Restriction map for
pFN21AE3219
(Linear) (Six-base) MAPSORT of: KIEE3219.seq Check: 6819 from: 1 to: 1485
KIEE3219.orf
With 182 enzymes: SgfI *
July 3, 2012 17:28 ..
AatII G_ACGT'C
Cuts at: 0 1244 1485
Size: 1244 241
AclI AA'CG_TT
Cuts at: 0 1364 1485
Size: 1364 121
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 464 694 1485
Size: 464 230 791
Fragments arranged by size:
791 464 230
AfeI AGC'GCT
Cuts at: 0 1171 1485
Size: 1171 314
AflIII A'CryG_T
Cuts at: 0 479 1073 1232 1485
Size: 479 594 159 253
Fragments arranged by size:
594 479 253 159
AleI CACnn'nnGTG
Cuts at: 0 949 1485
Size: 949 536
AlwNI CAG_nnn'CTG
Cuts at: 0 183 249 555 1009 1485
Size: 183 66 306 454 476
Fragments arranged by size:
476 454 306 183 66
ApaI G_GGCC'C
Cuts at: 0 1018 1485
Size: 1018 467
ApoI r'AATT_y
Cuts at: 0 135 1485
Size: 135 1350
BamHI G'GATC_C
Cuts at: 0 1292 1485
Size: 1292 193
BanII G_rGCy'C
Cuts at: 0 299 1018 1485
Size: 299 719 467
Fragments arranged by size:
719 467 299
BbsI GAAGACnn'nnnn_
Cuts at: 0 375 1485
Size: 375 1110
BbvCI CC'TCA_GC
Cuts at: 0 217 719 1485
Size: 217 502 766
Fragments arranged by size:
766 502 217
BfrBI ATG'CAT
Cuts at: 0 516 1485
Size: 516 969
BglI GCCn_nnn'nGGC
Cuts at: 0 1013 1485
Size: 1013 472
BlpI GC'TnA_GC
Cuts at: 0 1108 1485
Size: 1108 377
Bme1580I G_kGCm'C
Cuts at: 0 1018 1485
Size: 1018 467
BmgBI CAC'GTC
Cuts at: 0 480 1074 1485
Size: 480 594 411
Fragments arranged by size:
594 480 411
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 283 364 1485
Size: 283 81 1121
Fragments arranged by size:
1121 283 81
Bpu10I CC'TnA_GC
Cuts at: 0 217 719 1485
Size: 217 502 766
Fragments arranged by size:
766 502 217
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 917 1485
Size: 917 568
BsaI GGTCTCn'nnnn_
Cuts at: 0 639 1485
Size: 639 846
BsaHI Gr'CG_yC
Cuts at: 0 79 1241 1485
Size: 79 1162 244
Fragments arranged by size:
1162 244 79
BsaWI w'CCGG_w
Cuts at: 0 1384 1485
Size: 1384 101
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1297 1327 1485
Size: 1297 30 158
Fragments arranged by size:
1297 158 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 175 489 875 900 1485
Size: 175 314 386 25 585
Fragments arranged by size:
585 386 314 175 25
BseYI C'CCAG_C
Cuts at: 0 1103 1434 1485
Size: 1103 331 51
Fragments arranged by size:
1103 331 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 346 554 1485
Size: 346 208 931
Fragments arranged by size:
931 346 208
BsiHKAI G_wGCw'C
Cuts at: 0 299 1485
Size: 299 1186
BsmBI CGTCTCn'nnnn_
Cuts at: 0 470 1485
Size: 470 1015
Bsp1286I G_dGCh'C
Cuts at: 0 299 1018 1485
Size: 299 719 467
Fragments arranged by size:
719 467 299
BspEI T'CCGG_A
Cuts at: 0 1384 1485
Size: 1384 101
BspHI T'CATG_A
Cuts at: 0 275 1485
Size: 275 1210
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 359 1485
Size: 359 1126
BssSI C'ACGA_G
Cuts at: 0 1264 1485
Size: 1264 221
BstAPI GCAn_nnn'nTGC
Cuts at: 0 555 1485
Size: 555 930
BstEII G'GTnAC_C
Cuts at: 0 628 1485
Size: 628 857
BstXI CCAn_nnnn'nTGG
Cuts at: 0 951 1485
Size: 951 534
BstYI r'GATC_y
Cuts at: 0 245 551 669 1292 1485
Size: 245 306 118 623 193
Fragments arranged by size:
623 306 245 193 118
BtgI C'CryG_G
Cuts at: 0 67 1485
Size: 67 1418
DraI TTT'AAA
Cuts at: 0 1317 1485
Size: 1317 168
EaeI y'GGCC_r
Cuts at: 0 1035 1485
Size: 1035 450
EarI CTCTTCn'nnn_
Cuts at: 0 683 920 1485
Size: 683 237 565
Fragments arranged by size:
683 565 237
EcoICRI GAG'CTC
Cuts at: 0 297 1485
Size: 297 1188
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 283 364 464 694 1485
Size: 283 81 100 230 791
Fragments arranged by size:
791 283 230 100 81
EcoO109I rG'GnC_Cy
Cuts at: 0 37 348 1006 1050 1226 1485
Size: 37 311 658 44 176 259
Fragments arranged by size:
658 311 259 176 44 37
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 430 675 1191 1485
Size: 430 245 516 294
Fragments arranged by size:
516 430 294 245
HaeII r_GCGC'y
Cuts at: 0 1173 1485
Size: 1173 312
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 622 654 723 755 895 927 1485
Size: 622 32 69 32 140 32 558
Fragments arranged by size:
622 558 140 69 32 32 32
HincII GTy'rAC
Cuts at: 0 393 1485
Size: 393 1092
HindIII A'AGCT_T
Cuts at: 0 836 1485
Size: 836 649
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 146 1144 1485
Size: 146 998 341
Fragments arranged by size:
998 341 146
MscI TGG'CCA
Cuts at: 0 1037 1485
Size: 1037 448
MslI CAynn'nnrTG
Cuts at: 0 21 259 949 1031 1485
Size: 21 238 690 82 454
Fragments arranged by size:
690 454 238 82 21
MspA1I CmG'CkG
Cuts at: 0 152 851 1434 1485
Size: 152 699 583 51
Fragments arranged by size:
699 583 152 51
NsiI A_TGCA'T
Cuts at: 0 518 1485
Size: 518 967
NspI r_CATG'y
Cuts at: 0 258 1070 1236 1485
Size: 258 812 166 249
Fragments arranged by size:
812 258 249 166
PasI CC'CwG_GG
Cuts at: 0 52 560 1485
Size: 52 508 925
Fragments arranged by size:
925 508 52
PciI A'CATG_T
Cuts at: 0 1232 1485
Size: 1232 253
PflMI CCAn_nnn'nTGG
Cuts at: 0 380 491 1485
Size: 380 111 994
Fragments arranged by size:
994 380 111
PfoI T'CCnGG_A
Cuts at: 0 248 577 1485
Size: 248 329 908
Fragments arranged by size:
908 329 248
PpuMI rG'GwC_Cy
Cuts at: 0 37 348 1485
Size: 37 311 1137
Fragments arranged by size:
1137 311 37
PspOMI G'GGCC_C
Cuts at: 0 1014 1485
Size: 1014 471
PstI C_TGCA'G
Cuts at: 0 1333 1485
Size: 1333 152
PvuII CAG'CTG
Cuts at: 0 152 1434 1485
Size: 152 1282 51
Fragments arranged by size:
1282 152 51
SacI G_AGCT'C
Cuts at: 0 299 1485
Size: 299 1186
SapI GCTCTTCn'nnn_
Cuts at: 0 920 1485
Size: 920 565
SfcI C'TryA_G
Cuts at: 0 386 1329 1485
Size: 386 943 156
Fragments arranged by size:
943 386 156
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1013 1485
Size: 1013 472
SmlI C'TyrA_G
Cuts at: 0 932 1485
Size: 932 553
StuI AGG'CCT
Cuts at: 0 1423 1485
Size: 1423 62
StyI C'CwwG_G
Cuts at: 0 659 1046 1485
Size: 659 387 439
Fragments arranged by size:
659 439 387
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1043 1393 1485
Size: 1043 350 92
Fragments arranged by size:
1043 350 92
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 657 1485
Size: 657 828
XmnI GAAnn'nnTTC
Cuts at: 0 1171 1485
Size: 1171 314
ZraI GAC'GTC
Cuts at: 0 1242 1485
Size: 1242 243
Enzymes that do cut:
AatII AclI AcuI AfeI AflIII AleI AlwNI ApaI
ApoI BamHI BanII BbsI BbvCI BfrBI BglI BlpI
Bme1580I BmgBI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BssSI BstAPI BstEII BstXI BstYI BtgI
DraI EaeI EarI EcoICRI Eco57MI EcoO109I FalI HaeII
Hin4I HincII HindIII MmeI MscI MslI MspA1I NsiI
NspI PasI PciI PflMI PfoI PpuMI PspOMI PstI
PvuII SacI SapI SfcI SfiI SmlI StuI StyI
TaqII XcmI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AflII AgeI AhdI AlfI AloI
ApaLI AscI AseI AsiSI AvaI AvrII BaeI BanI
BbeI BcgI BciVI BclI BglII BmrI BmtI BplI
BsaAI BsaBI BsiEI BsiWI BsmI BsrBI BsrDI BsrFI
BsrGI BssHII BstBI BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DraIII DrdI EagI EciI EcoNI EcoRI EcoRV
FseI FspI FspAI HpaI KasI KpnI MfeI MluI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
PacI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI SwaI TatI TstI Tth111I XbaI XhoI XmaI