Restriction map for
pFN21AE3211
(Linear) (Six-base) MAPSORT of: KIEE3211.seq Check: 6167 from: 1 to: 1437
KIEE3211.orf
With 182 enzymes: SgfI *
March 22, 2012 16:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1097 1437
Size: 1097 340
AatII G_ACGT'C
Cuts at: 0 746 1437
Size: 746 691
Acc65I G'GTAC_C
Cuts at: 0 1202 1437
Size: 1202 235
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 401 1295 1437
Size: 401 894 142
Fragments arranged by size:
894 401 142
AflIII A'CryG_T
Cuts at: 0 430 477 1437
Size: 430 47 960
Fragments arranged by size:
960 430 47
AlwNI CAG_nnn'CTG
Cuts at: 0 444 459 654 759 982 1017 1192 1437
Size: 444 15 195 105 223 35 175 245
Fragments arranged by size:
444 245 223 195 175 105 35 15
AseI AT'TA_AT
Cuts at: 0 1412 1437
Size: 1412 25
AvaI C'yCGr_G
Cuts at: 0 34 804 820 1437
Size: 34 770 16 617
Fragments arranged by size:
770 617 34 16
BamHI G'GATC_C
Cuts at: 0 449 1437
Size: 449 988
BanI G'GyrC_C
Cuts at: 0 615 1202 1437
Size: 615 587 235
Fragments arranged by size:
615 587 235
BanII G_rGCy'C
Cuts at: 0 947 1437
Size: 947 490
BbsI GAAGACnn'nnnn_
Cuts at: 0 337 738 1377 1437
Size: 337 401 639 60
Fragments arranged by size:
639 401 337 60
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 795 829 1437
Size: 795 34 608
Fragments arranged by size:
795 608 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 37 1437
Size: 37 1400
BglI GCCn_nnn'nGGC
Cuts at: 0 862 1437
Size: 862 575
BlpI GC'TnA_GC
Cuts at: 0 1006 1437
Size: 1006 431
Bme1580I G_kGCm'C
Cuts at: 0 173 1437
Size: 173 1264
BmgBI CAC'GTC
Cuts at: 0 480 1437
Size: 480 957
BmrI ACTGGGnnnn_n'
Cuts at: 0 39 1202 1437
Size: 39 1163 235
Fragments arranged by size:
1163 235 39
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 645 1437
Size: 645 792
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 283 482 572 658 679 1437
Size: 112 171 199 90 86 21 758
Fragments arranged by size:
758 199 171 112 90 86 21
BsaHI Gr'CG_yC
Cuts at: 0 5 743 1061 1437
Size: 5 738 318 376
Fragments arranged by size:
738 376 318 5
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1321 1351 1437
Size: 1321 30 86
Fragments arranged by size:
1321 86 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 70 244 982 1437
Size: 70 174 738 455
Fragments arranged by size:
738 455 174 70
BseYI C'CCAG_C
Cuts at: 0 340 1046 1437
Size: 340 706 391
Fragments arranged by size:
706 391 340
BsiHKAI G_wGCw'C
Cuts at: 0 498 947 1437
Size: 498 449 490
Fragments arranged by size:
498 490 449
BsmBI CGTCTCn'nnnn_
Cuts at: 0 421 613 634 1437
Size: 421 192 21 803
Fragments arranged by size:
803 421 192 21
Bsp1286I G_dGCh'C
Cuts at: 0 173 498 947 1437
Size: 173 325 449 490
Fragments arranged by size:
490 449 325 173
BspHI T'CATG_A
Cuts at: 0 134 995 1437
Size: 134 861 442
Fragments arranged by size:
861 442 134
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1097 1272 1437
Size: 1097 175 165
Fragments arranged by size:
1097 175 165
BsrBI CCG'CTC
Cuts at: 0 1161 1437
Size: 1161 276
BsrDI GCAATG_nn'
Cuts at: 0 227 1437
Size: 227 1210
BsrGI T'GTAC_A
Cuts at: 0 1214 1437
Size: 1214 223
BssHII G'CGCG_C
Cuts at: 0 344 1437
Size: 344 1093
BstAPI GCAn_nnn'nTGC
Cuts at: 0 87 1437
Size: 87 1350
BstBI TT'CG_AA
Cuts at: 0 1388 1437
Size: 1388 49
BstYI r'GATC_y
Cuts at: 0 120 449 1238 1437
Size: 120 329 789 199
Fragments arranged by size:
789 329 199 120
Bsu36I CC'TnA_GG
Cuts at: 0 201 1437
Size: 201 1236
BtgI C'CryG_G
Cuts at: 0 1066 1232 1437
Size: 1066 166 205
Fragments arranged by size:
1066 205 166
BtsI GCAGTG_nn'
Cuts at: 0 840 1437
Size: 840 597
EaeI y'GGCC_r
Cuts at: 0 296 580 1346 1437
Size: 296 284 766 91
Fragments arranged by size:
766 296 284 91
EarI CTCTTCn'nnn_
Cuts at: 0 377 526 1271 1437
Size: 377 149 745 166
Fragments arranged by size:
745 377 166 149
EcoICRI GAG'CTC
Cuts at: 0 945 1437
Size: 945 492
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 283 401 482 572 658 679 1295
Size: 112 171 118 81 90 86 21 616
Cuts at: 1295 1437
Size: 142
Fragments arranged by size:
616 171 142 118 112 90 86 81
21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 997 1078 1437
Size: 997 81 359
Fragments arranged by size:
997 359 81
EcoO109I rG'GnC_Cy
Cuts at: 0 1071 1371 1437
Size: 1071 300 66
Fragments arranged by size:
1071 300 66
HaeII r_GCGC'y
Cuts at: 0 1017 1437
Size: 1017 420
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1230 1262 1398 1430 1437
Size: 1230 32 136 32 7
Fragments arranged by size:
1230 136 32 32 7
KpnI G_GTAC'C
Cuts at: 0 1206 1437
Size: 1206 231
MluI A'CGCG_T
Cuts at: 0 430 1437
Size: 430 1007
MscI TGG'CCA
Cuts at: 0 582 1437
Size: 582 855
MslI CAynn'nnrTG
Cuts at: 0 1000 1437
Size: 1000 437
MspA1I CmG'CkG
Cuts at: 0 360 1068 1321 1437
Size: 360 708 253 116
Fragments arranged by size:
708 360 253 116
NcoI C'CATG_G
Cuts at: 0 1232 1437
Size: 1232 205
NspI r_CATG'y
Cuts at: 0 90 1437
Size: 90 1347
PasI CC'CwG_GG
Cuts at: 0 459 1437
Size: 459 978
PflMI CCAn_nnn'nTGG
Cuts at: 0 459 1017 1192 1437
Size: 459 558 175 245
Fragments arranged by size:
558 459 245 175
PpuMI rG'GwC_Cy
Cuts at: 0 1071 1371 1437
Size: 1071 300 66
Fragments arranged by size:
1071 300 66
PshAI GACnn'nnGTC
Cuts at: 0 766 1437
Size: 766 671
PstI C_TGCA'G
Cuts at: 0 851 1259 1437
Size: 851 408 178
Fragments arranged by size:
851 408 178
PvuII CAG'CTG
Cuts at: 0 360 1321 1437
Size: 360 961 116
Fragments arranged by size:
961 360 116
SacI G_AGCT'C
Cuts at: 0 947 1437
Size: 947 490
SacII CC_GC'GG
Cuts at: 0 1069 1437
Size: 1069 368
SapI GCTCTTCn'nnn_
Cuts at: 0 1271 1437
Size: 1271 166
SfcI C'TryA_G
Cuts at: 0 847 1255 1308 1437
Size: 847 408 53 129
Fragments arranged by size:
847 408 129 53
SspI AAT'ATT
Cuts at: 0 185 1437
Size: 185 1252
StyI C'CwwG_G
Cuts at: 0 1232 1437
Size: 1232 205
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 486 1038 1437
Size: 486 552 399
Fragments arranged by size:
552 486 399
TatI w'GTAC_w
Cuts at: 0 1214 1437
Size: 1214 223
Tth111I GACn'n_nGTC
Cuts at: 0 505 1437
Size: 505 932
ZraI GAC'GTC
Cuts at: 0 744 1437
Size: 744 693
Enzymes that do cut:
AarI AatII Acc65I AcuI AflIII AlwNI AseI AvaI
BamHI BanI BanII BbsI BcgI BciVI BglI BlpI
Bme1580I BmgBI BmrI BplI BpmI BsaHI BsaXI BseRI
BseYI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrGI BssHII BstAPI BstBI BstYI Bsu36I BtgI BtsI
EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I
KpnI MluI MscI MslI MspA1I NcoI NspI PasI
PflMI PpuMI PshAI PstI PvuII SacI SacII SapI
SfcI SspI StyI TaqII TatI Tth111I ZraI
Enzymes that do not cut:
AccI AclI AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI ApaLI ApoI AscI AsiSI AvrII BaeI
BbeI BbvCI BclI BfrBI BglII BmtI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaWI BsgI BsiEI BsiWI BsmI
BspEI BsrFI BssSI BstEII BstXI BstZ17I BtgZI ClaI
CspCI DraI DraIII DrdI EagI EciI EcoRI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI KasI
MfeI MmeI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PciI PfoI PmeI PmlI PpiI
PsiI PspOMI PspXI PsrI PvuI RsrII SalI SanDI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI StuI SwaI TstI
XbaI XcmI XhoI XmaI XmnI