Restriction map for
pFN21AE3220
(Linear) (Six-base) MAPSORT of: KIEE3220.seq Check: 5744 from: 1 to: 1488
KIEE3220.orf
With 182 enzymes: SgfI *
July 3, 2012 17:28 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 457 825 875 1031 1488
Size: 457 368 50 156 457
Fragments arranged by size:
457 457 368 156 50
AflIII A'CryG_T
Cuts at: 0 179 893 1488
Size: 179 714 595
Fragments arranged by size:
714 595 179
AlwNI CAG_nnn'CTG
Cuts at: 0 237 848 1488
Size: 237 611 640
Fragments arranged by size:
640 611 237
ApoI r'AATT_y
Cuts at: 0 669 1488
Size: 669 819
BbsI GAAGACnn'nnnn_
Cuts at: 0 602 1488
Size: 602 886
BciVI GTATCCnnnnn_n'
Cuts at: 0 669 1488
Size: 669 819
BfrBI ATG'CAT
Cuts at: 0 1345 1488
Size: 1345 143
BmgBI CAC'GTC
Cuts at: 0 982 1488
Size: 982 506
BmrI ACTGGGnnnn_n'
Cuts at: 0 1140 1488
Size: 1140 348
BmtI G_CTAG'C
Cuts at: 0 589 1488
Size: 589 899
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1167 1488
Size: 1167 321
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 153 268 971 1488
Size: 153 115 703 517
Fragments arranged by size:
703 517 153 115
Bpu10I CC'TnA_GC
Cuts at: 0 803 1437 1488
Size: 803 634 51
Fragments arranged by size:
803 634 51
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 310 1161 1435 1488
Size: 310 851 274 53
Fragments arranged by size:
851 310 274 53
BsaAI yAC'GTr
Cuts at: 0 180 1107 1488
Size: 180 927 381
Fragments arranged by size:
927 381 180
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 968 1488
Size: 968 520
BseYI C'CCAG_C
Cuts at: 0 1062 1488
Size: 1062 426
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 250 1488
Size: 214 36 1238
Fragments arranged by size:
1238 214 36
BsmI GAATG_Cn'
Cuts at: 0 348 1488
Size: 348 1140
BsrBI CCG'CTC
Cuts at: 0 161 210 1488
Size: 161 49 1278
Fragments arranged by size:
1278 161 49
BsrFI r'CCGG_y
Cuts at: 0 1228 1488
Size: 1228 260
BssSI C'ACGA_G
Cuts at: 0 459 1488
Size: 459 1029
BstAPI GCAn_nnn'nTGC
Cuts at: 0 237 1488
Size: 237 1251
BstEII G'GTnAC_C
Cuts at: 0 1039 1488
Size: 1039 449
BtgI C'CryG_G
Cuts at: 0 710 1488
Size: 710 778
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 447 482 1488
Size: 447 35 1006
Fragments arranged by size:
1006 447 35
DraIII CAC_nnn'GTG
Cuts at: 0 275 1488
Size: 275 1213
DrdI GACnn_nn'nnGTC
Cuts at: 0 783 1488
Size: 783 705
EaeI y'GGCC_r
Cuts at: 0 44 1488
Size: 44 1444
EarI CTCTTCn'nnn_
Cuts at: 0 64 168 626 914 1488
Size: 64 104 458 288 574
Fragments arranged by size:
574 458 288 104 64
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 153 268 457 825 875 971 1031 1488
Size: 153 115 189 368 50 96 60 457
Fragments arranged by size:
457 368 189 153 115 96 60 50
EcoO109I rG'GnC_Cy
Cuts at: 0 116 807 1488
Size: 116 691 681
Fragments arranged by size:
691 681 116
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 18 50 1488
Size: 18 32 1438
Fragments arranged by size:
1438 32 18
HincII GTy'rAC
Cuts at: 0 425 899 1488
Size: 425 474 589
Fragments arranged by size:
589 474 425
HindIII A'AGCT_T
Cuts at: 0 286 1488
Size: 286 1202
HpaI GTT'AAC
Cuts at: 0 425 899 1488
Size: 425 474 589
Fragments arranged by size:
589 474 425
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 636 1382 1488
Size: 636 746 106
Fragments arranged by size:
746 636 106
MslI CAynn'nnrTG
Cuts at: 0 1462 1488
Size: 1462 26
MspA1I CmG'CkG
Cuts at: 0 406 1488
Size: 406 1082
NaeI GCC'GGC
Cuts at: 0 1230 1488
Size: 1230 258
NgoMIV G'CCGG_C
Cuts at: 0 1228 1488
Size: 1228 260
NheI G'CTAG_C
Cuts at: 0 585 1488
Size: 585 903
NsiI A_TGCA'T
Cuts at: 0 1347 1488
Size: 1347 141
NspI r_CATG'y
Cuts at: 0 1349 1488
Size: 1349 139
PfoI T'CCnGG_A
Cuts at: 0 302 1488
Size: 302 1186
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 461 476 493 508 1488
Size: 461 15 17 15 980
Fragments arranged by size:
980 461 17 15 15
PpuMI rG'GwC_Cy
Cuts at: 0 116 807 1488
Size: 116 691 681
Fragments arranged by size:
691 681 116
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1357 1389 1488
Size: 1357 32 99
Fragments arranged by size:
1357 99 32
PstI C_TGCA'G
Cuts at: 0 124 1488
Size: 124 1364
SapI GCTCTTCn'nnn_
Cuts at: 0 64 168 1488
Size: 64 104 1320
Fragments arranged by size:
1320 104 64
SfcI C'TryA_G
Cuts at: 0 120 616 646 914 1488
Size: 120 496 30 268 574
Fragments arranged by size:
574 496 268 120 30
SmlI C'TyrA_G
Cuts at: 0 289 1176 1450 1488
Size: 289 887 274 38
Fragments arranged by size:
887 289 274 38
SspI AAT'ATT
Cuts at: 0 1057 1488
Size: 1057 431
StuI AGG'CCT
Cuts at: 0 931 1488
Size: 931 557
TatI w'GTAC_w
Cuts at: 0 348 1373 1488
Size: 348 1025 115
Fragments arranged by size:
1025 348 115
XmnI GAAnn'nnTTC
Cuts at: 0 565 1488
Size: 565 923
Enzymes that do cut:
AcuI AflIII AlwNI ApoI BbsI BciVI BfrBI BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaAI BseRI
BseYI BsgI BsmI BsrBI BsrFI BssSI BstAPI BstEII
BtgI CspCI DraIII DrdI EaeI EarI Eco57MI EcoO109I
Hin4I HincII HindIII HpaI MmeI MslI MspA1I NaeI
NgoMIV NheI NsiI NspI PfoI PpiI PpuMI PsrI
PstI SapI SfcI SmlI SspI StuI TatI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI
BbvCI BcgI BclI BglI BglII BlpI Bme1580I BsaI
BsaBI BsaHI BsaWI BsaXI BsiEI BsiHKAI BsiWI BsmBI
Bsp1286I BspEI BspHI BspMI BsrDI BsrGI BssHII BstBI
BstXI BstYI BstZ17I Bsu36I BtgZI BtsI ClaI DraI
EagI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HaeII KasI KpnI MfeI MluI MscI
NarI NcoI NdeI NotI NruI PacI PasI PciI
PflMI PmeI PmlI PshAI PsiI PspOMI PspXI PvuI
PvuII RsrII SacI SacII SalI SanDI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI StyI SwaI TaqII TstI Tth111I XbaI
XcmI XhoI XmaI ZraI