Restriction map for
pFN21AE3213
(Linear) (Six-base) MAPSORT of: KIEE3213.seq Check: 1348 from: 1 to: 1473
KIEE3213.orf
With 182 enzymes: SgfI *
December 27, 2011 16:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 161 1473
Size: 161 1312
AccI GT'mk_AC
Cuts at: 0 1423 1473
Size: 1423 50
Acc65I G'GTAC_C
Cuts at: 0 35 1473
Size: 35 1438
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 730 1473
Size: 124 606 743
Fragments arranged by size:
743 606 124
AfeI AGC'GCT
Cuts at: 0 82 934 1473
Size: 82 852 539
Fragments arranged by size:
852 539 82
AleI CACnn'nnGTG
Cuts at: 0 799 1473
Size: 799 674
AlwNI CAG_nnn'CTG
Cuts at: 0 1116 1473
Size: 1116 357
ApaI G_GGCC'C
Cuts at: 0 752 868 1473
Size: 752 116 605
Fragments arranged by size:
752 605 116
ApaLI G'TGCA_C
Cuts at: 0 784 1473
Size: 784 689
AvaI C'yCGr_G
Cuts at: 0 438 853 936 1057 1473
Size: 438 415 83 121 416
Fragments arranged by size:
438 416 415 121 83
BanI G'GyrC_C
Cuts at: 0 35 835 1473
Size: 35 800 638
Fragments arranged by size:
800 638 35
BanII G_rGCy'C
Cuts at: 0 676 752 854 868 1473
Size: 676 76 102 14 605
Fragments arranged by size:
676 605 102 76 14
BbsI GAAGACnn'nnnn_
Cuts at: 0 133 949 1473
Size: 133 816 524
Fragments arranged by size:
816 524 133
BciVI GTATCCnnnnn_n'
Cuts at: 0 309 1473
Size: 309 1164
BclI T'GATC_A
Cuts at: 0 770 791 1254 1473
Size: 770 21 463 219
Fragments arranged by size:
770 463 219 21
BglI GCCn_nnn'nGGC
Cuts at: 0 833 1473
Size: 833 640
Bme1580I G_kGCm'C
Cuts at: 0 752 788 868 1473
Size: 752 36 80 605
Fragments arranged by size:
752 605 80 36
BmrI ACTGGGnnnn_n'
Cuts at: 0 521 1473
Size: 521 952
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 708 958 1473
Size: 708 250 515
Fragments arranged by size:
708 515 250
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 484 1309 1473
Size: 484 825 164
Fragments arranged by size:
825 484 164
BsaAI yAC'GTr
Cuts at: 0 789 1399 1473
Size: 789 610 74
Fragments arranged by size:
789 610 74
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1075 1473
Size: 1075 398
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 741 1000 1192 1473
Size: 741 259 192 281
Fragments arranged by size:
741 281 259 192
BsiEI CG_ry'CG
Cuts at: 0 391 1473
Size: 391 1082
BsiHKAI G_wGCw'C
Cuts at: 0 386 788 1473
Size: 386 402 685
Fragments arranged by size:
685 402 386
BsmI GAATG_Cn'
Cuts at: 0 581 1340 1473
Size: 581 759 133
Fragments arranged by size:
759 581 133
Bsp1286I G_dGCh'C
Cuts at: 0 386 676 752 788 854 868 1473
Size: 386 290 76 36 66 14 605
Fragments arranged by size:
605 386 290 76 66 36 14
BspHI T'CATG_A
Cuts at: 0 185 1214 1473
Size: 185 1029 259
Fragments arranged by size:
1029 259 185
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 161 1001 1473
Size: 161 840 472
Fragments arranged by size:
840 472 161
BsrFI r'CCGG_y
Cuts at: 0 386 1473
Size: 386 1087
BsrGI T'GTAC_A
Cuts at: 0 962 1022 1473
Size: 962 60 451
Fragments arranged by size:
962 451 60
BstEII G'GTnAC_C
Cuts at: 0 941 1473
Size: 941 532
BstXI CCAn_nnnn'nTGG
Cuts at: 0 738 1473
Size: 738 735
Bsu36I CC'TnA_GG
Cuts at: 0 860 1473
Size: 860 613
BtgI C'CryG_G
Cuts at: 0 39 677 1473
Size: 39 638 796
Fragments arranged by size:
796 638 39
BtsI GCAGTG_nn'
Cuts at: 0 753 1473
Size: 753 720
DraI TTT'AAA
Cuts at: 0 269 1473
Size: 269 1204
EaeI y'GGCC_r
Cuts at: 0 388 740 1473
Size: 388 352 733
Fragments arranged by size:
733 388 352
EagI C'GGCC_G
Cuts at: 0 388 1473
Size: 388 1085
EarI CTCTTCn'nnn_
Cuts at: 0 131 586 1043 1473
Size: 131 455 457 430
Fragments arranged by size:
457 455 430 131
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 708 730 958 1473
Size: 124 584 22 228 515
Fragments arranged by size:
584 515 228 124 22
EcoO109I rG'GnC_Cy
Cuts at: 0 309 749 864 1473
Size: 309 440 115 609
Fragments arranged by size:
609 440 309 115
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 735 1473
Size: 735 738
HaeII r_GCGC'y
Cuts at: 0 84 936 1473
Size: 84 852 537
Fragments arranged by size:
852 537 84
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 541 573 1234 1266 1410 1442 1473
Size: 541 32 661 32 144 32 31
Fragments arranged by size:
661 541 144 32 32 32 31
HincII GTy'rAC
Cuts at: 0 1377 1424 1473
Size: 1377 47 49
Fragments arranged by size:
1377 49 47
KpnI G_GTAC'C
Cuts at: 0 39 1473
Size: 39 1434
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 755 968 1473
Size: 755 213 505
Fragments arranged by size:
755 505 213
MscI TGG'CCA
Cuts at: 0 742 1473
Size: 742 731
MslI CAynn'nnrTG
Cuts at: 0 799 1473
Size: 799 674
NspI r_CATG'y
Cuts at: 0 489 1473
Size: 489 984
PflMI CCAn_nnn'nTGG
Cuts at: 0 421 1473
Size: 421 1052
PmlI CAC'GTG
Cuts at: 0 789 1473
Size: 789 684
PpuMI rG'GwC_Cy
Cuts at: 0 309 1473
Size: 309 1164
PshAI GACnn'nnGTC
Cuts at: 0 291 1473
Size: 291 1182
PspOMI G'GGCC_C
Cuts at: 0 748 864 1473
Size: 748 116 609
Fragments arranged by size:
748 609 116
PstI C_TGCA'G
Cuts at: 0 158 605 1241 1473
Size: 158 447 636 232
Fragments arranged by size:
636 447 232 158
SalI G'TCGA_C
Cuts at: 0 1422 1473
Size: 1422 51
SfcI C'TryA_G
Cuts at: 0 154 601 1237 1473
Size: 154 447 636 236
Fragments arranged by size:
636 447 236 154
SmlI C'TyrA_G
Cuts at: 0 438 499 1324 1473
Size: 438 61 825 149
Fragments arranged by size:
825 438 149 61
SphI G_CATG'C
Cuts at: 0 489 1473
Size: 489 984
StuI AGG'CCT
Cuts at: 0 1010 1263 1473
Size: 1010 253 210
Fragments arranged by size:
1010 253 210
StyI C'CwwG_G
Cuts at: 0 912 1199 1473
Size: 912 287 274
Fragments arranged by size:
912 287 274
TatI w'GTAC_w
Cuts at: 0 962 1022 1473
Size: 962 60 451
Fragments arranged by size:
962 451 60
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 828 860 1473
Size: 828 32 613
Fragments arranged by size:
828 613 32
XhoI C'TCGA_G
Cuts at: 0 438 1473
Size: 438 1035
XmnI GAAnn'nnTTC
Cuts at: 0 715 1052 1473
Size: 715 337 421
Fragments arranged by size:
715 421 337
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AleI AlwNI ApaI
ApaLI AvaI BanI BanII BbsI BciVI BclI BglI
Bme1580I BmrI BpmI BpuEI BsaAI BseRI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrFI BsrGI BstEII
BstXI Bsu36I BtgI BtsI DraI EaeI EagI EarI
Eco57MI EcoO109I FalI HaeII Hin4I HincII KpnI MmeI
MscI MslI NspI PflMI PmlI PpuMI PshAI PspOMI
PstI SalI SfcI SmlI SphI StuI StyI TatI
TstI XhoI XmnI
Enzymes that do not cut:
AatII AclI AflII AflIII AgeI AhdI AlfI AloI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BbvCI BcgI BfrBI BglII BlpI BmgBI BmtI BplI
Bpu10I BsaI BsaBI BsaHI BsaWI BsaXI BseYI BsiWI
BsmBI BspEI BsrBI BsrDI BssHII BssSI BstAPI BstBI
BstYI BstZ17I BtgZI ClaI CspCI DraIII DrdI EciI
EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI HindIII
HpaI KasI MfeI MluI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PfoI PmeI PpiI PsiI PspXI PsrI PvuI
PvuII RsrII SacI SacII SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SrfI SspI SwaI TaqII Tth111I XbaI XcmI XmaI
ZraI