Restriction map for
pFN21AE3207
(Linear) (Six-base) MAPSORT of: KIEE3207.seq Check: 3177 from: 1 to: 1431
KIEE3207.orf
With 182 enzymes: SgfI *
July 3, 2012 17:26 ..
AflII C'TTAA_G
Cuts at: 0 1048 1431
Size: 1048 383
AhdI GACnn_n'nnGTC
Cuts at: 0 239 1431
Size: 239 1192
AleI CACnn'nnGTG
Cuts at: 0 881 1431
Size: 881 550
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 213 1431
Size: 213 1218
AlwNI CAG_nnn'CTG
Cuts at: 0 600 769 1431
Size: 600 169 662
Fragments arranged by size:
662 600 169
ApoI r'AATT_y
Cuts at: 0 499 1115 1208 1431
Size: 499 616 93 223
Fragments arranged by size:
616 499 223 93
AseI AT'TA_AT
Cuts at: 0 704 1431
Size: 704 727
AvaI C'yCGr_G
Cuts at: 0 296 304 312 1431
Size: 296 8 8 1119
Fragments arranged by size:
1119 296 8 8
BamHI G'GATC_C
Cuts at: 0 352 360 1431
Size: 352 8 1071
Fragments arranged by size:
1071 352 8
BanI G'GyrC_C
Cuts at: 0 14 1431
Size: 14 1417
BanII G_rGCy'C
Cuts at: 0 53 92 105 305 1431
Size: 53 39 13 200 1126
Fragments arranged by size:
1126 200 53 39 13
BbsI GAAGACnn'nnnn_
Cuts at: 0 656 971 1431
Size: 656 315 460
Fragments arranged by size:
656 460 315
BbvCI CC'TCA_GC
Cuts at: 0 631 1431
Size: 631 800
BmgBI CAC'GTC
Cuts at: 0 414 1431
Size: 414 1017
BmrI ACTGGGnnnn_n'
Cuts at: 0 378 491 558 1431
Size: 378 113 67 873
Fragments arranged by size:
873 378 113 67
Bpu10I CC'TnA_GC
Cuts at: 0 631 762 1431
Size: 631 131 669
Fragments arranged by size:
669 631 131
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1247 1431
Size: 1247 184
BsaI GGTCTCn'nnnn_
Cuts at: 0 128 314 438 1431
Size: 128 186 124 993
Fragments arranged by size:
993 186 128 124
BsaBI GATnn'nnATC
Cuts at: 0 727 1431
Size: 727 704
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 618 648 1431
Size: 618 30 783
Fragments arranged by size:
783 618 30
BseYI C'CCAG_C
Cuts at: 0 457 1431
Size: 457 974
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 1431
Size: 55 1376
BsiHKAI G_wGCw'C
Cuts at: 0 105 161 305 1431
Size: 105 56 144 1126
Fragments arranged by size:
1126 144 105 56
BsmI GAATG_Cn'
Cuts at: 0 1320 1431
Size: 1320 111
BsmBI CGTCTCn'nnnn_
Cuts at: 0 420 1431
Size: 420 1011
Bsp1286I G_dGCh'C
Cuts at: 0 53 92 105 161 305 1431
Size: 53 39 13 56 144 1126
Fragments arranged by size:
1126 144 56 53 39 13
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 110 146 1431
Size: 110 36 1285
Fragments arranged by size:
1285 110 36
BsrBI CCG'CTC
Cuts at: 0 317 1431
Size: 317 1114
BsrFI r'CCGG_y
Cuts at: 0 329 1431
Size: 329 1102
BsrGI T'GTAC_A
Cuts at: 0 1248 1431
Size: 1248 183
BssSI C'ACGA_G
Cuts at: 0 160 1431
Size: 160 1271
BstAPI GCAn_nnn'nTGC
Cuts at: 0 627 1431
Size: 627 804
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1370 1431
Size: 1370 61
BstYI r'GATC_y
Cuts at: 0 352 360 1431
Size: 352 8 1071
Fragments arranged by size:
1071 352 8
BtgI C'CryG_G
Cuts at: 0 18 1431
Size: 18 1413
EaeI y'GGCC_r
Cuts at: 0 591 916 1431
Size: 591 325 515
Fragments arranged by size:
591 515 325
EarI CTCTTCn'nnn_
Cuts at: 0 278 398 905 1431
Size: 278 120 507 526
Fragments arranged by size:
526 507 278 120
EcoICRI GAG'CTC
Cuts at: 0 103 303 1431
Size: 103 200 1128
Fragments arranged by size:
1128 200 103
EcoNI CCTnn'n_nnAGG
Cuts at: 0 43 82 1431
Size: 43 39 1349
Fragments arranged by size:
1349 43 39
EcoO109I rG'GnC_Cy
Cuts at: 0 122 1431
Size: 122 1309
EcoRI G'AATT_C
Cuts at: 0 1115 1431
Size: 1115 316
EcoRV GAT'ATC
Cuts at: 0 729 1431
Size: 729 702
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 225 257 958 990 1431
Size: 225 32 701 32 441
Fragments arranged by size:
701 441 225 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 783 1431
Size: 783 648
MscI TGG'CCA
Cuts at: 0 918 1431
Size: 918 513
MslI CAynn'nnrTG
Cuts at: 0 881 1058 1431
Size: 881 177 373
Fragments arranged by size:
881 373 177
MspA1I CmG'CkG
Cuts at: 0 20 1431
Size: 20 1411
NdeI CA'TA_TG
Cuts at: 0 1335 1431
Size: 1335 96
NspI r_CATG'y
Cuts at: 0 630 1431
Size: 630 801
PflMI CCAn_nnn'nTGG
Cuts at: 0 855 1431
Size: 855 576
PpuMI rG'GwC_Cy
Cuts at: 0 122 1431
Size: 122 1309
PspXI vC'TCGA_Gb
Cuts at: 0 304 312 1431
Size: 304 8 1119
Fragments arranged by size:
1119 304 8
SacI G_AGCT'C
Cuts at: 0 105 305 1431
Size: 105 200 1126
Fragments arranged by size:
1126 200 105
SacII CC_GC'GG
Cuts at: 0 21 1431
Size: 21 1410
SapI GCTCTTCn'nnn_
Cuts at: 0 398 1431
Size: 398 1033
SexAI A'CCwGG_T
Cuts at: 0 1377 1431
Size: 1377 54
SmlI C'TyrA_G
Cuts at: 0 296 304 312 1048 1262 1431
Size: 296 8 8 736 214 169
Fragments arranged by size:
736 296 214 169 8 8
StyI C'CwwG_G
Cuts at: 0 644 1431
Size: 644 787
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 95 1431
Size: 95 1336
TatI w'GTAC_w
Cuts at: 0 623 1248 1365 1431
Size: 623 625 117 66
Fragments arranged by size:
625 623 117 66
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 337 369 1431
Size: 337 32 1062
Fragments arranged by size:
1062 337 32
XhoI C'TCGA_G
Cuts at: 0 296 304 312 1431
Size: 296 8 8 1119
Fragments arranged by size:
1119 296 8 8
XmnI GAAnn'nnTTC
Cuts at: 0 60 526 1431
Size: 60 466 905
Fragments arranged by size:
905 466 60
Enzymes that do cut:
AflII AhdI AleI AlfI AlwNI ApoI AseI AvaI
BamHI BanI BanII BbsI BbvCI BmgBI BmrI Bpu10I
BpuEI BsaI BsaBI BseRI BseYI BsgI BsiHKAI BsmI
BsmBI Bsp1286I BspMI BsrBI BsrFI BsrGI BssSI BstAPI
BstXI BstYI BtgI EaeI EarI EcoICRI EcoNI EcoO109I
EcoRI EcoRV Hin4I MmeI MscI MslI MspA1I NdeI
NspI PflMI PpuMI PspXI SacI SacII SapI SexAI
SmlI StyI TaqII TatI TstI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AloI ApaI ApaLI AscI AsiSI AvrII BaeI
BbeI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmtI BplI BpmI BsaAI BsaHI BsaWI BsaXI
BsiEI BsiWI BspEI BspHI BsrDI BssHII BstBI BstEII
BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EagI EciI Eco57MI FalI FseI FspI FspAI
HaeII HincII HindIII HpaI KasI KpnI MfeI MluI
NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PsrI PstI PvuI PvuII RsrII SalI
SanDI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
Tth111I XbaI XcmI XmaI ZraI