533 575 657 1608
Size: 133 400 42 82 951
Fragments arranged by size:
951 400 133 82 42
AvaI C'yCGr_G
Cuts at: 0 550 1518 1567 1592 1608
Size: 550 968 49 25 16
Fragments arranged by size:
968 550 49 25 16
BanI G'GyrC_C
Cuts at: 0 703 898 1608
Size: 703 195 710
Fragments arranged by size:
710 703 195
BanII G_rGCy'C
Cuts at: 0 324 935 1608
Size: 324 611 673
Fragments arranged by size:
673 611 324
BbsI GAAGACnn'nnnn_
Cuts at: 0 84 1608
Size: 84 1524
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 32 1608
Size: 32 1576
BciVI GTATCCnnnnn_n'
Cuts at: 0 1062 1608
Size: 1062 546
BglI GCCn_nnn'nGGC
Cuts at: 0 328 1402 1608
Size: 328 1074 206
Fragments arranged by size:
1074 328 206
BglII A'GATC_T
Cuts at: 0 123 1608
Size: 123 1485
BlpI GC'TnA_GC
Cuts at: 0 733 1608
Size: 733 875
Bme1580I G_kGCm'C
Cuts at: 0 164 714 901 1463 1608
Size: 164 550 187 562 145
Fragments arranged by size:
562 550 187 164 145
BmrI ACTGGGnnnn_n'
Cuts at: 0 426 842 1548 1608
Size: 426 416 706 60
Fragments arranged by size:
706 426 416 60
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 576 1608
Size: 576 1032
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 173 338 402 466 791 889 1083 1608
Size: 173 165 64 64 325 98 194 525
Fragments arranged by size:
525 325 194 173 165 98 64 64
Bpu10I CC'TnA_GC
Cuts at: 0 467 1608
Size: 467 1141
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 245 1608
Size: 245 1363
BsaHI Gr'CG_yC
Cuts at: 0 1164 1608
Size: 1164 444
BsaWI w'CCGG_w
Cuts at: 0 1602 1608
Size: 1602 6
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 338 368 370 400 1608
Size: 338 30 2 30 1208
Fragments arranged by size:
1208 338 30 30 2
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 739 1334 1608
Size: 739 595 274
Fragments arranged by size:
739 595 274
BseYI C'CCAG_C
Cuts at: 0 35 323 843 980 1608
Size: 35 288 520 137 628
Fragments arranged by size:
628 520 288 137 35
BsiHKAI G_wGCw'C
Cuts at: 0 11 164 468 714 1230 1608
Size: 11 153 304 246 516 378
Fragments arranged by size:
516 378 304 246 153 11
BsmI GAATG_Cn'
Cuts at: 0 1201 1608
Size: 1201 407
Bsp1286I G_dGCh'C
Cuts at: 0 11 164 324 468 714 901 935 1230
Size: 11 153 160 144 246 187 34 295
Cuts at: 1230 1463 1608
Size: 233 145
Fragments arranged by size:
295 246 233 187 160 153 145 144
34 11
BspEI T'CCGG_A
Cuts at: 0 1602 1608
Size: 1602 6
BspHI T'CATG_A
Cuts at: 0 1022 1608
Size: 1022 586
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 45 1435 1608
Size: 45 1390 173
Fragments arranged by size:
1390 173 45
BsrDI GCAATG_nn'
Cuts at: 0 881 1608
Size: 881 727
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1098 1608
Size: 1098 510
BstYI r'GATC_y
Cuts at: 0 123 1034 1608
Size: 123 911 574
Fragments arranged by size:
911 574 123
BtsI GCAGTG_nn'
Cuts at: 0 1081 1391 1608
Size: 1081 310 217
Fragments arranged by size:
1081 310 217
DraI TTT'AAA
Cuts at: 0 1008 1608
Size: 1008 600
DrdI GACnn_nn'nnGTC
Cuts at: 0 886 1608
Size: 886 722
EaeI y'GGCC_r
Cuts at: 0 1250 1310 1608
Size: 1250 60 298
Fragments arranged by size:
1250 298 60
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 1608
Size: 19 1589
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 27 173 338 358 402 466 791 889
Size: 27 146 165 20 44 64 325 98
Cuts at: 889 1083 1608
Size: 194 525
Fragments arranged by size:
525 325 194 165 146 98 64 44
27 20
EcoO109I rG'GnC_Cy
Cuts at: 0 491 1542 1597 1608
Size: 491 1051 55 11
Fragments arranged by size:
1051 491 55 11
EcoRV GAT'ATC
Cuts at: 0 219 1608
Size: 219 1389
HaeII r_GCGC'y
Cuts at: 0 795 1608
Size: 795 813
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 370 402 1019 1036 1051 1068 1608
Size: 370 32 617 17 15 17 540
Fragments arranged by size:
617 540 370 32 17 17 15
HincII GTy'rAC
Cuts at: 0 975 1608
Size: 975 633
HindIII A'AGCT_T
Cuts at: 0 339 730 1608
Size: 339 391 878
Fragments arranged by size:
878 391 339
MscI TGG'CCA
Cuts at: 0 1252 1312 1608
Size: 1252 60 296
Fragments arranged by size:
1252 296 60
MslI CAynn'nnrTG
Cuts at: 0 167 999 1096 1608
Size: 167 832 97 512
Fragments arranged by size:
832 512 167 97
MspA1I CmG'CkG
Cuts at: 0 23 444 479 1608
Size: 23 421 35 1129
Fragments arranged by size:
1129 421 35 23
PasI CC'CwG_GG
Cuts at: 0 1207 1608
Size: 1207 401
PflMI CCAn_nnn'nTGG
Cuts at: 0 136 213 1608
Size: 136 77 1395
Fragments arranged by size:
1395 136 77
PfoI T'CCnGG_A
Cuts at: 0 807 1060 1608
Size: 807 253 548
Fragments arranged by size:
807 548 253
PpuMI rG'GwC_Cy
Cuts at: 0 491 1608
Size: 491 1117
PspXI vC'TCGA_Gb
Cuts at: 0 1567 1608
Size: 1567 41
PstI C_TGCA'G
Cuts at: 0 1088 1608
Size: 1088 520
PvuII CAG'CTG
Cuts at: 0 444 479 1608
Size: 444 35 1129
Fragments arranged by size:
1129 444 35
SanDI GG'GwC_CC
Cuts at: 0 491 1608
Size: 491 1117
ScaI AGT'ACT
Cuts at: 0 194 1608
Size: 194 1414
SfcI C'TryA_G
Cuts at: 0 636 1084 1608
Size: 636 448 524
Fragments arranged by size:
636 524 448
SmaI CCC'GGG
Cuts at: 0 1520 1594 1608
Size: 1520 74 14
Fragments arranged by size:
1520 74 14
SmlI C'TyrA_G
Cuts at: 0 260 1567 1608
Size: 260 1307 41
Fragments arranged by size:
1307 260 41
StuI AGG'CCT
Cuts at: 0 556 1274 1608
Size: 556 718 334
Fragments arranged by size:
718 556 334
StyI C'CwwG_G
Cuts at: 0 612 699 1608
Size: 612 87 909
Fragments arranged by size:
909 612 87
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 448 1608
Size: 448 1160
TatI w'GTAC_w
Cuts at: 0 192 1608
Size: 192 1416
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 338 370 803 835 1442 1474 1608
Size: 338 32 433 32 607 32 134
Fragments arranged by size:
607 433 338 134 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 197 697 942 1608
Size: 197 500 245 666
Fragments arranged by size:
666 500 245 197
XhoI C'TCGA_G
Cuts at: 0 1567 1608
Size: 1567 41
XmaI C'CCGG_G
Cuts at: 0 1518 1592 1608
Size: 1518 74 16
Fragments arranged by size:
1518 74 16
XmnI GAAnn'nnTTC
Cuts at: 0 91 1608
Size: 91 1517
ZraI GAC'GTC
Cuts at: 0 1165 1608
Size: 1165 443
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AleI AlfI AlwNI
ApaLI ApoI AvaI BanI BanII BbsI BcgI BciVI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaHI BsaWI BsaXI BseRI BseYI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrDI BstXI BstYI BtsI
DraI DrdI EaeI EciI Eco57MI EcoO109I EcoRV HaeII
Hin4I HincII HindIII MscI MslI MspA1I PasI PflMI
PfoI PpuMI PspXI PstI PvuII SanDI ScaI SfcI
SmaI SmlI StuI StyI TaqII TatI TstI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AflII AflIII AgeI AhdI AloI ApaI
AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI
BclI BfrBI BmgBI BmtI BsaI BsaAI BsaBI BsgI
BsiEI BsiWI BsmBI BsrBI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI ClaI
CspCI DraIII EagI EarI EcoICRI EcoNI EcoRI FalI
FseI FspI FspAI HpaI KasI KpnI MfeI MluI
MmeI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PciI PmeI PmlI PpiI
PshAI PsiI PspOMI PsrI PvuI RsrII SacI SacII
SalI SapI SbfI SexAI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI