Restriction map for
pFN21AE3223
(Linear) (Six-base) MAPSORT of: KIEE3223.seq Check: 3980 from: 1 to: 1518
KIEE3223.orf
With 182 enzymes: SgfI *
March 22, 2012 16:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 654 771 1518
Size: 654 117 747
Fragments arranged by size:
747 654 117
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 406 1228 1342 1518
Size: 406 822 114 176
Fragments arranged by size:
822 406 176 114
AflIII A'CryG_T
Cuts at: 0 617 1518
Size: 617 901
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 972 1004 1518
Size: 972 32 514
Fragments arranged by size:
972 514 32
AlwNI CAG_nnn'CTG
Cuts at: 0 96 171 414 460 600 832 1338 1452
Size: 96 75 243 46 140 232 506 114
Cuts at: 1452 1518
Size: 66
Fragments arranged by size:
506 243 232 140 114 96 75 66
46
ApaI G_GGCC'C
Cuts at: 0 1012 1518
Size: 1012 506
ApoI r'AATT_y
Cuts at: 0 1303 1518
Size: 1303 215
AvaI C'yCGr_G
Cuts at: 0 763 1518
Size: 763 755
BamHI G'GATC_C
Cuts at: 0 909 1518
Size: 909 609
BanI G'GyrC_C
Cuts at: 0 1487 1518
Size: 1487 31
BanII G_rGCy'C
Cuts at: 0 655 740 1012 1070 1518
Size: 655 85 272 58 448
Fragments arranged by size:
655 448 272 85 58
BbeI G_GCGC'C
Cuts at: 0 1491 1518
Size: 1491 27
BbsI GAAGACnn'nnnn_
Cuts at: 0 1234 1438 1518
Size: 1234 204 80
Fragments arranged by size:
1234 204 80
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 30 64 931 965 1518
Size: 30 34 867 34 553
Fragments arranged by size:
867 553 34 34 30
BglII A'GATC_T
Cuts at: 0 1082 1518
Size: 1082 436
BlpI GC'TnA_GC
Cuts at: 0 590 1518
Size: 590 928
Bme1580I G_kGCm'C
Cuts at: 0 23 1012 1518
Size: 23 989 506
Fragments arranged by size:
989 506 23
BmgBI CAC'GTC
Cuts at: 0 618 1518
Size: 618 900
BmrI ACTGGGnnnn_n'
Cuts at: 0 813 888 942 1518
Size: 813 75 54 576
Fragments arranged by size:
813 576 75 54
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 396 1518
Size: 396 1122
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 935 974 1142 1234 1294 1423 1447 1518
Size: 935 39 168 92 60 129 24 71
Fragments arranged by size:
935 168 129 92 71 60 39 24
Bpu10I CC'TnA_GC
Cuts at: 0 776 1409 1518
Size: 776 633 109
Fragments arranged by size:
776 633 109
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1099 1405 1518
Size: 1099 306 113
Fragments arranged by size:
1099 306 113
BsaI GGTCTCn'nnnn_
Cuts at: 0 1036 1518
Size: 1036 482
BsaAI yAC'GTr
Cuts at: 0 18 1518
Size: 18 1500
BsaHI Gr'CG_yC
Cuts at: 0 628 1488 1518
Size: 628 860 30
Fragments arranged by size:
860 628 30
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 430 460 972 1002 1518
Size: 430 30 512 30 516
Fragments arranged by size:
516 512 430 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 454 457 542 781 1132 1402 1518
Size: 454 3 85 239 351 270 116
Fragments arranged by size:
454 351 270 239 116 85 3
BseYI C'CCAG_C
Cuts at: 0 164 1331 1518
Size: 164 1167 187
Fragments arranged by size:
1167 187 164
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 709 1384 1518
Size: 709 675 134
Fragments arranged by size:
709 675 134
BsiHKAI G_wGCw'C
Cuts at: 0 1070 1518
Size: 1070 448
BsmBI CGTCTCn'nnnn_
Cuts at: 0 932 1518
Size: 932 586
Bsp1286I G_dGCh'C
Cuts at: 0 23 655 740 1012 1070 1518
Size: 23 632 85 272 58 448
Fragments arranged by size:
632 448 272 85 58 23
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 563 654 771 1427 1518
Size: 563 91 117 656 91
Fragments arranged by size:
656 563 117 91 91
BstAPI GCAn_nnn'nTGC
Cuts at: 0 460 669 793 1518
Size: 460 209 124 725
Fragments arranged by size:
725 460 209 124
BstEII G'GTnAC_C
Cuts at: 0 1194 1518
Size: 1194 324
BstYI r'GATC_y
Cuts at: 0 374 909 1082 1218 1518
Size: 374 535 173 136 300
Fragments arranged by size:
535 374 300 173 136
BtgI C'CryG_G
Cuts at: 0 1199 1518
Size: 1199 319
BtsI GCAGTG_nn'
Cuts at: 0 72 198 833 1024 1109 1518
Size: 72 126 635 191 85 409
Fragments arranged by size:
635 409 191 126 85 72
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1268 1303 1518
Size: 1268 35 215
Fragments arranged by size:
1268 215 35
EaeI y'GGCC_r
Cuts at: 0 258 1518
Size: 258 1260
EarI CTCTTCn'nnn_
Cuts at: 0 149 273 1518
Size: 149 124 1245
Fragments arranged by size:
1245 149 124
EcoICRI GAG'CTC
Cuts at: 0 1068 1518
Size: 1068 450
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 406 935 974 1142 1228 1234 1294 1342
Size: 406 529 39 168 86 6 60 48
Cuts at: 1342 1423 1447 1518
Size: 81 24 71
Fragments arranged by size:
529 406 168 86 81 71 60 48
39 24 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 547 1278 1518
Size: 547 731 240
Fragments arranged by size:
731 547 240
EcoO109I rG'GnC_Cy
Cuts at: 0 179 552 1008 1518
Size: 179 373 456 510
Fragments arranged by size:
510 456 373 179
EcoRI G'AATT_C
Cuts at: 0 1303 1518
Size: 1303 215
HaeII r_GCGC'y
Cuts at: 0 1491 1518
Size: 1491 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 288 320 746 778 814 846 1035 1067
Size: 288 32 426 32 36 32 189 32
Cuts at: 1067 1117 1149 1518
Size: 50 32 369
Fragments arranged by size:
426 369 288 189 50 36 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 366 1518
Size: 366 1152
HindIII A'AGCT_T
Cuts at: 0 1231 1518
Size: 1231 287
KasI G'GCGC_C
Cuts at: 0 1487 1518
Size: 1487 31
MslI CAynn'nnrTG
Cuts at: 0 616 1518
Size: 616 902
MspA1I CmG'CkG
Cuts at: 0 168 276 414 567 586 1152 1248 1335
Size: 168 108 138 153 19 566 96 87
Cuts at: 1335 1484 1518
Size: 149 34
Fragments arranged by size:
566 168 153 149 138 108 96 87
34 19
NarI GG'CG_CC
Cuts at: 0 1488 1518
Size: 1488 30
NcoI C'CATG_G
Cuts at: 0 1199 1518
Size: 1199 319
NspI r_CATG'y
Cuts at: 0 144 1518
Size: 144 1374
PasI CC'CwG_GG
Cuts at: 0 600 1518
Size: 600 918
PflMI CCAn_nnn'nTGG
Cuts at: 0 1338 1518
Size: 1338 180
PfoI T'CCnGG_A
Cuts at: 0 134 212 890 912 1518
Size: 134 78 678 22 606
Fragments arranged by size:
678 606 134 78 22
PmlI CAC'GTG
Cuts at: 0 18 1518
Size: 18 1500
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 972 1004 1518
Size: 972 32 514
Fragments arranged by size:
972 514 32
PpuMI rG'GwC_Cy
Cuts at: 0 179 552 1518
Size: 179 373 966
Fragments arranged by size:
966 373 179
PspOMI G'GGCC_C
Cuts at: 0 1008 1518
Size: 1008 510
PspXI vC'TCGA_Gb
Cuts at: 0 763 1518
Size: 763 755
PstI C_TGCA'G
Cuts at: 0 79 419 1172 1448 1518
Size: 79 340 753 276 70
Fragments arranged by size:
753 340 276 79 70
PvuII CAG'CTG
Cuts at: 0 168 414 567 1152 1248 1335 1518
Size: 168 246 153 585 96 87 183
Fragments arranged by size:
585 246 183 168 153 96 87
SacI G_AGCT'C
Cuts at: 0 1070 1518
Size: 1070 448
SbfI CC_TGCA'GG
Cuts at: 0 1172 1518
Size: 1172 346
SfcI C'TryA_G
Cuts at: 0 75 415 1168 1444 1518
Size: 75 340 753 276 74
Fragments arranged by size:
753 340 276 75 74
SfoI GGC'GCC
Cuts at: 0 1489 1518
Size: 1489 29
SmlI C'TyrA_G
Cuts at: 0 763 1078 1384 1518
Size: 763 315 306 134
Fragments arranged by size:
763 315 306 134
SspI AAT'ATT
Cuts at: 0 252 1518
Size: 252 1266
StuI AGG'CCT
Cuts at: 0 238 1518
Size: 238 1280
StyI C'CwwG_G
Cuts at: 0 1199 1279 1436 1518
Size: 1199 80 157 82
Fragments arranged by size:
1199 157 82 80
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1307 1518
Size: 1307 211
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 430 462 1518
Size: 430 32 1056
Fragments arranged by size:
1056 430 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 172 1188 1518
Size: 172 1016 330
Fragments arranged by size:
1016 330 172
XhoI C'TCGA_G
Cuts at: 0 763 1518
Size: 763 755
XmnI GAAnn'nnTTC
Cuts at: 0 1054 1518
Size: 1054 464
Enzymes that do cut:
AarI AcuI AflIII AloI AlwNI ApaI ApoI AvaI
BamHI BanI BanII BbeI BbsI BcgI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI
Bsp1286I BspMI BstAPI BstEII BstYI BtgI BtsI CspCI
EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI HaeII
Hin4I HincII HindIII KasI MslI MspA1I NarI NcoI
NspI PasI PflMI PfoI PmlI PpiI PpuMI PspOMI
PspXI PstI PvuII SacI SbfI SfcI SfoI SmlI
SspI StuI StyI TaqII TstI XcmI XhoI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI ApaLI AscI AseI AsiSI AvrII BaeI
BbvCI BciVI BclI BfrBI BglI BmtI BsaBI BsaWI
BsiEI BsiWI BsmI BspEI BspHI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstBI BstXI BstZ17I Bsu36I BtgZI
ClaI DraI DraIII DrdI EagI EciI EcoRV FalI
FseI FspI FspAI HpaI KpnI MfeI MluI MmeI
MscI NaeI NdeI NgoMIV NheI NotI NruI NsiI
PacI PciI PmeI PshAI PsiI PsrI PvuI RsrII
SacII SalI SanDI SapI ScaI SexAI SfiI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SwaI TatI
Tth111I XbaI XmaI ZraI