Restriction map for
pFN21AE3212
(Linear) (Six-base) MAPSORT of: KIEE3212.seq Check: 1496 from: 1 to: 1437
KIEE3212.orf
With 182 enzymes: SgfI *
July 3, 2012 17:27 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 609 1437
Size: 609 828
AflIII A'CryG_T
Cuts at: 0 944 1437
Size: 944 493
BamHI G'GATC_C
Cuts at: 0 735 1437
Size: 735 702
BanI G'GyrC_C
Cuts at: 0 332 1437
Size: 332 1105
BanII G_rGCy'C
Cuts at: 0 49 929 961 1437
Size: 49 880 32 476
Fragments arranged by size:
880 476 49 32
BbsI GAAGACnn'nnnn_
Cuts at: 0 581 980 1437
Size: 581 399 457
Fragments arranged by size:
581 457 399
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1144 1178 1437
Size: 1144 34 259
Fragments arranged by size:
1144 259 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 814 1437
Size: 814 623
BglII A'GATC_T
Cuts at: 0 1013 1437
Size: 1013 424
Bme1580I G_kGCm'C
Cuts at: 0 799 1370 1437
Size: 799 571 67
Fragments arranged by size:
799 571 67
BmrI ACTGGGnnnn_n'
Cuts at: 0 735 1437
Size: 735 702
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 346 428 525 1393 1437
Size: 82 264 82 97 868 44
Fragments arranged by size:
868 264 97 82 82 44
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 975 1437
Size: 975 462
BsaAI yAC'GTr
Cuts at: 0 947 965 1437
Size: 947 18 472
Fragments arranged by size:
947 472 18
BsaWI w'CCGG_w
Cuts at: 0 377 1437
Size: 377 1060
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1039 1069 1437
Size: 1039 30 368
Fragments arranged by size:
1039 368 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1171 1378 1437
Size: 1171 207 59
Fragments arranged by size:
1171 207 59
BsiHKAI G_wGCw'C
Cuts at: 0 929 961 1437
Size: 929 32 476
Fragments arranged by size:
929 476 32
BsmI GAATG_Cn'
Cuts at: 0 1407 1437
Size: 1407 30
Bsp1286I G_dGCh'C
Cuts at: 0 49 799 929 961 1370 1437
Size: 49 750 130 32 409 67
Fragments arranged by size:
750 409 130 67 49 32
BspHI T'CATG_A
Cuts at: 0 1316 1437
Size: 1316 121
BsrBI CCG'CTC
Cuts at: 0 6 1107 1437
Size: 6 1101 330
Fragments arranged by size:
1101 330 6
BstXI CCAn_nnnn'nTGG
Cuts at: 0 600 1437
Size: 600 837
BstYI r'GATC_y
Cuts at: 0 735 1013 1437
Size: 735 278 424
Fragments arranged by size:
735 424 278
BtgI C'CryG_G
Cuts at: 0 513 1437
Size: 513 924
BtsI GCAGTG_nn'
Cuts at: 0 1037 1437
Size: 1037 400
ClaI AT'CG_AT
Cuts at: 0 282 1437
Size: 282 1155
EaeI y'GGCC_r
Cuts at: 0 696 866 1437
Size: 696 170 571
Fragments arranged by size:
696 571 170
EarI CTCTTCn'nnn_
Cuts at: 0 485 1437
Size: 485 952
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 74 1437
Size: 74 1363
EcoICRI GAG'CTC
Cuts at: 0 927 959 1437
Size: 927 32 478
Fragments arranged by size:
927 478 32
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 346 428 525 609 1393 1437
Size: 82 264 82 97 84 784 44
Fragments arranged by size:
784 264 97 84 82 82 44
EcoNI CCTnn'n_nnAGG
Cuts at: 0 26 1393 1437
Size: 26 1367 44
Fragments arranged by size:
1367 44 26
EcoO109I rG'GnC_Cy
Cuts at: 0 780 1437
Size: 780 657
EcoRV GAT'ATC
Cuts at: 0 443 1200 1437
Size: 443 757 237
Fragments arranged by size:
757 443 237
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1032 1437
Size: 1032 405
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 727 759 1437
Size: 727 32 678
Fragments arranged by size:
727 678 32
MfeI C'AATT_G
Cuts at: 0 1234 1437
Size: 1234 203
MscI TGG'CCA
Cuts at: 0 698 1437
Size: 698 739
MslI CAynn'nnrTG
Cuts at: 0 643 802 1437
Size: 643 159 635
Fragments arranged by size:
643 635 159
MspA1I CmG'CkG
Cuts at: 0 765 1227 1380 1437
Size: 765 462 153 57
Fragments arranged by size:
765 462 153 57
NcoI C'CATG_G
Cuts at: 0 513 1437
Size: 513 924
NspI r_CATG'y
Cuts at: 0 405 1437
Size: 405 1032
PasI CC'CwG_GG
Cuts at: 0 538 1437
Size: 538 899
PflMI CCAn_nnn'nTGG
Cuts at: 0 503 1437
Size: 503 934
PfoI T'CCnGG_A
Cuts at: 0 59 1437
Size: 59 1378
PmlI CAC'GTG
Cuts at: 0 947 1437
Size: 947 490
PpuMI rG'GwC_Cy
Cuts at: 0 780 1437
Size: 780 657
PvuII CAG'CTG
Cuts at: 0 765 1227 1380 1437
Size: 765 462 153 57
Fragments arranged by size:
765 462 153 57
SacI G_AGCT'C
Cuts at: 0 929 961 1437
Size: 929 32 476
Fragments arranged by size:
929 476 32
ScaI AGT'ACT
Cuts at: 0 340 1048 1437
Size: 340 708 389
Fragments arranged by size:
708 389 340
SfcI C'TryA_G
Cuts at: 0 200 1437
Size: 200 1237
SmlI C'TyrA_G
Cuts at: 0 954 1437
Size: 954 483
SphI G_CATG'C
Cuts at: 0 405 1437
Size: 405 1032
StyI C'CwwG_G
Cuts at: 0 513 1437
Size: 513 924
TatI w'GTAC_w
Cuts at: 0 338 635 1046 1437
Size: 338 297 411 391
Fragments arranged by size:
411 391 338 297
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 635 667 812 844 931 963 1343 1375
Size: 635 32 145 32 87 32 380 32
Cuts at: 1375 1437
Size: 62
Fragments arranged by size:
635 380 145 87 62 32 32 32
32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 535 1437
Size: 535 902
XmnI GAAnn'nnTTC
Cuts at: 0 613 1437
Size: 613 824
Enzymes that do cut:
AcuI AflIII BamHI BanI BanII BbsI BcgI BciVI
BglII Bme1580I BmrI BpmI BpuEI BsaAI BsaWI BsaXI
BseRI BsiHKAI BsmI Bsp1286I BspHI BsrBI BstXI BstYI
BtgI BtsI ClaI EaeI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRV FalI Hin4I MfeI MscI MslI
MspA1I NcoI NspI PasI PflMI PfoI PmlI PpuMI
PvuII SacI ScaI SfcI SmlI SphI StyI TatI
TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI AvrII BaeI BbeI BbvCI
BclI BfrBI BglI BlpI BmgBI BmtI BplI Bpu10I
BsaI BsaBI BsaHI BseYI BsgI BsiEI BsiWI BsmBI
BspEI BspMI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstZ17I Bsu36I BtgZI CspCI DraI DraIII
DrdI EagI EcoRI FseI FspI FspAI HaeII HincII
HindIII HpaI KasI KpnI MluI MmeI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI PacI PciI
PmeI PpiI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI RsrII SacII SalI SanDI SapI SbfI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
SspI StuI SwaI TaqII Tth111I XbaI XhoI XmaI
ZraI