Restriction map for
pFN21AE3206
(Linear) (Six-base) MAPSORT of: KIEE3206.seq Check: 1329 from: 1 to: 1428
KIEE3206.orf
With 182 enzymes: SgfI *
July 3, 2012 17:26 ..
Acc65I G'GTAC_C
Cuts at: 0 226 1428
Size: 226 1202
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 176 583 994 1187 1343 1428
Size: 176 407 411 193 156 85
Fragments arranged by size:
411 407 193 176 156 85
AflIII A'CryG_T
Cuts at: 0 328 1428
Size: 328 1100
AlwNI CAG_nnn'CTG
Cuts at: 0 264 580 1428
Size: 264 316 848
Fragments arranged by size:
848 316 264
ApaLI G'TGCA_C
Cuts at: 0 1025 1428
Size: 1025 403
ApoI r'AATT_y
Cuts at: 0 644 1208 1219 1428
Size: 644 564 11 209
Fragments arranged by size:
644 564 209 11
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 218 251 1428
Size: 218 33 1177
Fragments arranged by size:
1177 218 33
BanI G'GyrC_C
Cuts at: 0 226 1428
Size: 226 1202
BanII G_rGCy'C
Cuts at: 0 1178 1428
Size: 1178 250
BbsI GAAGACnn'nnnn_
Cuts at: 0 589 1428
Size: 589 839
BbvCI CC'TCA_GC
Cuts at: 0 1159 1428
Size: 1159 269
BclI T'GATC_A
Cuts at: 0 27 1428
Size: 27 1401
BglII A'GATC_T
Cuts at: 0 1041 1428
Size: 1041 387
Bme1580I G_kGCm'C
Cuts at: 0 713 1029 1428
Size: 713 316 399
Fragments arranged by size:
713 399 316
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1026 1428
Size: 1026 402
Bpu10I CC'TnA_GC
Cuts at: 0 1159 1177 1428
Size: 1159 18 251
Fragments arranged by size:
1159 251 18
BsaAI yAC'GTr
Cuts at: 0 118 329 1428
Size: 118 211 1099
Fragments arranged by size:
1099 211 118
BsaWI w'CCGG_w
Cuts at: 0 1257 1428
Size: 1257 171
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 462 1015 1428
Size: 462 553 413
Fragments arranged by size:
553 462 413
BsiHKAI G_wGCw'C
Cuts at: 0 720 1029 1178 1428
Size: 720 309 149 250
Fragments arranged by size:
720 309 250 149
BsmI GAATG_Cn'
Cuts at: 0 378 1428
Size: 378 1050
Bsp1286I G_dGCh'C
Cuts at: 0 713 720 1029 1178 1428
Size: 713 7 309 149 250
Fragments arranged by size:
713 309 250 149 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 559 1428
Size: 559 869
BsrDI GCAATG_nn'
Cuts at: 0 1304 1428
Size: 1304 124
BstXI CCAn_nnnn'nTGG
Cuts at: 0 333 1428
Size: 333 1095
BstYI r'GATC_y
Cuts at: 0 1041 1254 1428
Size: 1041 213 174
Fragments arranged by size:
1041 213 174
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1248 1428
Size: 1248 180
BtsI GCAGTG_nn'
Cuts at: 0 171 275 1428
Size: 171 104 1153
Fragments arranged by size:
1153 171 104
DraI TTT'AAA
Cuts at: 0 1037 1428
Size: 1037 391
DraIII CAC_nnn'GTG
Cuts at: 0 706 1428
Size: 706 722
EaeI y'GGCC_r
Cuts at: 0 1394 1428
Size: 1394 34
EarI CTCTTCn'nnn_
Cuts at: 0 1166 1428
Size: 1166 262
EcoICRI GAG'CTC
Cuts at: 0 1176 1428
Size: 1176 252
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 176 583 994 1187 1343 1428
Size: 176 407 411 193 156 85
Fragments arranged by size:
411 407 193 176 156 85
EcoRI G'AATT_C
Cuts at: 0 1208 1428
Size: 1208 220
HincII GTy'rAC
Cuts at: 0 45 1428
Size: 45 1383
HindIII A'AGCT_T
Cuts at: 0 453 1428
Size: 453 975
KpnI G_GTAC'C
Cuts at: 0 230 1428
Size: 230 1198
MscI TGG'CCA
Cuts at: 0 1396 1428
Size: 1396 32
MslI CAynn'nnrTG
Cuts at: 0 161 1428
Size: 161 1267
MspA1I CmG'CkG
Cuts at: 0 349 972 1363 1428
Size: 349 623 391 65
Fragments arranged by size:
623 391 349 65
NdeI CA'TA_TG
Cuts at: 0 1284 1428
Size: 1284 144
NspI r_CATG'y
Cuts at: 0 434 1428
Size: 434 994
PmlI CAC'GTG
Cuts at: 0 329 1428
Size: 329 1099
PvuII CAG'CTG
Cuts at: 0 349 972 1428
Size: 349 623 456
Fragments arranged by size:
623 456 349
SacI G_AGCT'C
Cuts at: 0 1178 1428
Size: 1178 250
SapI GCTCTTCn'nnn_
Cuts at: 0 1166 1428
Size: 1166 262
SexAI A'CCwGG_T
Cuts at: 0 841 1428
Size: 841 587
StyI C'CwwG_G
Cuts at: 0 86 422 1184 1428
Size: 86 336 762 244
Fragments arranged by size:
762 336 244 86
TatI w'GTAC_w
Cuts at: 0 107 697 1428
Size: 107 590 731
Fragments arranged by size:
731 590 107
Tth111I GACn'n_nGTC
Cuts at: 0 40 1428
Size: 40 1388
Enzymes that do cut:
Acc65I AcuI AflIII AlwNI ApaLI ApoI BaeI BanI
BanII BbsI BbvCI BclI BglII Bme1580I BplI Bpu10I
BsaAI BsaWI BseRI BsiHKAI BsmI Bsp1286I BspMI BsrDI
BstXI BstYI BtgZI BtsI DraI DraIII EaeI EarI
EcoICRI Eco57MI EcoRI HincII HindIII KpnI MscI MslI
MspA1I NdeI NspI PmlI PvuII SacI SapI SexAI
StyI TatI Tth111I
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaI AscI AseI AsiSI AvaI
AvrII BamHI BbeI BcgI BciVI BfrBI BglI BlpI
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaBI BsaHI
BsaXI BseYI BsgI BsiEI BsiWI BsmBI BspEI BspHI
BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstZ17I Bsu36I BtgI ClaI CspCI DrdI EagI EciI
EcoNI EcoO109I EcoRV FalI FseI FspI FspAI HaeII
Hin4I HpaI KasI MfeI MluI MmeI NaeI NarI
NcoI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI RsrII SacII SalI
SanDI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TaqII TstI XbaI XcmI XhoI XmaI XmnI
ZraI